Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Activation-gated, T cell-restricted silencing of Dapk1 enhances Bacille Calmette-Guérin-elicited protective CD4+ memoryiScienceMay 15, 2026Zixuan Liu, Weihuang Liu, Chenyu Zhao, Xuanchang Bai, Fangting Zhou, Hang Sun, Jiahe Shi, Pinru Chen, Min Liu, Qin Pansubstrate,Biosharp,Cat# BL523B Zombie NIRTM Fixable Viability Kit,Biolegend,Cat# 423105 Fixation Buffer,Biolegend,Cat# 420801 Intracellular Staining Perm Wash Buffer(10×),Biolegend,Cat# 421002 Brefeldin A,Absin,Cat# abs810012 Cell lysis buffer

(2) Single-cell analysis highlights the significance of malignant cell IFN/MHC-II for immunotherapy response in head and neck squamous cell carcinomaCell Reports MedicineApril 21, 2026Michael Mints, Reilly A. Sample, Anuraag S. Parikh, Jesse M. Zaretsky, Zongtai Qi, Travis Law, Fudong Wang, Thomas F. Barrett, Riley Mullins, Ashley Reeb, et al.of phospho-STAT1, live cells were fixed at 37°C for 15 min in pre-warmed Fixation Buffer (BioLegend, 420801). Fixed cells were washed 3 times in PBS +0.5% BSA and subsequently permeabilized in pre-chilled True-Phos Perm Buffer

(3) Novel NK cell-like phenotype expressing CCR5 and its ligands elicits tumor-specific acquired immunity to carcinomatous peritonitis via host NK-derived IFN-γJ Immunother CancerApril 20, 2026Situo Zheng, Yui Harada, Yosuke Morodomi, Noriko Yasuda, Kenta Ishimoto, Yoshikazu YonemitsuUSA). After 6 hours, following surface staining, the cells were fixed with fixation buffer (BioLegend, 420801, California, USA) for 20 min at room temperature, then washed two times using 1x Intracellular Staining

(4) Sunitinib induces immunogenic cell death through eIF2α phosphorylation to potentiate immunotherapy in HCCiScienceApril 17, 2026Yimei Gu, Hanbing Mai, Peijian Huang, Hui Yuan, Mingxuan Sun, Huixin Gao, Chenyu Shang, Yangfen Ou, Jizhou Tan, Xianzhang Huang, et al.incubation, cells were washed for intracellular staining. Cells were then fixed with Fixation Buffer (Cat#420801, BioLegend) and permeabilized with Permeabilization Buffer (Cat#00-8333-56, Invitrogen). Cells were

(5) Enhanced Th1 Cellular Immunity Induced by an RSV-F mRNA Vaccine Rationally Designed Using NLP AlgorithmsVaccines (Basel)April 16, 2026Zhi-Wu Xia, Qi Tang, Jun-Jie Pan, Jing Liu, Lan-Xin Jia, Guo-Mei Zhang, Man-Ni Xie, Jia-Hao Zheng, Chuan-Shuo Lv, Lei Zhang, et al.CD8 (Biolegend, Cat. #100706) for 15–30 min at RT. After fixation (Biolegend Fixation Buffer, Cat. #420801) and permeabilization (Biolegend Intracellular Staining Permeabilization Wash Buffer, Cat. #421002),

(6) Phenanthrene Exposure Induces Immune Dysfunction and Metabolic Disruption in MiceACS OmegaApril 15, 2026Qiaojuan Liu, Yan Zhang, Fenfang Deng, Wenxue Li, Guangyu Yang, Jun Yuan, Yuehong Wei, Juntao Li, Lei Tanand permeabilized using the Intracellular Fixation/Permeabilization Buffer Set (BioLegend, Cat. No. 420801/421002), followed by staining with fluorochrome-labeled antibodies against IFN-γ and TNF-α. All stained

(7) Payload Diversification Overcomes Resistance and Guides Sequential Antibody–Drug Conjugate Therapy in Breast CancerClinical Cancer ResearchApril 15, 2026Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Takeo Fujii, Chalothorn Wannaphut, Jennifer A. Maynard, Kyoji Tsuchikama, George W. Sledge, et al.then replaced with antibody-free culture medium, cells were fixed with fixation buffer (BioLegend, 420801), mounted with DAPI-containing medium (ibidi, 50011), and 0 h images were acquired. A parallel plate

