SP6800 Spectral Analyzer from Sony Corporation

Product Specs
ItemSP6800 Spectral Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberSP6800
DetectionFSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
Lasers2 or 3 lasers
Excitation405nm (60mW), 488nm (40mW), 638nm (60mW)
Parameters64 fluorescence channels
CapacityTubes (5 mL)
Data Throughput10,000 events/sec; 20,000 events/sec (max)
Dimensions(WxDxH) 60.0 x 63.5 x 71.3 cm

(81) Blockade of innate inflammatory cytokines TNFα, IL-1β, or IL-6 overcomes virotherapy-induced cancer equilibrium to promote tumor regressionImmunotherapy AdvancesJuly 3, 2023Michael J Walsh, Lestat R Ali, Patrick Lenehan, Courtney T Kureshi, Rakeeb Kureshi, Michael Dougan, David M Knipe, Stephanie K DouganPBS and fixed with 1% formalin in PBS before analysis, which was performed on a Sony Biotechnology SP6800 Spectral Analyzer and analyzed with the Sony Biotechnology SP6800 Software and FlowJo (Tree Star, Ashland, OR). Flow cytometry

(82) NRF2 activators inhibit influenza A virus replication by interfering with nucleo-cytoplasmic export of viral RNPs in an NRF2-independent mannerPLoS pathogensJuly 1, 2023Waqas FH, Shehata M, Elgaher WAM, Lacour A, Kurmasheva N, Begnini F, Kiib AE, Dahlmann J, Chen C, Pavlou A, et al.PBS, cells were resuspended with cold PBS for measurement of mitochondrial ROS by flow cytometry (Sony SP6800 ZE Analyzer, phycoerythrin channel). Infection of iPSC-derived EC Cells were grown in fibronectin

(83) Liposomes loaded with vitamin D3 induce regulatory circuits in human dendritic cellsFrontiers in ImmunologyJune 9, 2023Noémi Anna Nagy, Fernando Lozano Vigario, Rinske Sparrius, Toni M. M. van Capel, Ronald van Ree, Sander W. Tas, I. Jolanda M. de Vries, Teunis B. H. Geijtenbeek, Bram Slütter, Esther C. de Jong, et al.well as anti-PD-L1 (CD274)-PE-Cy7 (all eBioscience, Thermo Fisher) and 10.000 DCs (3) acquired on the SP6800 Spectral Analyzer (Sony). Isolation of naïve and memory CD4+ T cells The total CD4+ T-cell population was isolated from

(84) Combined phospholipids adjuvant augments anti-tumor immune responses through activated tumor-associated dendritic cellsNeoplasiaMay 1, 2023Yifang Shui, Xin Hu, Hiroshi Hirano, Hirotake Tsukamoto, Wen-Zhi Guo, Kenichiro Hasumi, Fumihiro Ijima, Masayuki Fujino, Xiao-Kang Lilabeled mAbs were shown in Table 1. All samples were acquired using a SONY Spectral Cell Analyzer LE-SP6800 (SONY, Tokyo, Japan) or BD FACSymphonyTM (BD Biosciences), and analyzed using the FlowJo software program

(85) Fluorescent Protein Production, Purification, and Coupling to MicrospheresCurrent ProtocolsApril 18, 2023Marija Dramicanin, Kevin Lim, Simon MonardInformation The first spectral cytometer became commercially available in 2014, when Sony released the SP6800™ instrument. The instrument used a single 32‐channel PMT as the detector. In 2017, Cytek Bio released

(86) PD-1 blockade and CDK4/6 inhibition augment nonoverlapping features of T cell activation in cancerThe Journal of experimental medicineApril 3, 2023Ali LR, Garrido-Castro AC, Lenehan PJ, Bollenrucher N, Stump CT, Dougan M, Goel S, Shapiro GI, Tolaney SM, Dougan SK.heat-inactivated FBS in PBS, and staining was done at 4°C for 20 min. Samples were then analyzed on a Sony SP6800 Spectral Analyzer. Gating and analysis of flow cytometry data was done in FlowJo 10.8.1. Samples that yielded fewer than

