Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC,ICC
Quantity100ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) High-Density Type I Collagen Promotes IFN-γ+ CD8+ T Cell Exhaustion via SAT1-ASS1-Mediated Glutamine Accumulation and Ferroptosis in Triple-Negative Breast CancerAdv Sci (Weinh)September 27, 2026Jinyan Wang, Shuangyue Pan, Lu Bai, Bin Li, Tiantian Liu, Jianing Cao, Mengdi Yang, Heda Zhang, Duancheng Guo, Wenxiang Zhi, et al.antibodies against cell surface markers, cells were fixed and permeabilized using the relevant buffer set (420801, 421002, BioLegend, USA) for 20 min to disrupt the cell membrane. They were then treated with fluorochrome‐conjugated

(2) Deciphering senescence-associated mechanisms through cell painting & transcriptomicsNPJ AgingSeptember 17, 2026Yuliana Tan, Anika Liu, Emily Larkin, Revati Wani, Alec Dick, Stefano Patassini, Patrik Erlmannfor live imaging were washed with DPBS (Gibco, 14040-117) and fixed with Fixation Buffer (Biolegend, 420801) for 10 min at room temperature. After fixation, cells were washed with 1% BSA in DPBS and incubated

(3) Lactobacillus salivarius potentiates gastrointestinal cancer immunotherapy through its metabolite chenodeoxycholic acidCell Reports MedicineSeptember 15, 2026Bohan Zhang, Yifan Zhang, Yi Cai, Siyuan Cheng, Zihan Han, Xiaochen Yin, Tong Xie, Yan Tan, Yang Feng, Yuxin Wang, et al.RRID:AB_2734492 Zombie Aqua™ Fixable Viability Kit,BioLegend,Cat#423101; RRID:N/A Fixation Buffer,BioLegend,Cat#420801; RRID:N/A Zombie Aqua™ Fixable Viability Kit,BioLegend,Cat#423101; RRID:N/A GAPDH,Abcam,Cat#2118;

(4) PLP1-triggered NOT gate to address neurotoxicity risk of PSMA-targeted prostate cancer therapyJ Immunother CancerSeptember 12, 2026Sanam Shafaattalab, Alexander Partin, Aaron Winters, Richele Bruno, Vasantika Suryawanshi, Jushen Liang, Ryan Elshimali, Nicolas G Pedroncelli, Chawita Netirojjanakul, Timothy P Riley, et al.seeded in 96-well plates several days before staining. Cells were fixed in fixation buffer (BioLegend #420801) and permeabilized in BD Phosflow Perm/Wash Buffer I (BD#557885). They were then blocked in 5% BSA (Bioworld#

(5) Pharmacological Inhibition of Small Extracellular Vesicle Secretion by ALK5i SD-208 via Lysosomal Rerouting of CD63+ CompartmentsJ Extracell VesiclesAugust 29, 2026Rahul Sanwlani, Georgina H. Thompson, Kyle Bramich, Ching-Seng Ang, Millie L. Trowbridge, Chris E. Duringer, Vlad Stolojan, Sean M. Davidson, Konstantinos Savvatis, John H. McVey, et al.Cell Staining Buffer. For intracellular staining, samples were fixed in 500 µL Fixation Buffer (BioLegend, 420801) for 20 min, then centrifuged at 400 × g for 5 min. These samples were subsequently washed and permeabilised

(6) A generalizable covalent car-t platform for solid tumors enabled by oncolytic adenovirus-delivered artificial antigensFrontiers in ImmunologyAugust 26, 2026Linpei Guo, Hui Xie, Di Yin, Guidong Zhu, Peichao Zhai, GuoJing Pei, Wen Zhang, Fang Xiao, Jiaju Lyu, Dongqi Tangstaining, the cells were incubated with brefeldin A (BioLegend # 420601) for 12 h, fixed (BioLegend, #420801), permeabilized (BioLegend, #421002), and processed according to the manufacturer’s instructions. Cells

