SP6800 Spectral Analyzer from Sony Corporation

Product Specs
ItemSP6800 Spectral Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberSP6800
Dimensions(WxDxH) 60.0 x 63.5 x 71.3 cm
DetectionFSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
CapacityTubes (5 mL)
Lasers2 or 3 lasers
Excitation405nm (60mW), 488nm (40mW), 638nm (60mW)
Parameters64 fluorescence channels
Data Throughput10,000 events/sec; 20,000 events/sec (max)

(1) Oncogenic PIK3CA mutations shape an immunoregulatory microenvironment in mosaic overgrowth disordersPNAS NexusMay 13, 2026Ilaria Galasso, Charles Bayard, Camille Blériot, Sophia Ladraa, Wan Ting Kong, Rubina Cassaca, Clément Hoguin, Sanela Protic, Jérôme Megret, Nicolas Goudin, et al.fluorescence-activated cell sorting (FACS) buffer (PBS containing 0.5–1% BSA). Samples were acquired using a Sony SP6800 Spectral Cell Analyzer and analyzed with Kaluza software (v2.1). Antibodies used in this study are listed

(2) IgA nephropathy-associated breast milk B cell alterationsFront ImmunolMay 8, 2026Katerina Zachova, Alica Cutkova, Petr Kosztyu, Yukako Ohyama, Kazuo Takahashi, Josef Zadrazil, Jiri Orsag, Nadezda Petejova, Jiri Mestecky, Milan Raska0.5% Tween-20 in PBS at 42 °C for 10 and 30 min, respectively, and analyzed by flow cytometry using SP6800 spectral analyzer (SONY Biotechnologies, Japan). Cell surface Gd-IgA1 staining After surface Fc receptor blocking and

(3) Modeling neonatal immune response to B. pertussis identifies early B cell activation and differentiationPLOS PathogensApril 22, 2026Soraya Matczak, Mirko Sadi, Pauline Leroux, Pauline Labé, Valérie Bouchez, Sandrine Schmutz, Valentina Libri, Valérie Seffer, Sophie Novault, Stevenn Volant, et al.steps, cells were either fixed in a solution of 2% paraformaldehyde and analyzed on a spectral analyzer (SP6800, Sony Biotechnology, San Jose, CA), or directly analyzed on a FACS AriaFusion (BD Biosciences). Data

(4) Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complexPLoS PathogApril 20, 2026Stefano Sellitto, Davide Caredio, Matteo Bimbati, Giovanni Mariutti, Martina Cerisoli, Lukas Frick, Vangelis Bouris, Carlos Omar Oueslati Morales, Dalila Laura Vena, Sandesh Neupane, et al.(6D11) (BioLegend, 808008) diluted 1:200 in 1x Cyto-Fast Perm wash solution. Data were acquired on SP6800 spectral analyzer (Sony Biotechnology Inc), and analysis was performed using FlowJo 10 (Tree Star). Plasmids pCMV-VSV-G

(5) Endometrial immune cell profile at the time of frozen embryo transfer as prognostic indicator of live birthFront ImmunolMarch 6, 2026Suset Rodriguez, Laura Padula, Eva Fisher, Bria Slater, Sujad Younis, Carmel Awadallah, Aryana Mohtasham Gharagozloo, Roberta Carlotta Rubino, Mohammed Ibrahim, Michael Paidas, et al.at 2,500 rpm and then fixed with 2% paraformaldehyde. Data were collected on Spectral analyzer SONY SP6800 instrument (Sony Biotechnologies, Inc, San Jose, CA, USA), acquiring at least 100,000 total events.

(6) NRF2 activators and the inhibitor of nuclear export, selinexor, restrict coronaviruses by targeting a network involving ACE2, TMPRSS2, and XPO1 through an NRF2-independent mechanismCommunications BiologyFebruary 20, 2026Fakhar H. Waqas, Leandro Silva da Costa, Francisco J. Zapatero-Belinchón, Madalina E. Carter-Timofte, Lisa Lasswitz, Demi van der Horst, Rebecca Möller, Julia Dahlmann, Ruth Olmer, Robert Geffers, et al.PBS, cells were resuspended with cold PBS for measurement of mitochondrial ROS by flow cytometry (Sony SP6800 ZE Analyzer, phycoerythrin channel). RNA sequencing Quality and integrity of total RNA was controlled

