Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Localized immunomodulation with cytokine-producing cells to mitigate foreign body responses in rodents and a nonhuman primateSci AdvAugust 7, 2026Boram Kim, Dilrasbonu Vohidova, Amanda Nash, Yuen San Chan, Samantha Fleury, Shravani Deo, Danna Murungi, Peter D. Rios, Ira Joshi, Hafsa Nasir, et al.with cell staining buffer and fixed for an hour at room temperature with fixation buffer (catalog no. 420801, BioLegend). After fixation, cells in each sample were resuspended in cell staining buffer and run through

(2) Modeling stimulus-induced stress responses in microglia-like cells using a commercial iPSC-dCas9-KRAB lineStem Cell ResearchAugust 1, 2026Devin M. Saunders, Nicola A. Kearns, Bernard Ng, Faraz Sultan, Himanshu Vyas, Ricardo A. Vialle, Eric M. Clark, Sashini De De Tissera, Jishu Xu, David A. Bennett, et al.min, then washed twice with FACS buffer. Stained cells were fixed with Fixation Buffer (Biolegend, 420801) according to the manufacturer’s instructions. If applicable, fixed cells were permeabilized using Intracellular

(3) ST6GAL1 Glycoengineering Rewires Cytokine Signaling and Preserves Metabolic Fitness in CAR-T Cells Under Galectin-3-Mediated ImmunosuppressionInt J Mol SciJuly 18, 2026Lee Seng Lau, Maria Suarez, Aizada Berdalinova, Rebecca Z. Fan, Rajib Kumar Shil, Joseph Souchak, Kim Tieu, Avery D. Posey, Charles J. DimitroffFollowing stimulation, cells were fixed via the addition of pre-warmed Fixation Buffer (BioLegend; Cat. No. 420801, San Diego, CA, USA) and incubated for 15 min at 37 °C to preserve the phosphorylation status. Cells

(4) Naive CD4+ T cells synthesize a hyaluronan-rich glycocalyx that is diminished following activationiScienceJuly 17, 2026Laxmi Prasad Adhikari, Jessica Contreras, Kristen M. Patterson, Meghan M. Moran, Erin N. Howe, Anna H.K. Plaas, Joseph M. ReynoldsEasySep Mouse CD4+ T cell Isolation Kit,STEMCELL Technologies,Cat#: 19852 Fixation Buffer,Biolegend,Cat#: 420801 M-MLV Reverse Transcriptase,Thermo Fisher Scientific,Cat#: 28025013 ,, Deposited data,Deposited data,Deposited

(5) Dendritic cell redundancy enables priming of anti-tumor CD4+ T cells in pancreatic cancerCancer CellJuly 13, 2026Courtney T.S. Kureshi, Michael J. Walsh, Rakeeb Kureshi, Victoire Cardot-Ruffino, Dennis A. Agardy, Lestat R. Ali, James M. Dougan, Li Qiang, Jiao Shen, Chong Zuo, et al.for 5 min. After discarding supernatants, cells were incubated in 200 μL Fixation Buffer (BioLegend #420801) for 20 min at room temperature. Cells were centrifuged at 700 g for 5 min. Supernatants were discarded

(6) Senescence-associated loss of intestinal α1,2-fucose disrupts a modifiable host-microbiome homeostasis axis in people with HIVbioRxivJuly 10, 2026Leila B. Giron, Maliha W. Shaikh, Thaisa M Cantu Cantu Jungles, Lijuan Zhang, Phillip A. Engen, Nuseybe Bulut, Shalini Singh, Jenna M. Hasson, Ellen Zhang, Shivanjali Shankaran, et al.Cells were then washed to remove unbound antibody and fixed with paraformaldehyde (BioLegend, catalog# 420801). Data were acquired on a FACSymphony A5 Spectral Analyzer. Measurement of plasma markers of microbial

(7) Protocol to purify human and mouse cytokines using an endotoxin-free Escherichia coli-based platformSTAR ProtocJuly 9, 2026Jae-Yoon Kim, Jessica Xin, I-Chih Kuo, Si Xuan Chen, Ahmed Kabil, Kai-Wei Chang, Nika Shakiba, Kelly M. McNagny, Yanpu HeMouse Recombinant IL-33,BioLegend,580506 Recombinant Human IL-33 (carrier-free),BioLegend,581804 Fixation buffer,BioLegend,420801 Recombinant mouse LIF,R&D Systems,8878-LF MEK inhibitor (PD0325901),Tocris Bioscience,4192/10 Embryonic stem cell

