SP6800 Spectral Analyzer from Sony Corporation

Product Specs
ItemSP6800 Spectral Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberSP6800
DetectionFSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
Lasers2 or 3 lasers
Excitation405nm (60mW), 488nm (40mW), 638nm (60mW)
Parameters64 fluorescence channels
CapacityTubes (5 mL)
Data Throughput10,000 events/sec; 20,000 events/sec (max)
Dimensions(WxDxH) 60.0 x 63.5 x 71.3 cm

(41) Phase I study of ribociclib (CDK4/6 inhibitor) with spartalizumab (PD-1 inhibitor) with and without fulvestrant in metastatic hormone receptor-positive breast cancer or advanced ovarian cancerJ Immunother CancerFebruary 25, 2025Ana C Garrido-Castro, Noah Graham, Lestat R Ali, Christina Herold, Jennifer Desrosiers, Khanh Do, Heather Parsons, Tianyu Li, Shom Goel, Molly DiLullo, et al.incubation, the cells were washed and fixed with 1% formalin, then analyzed by flow cytometry using a Sony SP6800 Spectral Analyzer. Gating for cell populations of interest was performed with FlowJo software. Changes in cell populations

(42) Super-enhancer–driven EFNA1 fuels tumor progression in cervical cancer via the FOSL2-Src/AKT/STAT3 axisJ Clin InvestFebruary 18, 2025Shu-Qiang Liu, Xi-Xi Cheng, Shuai He, Tao Xia, Yi-Qi Li, Wan Peng, Ya-Qing Zhou, Zi-Hao Xu, Mi-Si He, Yang Liu, et al.Fisher Scientific) for 30 minutes at room temperature. Cell cycle distribution was assessed using an SP6800 Spectral Analyzer (Sony) and analyzed using FlowJo v10 software. Cell apoptosis was evaluated through flow cytometry using

(43) Immunostimulatory Effects of Guanine-Quadruplex Topologies as Scaffolds for CpG OligodeoxynucleotidesBiomoleculesJanuary 10, 2025Pathak S, Le NBT, Oyama T, Odahara Y, Momotake A, Ikebukuro K, Kataoka-Hamai C, Yoshikawa C, Kawakami K, Kaizuka Y, et al.PBS, and the mean fluorescence intensity (MFI) from Cy5 was quantified using a spectral cell analyzer (SP6800, Sony, Tokyo, Japan). 2.11. Confocal Microscopy The localization of G4 CpG ODNs was examined via confocal microscopy

(44) SMAC mimetics induce human macrophages to phagocytose live cancer cellsImmunother AdvJanuary 1, 2025Liu SY, Hulsman MPM, Leyendecker P, Chang E, Donovan KA, Strobel F, Dougan J, Fischer ES, Dougan M, Dougan SK, et al.tubes to fix the samples. Tubes were stored at 4°C and protected from light until time of analysis on a SP6800 Spectral Analyzer (Sony). Immunoblotting Differentiated macrophages were reseeded in 6-well tissue culture-treated plates

(45) Characterization of naphthoquinones as inhibitors of glutathione reductase and inducers of intracellular oxidative stressRedox ReportDecember 31, 2024Xiaowan Chen, Yan Ma, Ziming Yang, Dingjie Shen, Xia Li, Maowei Ni, Xiaoling Xu, Wei ChenReagent for 30 min. The intracellular green fluorescence intensity was analyzed by flow cytometry (SP6800, SONY). Data analysis was performed using FlowJo software. 2.10. In vivo study ICR mice (5 weeks,

(46) Bone morphogenetic protein 4 induces hematopoietic stem cell development from murine hemogenic endothelial cells in cultureStem cell reportsDecember 10, 2024Tsuruda M, Morino-Koga S, Zhao X, Usuki S, Yasunaga KI, Yokomizo T, Nishinakamura R, Suda T, Ogawa M.TER119, CD4, CD8a, CD11b, and Gr-1. After staining dead cells with PI, cells were analyzed using an SP6800 spectral cell analyzer (SONY). In some experiments, Lin− KIT+ Sca-1+ KSL cells were sorted using the