(7) IgG4 anti‐ CD47 : Erythrophagocytosis and potential transfusion complicationsTransfusionFebruary 6, 2026Kayluz Frias Boligan, Melika Loriamini, Mairead B. Holton, Gurleen Sandhu, Beth Binnington, Randall Velliquette, Connie M. Westhoff, Donald R. Branch(Southern Biotech, clone HP6025, Cat. # 9200‐09) for 30 min at 4°C. Fluorescence was acquired in a Sony SP6800 Spectral Analyzer (Sony Biotechnology) and analyzed using FlowJo (BD Biosciences) software. 2.5 Addition of complement Serum

(8) A circadian checkpoint relocates neutrophils to minimize injuryJournal of Experimental MedicineFebruary 2, 2026Alejandra Aroca-Crevillén, Sandra Martín-Salamanca, Lidiane S. Torres, Georgiana Crainiciuc, Jon Sicilia, Eduardo Peñaloza-Martínez, Nicolás Rosillo, Miguel Molina-Moreno, Jose M. Adrover, Andrea Rubio-Ponce, et al.darkness schedule). Flow cytometry and cell sorting Cytometric analyses were performed using a Sony SP6800 Spectral Analyzer (Sony Biotechnology), Canto 3L (BD Biosciences) or FACSymphony (BD Biosciences). Cell sorting experiments

(9) Multimodal profiling of pancreatic cancer reveals a TIMP-1-dominated secretory profile determining pro-tumor immunoinstruction in human cancersCell Reports MedicineJanuary 20, 2026Julian Frädrich, Carmen Mota Reyes, Michel Hendel, Vanessa Brunner, Batu Toledo, Damjan Manevski, Alexander Sommer, Daniel Häußler, Dominik Beck, Daniele Lucarelli, et al.min at RT. Flow cytometry was performed using an SA3800 Spectral Analyzer (Sony Biotechnologies) or SP6800 Spectral Analyzer (Sony Biotechnologies) and data was analyzed using the FlowJo software (v.10.10.0, BD Biosciences).

(10) Microbial-derived immunostimulatory small molecule augments anti-PD-1 therapy in lung cancerCell Reports MedicineJanuary 20, 2026Rachel C. Newsome, Huijia Liu, Bright Agbodzi, Raad Z. Gharaibeh, Liang Zhou, Christian Jobincells were stained with IFNγ PE (Biolegend, clone B27). Flow cytometry was performed on a Sony Spectral SP6800 flow cytometer in the UF ICBR (RRID:SCR_019119, Sony) and analyzed using FlowJo software version 10.6.1.

(11) Cellular and molecular profiling of collagenous gastritis implicates pathogenic CD4+ T cellsiScienceJanuary 16, 2026Michael J. Walsh, Lestat R. Ali, Jiao Shen, Yuko Kinowaki, C. Elizabeth Keleher, Enoch Chung, Komson Wannasai, Maria Ganci, Li Qiang, Mary Thompson, et al.PBS with 2% FBS containing flow cytometry antibodies. Analysis was performed on a Sony Biotechnology SP6800 Spectral Analyzer and analyzed with the Sony Biotechnology SP6800 Software and FlowJo (Tree Star, Ashland, OR). Flow cytometry

(12) Exploring anti-cancer activities of epidermal growth factor-immobilized polymeric nanoparticlesScience and Technology of Advanced MaterialsDecember 31, 2025Shota Yamamoto, Chia-Jung Chang, Masao Kamimura, Jun Nakanishiincubation at room temperature for 15 min, the stained cells were detected using the cell analyzer SP6800 (Sony, Japan). 2.8. Colocalization study of membrane rafts and EGF-PSNPs HeLa cells were seeded onto

(13) Resident alveolar macrophages are highly susceptible to the induction of RIPK1/RIPK3-mediated necroptosis which promote efficient pulmonary immune responseCell Death DisDecember 22, 2025Takumi Adachi, Kazufumi Matsushita, Koubun Yasuda, Masakiyo Nakahira, Mai Imasaka, Michihiko Sugimoto, Zhuohao Yang, Kouji Kobiyama, Tomoya Hayashi, Ken J. Ishii, et al.Gr-1- and PerCP-Cy5.5, and propidium iodide (a viability dye) and then evaluated using a cell analyzer (SP6800; Sony Biotechnology, San Jose, CA). Immunization Ovalbumin (OVA, 10 μg) or recombinant type A influenza virus