(87) Variability of fluorescence intensity distribution measured by flow cytometry is influenced by cell size and cell cycle progressionScientific reportsMarch 25, 2023Fedr R, Kahounová Z, Remšík J, Reiterová M, Kalina T, Souček K.experiments: FACSVerse (BD Biosciences), Attune (1st generation, TFS), FACSCalibur (BD Biosciences), and SP6800 spectral analyzer (SONY). The advantage of including spectral analyzer on the top of conventional flow cytometers is that

(88) Aberrant JAK-STAT signaling-mediated chromatin remodeling impairs the sensitivity of NK/T-cell lymphoma to chidamideClinical EpigeneticsFebruary 6, 2023Jinghong Chen, Zhixiang Zuo, Yan Gao, Xiaosai Yao, Peiyong Guan, Yali Wang, Zhimei Li, Zhilong Liu, Jing Han Hong, Peng Deng, et al.propidium iodide (PI, Thermo Fisher Scientific) for 40 min in the dark and analyzed by flow cytometry (SP6800, Sony). Cell apoptosis assay 2 × 105 cells were treated with indicated drugs for 72 h. Cells were

(89) Who Knew? Dopamine Transporter Activity Is Critical in Innate and Adaptive Immune ResponsesCellsJanuary 10, 2023Gopinath A, Mackie PM, Phan LT, Mirabel R, Smith AR, Miller E, Franks S, Syed O, Riaz T, Law BK, et al.Number,Purpose Centrifuge,Sorvall,ST8,PBMC isolation Cytometer,BD,Canto II,FC Spectral Analyzer,Sony,SP6800,FC Spectral Analyzer,Cytek,Aurora 5 L,FC Flow cytometer,Beckman,Cytoflex LX,"FC, 13-plex ELISA" Microcentrifuge,Fisher,59A,FC Equipment.

(90) Intestinal colonization regulates systemic anti-commensal immune sensitivity and hyperreactivityFrontiers in immunologyJanuary 1, 2023Burkhard R, Koegler M, Brown K, Wilson K, Mager LF, Zucoloto AZ, Thomson C, Hebbandi Nanjundappa R, Skalosky I, Ahmadi S, et al.(clone RM4-5, BD Biosciences) and CD8a-AF700 (clone 53-6.7, BD Biosciences). Data was acquired on a Sony SP6800 flow cytometer, and analyzed using FlowJo software (TreeStar). 2.6 LCMV infection LCMV-Armstrong was

(91) Lower Metabolic Potential and Impaired Metabolic Flexibility in Human Lymph Node Stromal Cells from Patients with Rheumatoid ArthritisCellsDecember 20, 2022Tineke A. de Jong, Johanna F. Semmelink, Simone W. Denis, Janne W. Bolt, Mario Maas, Marleen G. H. van de Sande, Riekelt H. L. Houtkooper, Lisa G. M. van Baarsencontaining 0.5% BSA and 0.01% NaN3 (Sigma Aldrich, St. Louis, MO, USA)) and measured on a Spectral Analyzer SP6800 (Sony Biotechnology, Weybridge, UK). Data were analyzed using FlowJo v10.8.1 (TreeStar Inc., Ashland,

(92) Non-Modified CpG Oligodeoxynucleotide Forming Guanine-Quadruplex Structure Complexes with ε-Poly-L-Lysine Induce Antibody Production as Vaccine AdjuvantsBiomoleculesDecember 13, 2022Dandan Zhao, Anh Thi Tram Tu, Miwako Shobo, Nguyen Bui Thao Le, Chiaki Yoshikawa, Kazuhisa Sugai, Yoji Hakamata, Tomohiko Yamazakiresuspended in 500 μL PBS. The mean fluorescence intensity (MFI) was measured by a flow cytometer (SP6800, Sony, Tokyo, Japan). Confocal microscopy was used to visualize the cellular uptake. RAW264 cells (1.2

(93) The human pathobiont Malassezia furfur secreted protease Mfsap1 regulates cell dispersal and exacerbates skin inflammationProceedings of the National Academy of SciencesDecember 6, 2022Joleen P. Z. Goh, Fiorella Ruchti, Si En Poh, Winston L. C. Koh, Kiat Yi Tan, Yan Ting Lim, Steven T. G. Thng, Radoslaw M. Sobota, Shawn S. Hoon, Chenxi Liu, et al.dead cells. All staining steps were carried out on ice. Cells were acquired on a Spectral Analyzer SP6800 (Sony), and the data were analyzed with FlowJo software (FlowJo LLC). The gating of the flow cytometric

