Good IgD Flow Cytometry Antibody

August 17, 2016

BlueskyReddit
Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Biolegend

Product Name:

IgD Pacific Blue Antibody

Catalog Number:

405711

Mice are latently infected with virus. Splenocytes are prepared and stained for flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

20 minutes, 1:100

Blocking Agent

Fc Block

Secondary Incubation

1:400, 30 minutes, ice

Tertiary Incubation

NA

Detection

LSRII

Results Summary

Antibody worked well for our experiments and gave expected signal for IgD.

Additional Notes

None

Image Gallery

Summary

The Good

Works well in a 4 color panel

The Bad

optimization took a couple tries

The Bottom Line

Would recommend this antibody

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