SP6800 Spectral Analyzer from Sony Corporation

Product Specs
ItemSP6800 Spectral Analyzer
CompanySony Corporation
Price Inquire
Catalog NumberSP6800
DetectionFSC, SSC, 32-channels (PMT arrays); cell position XY allows up to 64 channels
Lasers2 or 3 lasers
Excitation405nm (60mW), 488nm (40mW), 638nm (60mW)
Parameters64 fluorescence channels
CapacityTubes (5 mL)
Data Throughput10,000 events/sec; 20,000 events/sec (max)
Dimensions(WxDxH) 60.0 x 63.5 x 71.3 cm

(61) Transition of signal requirement in hematopoietic stem cell development from hemogenic endothelial cellsProceedings of the National Academy of SciencesJuly 30, 2024Saori Morino-Koga, Mariko Tsuruda, Xueyu Zhao, Shogo Oshiro, Tomomasa Yokomizo, Mariko Yamane, Shunsuke Tanigawa, Koichiro Miike, Shingo Usuki, Kei-ichiro Yasunaga, et al.CD8a-biotin), and further stained with streptavidin-AF700 (Fig. 2G). Stained cells were analyzed using an SP6800 spectral cell analyzer (SONY). Transplantation and Blood Analysis. Cells were intravenously injected

(62) Mechanisms of Resistance to Oncogenic KRAS Inhibition in Pancreatic CancerCancer DiscovJuly 5, 2024Julien Dilly, Megan T. Hoffman, Laleh Abbassi, Ziyue Li, Francesca Paradiso, Brendan D. Parent, Connor J. Hennessey, Alexander C. Jordan, Micaela Morgado, Shatavisha Dasgupta, et al.4°C, washed in PBS, and resuspended in 1% formalin for analysis on a spectral flow cytometer (Sony SP6800). Flow cytometry antibodies were purchased from Biolegend [αCD45 (30-F11), αCD4 (RM4-5), αCD8 (53-6.7),

(63) A liver immune rheostat regulates CD8 T cell immunity in chronic HBV infectionNatureJuly 1, 2024Bosch M, Kallin N, Donakonda S, Zhang JD, Wintersteller H, Hegenbarth S, Heim K, Ramirez C, Fürst A, Lattouf EI, et al.(Genaxxon). Flow cytometry and cell sorting Multicolour flow cytometry data were acquired on a Sony SP6800 spectral analyser (Sony Biotechnology) or a CytoFLEX S (Beckman Coulter). Cells were sorted with a Sony

(64) Synergistic Effects of Metal–Organic Nanoplatform and Guanine Quadruplex-Based CpG Oligodeoxynucleotides in Therapeutic Cancer Vaccines with Different Tumor AntigensVaccines (Basel)June 11, 2024Xia Li, Mitsuhiro Ebara, Naoto Shirahata, Tomohiko Yamazaki, Nobutaka HanagataPE-Cyanine7-anti-CD11c and APC-anti-H-2Kb of MHC class I bound to peptide SIINFEKL (BioLegend, San Diego, CA, USA). SP6800 spectral cell analyzer (Sony, Tokyo, Japan) was used for flow cytometry and FlowJo software was used

(65) PIK3CA inhibition in models of proliferative glomerulonephritis and lupus nephritisJ Clin InvestJune 6, 2024Junna Yamaguchi, Pierre Isnard, Noémie Robil, Pierre de la Grange, Clément Hoguin, Alain Schmitt, Aurélie Hummel, Jérôme Megret, Nicolas Goudin, Marine Luka, et al.information, please refer to the Supplemental Methods. Flow cytometry was performed using the Sony SP6800 Spectral Cell Analyzer, and data were analyzed with FlowJo software (TreeStar). Preparation of single-cell

(66) Small protein blockers of human IL-6 receptor alpha inhibit proliferation and migration of cancer cellsCell communication and signaling : CCSMay 7, 2024Groza Y, Lacina L, Kuchař M, Rašková Kafková L, Zachová K, Janoušková O, Osička R, Černý J, Petroková H, Mierzwicka JM, et al.Streptavidin-PE conjugate antibody was incubated for 30 min at RT. Samples were measured by the Sony SP6800 Spectral Cell Analyzer (Sony Biotechnology, San Jose, CA, USA) and the data was processed using FlowJo