(8) Pink1-mediated mitophagy in the endothelium releases proteins encoded by mitochondrial DNA and activates neutrophil responses during inflammationElifeJuly 1, 2026Priyanka Gajwani, Li Wang, Koushik Debnath, Pierina Danos, Young-Mee Kim, Shubhi Srivastava, Zijing Ye, Sarah Krantz, Dong-Mei Wang, Chinnaswamy Tiruppathi, et al.with gentle shaking. Cells were fixed by adding an equal volume of Fixation buffer (Biolegend Cat# 420801) for 10 min, washed, and then analyzed by flow cytometry. For endothelial ICAM-1 expression, the median

(9) Hepatic Arachidonic Acid Dysregulation Impairs Double-Negative Regulatory T Cell-Mediated Immunoregulation via CD39 Downregulation and Drives MASLD DevelopmentJ Immunol ResJune 25, 2026Hua Jin, Xiyu Wang, Xinjuan Liu, Dong Zhang, Guangyong Sun(53‐6.7), and anti‐CD39 (Duha59). For intracellular staining, the cells were fixed with fixation buffer (420801, BioLegend) and subsequently stained with either anti‐granzyme B (QA16A02) or anti‐TNF‐α (MP6‐XT22).

(10) Intermediate accumulated upon interruption of fatty acid oxidation flux promotes tumoral ferroptosis and improves immunotherapyNature CommunicationsJune 17, 2026Yuhan Zhou, Jing Li, Fangfang Liu, Yuan Gao, Songlin Yin, Liguo Yang, Xiaoxiao Li, Junhong Lin, Yan Li, Haotian Shang, et al.Biolegend) for 30 min at room temperature. Cells were washed, fixed and permeabilized by Fixation Buffer (420801, Biolegend) and Perm/Wash Buffer (421002, Biolegend), respectively. Then, cells were stained with anti-IFNγ

(11) Case Report: Immune checkpoint inhibitor-associated myocarditis, myositis, and myasthenia gravis overlap syndrome with flow cytometric phenotyping before and after treatment in a patient with urothelial carcinomaFrontiers in ImmunologyJune 15, 2026Karen Gambina, Carly Tymm, Matthieu Paiola, Kurenai Tanji, John Zech, Robert Winchester, Adam Mor, Yevgeniya GartshteynWaltham, Massachusetts, USA). Cells were then fixed with 4% paraformaldehyde Fixation Buffer (BioLegend #420801) and treated with Intracellular Staining Fixation Buffer (BioLegend 420801 and 421002) before intracellular

(12) The Effects of CB2R Activation on Inflammatory Pathways in DermatomyositisBiomedicinesJune 7, 2026Rohan Dhiman, Ahmed Eldaboush, Navin Vijayarangan, Darae Kang, Nilesh Kodali, DeAnna Diaz, Caroline Stone, Rui Feng, Victoria P. Werth30 min, after which the cells were washed with PBS and fixed with fixation buffer (Biolegend USA, #420801). This was then followed by resuspension in permeabilization buffer (Biolegend USA, #421002). The following

(13) NMI Regulates Adipose Adaptive Thermogenesis Through TLR4/IRF3 Signaling to Promote ObesityAdvanced ScienceJune 2, 2026Ting‐Ting Li, Xin‐Yuan Zhao, Min Zhang, Zhuang‐Feng Weng, Xiao‐Ran Guo, Ying‐Fang Liu, Qiao‐Ping Wang, Huan‐Huan LiangFor intracellular marker staining, cells were first fixed with fixation buffer (Biolegend, USA, Cat#420801) at 4°C for 30 min, followed by incubation with antibodies diluted in intracellular permeabilization

