SP6800 Spectral Analyzer from Sony Corporation

Product Specs
ItemSP6800 Spectral Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberSP6800
DetectionFSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
Lasers2 or 3 lasers
Excitation405nm (60mW), 488nm (40mW), 638nm (60mW)
Parameters64 fluorescence channels
CapacityTubes (5 mL)
Data Throughput10,000 events/sec; 20,000 events/sec (max)
Dimensions(WxDxH) 60.0 x 63.5 x 71.3 cm

(21) Resident alveolar macrophages are highly susceptible to the induction of RIPK1/RIPK3-mediated necroptosis which promote efficient pulmonary immune responseCell death & diseaseDecember 22, 2025Adachi T, Matsushita K, Yasuda K, Nakahira M, Imasaka M, Sugimoto M, Yang Z, Kobiyama K, Hayashi T, Ishii KJ, et al.Gr-1- and PerCP-Cy5.5, and propidium iodide (a viability dye) and then evaluated using a cell analyzer (SP6800; Sony Biotechnology, San Jose, CA). Immunization Ovalbumin (OVA, 10 μg) or recombinant type A influenza virus

(22) Oncostatin M induced by STAT5-activating oncogenes promotes disease progression in hematologic malignanciesSignal Transduct Target TherDecember 11, 2025Michael Rassner, Tony Andreas Müller, Kirstyn Anne Crossley, Geoffroy Andrieux, Sabina Schaberg, Cornelia Endres, Lena Jakob, Teresa Poggio, Natalie Köhler, Julia Kolter, et al.cell sorting Flow cytometry analysis was performed on a BD LSR Fortessa™, Beckman CyAn™ ADP, or Sony SP6800 Spectral Analyzer. The cells were sorted on a BD FACSAria III. The antibodies used to stain the following cell surface

(23) Peptide-MHC-targeted retroviruses enable in vivo expansion and gene delivery to tumor-specific T cellsScience advancesNovember 7, 2025Xu EJK, Smith BE, Conce Alberto WD, Walsh MJ, Lim B, Hoffman MT, Qiang L, Barreiro A, Finburgh EN, Dong J, et al.were then washed once with FACS buffer before analysis on a Cytoflex S (Beckman) or Sony Biotechnology SP6800 Spectral Analyzer. For experiments where absolute cell counts were calculated, CountBright Absolute Counting Beads (Thermo

(24) Platelet Jak2 deficiency accelerates atherosclerosis with increased inflammatory responseJournal of Biological ChemistryOctober 1, 2025Daeun Kim, Yu Zhe Li, Jiaqi Yang, Brian Lin, Meng Yun Wang, Jessie Jia Yu Yu Liu, Jia Qi Adam Adam Bai, Gedaliah Farber, Faisal Abdul Abdul Razzaq, Evan Pollock-Tahiri, et al.Samples were then diluted with 500 μl of Tyrode’s buffer lacking calcium and analyzed using a Sony SP6800 spectral cytometer. Platelet activation was quantified as the median fluorescence intensity of JON/A

(25) Dynamic expression of candidalysin facilitates oral colonization of Candida albicans in miceNature MicrobiologySeptember 25, 2025Ricardo Fróis-Martins, Julia Lagler, Tim B. Schille, Osama Elshafee, Kontxi Martinez de San Vicente, Sarah Mertens, Michelle Stokmaier, Iman Kilb, Natacha Sertour, Sophie Bachellier-Bassi, et al.Biolegend 123109, used at 1/100 dilution). Cells were fixed in 4.2% formaldehyde before acquisition on a SP6800 Spectral Analyzer (Sony). Data analysis was performed using FlowJo software v10 (FlowJo LLC). The gating of the flow cytometric

(26) Kupffer cells are essential for platelet-mediated thrombopoietin generation in the liverProceedings of the National Academy of Sciences of the United States of AmericaSeptember 23, 2025Karakas D, Li J, Ma W, Wu XG, Khoury CJ, Masoud M, Hoard B, Liu YJ, Zhu G, Chen J, et al.with 0.5 μg/mL anti-CD68 (FA-11) (Biolegend) for 1 h at room temperature. Cells were analyzed via Sony SP6800 flow cytometer. Hepatic Platelet Localization Confocal Microscopy. Mice were intraperitoneally injected