(67) The Phytophthora parasitica effector AVH195 interacts with ATG8, attenuates host autophagy, and promotes biotrophic infectionBMC BiologyApril 29, 2024Serena Testi, Marie-Line Kuhn, Valérie Allasia, Pascaline Auroy, Fantao Kong, Gilles Peltier, Sophie Pagnotta, Julie Cazareth, Harald Keller, Franck PanabièresMegaPixel SIS Morada CCD camera (Olympus). Flow cytometry Analyses by flow cytometry were performed on a SP6800 spectral cytometer (SONY Biotechnologies). Chlamydomonas cells were labeled with 7.5 μM CFSE (Life technologies;

(68) Human cytomegalovirus exploits STING signaling and counteracts IFN/ISG induction to facilitate infection of dendritic cellsNature communicationsFebruary 26, 2024Costa B, Becker J, Krammer T, Mulenge F, Durán V, Pavlou A, Gern OL, Chu X, Li Y, Čičin-Šain L, et al.according to the intracellular labeling protocol from BD Bioscience. Then the samples were acquired on a SP6800 or ID7000 (Sony) and the data were analyzed using FlowJo software (Tree Star). RFI was calculated as

(69) MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified neuroblastomaCell Death & DiseaseFebruary 9, 2024Jinqiu Guan, Mengzhen Li, Yi Wang, Yu Zhang, Yi Que, Suying Lu, Juan Wang, Jia Zhu, Junting Huang, Zijun Zhen, et al.buffer, sequentially stained with Annexin V-FITC and PI, and immediately analyzed by flow cytometry (SP6800, Sony, Japan). Detection of NADPH/NADP+ and GSH/GSSG ratios, and ROS content The intracellular NADPH/NADP+

(70) AURKA inhibition induces Ewing’s sarcoma apoptosis and ferroptosis through NPM1/YAP1 axisCell Death & DiseaseJanuary 29, 2024Huimou Chen, Jing Hu, Xilin Xiong, Hongling Chen, Biaojun Lin, Yusong Chen, Yang Li, Di Cheng, Zhihua Li5 μL of Annexin V-Alexa Fluor 647 and 7-AAD for 5 mins away from light, followed by flow cytometry (SP6800, Sony, Japan) within 1 h. Western blotting (WB) The cells were washed with precooled PBS and lysed

(71) Modular cytosine base editing promotes epigenomic and genomic modificationsNucleic Acids ResearchJanuary 25, 2024Julian Weischedel, Laurence Higgins, Sally Rogers, Anna Gramalla-Schmitz, Paulina Wyrzykowska, Simone Borgoni, Thomas MacCarthy, Richard ChahwanFor live/dead discrimination cells were incubated with PI. GFP fluorescence was detected using a SONY SP6800 spectral analyser or BD FACS Fortessa. GFP-negative HEK293A and GFP-positive HEK293T-GFP cells were

(72) γδ T cells respond directly and selectively to the skin commensal yeast Malassezia for IL-17-dependent fungal controlPLOS PathogensJanuary 12, 2024Fiorella Ruchti, Meret Tuor, Liya Mathew, Neil E McCarthy, Salomé LeibundGut-Landmannappropriate. All staining steps were carried out on ice. Cells were acquired on a Spectral Analyzer SP6800 (Sony) or on a CytoFLEX S (Beckman Coulter), and the data were analyzed with FlowJo software (FlowJo

(73) A facile approach to preparing personalized cancer vaccines using iron-based metal organic frameworkFrontiers in ImmunologyJanuary 8, 2024Xia Li, Shinya Hattori, Mitsuhiro Ebara, Naoto Shirahata, Nobutaka Hanagataanti-H-2Kb- SIINFEKL-PE (BioLegend) antibodies. Flow cytometry was performed using a Spectral Cell Analyzer (SP6800, Sony). FlowJo software was used for the analysis of flow cytometry data. 2.7 Anti-tumor experiments

(74) Beyond 40 fluorescent probes for deep phenotyping of blood mononuclear cells, using spectral technologyFrontiers in immunologyJanuary 1, 2024Schmutz S, Commere PH, Montcuquet N, Cumano A, Ait-Mansour C, Novault S, Hasan M.different shapes of emission spectra became possible. With the first available spectral cytometer (Sony SP6800) equipped with two excitation lasers (488nm and 405nm) we built a panel of 19 fluorescent probes that