(14) Multimodal sequencing identifies synergistic mechanisms driving resistance to neoadjuvant nivolumab treatment in hepatocellular carcinomaMolecular CancerJune 1, 2026Fanhong Zeng, Jiaxin Guo, Zheng Zeng, Vanilla Xin Zhang, Yu-Man Tsui, Po-Man Li, Abdullah Husain, Qingyang Zhang, Joyce Man-Fong Lee, Tan-To Cheung, et al.co-cultured at a 10:1 effector-to-target ratio for 48 h. THP1 were collected and fixed with fixation buffer (420801, BioLegend), followed by incubation with the following antibodies: CD68 (333809, BioLegend), CD86 (305406,

(15) Non-decameric NLRP3 reveals a TGN/MTOC-distal pathway of inflammasome activationNature CommunicationsMay 30, 2026María Mateo-Tórtola, Inga V. Hochheiser, Gaopeng Li, Lukas Funk, Atousa Hashemi, Xiao Liu, Jane Torp, Lena Erlebach, András Szolek, Jelena Grga, et al.by the Zen blue software. In brief, cells plated in coverslips (12 mm) were fixed in 4 % PFA in PBS (420801, Biolegend) at 37 °C for 15 min, then washed in PBS 3 times with 5 min interval between washes. Afterwards,

(16) Impact of liquid dosing and associated cell ‘drowning’ in a 3D human bronchial epithelial model used for protease exposure studiesFrontiers in ToxicologyMay 27, 2026Georgina Hopkins, David Sheffield, Hugh Barlow, Anja Lalljie, Stella Cochrane, Lucy C Faircloughwith PBS; 200 µL apically and 750 µL basally. Cells were then fixed with 4% paraformaldehyde (Cat no. 420801, Biolegend), 200 µL applied apically and 750 µL basally, for 30 min at room temperature. The fixation

(17) A glycan-based adjuvant expands the breadth and duration of protection of mRNA-based vaccinesNature ImmunologyMay 22, 2026Kautilya K. Jena, Pengxiang Qu, Lauren Baracco, Shahab Saghaei, Keerti, Zahra Allahyari, Daniel Boehmer, Moriah Mitchell, Carly A. Dillen, Hai-Yun Li, et al.two washing steps in FACS buffer. For fixation, cells were incubated in fixation buffer (BioLegend, 420801) for 10 minutes at room temperature, followed by a washing step in FACS buffer. Samples were acquired

(18) Evaluation of Safety and Immunogenicity of High-Dose Quadrivalent Seasonal Influenza Split Vaccine: A Preclinical StudyVaccines (Basel)May 17, 2026Lanxin Jia, Ran Qiu, Jing Liu, Bo Liu, Xuanxuan Nian, Yang Le, Xixin Han, Qingmei Zhang, Xuedan Li, Zheng Gong, et al.min). Each sample tube received 500 μL of Fixation Buffer (BioLegend, San Diego, CA, USA; Cat. No. 420801) to fix the cells, which were incubated at room temperature in the dark for 20 min. After fixation,

(19) Activation-gated, T cell-restricted silencing of Dapk1 enhances Bacille Calmette-Guérin-elicited protective CD4+ memoryiScienceMay 15, 2026Zixuan Liu, Weihuang Liu, Chenyu Zhao, Xuanchang Bai, Fangting Zhou, Hang Sun, Jiahe Shi, Pinru Chen, Min Liu, Qin Pansubstrate,Biosharp,Cat# BL523B Zombie NIRTM Fixable Viability Kit,Biolegend,Cat# 423105 Fixation Buffer,Biolegend,Cat# 420801 Intracellular Staining Perm Wash Buffer(10×),Biolegend,Cat# 421002 Brefeldin A,Absin,Cat# abs810012 Cell lysis buffer

(20) Proteostasis sustains T cell differentiation potential and tumor-infiltrating lymphocyte functionCellMay 14, 2026Nicole E. Scharping, Xuezhen Ge, Maria Inês Matias, Fulin Jiang, Allison Cafferata, Maximilian Heeg, Alexander Monell, Giovanni Galletti, Kitty P. Cheung, Angelica Rock, et al.magnesium",Corning,Cat#20-021-CV "1,4-Dithioerythritol ≥99.0%",Sigma-Aldrich,Cat# D8255-25G Fixation Buffer,BioLegend,Cat#420801 eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set,Invitrogen,Cat#00-5523-00 BD Cytofix/Cytoperm™

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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