SP6800 Spectral Analyzer from Sony Corporation

Product Specs
ItemSP6800 Spectral Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberSP6800
DetectionFSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
Lasers2 or 3 lasers
Excitation405nm (60mW), 488nm (40mW), 638nm (60mW)
Parameters64 fluorescence channels
CapacityTubes (5 mL)
Data Throughput10,000 events/sec; 20,000 events/sec (max)
Dimensions(WxDxH) 60.0 x 63.5 x 71.3 cm

(101) Autofluorescence identifies highly phagocytic tissue-resident macrophages in mouse and human skin and cutaneous squamous cell carcinomaFrontiers in ImmunologyOctober 17, 2022Pierre Bourdely, Luciana Petti, Sokchea Khou, Aida Meghraoui-Kheddar, Roxane Elaldi, Julie Cazareth, Noushine Mossadegh-Keller, Julien Boyer, Michael H. Sieweke, Gilles Poissonnet, et al.were then fixed and permeabilized with Cytofix/cytoperm kit (BD Biosciences). Data were acquired on a SP6800 spectral cytometer (SONY Biotechnology) (12) and analyzed with FlowJo V10 software (Treestar) using

(102) (NZW × BXSB) F1 male mice: An unusual, severe and fatal mouse model of lupus erythematosusFrontiers in ImmunologySeptember 23, 2022Ruqayyah J. Almizraq, Kayluz Frias Boligan, Melika Loriamini, Colin McKerlie, Donald R. Branchan Alexa Fluor® 488-AffiniPure Goat Anti-Mouse IgG + IgM (H+L) antibody. Samples were acquired in an SP6800 Spectral Analyzer (Sony Biotechnology) and analyzed with FlowJo V10 (BD Biosciences). Staining with only the secondary

(103) Targeting Serotonin With Common Antidepressants Induces Rapid Recovery From CytopeniaStem Cells Translational MedicineSeptember 21, 2022Guillemette Fouquet, Julien Rossignol, Nicolas Garcelon, Olivier Hermine, Francine Côté, Tereza Comanand BD Biosciences. Cells were analyzed on a FACSCanto II (BD Biosciences, San Jose, CA, USA) or an SP6800 Spectral Analyzer (Sony Biotechnology, San Jose, CA, USA) using FlowJo v. X.0.7 (Tree Star, Ashland, OR, USA). Supplementary

(104) Heme-stress activated NRF2 skews fate trajectories of bone marrow cells from dendritic cells towards red pulp-like macrophages in hemolytic anemiaCell death and differentiationAugust 1, 2022Vallelian F, Buzzi RM, Pfefferlé M, Yalamanoglu A, Dubach IL, Wassmer A, Gentinetta T, Hansen K, Humar R, Schulthess N, et al.(Sigma-aldrich). Multiparameter analysis was performed with an LSRFortessa analyzer (BD Biosciences), a SP6800 Spectral Analyzer (Sony) or an Aurora 5 L spectral flow cytometer (Cytek). The data were analyzed using FlowJo software

(105) Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolusPLOS PathogensJuly 11, 2022Sereina O. Sutter, Anouk Lkharrazi, Elisabeth M. Schraner, Kevin Michaelsen, Anita Felicitas Meier, Jennifer Marx, Bernd Vogt, Hildegard Büning, Cornel Fraefelincubation, the cells were washed twice with PBS and then resuspended in 200 μl PBS prior to analysis (SONY SP6800 Spectral Analyzer). For confocal laser scanning microscopy, the coverslips were embedded in ProLong Anti-Fade mountant

(106) Vitamin D3 Priming of Dendritic Cells Shifts Human Neutrophil-Dependent Th17 Cell Development to Regulatory T CellsFrontiers in ImmunologyJuly 7, 2022Florianne M. J. Hafkamp, Esther W. M. Taanman-Kueter, Toni M. M. van Capel, Tom Groot Kormelink, Esther C. de Jongh of stimulation with αCD3 and αCD28. A total of 20,000 cells were acquired in the live gate on the SP6800 Spectral Analyzer (Sony, San Jose, CA, USA). The following antibodies were used: αCD3-FITC, αCD4-APC, αCD45RA-FITC, αIFNγ-FITC,