(8) EBV Triggers a Distinct Antiviral Response in HMC3 CellsbioRxivApril 7, 2026Noah E. Berkowitz, Alexander Nosov, Mark Nosov, Fryda Solis Roldan, Anuj Ahuja, Miranda McGaskey, Ethel Cesarman, Douglas F. Nixon, Nicholas Dopkinsroom temperature conditions using a fixation buffer containing 4% paraformaldehyde (BioLegend, Cat# 420801). Fixation buffer was then decanted and 200μL of DAPI Fluoromount-G® (SouthernBiotech, Cat# 0100–20)

(9) A periplasmic protein complex mediates arabinofuranosyltransferase activity and intrinsic drug resistance in Mycobacterium tuberculosisSci AdvApril 3, 2026Thaís Klevorn, Christopher Brown, Christine D. Hardy, Bonnie J. Cuthbert, Amanda Spencer, Adrián Jinich, Luming Chan, Shiva K. Angala, Jordan Manzer, Jessica Mendoza, et al.supernatant containing DMN-Tre was discarded. Samples were fixed with 4% paraformaldehyde (PFA; BioLegend, #420801) for 4 hours at room temperature. After two washes in PBS with 0.05% Tween 80, bacteria were resuspended

(10) Krüppel-like factors 2 and 3 regulate T cell exhaustion by directing T cell residency and migrationImmunityMarch 27, 2026Jian Shen, Ryan J. Brown, Yuqi Zhang, Kexin Gai, Ashley K. Brown, Ashley M. Bauer, Ashley Zhu, Siying Lin, Hongshen Niu, Arjun Kharel, et al.19853 P3 Primary Cell 96-well Nucleofector Kit,Lonza,Cat# V4SP-3096 Fixation Buffer,BioLegend,Cat# 420801 True-Nuclear Transcription Factor Buffer Set,BioLegend,Cat# 424401 Foxp3 / Transcription Factor Staining

(11) Recombinant human adenovirus type 5 synergizes with anti-PD-L1 antibody to promote anti-hepatocellular carcinoma effects through multilevel remodeling of the immune microenvironmentFrontiers in ImmunologyMarch 24, 2026Qiu Zhao, Min Xiao, Jian Ma, Xiaoqian Li, Cong Fu, Wenjing Ji, Lingna Ni, Jialin Fan, Qianqian Gao, Yanzhi BiAPC-CD86 (BioLegend, 105011), Alexa Fluor® 700-CD206 (BioLegend, 141733), fixation buffer (BioLegend, 420801), and intracellular staining permeabilization wash buffer (BioLegend, 421002). 2.7 HE, immunohistochemistry The

(12) Peripheral immune landscape in pancreatic ductal adenocarcinoma reveals expansion of effector states with disease progressioniScienceMarch 20, 2026Chen-Hsuan Yang, Yun-Yi Lung, Chieh-Chang Chen, I-Hsuan Lo, Chia-Jung Lien, Wei-Kai Wu, Ming-Shiang Wu, Wei-Chih Liao, Jian-Da Linstain,NanoEnTek,Cat# EBT-001 Brilliant Stain Buffer,BD Biosciences,Cat# 566385 Fixation Buffer,Biolegend,Cat# 420801 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, No critical

(13) Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicronJ ImmunolMarch 18, 2026Robert W P Glowacki, Jessica M Till, Orion D Brock, Vladimir Makarov, Morgan J Engelhart, Philip P Ahernby pipetting in 100 µl of a commercially available formaldehyde-based fixative (BioLegend, Cat. no. 420801; used when no intracellular staining was subsequently performed) or (ThermoFisher, Cat. # 00-5123-43