(14) Oncostatin M induced by STAT5-activating oncogenes promotes disease progression in hematologic malignanciesSignal Transduct Target TherDecember 11, 2025Michael Rassner, Tony Andreas Müller, Kirstyn Anne Crossley, Geoffroy Andrieux, Sabina Schaberg, Cornelia Endres, Lena Jakob, Teresa Poggio, Natalie Köhler, Julia Kolter, et al.cell sorting Flow cytometry analysis was performed on a BD LSR Fortessa™, Beckman CyAn™ ADP, or Sony SP6800 Spectral Analyzer. The cells were sorted on a BD FACSAria III. The antibodies used to stain the following cell surface

(15) Peptide-MHC-targeted retroviruses enable in vivo expansion and gene delivery to tumor-specific T cellsScience advancesNovember 7, 2025Xu EJK, Smith BE, Conce Alberto WD, Walsh MJ, Lim B, Hoffman MT, Qiang L, Barreiro A, Finburgh EN, Dong J, et al.were then washed once with FACS buffer before analysis on a Cytoflex S (Beckman) or Sony Biotechnology SP6800 Spectral Analyzer. For experiments where absolute cell counts were calculated, CountBright Absolute Counting Beads (Thermo

(16) Platelet Jak2 deficiency accelerates atherosclerosis with increased inflammatory responseJournal of Biological ChemistryOctober 1, 2025Daeun Kim, Yu Zhe Li, Jiaqi Yang, Brian Lin, Meng Yun Wang, Jessie Jia Yu Yu Liu, Jia Qi Adam Adam Bai, Gedaliah Farber, Faisal Abdul Abdul Razzaq, Evan Pollock-Tahiri, et al.Samples were then diluted with 500 μl of Tyrode’s buffer lacking calcium and analyzed using a Sony SP6800 spectral cytometer. Platelet activation was quantified as the median fluorescence intensity of JON/A

(17) Dynamic expression of candidalysin facilitates oral colonization of Candida albicans in miceNature MicrobiologySeptember 25, 2025Ricardo Fróis-Martins, Julia Lagler, Tim B. Schille, Osama Elshafee, Kontxi Martinez de San Vicente, Sarah Mertens, Michelle Stokmaier, Iman Kilb, Natacha Sertour, Sophie Bachellier-Bassi, et al.Biolegend 123109, used at 1/100 dilution). Cells were fixed in 4.2% formaldehyde before acquisition on a SP6800 Spectral Analyzer (Sony). Data analysis was performed using FlowJo software v10 (FlowJo LLC). The gating of the flow cytometric

(18) Kupffer cells are essential for platelet-mediated thrombopoietin generation in the liverProceedings of the National Academy of Sciences of the United States of AmericaSeptember 23, 2025Karakas D, Li J, Ma W, Wu XG, Khoury CJ, Masoud M, Hoard B, Liu YJ, Zhu G, Chen J, et al.with 0.5 μg/mL anti-CD68 (FA-11) (Biolegend) for 1 h at room temperature. Cells were analyzed via Sony SP6800 flow cytometer. Hepatic Platelet Localization Confocal Microscopy. Mice were intraperitoneally injected

(19) Membrane receptors cluster phosphatidylserine to activate LC3-associated phagocytosisNature Cell BiologySeptember 22, 2025Emilio Boada-Romero, Clifford S. Guy, Gustavo Palacios, Luigi Mari, Suresh Poudel, Zhenrui Li, Piyush Sharma, Douglas R. Greenafter the probe, primary and secondary antibody incubations. Cell fluorescence was acquired using a SP6800 Sony Spectral Analyzer, and compensated values were analysed using the FlowJo v10 software. When cells

(20) Discovery of BMS-986408, a First-In-Class Dual DGKα and DGKζ Inhibitor that Unleashes PD-1 Checkpoint and CAR T-cell ImmunotherapiesCancer immunology researchSeptember 2, 2025Wichroski M, Liu SQ, Zasadil LM, Benci JL, Gedeon PC, Condon KJ, Joshi S, Posy S, Carlson P, Maier A, et al.then stained with antibodies listed in Supplementary Table S2, and samples were recorded using a Sony SP6800 spectral flow cytometer. Human peripheral blood mononuclear cell assay and synergy analysis Human

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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer from Sony Corporation

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SP6800 Spectral Analyzer from Sony Corporation

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