(107) IDO1 plays a tumor-promoting role via MDM2-mediated suppression of the p53 pathway in diffuse large B-cell lymphomaCell death & diseaseJune 27, 2022Sun C, Li M, Zhang L, Sun F, Chen H, Xu Y, Lan Y, Zhang L, Lu S, Zhu J, et al.µg/ml) and 400 µl PBS were added to the cells, and the stained cells were analyzed by flow cytometry (SP6800, Sony, Japan) within 1 hour. As previously described [27], DLBCL cells were fixed with precooled 70%

(108) Immunorthodontics: Role of HIF-1α in the Regulation of (Peptidoglycan-Induced) PD-L1 Expression in Cementoblasts under Compressive ForceInternational Journal of Molecular SciencesJune 23, 2022Jiawen Yong, Sabine Gröger, Joerg Meyle, Sabine RufBerlin, Germany). 2.9. Quantification of Necroptotic Cell Populations The data was obtained using the SP6800 Spectral Analyzer software version 2.0.2 (Sony Corporation, Tykyo, Japan) for analysis. Data were analyzed using the FlowJo

(109) DAT and TH expression marks human Parkinson's disease in peripheral immune cellsNPJ Parkinson's diseaseJune 7, 2022Gopinath A, Mackie P, Hashimi B, Buchanan AM, Smith AR, Bouchard R, Shaw G, Badov M, Saadatpour L, Gittis A, et al.number,Purpose Centrifuge,Sorvall,ST8,PBMC isolation Cytometer,BD,Canto II,FC Spectral Analyzer,Sony,SP6800,FC Microcentrifuge,Fisher,59 A,FC Dual syringe infusion pump,Harvard Apparatus,,Surgery Stereotax,Kopf,Model

(110) Candida albicans commensalism in the oral mucosa is favoured by limited virulence and metabolic adaptationPLoS pathogensApril 1, 2022Lemberg C, Martinez de San Vicente K, Fróis-Martins R, Altmeier S, Tran VDT, Mertens S, Amorim-Vaz S, Rai LS, d'Enfert C, Pagni M, et al.antibodies were from BioLegend. Stained cells were analysed on a FACS Gallios (Beckman Coulter), or a SP6800 Spectral Analyzer (Sony). The data were analysed with FlowJo software (FlowJo LLC). The gating of the flow cytometric

(111) Decreased IL-10 accelerates B-cell leukemia/lymphoma in a mouse model of pediatric lymphoid leukemiaBlood AdvancesFebruary 1, 2022Briana A. Fitch, Mi Zhou, Jamilla Situ, Sangeetha Surianarayanan, Melissa Q. Reeves, Michelle L. Hermiston, Joseph L. Wiemels, Scott C. Koganusing the BD Cytofix/Cytoperm Kit per the manufacturer’s instructions. Data were acquired using a Sony SP6800 Spectral Analyzer (Sony Biotechnology) and analyzed using FlowJo software. Peripheral blood analyses For peripheral

(112) Glioma-derived CCL2 and CCL7 mediate migration of immune suppressive CCR2(+)/CX3CR1(+) M-MDSCs into the tumor microenvironment in a redundant mannerFrontiers in immunologyJanuary 1, 2022Takacs GP, Kreiger CJ, Luo D, Tian G, Garcia JS, Deleyrolle LP, Mitchell DA, Harrison JK.stained with a viability dye. Stained samples were analyzed using single-color compensation on a Sony SP6800 spectral analyzer or Beckman Coulter CytoFLEX LX 96-well plate system and quantified using FlowJo V10.8.1 (BD Biosciences). Cell culture