(47) Targeted immune cell therapy for hepatocellular carcinoma using expanded liver mononuclear cell-derived natural killer cellsNeoplasiaDecember 1, 2024Xin Hu, Yifang Shui, Seiichi Shimizu, Seisuke Sakamoto, Mureo Kasahara, Seiji Okada, Wen-Zhi Guo, Masayuki Fujino, Xiao-Kang Liusing either a BD FACSymphony™ (BD Biosciences, San Diego, CA) or a SONY Spectral Cell Analyzer LE-SP6800 (Sony, Tokyo, Japan). Data analyses were conducted using the FlowJo software program (version 10.5.0;

(48) Sublingual immune cell clusters and dendritic cell distribution in the oral cavityJCI InsightNovember 8, 2024Yutaka Kusumoto, Mizuki Ueda, Mayuko Hashimoto, Haruka Takeuchi, Naoko Okada, Junya Yamamoto, Akiko Nishii, Atsuki Fujino, Akiho Kurahashi, Momoka Satoh, et al.stained with fluorochrome-conjugated mAbs shown in Supplemental Table 2 and analyzed by flow cytometry (SP6800, SONY). KikGR+ clusters in CD11c-KikGR mice were photolabeled and analyzed in the same way as above.

(49) SUV39H1 epigenetically modulates the MCPIP1-AURKA signaling axis to enhance neuroblastoma tumorigenesisOncogeneNovember 4, 2024Mengzhen Li, Feifei Sun, Juan Wang, Suying Lu, Yi Que, Mengjia Song, Huimou Chen, Xiangyu Xiong, Weiji Xie, Jia Zhu, et al.the dark, then 10 μL of 7-AAD was added into each tube, immediately analyzed with the flow cytometry (SP6800, Sony, Japan) within 1 h. Cell cycle analysis The cell cycle phases of NB cells were analyzed by flow cytometry

(50) Mechanism of Drug Resistance to First‐Line Chemotherapeutics Mediated by TXNDC17 in NeuroblastomasCancer Rep (Hoboken)October 16, 2024Chenggong Zeng, Zhuoran Li, Zhiqing Wei, Tingting Chen, Juan Wang, Junting Huang, Feifei Sun, Jia Zhu, Suying Lu, Zijun ZhenFXP018‐100), and single‐dye tubes were prepared. The samples were analyzed by spectral flow cytometer SP6800 (Sony Biotechnology, San Jose, CA, USA). 2.6 Western Blot Proteins were extracted from tumor cells

(51) Obesity intensifies sex-specific interferon signaling to selectively worsen central nervous system autoimmunity in femalesCell MetabolismOctober 1, 2024Brendan Cordeiro, Jeeyoon Jennifer Ahn, Saurabh Gawde, Carmen Ucciferri, Nuria Alvarez-Sanchez, Xavier S. Revelo, Natalie Stickle, Kaylea Massey, David G. Brooks, Joel M. Guthridge, et al.in 1 x FACS buffer for acquisition. Cells were acquired either on an LSR II (BD Biosciences) or Sony SP6800 and were analyzed using FlowJo (TreeStar). OneComp eBeads Compensation Beads (Invitrogen) were used

(52) Human IL-22 receptor-targeted small protein antagonist suppress murine DSS-induced colitisCell Communication and SignalingOctober 1, 2024Milan Kuchař, Kristýna Sloupenská, Leona Rašková Kafková, Yaroslava Groza, Jozef Škarda, Petr Kosztyu, Marie Hlavničková, Joanna M. Mierzwicka, Radim Osička, Hana Petroková, et al.plate was incubated for 30 min at RT. After washing, beads were analyzed using flow cytometer SONY SP6800 (Sony, Tokio, Japan). Histochemistry Formalin-fixed, paraffin embedded tissues were cut, stained with

(53) CD226 implicated in Akt-dependent apoptosis of CD4+ T cell contributes to asthmatic pathogenesisCell Death & DiseaseSeptember 30, 2024Yuan Zhang, Yang Xie, Xuexin Zhang, Chujun Duan, Jingchang Ma, Yuling Wang, Yilin Wu, Niqi Shan, Kun Cheng, Ran Zhuang, et al.(Thermo Fisher Scientific) according to the manufacturer’s instructions. Data were acquired using a Sony SP6800 Spectral Analyzer (Sony Biotechnology, UK) and the NovoExpress software. Apoptosis assay Cellular apoptosis was detected

