CD44 PE Antibody Review

February 02, 2016

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

CD44 PE Antibody

Catalog Number:

338807

Mice are infected with virus. Spleens are collected, cells are RBC lysed, Fc blocked, stained, and washed before fixing and performing flow. We are looking at lymphocyte subpopulations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 10 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:400, 30 minutes

Tertiary Incubation

NA

Detection

LSRII

Results Summary

Robust and easy to optimize. We have used this antibody many time successfully.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Reliable

The Bad

None

The Bottom Line

Would recommend this antibody.

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