Great B220/CD45R Antibody

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

B220/CD45R PE/Dazzle 594

Catalog Number:

103257

Mice are infected and splenocytes are collected, RBC lysed, Fc blocked, stained, and washed before performing flow. This antibody is used for plasma cell staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 10 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:400, 25 minutes

Tertiary Incubation

NA

Detection

LSRII

Results Summary

Great antibody. Use it for gating on B cells for plasma cell quantification. Reliable and great signal.

Additional Notes

None

Related Categories

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Summary

The Good

Reliable, Strong signal

The Bad

NA

The Bottom Line

Would highly recommend this antibody. We use it as equivalent of PE Texas Red.

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