Flow Cytometry

Flow Cytometry
Flow Cytometry Flow cytometry is a laser-based technology for rapidly characterizing populations of cells or proteins. As suspended particles flow through narrow stream and pass a detector, disruptions in a laser light beam or electricity flow are measured. Using the principles of light scattering, and the excitation and emission of fluorochrome molecules, a multi-parameter analysis of physical and chemical properties of single cells or particles in suspension can be calculated. Flow cytometry is a powerful high-throughput technique that is widely used for applications, such as immunophenotyping, cell sorting and isolation, cell cycle analysis, cell proliferation and cytotoxicity assays, biomarker detection, and protein engineering.

  • Flow Cytometry Panel Builder

    Flow Cytometry Panel Builder

    Careful planning of a multicolor flow cytometry experiment is crucial to ensure robust resolution, sensitivity, and biological accuracy. Our tool lets you design your flow panel using instrument configurations based on any flow cytometer of your choice.

Range of Flow Cytometry Products:

A wide variety of products are available for laboratories using flow cytometry, ranging from instruments to consumables. Flow cytometry kits contain prepackaged reagent sets and components for straightforward and optimized procedures, such as staining, labeling, or assay preparation. Reagents, such as beads, buffers and dyes, can be used to develop new lab-specific flow cytometry protocols.

  • Choices in Flow Cytometers

    Choices in Flow Cytometers

    Researchers now enjoy a wide selection of the latest flow cytometers from reputable manufacturers. What are some considerations when deciding on the appropriate instrument? All flow cytometers contain a flow cell, a light source (lamp or laser), a detector and a computer. Instruments will differ as to how many lasers and detectors they have. Most labs require the simultaneous detection of up to 6 fluorescent colors. However the number of applications for analyses based on numerous fluorophores is increasing, especially when multiplexing is involved. Determine how many unique parameters your lab needs to analyze and consider whether the instrument has additional features, such as the ability to capture a cell image or the faculty to sort them. Systems that include pre-programmed protocols, onboard imaging and customizable software will ease the workflow of a busy laboratory. Browse through our diverse catalog of cytometers and discover the one that is right for you.

Recommended Resources:

  • Flow Cytometry Strategies to Optimize Immunophenotyping

    Flow Cytometry Strategies to Optimize Immunophenotyping

    With immunotyping, certain cell types can be identified by just a single marker, but others require that multiple markers be used for characterization. We highlight some challenges of immunophenotyping and share flow cytometry strategies to help you succeed.
  • Flow Cytometry Dos and Don’ts for Multi-Omics Applications

    Flow Cytometry Dos and Don’ts for Multi-Omics Applications

    In this article, we summarize practical flow cytometry knowledge from three senior researchers and share their expertise in preparing cells and reagents, setting up assays, assessing instruments and controls, and sorting cells from various sample types.