(113) Inhibition of the PLK1-Coupled Cell Cycle Machinery Overcomes Resistance to Oxaliplatin in Colorectal CancerAdvanced science (Weinheim, Baden-Wurttemberg, Germany)December 1, 2021Yu Z, Deng P, Chen Y, Liu S, Chen J, Yang Z, Chen J, Fan X, Wang P, Cai Z, et al.(Biolegend, 641006) and propidium iodide. The stained cells were analyzed for DNA content using an SP6800 flow cytometry (Sony Biotechnology, Tokyo, Japan). Plasmid Constructs and Inducible Cell lines Full‐length

(114) In Vivo Bioluminescence Imaging of HBV Replicating Hepatocytes Allows for the Monitoring of Anti-Viral ImmunityVirusesNovember 13, 2021Katrin Manske, Annika Schneider, Chunkyu Ko, Percy A. Knolle, Katja Steiger, Ulrike Protzer, Dirk WohlleberStaining Buffer Set (BD Biosciences, Franklin Lakes, NJ, USA). Flow cytometric analysis was performed with SP6800 Spectral Analyzer (Sony Biotechnology, Bothell, WA, USA). The data were analyzed by FlowJo software V10.1 (TreeStar Inc.,

(115) Glomerular endothelial cell senescence drives age-related kidney disease through PAI-1EMBO molecular medicineNovember 8, 2021Cohen C, Le Goff O, Soysouvanh F, Vasseur F, Tanou M, Nguyen C, Amrouche L, Le Guen J, Saltel-Fulero O, Meunier T, et al.Annexin‐V‐FITC. Quantification of Annexin‐V‐FITC‐positive cells was performed by FACS, using a Sony SP6800. Beta galactosidase staining (SA‐βGal) Five‐micrometer frozen kidney sections or cells on coverslips

(116) Radiation combines with immune checkpoint blockade to enhance T cell priming in a murine model of poorly immunogenic pancreatic cancerOpen biologyNovember 1, 2021Stump CT, Roehle K, Manjarrez Orduno N, Dougan SK.4°C, washed with PBS and resuspended in 1% formalin before analysis on a Sony spectral flow cytometer (SP6800). Flow cytometry antibodies were purchased from BioLegend. Myeloid panel: CD11c FITC (no. 117305), CX3CR1

(117) Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic TransmissionmBioOctober 26, 2021Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, et al.M5/114.15.2), and PE/Cy7-CX3CR1 (clone SA011F11) (purchased from BioLegend). Cells were acquired using an SP6800 spectral cell analyzer (Sony Biotechnology, San Jose, CA, USA), and obtained data were analyzed using

(118) Hypoxia-inducible factor 1-alpha acts as a bridge factor for crosstalk between ERK1/2 and caspases in hypoxia-induced apoptosis of cementoblastsJournal of cellular and molecular medicineOctober 1, 2021Yong J, von Bremen J, Groeger S, Ruiz-Heiland G, Ruf S.cells were kept on ice and analysed for apoptosis and necrosis using a FACS Vantage Flow Cytometer (SP6800 Spectral Analyzer, Sony Biotechnology, Berlin, Germany) within 1 hr. The percentages of the different cell populations

(119) Lymph Node Stromal Cell–Intrinsic MHC Class II Expression Promotes MHC Class I–Restricted CD8 T Cell Lineage Conversion to Regulatory CD4 T CellsThe Journal of ImmunologySeptember 15, 2021Amanda M. Honan, Emily N. Vazquez, Zhibin Chenexpression by single cells. Samples were analyzed with a Cytoflex from Beckman Coulter (Brea, CA) or Sony SP6800 from Sony Biotechnology (San Jose, CA). Cell purification was done with FACS Aria sorter (BD Bioscience). Lymph node

(120) Monobac System–A Single Baculovirus for the Production of rAAVMicroorganismsAugust 24, 2021Lionel Galibert et al.process was monitored by spectral flow cytometry. The cell samples were analyzed on a spectral analyzer SP6800 (Sony Biotechnology, Weybridge, Surrey, UK). The SP6800 was equipped with three lasers: 405 nm, 488

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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer from Sony Corporation

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SP6800 Spectral Analyzer from Sony Corporation

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