(94) Near-infrared photoimmunotherapy induced tumor cell death enhances tumor dendritic cell migrationCancer Immunology, ImmunotherapyDecember 1, 2022Taiki Moriya, Mayuko Hashimoto, Hina Matsushita, Shion Masuyama, Rina Yoshida, Ryuhei Okada, Aki Furusawa, Daiki Fujimura, Hiroaki Wakiyama, Takuya Kato, et al.fluorochrome-conjugated mAbs for 15 min at 4 °C, and then, cells were analyzed by flow cytometry analysis with SP6800 (SONY). Flow cytometry data were analyzed using FlowJo software (Tree Star). The number of cells in

(95) Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterolsNature CommunicationsDecember 1, 2022Björn D. M. Bean et al.500 µl at 30 °C with shaking at 1000 rpm. 10,000 singlet cells of each sample were analyzed using a SP6800 Spectral Analyzer (Sony). Microscopy Log phase yeast grown in synthetic selective media were mounted on slides and imaged

(96) Cellular and antibody response in GMZ2-vaccinated Gabonese volunteers in a controlled human malaria infection trialMalaria JournalDecember 1, 2022Odilon Nouatin, Javier Ibáñez, Rolf Fendel, Ulysse A. Ngoa, Freia-Raphaella Lorenz, Jean-Claude Dejon-Agobé, Jean Ronald Edoa, Judith Flügge, Sina Brückner, Meral Esen, et al.recommendations (BD Biosciences). Cell acquisition was performed using a Spectral Cell Analyzer cytometer SP6800 (Sony Biotechnology) 15-color cytometer, with 100,000 cells per tube as the total number of cells acquired.

(97) Secreted heat shock protein gp96-Ig and OX40L-Fc combination vaccine enhances SARS-CoV-2 Spike (S) protein-specific B and T cell immune responsesVaccine: XDecember 1, 2022Padula L, Fisher E, Rivas K, Podack K, Frasca D, Kupritz J, Seavey MM, Jayaraman P, Dixon E, Jasuja R, et al.kit was used according to manufacturer instructions. Data were collected on Spectral analyzer SONY SP6800 instrument (Sony Biotechnologies, Inc, San Jose, CA, USA). Analysis was performed using FlowJo™ software

(98) Doxorubicin-Induced Platelet Activation and Clearance Relieved by Salvianolic Acid Compound: Novel Mechanism and Potential Therapy for Chemotherapy-Associated Thrombosis and ThrombocytopeniaPharmaceuticals (Basel)November 22, 2022Wenjing Ma, Zackary Rousseau, Sladjana Slavkovic, Chuanbin Shen, George M. Yousef, Heyu NiPBS-EDTA or washed by centrifugation after 20–30 min of incubation in the dark before analyzing on SP6800 Spectral Analyzer (Sony Biotechnology, Inc, San Jose, CA, USA). Spectral unmixing was ensured to separate individual fluorochrome

(99) Estrogen-related receptor alpha drives mitochondrial biogenesis and resistance to neoadjuvant chemoradiation in esophageal cancerCell reports. MedicineNovember 15, 2022Dings MPG, van der Zalm AP, Bootsma S, van Maanen TFJ, Waasdorp C, van den Ende T, Liu D, Bailey P, Koster J, Zwijnenburg DA, et al.lentiviral knockdown and overexpression). After 72 h, the cells were analyzed on the spectral FACS (Sony SP6800, Tokyo, Japan). ESRRA ChIP qPCR Following exposure to chemoradiation, cells were processed for pull-down

(100) Unlocking autofluorescence in the era of full spectrum analysis: Implications for immunophenotype discovery projectsCytometry. Part A : the journal of the International Society for Analytical CytologyNovember 1, 2022Jameson VJ, Luke T, Yan Y, Hind A, Evrard M, Man K, Mackay LK, Kallies A, Villadangos JA, McWilliam HEG, et al.of optical and electronics design and the availability of spectral cytometers (Cytek Aurora and Sony SP6800) (13, 30, 31), the development of strategies to capture and extract AF signatures from polychromatic

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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer from Sony Corporation

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SP6800 Spectral Analyzer from Sony Corporation

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