(54) CSF1R Ligands Expressed by Murine Gliomas Promote M-MDSCs to Suppress CD8(+) T Cells in a NOS-Dependent MannerCancersSeptember 1, 2024Takacs GP, Garcia JS, Hodges CA, Kreiger CJ, Sherman A, Harrison JK.centrifuged twice (500× g, 5 min, RT). All samples were analyzed by single-color compensation on a Sony SP6800 spectral analyzer. Data were quantified using FlowJo V10.8.1 (BD Biosciences, Franklin Lakes, NJ, USA). Flow gating strategy

(55) Single-cell profiling of surface glycosphingolipids opens a new dimension for deconvolution of breast cancer intratumoral heterogeneity and phenotypic plasticityJournal of Lipid ResearchSeptember 1, 2024Jiřina Procházková, Radek Fedr, Barbora Hradilová, Barbora Kvokačková, Josef Slavík, Ondrej Kováč, Miroslav Machala, Pavel Fabian, Jiří Navrátil, Simona Kráčalíková, et al.cells were resuspended in 200 μl of staining buffer and analyzed using Spectral Cell Analyzer SONY SP6800 (Sony Biotechnology). Panels with antibodies and isotype controls are listed in supplemental Table S3.

(56) 19Fluorine‐MRI Based Longitudinal Immuno‐Microenvironment‐Monitoring for Pancreatic CancerJournal of Magnetic Resonance ImagingAugust 27, 2024Wilfried Reichardt, Tabea Gewalt, Philipp Hafner, Steffen J. Keller, Xun Chen, Asma Alrawashdeh, Yayu Li, Solène Besson, Stefan Fichtner‐Feigl, Dominik von Elverfeldt, et al.Fix/Perm wash (BD) at room temperature. Flow cytometry was performed on the Sony Spectral Analyzer SP6800 (Sony Biotechnology, San Jose, CA, USA), and analyzed using FlowJo software (BD Biosciences, Ashland,

(57) Pre-existing cell subpopulations in primary prostate cancer tumors display surface fingerprints of docetaxel-resistant cellsCell Oncol (Dordr)August 20, 2024Stanislav Drápela, Barbora Kvokačková, Eva Slabáková, Anna Kotrbová, Kristína Gömöryová, Radek Fedr, Daniela Kurfürstová, Martin Eliáš, Vladimír Študent jr, Frederika Lenčéšová, et al.leukocytes, endothelial-like cells, and stromal cells [22, 23]. Samples were analyzed using a SONY SP6800 Spectral Cell Analyzer (SONY, Japan). Acquired FCS files were exported and analyzed using FlowJo software

(58) NADPH oxidase-dependent free radical generation and protein adduct formation in neutrophils†RSC AdvancesAugust 5, 2024Renuka Ramalingam Manoharan, Kateřina Zachová, Marek Buzáš, Pavel Pospíšil, Michal Křupka, Ankush PrasadFlow cytometric analysis Cell differentiation was assessed by evaluating the expression of surface CD markers using an SP6800 spectral cell analyzer (Sony Biotechnology, San Jose, California, USA) equipped with 405 nm, 488 nm

(59) PIK3CA inhibition in models of proliferative glomerulonephritis and lupus nephritisJournal of Clinical InvestigationAugust 1, 2024Junna Yamaguchi, Pierre Isnard, Noémie Robil, Pierre de la Grange, Clément Hoguin, Alain Schmitt, Aurélie Hummel, Jérôme Megret, Nicolas Goudin, Marine Luka, et al.information, please refer to the Supplemental Methods. Flow cytometry was performed using the Sony SP6800 Spectral Cell Analyzer, and data were analyzed with FlowJo software (TreeStar). Preparation of single-cell

(60) Metabolic adaptation pilots the differentiation of human hematopoietic cellsLife Science AllianceAugust 1, 2024Laëtitia Racine, Romuald Parmentier, Shreyas Niphadkar, Julie Chhun, Jean-Alain Martignoles, François Delhommeau, Sunil Laxman, Andras Paldi7-aminoactinomycin D marker (Invitrogen). The fluorescence intensity of CTV and antibodies was measured with the SP6800 cytometer (Sony) from the ImCy platform (Généthon). Cytometry on long-term cultures Cytometry results

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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer from Sony Corporation

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SP6800 Spectral Analyzer from Sony Corporation

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