(27) Membrane receptors cluster phosphatidylserine to activate LC3-associated phagocytosisNature Cell BiologySeptember 22, 2025Emilio Boada-Romero, Clifford S. Guy, Gustavo Palacios, Luigi Mari, Suresh Poudel, Zhenrui Li, Piyush Sharma, Douglas R. Greenafter the probe, primary and secondary antibody incubations. Cell fluorescence was acquired using a SP6800 Sony Spectral Analyzer, and compensated values were analysed using the FlowJo v10 software. When cells

(28) Discovery of BMS-986408, a First-In-Class Dual DGKα and DGKζ Inhibitor that Unleashes PD-1 Checkpoint and CAR T-cell ImmunotherapiesCancer immunology researchSeptember 2, 2025Wichroski M, Liu SQ, Zasadil LM, Benci JL, Gedeon PC, Condon KJ, Joshi S, Posy S, Carlson P, Maier A, et al.then stained with antibodies listed in Supplementary Table S2, and samples were recorded using a Sony SP6800 spectral flow cytometer. Human peripheral blood mononuclear cell assay and synergy analysis Human

(29) HUMANIN produced by human efferocytic macrophages promotes the resolution of inflammationCell Death DisAugust 28, 2025Mélissa Maraux, Mathieu Vetter, Ludivine Dal Zuffo, Francis Bonnefoy, Audrey Wetzel, Alexis Varin, Baptiste Lamarthée, Olivier Tassy, Didier Ducloux, Philippe Saas, et al.diluted in PBS with 3% BSA (PAN BIOTECH) (wt/vol) at +4 °C. Cells were washed and analyzed on a Sony SP6800 Spectral Analyzer for human cells. For neutrophil apoptosis assay, cells were stained with FITC-conjugated Annexin-V and

(30) Effect of ultrasound-microbubble exposure on acute myeloid leukemia cancer cell proteomeSci RepAugust 14, 2025Joelle Stilwell, Daron A. Savaya, Emmanuel Sakarya, Charlotte Ferworn, Eleanor Cloves, Roberto J. Botelho, Costin N. Antonescu, Raffi KarshafianThermoFisher Scientific) at a concentration of 0.0001 mg/ml immediately before analysis with the SONY SP6800 flow cytometer (Sony Biotechnology, San Jose, CA, U.S.A). Flow cytometry analysis Flow Cytometry data

(31) Intradermally Administered Retinoic Acid or Vitamin D3-Loaded Liposomes Induce Tolerogenic Skin Dendritic CellsJ Immunol ResAugust 4, 2025Noémi Anna Nagy, Sanne G. Celant, Toni M. M. van Capel, Rinske Sparrius, Fernando Lozano Vigario, Ronald van Ree, Bram Slütter, Teunis B. H. Geijtenbeek, Sander W. Tas, Esther C. de Jong(Lonza) and 2% FCS (FACS buffer) with the markers listed in Table 3. Surface expression was measured on a SP6800 Sony Spectral Analyzer (Sony Biotechnology) or a FACS Canto II (BD). FlowJo software (Tree Star, Ashland,

(32) DR5 Disulfide Bonding Functions as a Sensor and Effector of Protein Folding StressMolecular Cancer ResearchJuly 2, 2025Mary E. Law, Zaafir M. Dulloo, Samantha R. Eggleston, Gregory P. Takacs, Grace M. Alexandrow, Young il Lee, Mengxiong Wang, Brian Hardy, Hanyu Su, Bianca Forsyth, et al.permeabilized. Stained samples were analyzed using single-color compensation and FMO controls on a Sony SP6800 spectral analyzer and quantified using FlowJo V10.8.1 (BD Biosciences, San Jose, CA). Cells were gated in the following

(33) DR5 Disulfide Bonding Functions as a Sensor and Effector of Protein Folding StressMolecular cancer research : MCRJuly 2, 2025Law ME, Dulloo ZM, Eggleston SR, Takacs GP, Alexandrow GM, Lee YI, Wang M, Hardy B, Su H, Forsyth B, et al.permeabilized. Stained samples were analyzed using single-color compensation and FMO controls on a Sony SP6800 spectral analyzer and quantified using FlowJo V10.8.1 (BD Biosciences, San Jose, CA). Cells were gated in the following

