Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC,ICC
Quantity100ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(61) Recombinant human adenovirus type 5 synergizes with anti-PD-L1 antibody to promote anti-hepatocellular carcinoma effects through multilevel remodeling of the immune microenvironmentFrontiers in ImmunologyMarch 24, 2026Qiu Zhao, Min Xiao, Jian Ma, Xiaoqian Li, Cong Fu, Wenjing Ji, Lingna Ni, Jialin Fan, Qianqian Gao, Yanzhi BiAPC-CD86 (BioLegend, 105011), Alexa Fluor® 700-CD206 (BioLegend, 141733), fixation buffer (BioLegend, 420801), and intracellular staining permeabilization wash buffer (BioLegend, 421002). 2.7 HE, immunohistochemistry The

(62) Peripheral immune landscape in pancreatic ductal adenocarcinoma reveals expansion of effector states with disease progressioniScienceMarch 20, 2026Chen-Hsuan Yang, Yun-Yi Lung, Chieh-Chang Chen, I-Hsuan Lo, Chia-Jung Lien, Wei-Kai Wu, Ming-Shiang Wu, Wei-Chih Liao, Jian-Da Linstain,NanoEnTek,Cat# EBT-001 Brilliant Stain Buffer,BD Biosciences,Cat# 566385 Fixation Buffer,Biolegend,Cat# 420801 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, No critical

(63) Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicronJ ImmunolMarch 18, 2026Robert W P Glowacki, Jessica M Till, Orion D Brock, Vladimir Makarov, Morgan J Engelhart, Philip P Ahernby pipetting in 100 µl of a commercially available formaldehyde-based fixative (BioLegend, Cat. no. 420801; used when no intracellular staining was subsequently performed) or (ThermoFisher, Cat. # 00-5123-43

(64) Comprehensive single-cell metabolic profiling identifies targets to sensitize triple-negative breast cancer to chemo-immunotherapyCell Rep MedMarch 17, 2026Ying Xu, Xin-Yi Liu, Hang Zhang, Li Chen, Han Wang, Zhi-Ming Shao, Yi Xiao, Yi-Zhou Jiang420302 Intracellular Staining Permeabilization Wash Buffer (10x),BioLegend,Cat# 421002 Fixation Buffer,BioLegend,Cat# 420801 Cyto-Last™ Buffer,BioLegend,Cat# 422501 Cell Staining Buffer,BioLegend,Cat# 420201 Opal Polaris 7-Color

(65) Symbiotic exclusivity between CLOCK and TFPI2 drives stemness and immunosuppression in glioblastoma modelsJ Clin InvestMarch 17, 2026Fei Zhou, Lizhi Pang, Yang Liu, Fatima Khan, Peiwen Chen(BioLegend, #505850). After washing with FACS buffer, cells were incubated with fixation buffer (BioLegend, #420801). Then the labeled cells were analyzed using BD FACSymphony flow cytometer and FlowJo v10.8.1 software. Proliferation

(66) Selenium-loaded sustained-release schizophyllan alleviates pancreatic and pulmonary inflammatory damage in type 1 diabetes mellitus by modulating gut microbiota and T cell balanceJournal of NanobiotechnologyMarch 14, 2026Junming Luo, Siying Pei, Zhiying Ai, Runqing Rao, Zhuoyuan Yang, Xin Guo, Zeyi Ai, Lilin Fan, Fang ZouIntracellular staining was performed after permeabilization with fixation/permeabilization concentrate (420801, BioLegend, USA) mixed with diluent at a 1:3 ratio, followed by treatment with permeabilization buffer

(67) Prolonged Wnt3a exposure tolerizes macrophages to inflammatory stimuliFront ImmunolMarch 12, 2026Megan L. Tigue, Rincon Jagarlamudi, Channing Chi, Diana Diaz, Hua-Chang Chen, Quanhu Sheng, Sheau-Chiann Chen, Anna Schwarzkopf, Jeremy A. Goettel, Heather H. Pua, et al.temperature in the dark. After surface staining, cells were fixed with Fixation buffer (BioLegend, 420801) for 20 minutes in the dark and resuspended in 1X Intracellular Staining Permeabilization Wash Buffer

(68) Site-specific profiling of structure and function of Igµ B cell receptor glycansNature CommunicationsMarch 5, 2026M. D. Holborough-Kerkvliet, L. Hafkenscheid, S. Kroos, R. Biersteker, R. van de Wetering, O. Singh, E. Fadda, R. T. N. Tjokrodirijo, P. A. van Veelen, M. Wuhrer, et al.F(ab’)2 (Southern Biotech, #2062-01 and #2072-01, respectively) at 37 ⁰C. Fixation buffer (BioLegend, #420801) was used to fix the cells and was added immediately after the stimulation time passed. Cells were washed