(75) Single-cell RNA-sequencing of PBMCs from SAVI patients reveals disease-associated monocytes with elevated integrated stress responseCell reports. MedicineDecember 19, 2023de Cevins C, Delage L, Batignes M, Riller Q, Luka M, Remaury A, Sorin B, Fali T, Masson C, Hoareau B, et al.extracellular staining has been performed for 30 min at 4°C protected from light. Samples were analyzed using the Sony SP6800 spectral flow cytometers. IFN-β stimulation of healthy PBMC Buffy coats from three healthy young male

(76) Multicenter randomized controlled trial of neoadjuvant chemoradiotherapy alone or in combination with pembrolizumab in patients with resectable or borderline resectable pancreatic adenocarcinomaJ Immunother CancerDecember 1, 2023Matthew H G Katz, Gina R Petroni, Todd Bauer, Matthew J Reilley, Brian M Wolpin, Chee-Chee Stucky, Tanios S Bekaii-Saab, Rawad Elias, Nipun Merchant, Andressa Dias Costa, et al.samples were fixed by adding 100 µL of 1% formalin. Flow cytometry analysis was performed using a SONY SP6800 Spectral Cell Analyzer (Sony Biotechnology, San Jose, California, USA). Statistical considerations

(77) Methamphetamine induces a low dopamine transporter expressing immunophenotype without altering total number of peripheral immune cellsBasic & Clinical Pharmacology & ToxicologyNovember 1, 2023Adithya Gopinath, Tabish Riaz, Emily Miller, Leah Phan, Aidan Smith, Ohee Syed, Stephen Franks, Luis R. Martinez, Habibeh KhoshboueiNumber,Purpose Centrifuge,Sorvall,ST8,PBMC isolation Cytometer,BD,Canto II,FC Spectral Analyzer,Sony,SP6800,FC Spectral Analyzer,Cytek,Aurora 5L,FC Flow cytometer,Beckman,Cytoflex LX,"FC, 13-plex ELISA" Microcentrifuge,Fisher,59A,FC Equipment

(78) PI3K/mTOR inhibitors promote G6PD autophagic degradation and exacerbate oxidative stress damage to radiosensitize small cell lung cancerCell Death & DiseaseOctober 6, 2023Huan Deng, Yamei Chen, Li Wang, Yibi Zhang, Qingqing Hang, Peijing Li, Peng Zhang, Jing Ji, Hai Song, Ming Chen, et al.at 488 nm. To evaluate the production of ROS MFI values in the live-cell gate were calculated using SP6800 Spectral Analyzer (Sony Biotechnology). When double peaks appeared, the right peak was believed to be the positive peak

(79) Preclinical characterization of WB737, a potent and selective STAT3 inhibitor, in natural killer/T‐cell lymphomaMedComm (2020)August 1, 2023Yali Wang, Wenbo Zhou, Jianfeng Chen, Jinghong Chen, Peng Deng, Huang Chen, Yichen Sun, Zhaoliang Yu, Diwen Pang, Lizhen Liu, et al.Bioscience) following the manufacturer's instructions. The stained cells were analyzed by flow cytometry (SP6800, Sony) and quantified by FlowJo software (BD Bioscience). 4.9 Colony formation assay A total of 2

(80) T cell migration and effector function differences in familial adenomatous polyposis patients with APC gene mutationsFrontiers in ImmunologyJuly 18, 2023Céline Cuche, Marta Mastrogiovanni, Marie Juzans, Hélène Laude, Marie-Noëlle Ungeheuer, Daniel Krentzel, Maria Isabella Gariboldi, Daniel Scott-Algara, Marianne Madec, Sophie Goyard, et al.cells were analyzed the following day by multiparameter flow cytometry using a Spectral Flow Cytometer SP6800 or ID7000 (Sony). Data analyses were performed with FlowJo software. Gating strategies for the different

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SP6800 Spectral Analyzer

SP6800 Spectral Analyzer from Sony Corporation

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SP6800 Spectral Analyzer from Sony Corporation

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