(7) Galanin impairs tumor immunity in glioblastoma by promoting infiltration and ferroptosis resistance of myeloid-derived suppressor cellsNature CancerAugust 25, 2026Lizhi Pang, Yang Liu, Fei Zhou, Songlin Guo, Fatima Khan, Heba Ali, Hardik Shah, Sara Huber, Barbara Kofler, Craig M. Horbinski, et al.to MDSCs for 10 min on ice. Following washing with PBS and fixing with fixation buffer (BioLegend, 420801), the signal of the ferroptotic MDSCs was read in a BD FACSymphony A5 flow cytometer. Cell viability

(8) H3.3 chaperone Hira primes the effector program and function of regulatory T cellsSci AdvAugust 21, 2026Xiangxiang Cao, Mengjie Lv, Zhihan Lv, Shuting Zheng, Xinyi Tan, Jiyu Ding, Wei Liang, Guohong Li, Xuyu Zhou, Mingzhao Zhu1640 for 20 min at 37°C in a 5% CO2 incubator. Cells were subsequently fixed with a Fixation Buffer (420801, Biolegend) and permeabilized with a True-Phos Perm Buffer (425401, Biolegend) according to the manufacturer’s

(9) Pulmonarom® promotes TLR-related and interferon-related antiviral responses during influenza A (H1N1 and H3N2) infection in human monocyte-derived dendritic cellsFrontiers in Cellular and Infection MicrobiologyAugust 18, 2026Sandra Georgina Solano-Gálvez, Juan A. Hernández-Aceves, Alberto García Lozano, Arturo A. Wilkins-Rodríguez, Diego Alexander Rojas Ortega, Antonio Ibarra, Exsal Manuel Albores Méndez, Edda Sciutto, Laila Gutiérrez-Kobeh, Horacio Merchant Larios, et al.criterion before staining. After surface staining, cells were fixed with Fixation Buffer (BioLegend, Cat. 420801) for 20 min at room temperature, then washed with 1× Wash Buffer (BioLegend, Cat. 421002) and centrifuged

(10) Basophil-specific deletion of interleukin 15 impairs memory CD8+ T cell homeostasis in the bone marrowFrontiers in ImmunologyAugust 18, 2026Carmen Stecher, Ines Neuwirth, Amra Begunic, Nina Braun, Dietmar Herndler-Brandstetterstep, cells were fixed for 15 minutes using a paraformaldehyde-based fixation buffer (Biolegend, Cat# 420801) and permeabilized according to the manufacturer’s instructions. Intracellular antibodies were stained

(11) Enhancing iNKT cell immunotherapy through the integration of optimized CAR endodomains and iNKT engagersNature CommunicationsAugust 14, 2026Kanagaraju Ponnusamy, Klesti Karaxhuku, Yuchao Jiang, Lyra Randzavola, Hongwei Ren, Ilia Leontari, Bryan Lye, Mehmood Zaidi, Edward J. Bartlett, Edward W. Tate, et al.cells were fixed and permeabilized by following standard manufacturer’s instructions (BioLegend, cat# 420801 and 421002). The cells were stained with granzyme B (clone: GB11) and IFNγ (clone: 4S.B3) in FACS buffer

(12) Dissociation method influences immune cell composition in porcine tonsil cell suspensionsFront ImmunolAugust 13, 2026Luise Hohensee, Alexa Eisenmann, Emma L. Lemberger, Xin Qu, Tanja Groll, Katja Steiger, Friederike EbnerFACS buffer at a staining volume of 30µL per sample. For intracellular staining, the fixation buffer (420801, Biolegend, USA) was used according to manufacturer’s instructions, intranuclear fixation was achieved

(13) AFF3 maintains metabolic quiescence in naïve CD8 T cells and prevents premature immune agingJCI InsightAugust 10, 2026Molly E. Lumnitzer, Stefanie F. Valbon, Stephanie A. Condotta, Allison E. Norlander, Sheng Liu, Jun Wan, Martin J. Richerat 4°C for 20 minutes, followed by another wash. Cells were treated with fixation buffer (BioLegend, 420801) and incubated at 4°C for 10 minutes, followed by a wash. Cells were resuspended in FACS buffer and

