BioLegend
CD25 APC
101909
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Splenocytes are harvested from latently infected mice. They are RBC lysed, Fc blocked, stained for 30 minutes, and washed 2x before fixing them for flow.
Flow Cytometry
Splenocytes
NA
Fc Block 1:100, 10 minutes
1:400, 30 minutes
LSRII
The couple of times we have used this antibody, we did not get a robust signal as we would expect for infected mouse splenocytes. We have optimized at varying concentration, always fixing, so perhaps the signal would be more robust if you do not fix cells before flowing.
None
Inexpensive, ships fast
Unreliable
The reliability of this antibody may lie within experimental conditions. For our experimental set up we have not had the best luck with this antibody.