(14) Comprehensive single-cell metabolic profiling identifies targets to sensitize triple-negative breast cancer to chemo-immunotherapyCell Rep MedMarch 17, 2026Ying Xu, Xin-Yi Liu, Hang Zhang, Li Chen, Han Wang, Zhi-Ming Shao, Yi Xiao, Yi-Zhou Jiang420302 Intracellular Staining Permeabilization Wash Buffer (10x),BioLegend,Cat# 421002 Fixation Buffer,BioLegend,Cat# 420801 Cyto-Last™ Buffer,BioLegend,Cat# 422501 Cell Staining Buffer,BioLegend,Cat# 420201 Opal Polaris 7-Color

(15) Selenium-loaded sustained-release schizophyllan alleviates pancreatic and pulmonary inflammatory damage in type 1 diabetes mellitus by modulating gut microbiota and T cell balanceJournal of NanobiotechnologyMarch 14, 2026Junming Luo, Siying Pei, Zhiying Ai, Runqing Rao, Zhuoyuan Yang, Xin Guo, Zeyi Ai, Lilin Fan, Fang ZouIntracellular staining was performed after permeabilization with fixation/permeabilization concentrate (420801, BioLegend, USA) mixed with diluent at a 1:3 ratio, followed by treatment with permeabilization buffer

(16) Prolonged Wnt3a exposure tolerizes macrophages to inflammatory stimuliFront ImmunolMarch 12, 2026Megan L. Tigue, Rincon Jagarlamudi, Channing Chi, Diana Diaz, Hua-Chang Chen, Quanhu Sheng, Sheau-Chiann Chen, Anna Schwarzkopf, Jeremy A. Goettel, Heather H. Pua, et al.temperature in the dark. After surface staining, cells were fixed with Fixation buffer (BioLegend, 420801) for 20 minutes in the dark and resuspended in 1X Intracellular Staining Permeabilization Wash Buffer

(17) Site-specific profiling of structure and function of Igµ B cell receptor glycansNature CommunicationsMarch 5, 2026M. D. Holborough-Kerkvliet, L. Hafkenscheid, S. Kroos, R. Biersteker, R. van de Wetering, O. Singh, E. Fadda, R. T. N. Tjokrodirijo, P. A. van Veelen, M. Wuhrer, et al.F(ab’)2 (Southern Biotech, #2062-01 and #2072-01, respectively) at 37 ⁰C. Fixation buffer (BioLegend, #420801) was used to fix the cells and was added immediately after the stimulation time passed. Cells were washed

(18) NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumorsNature CommunicationsFebruary 27, 2026Dominik Humer, Victoria Klepsch, Dietmar Rieder, Isabel Hölzl, Daniel Schreiber, Viktor Lang, Jiří Koutník, Sebastian Peer, Tajana Sajinovic, Viana Wille, et al.surface antibodies as stated above. Thereafter cells were fixed using the fixation buffer from Biolegend (420801) for 20 minutes at RT. After fixation, cells were permeabilized by washing twice with the kit’s Perm/Wash

(19) Investigating the Immune Effects of Radiotherapy in Non-Small Cell Lung Cancer—Results of the PD-RAD StudyOncology ResearchFebruary 24, 2026Shuhui Cheng, Tiana Kordbacheh, Antonia Banyard, Anshuman Chaturvedi, Diego Sanchez Martinez, Crispin T. Hiley, Maggie Harris, Clara Chan, Corinne Faivre-Finn, Timothy M. Illidge, et al.were washed in CSB and fixed in 1 mL of 4% paraformaldehyde (PFA) in PBS (fixation buffer, Biolegend, 420801) per 3 × 106 cells, for a minimum of 18 h. Finally, per 3 × 106 cells, 1 µL of iridium solution (10

(20) Long-acting interleukin-7 improves the efficacy of oncolytic viral therapy in glioblastomaNature CommunicationsFebruary 17, 2026Yuping Derek Li, David A. Giles, Yi Huang, Ashwani Kesarwani, Tong Hu, Logan Page, Amanda de Andrade Costa, Jingqin Luo, Alexandra Wolfarth, Donghoon Choi, et al.controls were used for setting population gates. For all staining procedures, cells were fixed (Biolegend, 420801), and permeabilization (Biolegend, 421002) was performed if intracellular staining was necessary. Absolute

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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