(14) Human bronchial air-liquid interface responses to nebulised protease exposureFront ImmunolAugust 7, 2026Georgina Hopkins, David Sheffield, Hugh Barlow, Anja Lalljie, Victoria James, Stella Cochrane, Lucy C. FaircloughPBS; 200 µL apically and 750 µL basally. Cells were then fixed with 4% paraformaldehyde (v/v) (Cat no. 420801, Biolegend), 200 µL applied apically and 750 µL basally, for 30 minutes at room temperature. The fixation

(15) Localized immunomodulation with cytokine-producing cells to mitigate foreign body responses in rodents and a nonhuman primateSci AdvAugust 7, 2026Boram Kim, Dilrasbonu Vohidova, Amanda Nash, Yuen San Chan, Samantha Fleury, Shravani Deo, Danna Murungi, Peter D. Rios, Ira Joshi, Hafsa Nasir, et al.with cell staining buffer and fixed for an hour at room temperature with fixation buffer (catalog no. 420801, BioLegend). After fixation, cells in each sample were resuspended in cell staining buffer and run through

(16) Cblb Gene Editing in T Cells Sustains Expansion and Immunogenic CAR T Tumor Killing Under Chronic Antigenic StimulationCellsAugust 3, 2026Daniel Schreiber, Sebastian Peer, Christina Lutz-Nicoladoni, Jiří Koutník, Viktor Lang, Viana Wille, Isabel Hölzl, Dominik Humer, Nino Tokic, Dorothee Freimark, et al.AB_2561389). After staining, cells were washed with buffer C and fixed in a Fixation Buffer (BioLegend, 420801) for 20 min at RT. Subsequently, cells were washed twice with 1× Intercellular Staining Permeabilization

(17) Modeling stimulus-induced stress responses in microglia-like cells using a commercial iPSC-dCas9-KRAB lineStem Cell ResearchAugust 1, 2026Devin M. Saunders, Nicola A. Kearns, Bernard Ng, Faraz Sultan, Himanshu Vyas, Ricardo A. Vialle, Eric M. Clark, Sashini De De Tissera, Jishu Xu, David A. Bennett, et al.min, then washed twice with FACS buffer. Stained cells were fixed with Fixation Buffer (Biolegend, 420801) according to the manufacturer’s instructions. If applicable, fixed cells were permeabilized using Intracellular

(18) ST6GAL1 Glycoengineering Rewires Cytokine Signaling and Preserves Metabolic Fitness in CAR-T Cells Under Galectin-3-Mediated ImmunosuppressionInt J Mol SciJuly 18, 2026Lee Seng Lau, Maria Suarez, Aizada Berdalinova, Rebecca Z. Fan, Rajib Kumar Shil, Joseph Souchak, Kim Tieu, Avery D. Posey, Charles J. DimitroffFollowing stimulation, cells were fixed via the addition of pre-warmed Fixation Buffer (BioLegend; Cat. No. 420801, San Diego, CA, USA) and incubated for 15 min at 37 °C to preserve the phosphorylation status. Cells

(19) Naive CD4+ T cells synthesize a hyaluronan-rich glycocalyx that is diminished following activationiScienceJuly 17, 2026Laxmi Prasad Adhikari, Jessica Contreras, Kristen M. Patterson, Meghan M. Moran, Erin N. Howe, Anna H.K. Plaas, Joseph M. ReynoldsEasySep Mouse CD4+ T cell Isolation Kit,STEMCELL Technologies,Cat#: 19852 Fixation Buffer,Biolegend,Cat#: 420801 M-MLV Reverse Transcriptase,Thermo Fisher Scientific,Cat#: 28025013 ,, Deposited data,Deposited data,Deposited

(20) Dendritic cell redundancy enables priming of anti-tumor CD4+ T cells in pancreatic cancerCancer CellJuly 13, 2026Courtney T.S. Kureshi, Michael J. Walsh, Rakeeb Kureshi, Victoire Cardot-Ruffino, Dennis A. Agardy, Lestat R. Ali, James M. Dougan, Li Qiang, Jiao Shen, Chong Zuo, et al.for 5 min. After discarding supernatants, cells were incubated in 200 μL Fixation Buffer (BioLegend #420801) for 20 min at room temperature. Cells were centrifuged at 700 g for 5 min. Supernatants were discarded

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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