(34) Failing maternal-fetal tolerance in SLE (FaMaLE): a prospective cohort study for finding the molecular mechanisms behind pregnancy complicationsLupus Sci MedJune 19, 2025Wendy Dankers, Jikke F van Ruitenbeek, Serife Asya Germe, Agner R Parra Sánchez, Mirte F H M van Gaal, Marjolein Hortensius, Kyra Cramer, Daphne C Rohrich-Heldens, Marjon de Boer, Lisa G M van Baarsen, et al.with the intracellular antibodies in Perm buffer (eBioscience, ThermoFisher) and measured at the Sony SP6800. Data are analysed using FlowJo V.10.9.0. Statistical analysis All statistical tests will be performed

(35) A CAR enhancer increases the activity and persistence of CAR T cellsNature BiotechnologyJune 1, 2025Taha Rakhshandehroo, Shreya R. Mantri, Heydar Moravej, Benjamin B. V. Louis, Ali Salehi Salehi Farid, Leila Munaretto, Kathryn Regan, Radia M. M. Khan, Alexandra Wolff, Zoe Farkash, et al.(1:50, Biolegend) and recombinant BCMA–AlexaFluor647 (10 nM, made in-house). Samples were run on an SP6800 Spectral Analyzer (Sony, FCS express software) or Aurora (Cytek, SpectroFlo). The flow rate and acquisition time were

(36) Targeting the ZMYM2-ANXA9 Axis with FLT3 Inhibitor G749 Overcomes Oxaliplatin Resistance in Colorectal CancerBiomedicinesMay 20, 2025Dezheng Lin, Yucheng Xu, Huanmiao Zhan, Yufan Liang, Riyun Liu, Jun Liu, Dandong Luo, Xiaochuan Chen, Jiawei Cai, Yifeng Zoufollowing the manufacturer’s instructions. The stained cells were then analyzed by flow cytometry (SP6800, Sony, Tokyo, Japan) [32]. 2.8. Western Blotting Cultured cells were harvested using a scraper and

(37) Senolytic treatment to rescue hallmarks of senescence in lymph node fibroblasts from patients with rheumatoid arthritis: Implications for premature aging and potential therapeutic intervention in early rheumatoid arthritisClinical and Experimental ImmunologyMay 8, 2025T A de Jong, J F Semmelink, J W Bolt, C Grasso, R A Hoebe, P M Krawczyk, L G M van BaarsenAldrich, Zwijndrecht, The Netherlands]). Subsequently, LN fibroblasts were measured on a Spectral Analyzer SP6800 (Sony Biotechnology, Weybridge, United Kingdom) and analyzed using FlowJo 10.8.1 (TreeStar Inc., Ashland,

(38) Super-enhancer-driven EFNA1 fuels tumor progression in cervical cancer via the FOSL2-Src/AKT/STAT3 axisJournal of Clinical InvestigationApril 15, 2025Shu-Qiang Liu, Xi-Xi Cheng, Shuai He, Tao Xia, Yi-Qi Li, Wan Peng, Ya-Qing Zhou, Zi-Hao Xu, Mi-Si He, Yang Liu, et al.Fisher Scientific) for 30 minutes at room temperature. Cell cycle distribution was assessed using an SP6800 Spectral Analyzer (Sony) and analyzed using FlowJo v10 software. Cell apoptosis was evaluated through flow cytometry using

(39) CD45-PET is a robust, non-invasive tool for imaging inflammationNatureMarch 6, 2025Ali Salehi Salehi Farid, Jennifer E. Rowley, Harris H. Allen, Isabella G. Kruger, Soheil Tavakolpour, Kyle Neeley, Min Cong, Haneyeh Shahbazian, Niki Dorafshani, Achraf Berrada, et al.All probe concentrations were analysed in triplicate. Subsequent analyses were conducted using a Sony SP6800 Spectral Analyzer, with data analysis performed using FlowJo v.10.10 software. A curve was fitted for each binding curve,

(40) Recent Developments (After 2020) in Flow Cytometry Worldwide and Within ChinaBiosensors (Basel)March 2, 2025Xinyue Du, Xiao Chen, Chiyuan Gao, Junbo Wang, Xiaoye Huo, Jian Chenpatent and was further authorized by Sony to commercialize the breakthrough spectrum flow cytometry of SP6800. In 2017, Cytek caught up with Aurora and sparked a wave of technological competition in spectral flow cytometry

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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer from Sony Corporation

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SP6800 Spectral Analyzer from Sony Corporation

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