(69) NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumorsNature CommunicationsFebruary 27, 2026Dominik Humer, Victoria Klepsch, Dietmar Rieder, Isabel Hölzl, Daniel Schreiber, Viktor Lang, Jiří Koutník, Sebastian Peer, Tajana Sajinovic, Viana Wille, et al.surface antibodies as stated above. Thereafter cells were fixed using the fixation buffer from Biolegend (420801) for 20 minutes at RT. After fixation, cells were permeabilized by washing twice with the kit’s Perm/Wash

(70) Investigating the Immune Effects of Radiotherapy in Non-Small Cell Lung Cancer—Results of the PD-RAD StudyOncology ResearchFebruary 24, 2026Shuhui Cheng, Tiana Kordbacheh, Antonia Banyard, Anshuman Chaturvedi, Diego Sanchez Martinez, Crispin T. Hiley, Maggie Harris, Clara Chan, Corinne Faivre-Finn, Timothy M. Illidge, et al.were washed in CSB and fixed in 1 mL of 4% paraformaldehyde (PFA) in PBS (fixation buffer, Biolegend, 420801) per 3 × 106 cells, for a minimum of 18 h. Finally, per 3 × 106 cells, 1 µL of iridium solution (10

(71) Long-acting interleukin-7 improves the efficacy of oncolytic viral therapy in glioblastomaNature CommunicationsFebruary 17, 2026Yuping Derek Li, David A. Giles, Yi Huang, Ashwani Kesarwani, Tong Hu, Logan Page, Amanda de Andrade Costa, Jingqin Luo, Alexandra Wolfarth, Donghoon Choi, et al.controls were used for setting population gates. For all staining procedures, cells were fixed (Biolegend, 420801), and permeabilization (Biolegend, 421002) was performed if intracellular staining was necessary. Absolute

(72) Fluorescence Intensity of Protein Tags Is Dependent on Their Subcellular LocationCellsFebruary 13, 2026Yan Chen, John P. Eichorst, Barbara Barylko, Joachim D. Mueller, Joseph P. AlbanesiSoftware, Broomfield, CO, USA). 2.3. Imaging All the cells were fixed with buffer from BioLegend (cat #420801; BioLegend, San Diego, CA, USA) for one minute before being exchanged to PBS for epifluorescence imaging.

(73) Reversible control of T cell exhaustion by NR4A transcription factors revealed through targeted protein degradationbioRxivFebruary 4, 2026Erik Ehinger, Kristy C Perez, Emi Sanchez, Bruno Villalobos Villalobos Reveles, Barsha Dash, Leo Josue Arteaga-Vazquez, Eric Johnson, Anjana Rao, Patrick G Hogan(Santa Cruz sc-393902), anti-mouse Nur77 AF488 (eBioscence 53–5965-82), Fixation Buffer (Biolegend 420801, eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (ThermoFisher 00–5523-00). Mouse T cell

(74) Endogenous CD28 drives the persistent activity of CAR T cells in myeloma and lymphoma modelsBlood Cancer DiscoveryFebruary 2, 2026Mackenzie M. Lieberman, Jason H. Tong, Nkechi U. Odukwe, Colin A. Chavel, Gina G. Bishara, Kimberly M. Crasti, Megan M. Herr, Payal Goala, Terence J. Purdon, Rebecca Burchett, et al.following fixation and permeabilization according to manufacturer’s instructions (BioLegend, catalog #420801 & #421002). Antibodies used in phenotypic analysis are included in the Key Resources Table and gating

(75) Investigation of the activity of phosphothioate and phosphothioate-LNA-modified oligonucleotides against HIV-1Front Med (Lausanne)February 2, 2026Ludmila Gotfrid, Kirill Elfimov, Maria Gashnikova, Aleksey Totmenin, Aleksandr Agaphonov, Natalya Gashnikovafixation was performed using Fixation Buffer (BioLegend, San Diego, California, United States; Cat. No. 420801), and cell permeabilization was carried out with Intracellular Staining Perm Wash Buffer (10X) (BioLegend,

(76) TBKBP1 induces capecitabine resistance through negative regulation of type I interferon pathway in triple-negative breast cancerOncogeneJanuary 28, 2026Wen-Ya Wu, Yun-Song Yang, Lisa Andriani, Yi-Fan Xie, Gen-Hong Di, Zhi-Ming Shao, Jun-Jie LiCocktail (BD Biosciences, #550583) for 5 h at 37 °C and permeabilized with Fixation Buffer (BioLegend, #420801) and Intracellular Staining Permeabilization Wash Buffer (BioLegend, #421002) according to the manufacturers’

(77) CAR-T manufacturing reduces heterogeneity between CIDP and multiple myeloma patient-derived T cellsTheranosticsJanuary 22, 2026Xu Wang, Pu Wang, Qi Zhou, Xianzheng Wei, Yuhang Jin, Ying Liao, Xuan Zhao, Rui Hou, Sijin Li, Zhangchun Guan, et al.additional 4 h. Finally, cells were processed using a fixation/permeabilization kit (BioLegend, cat#420801) before intracellular staining. Antibody panels: T cell subsets: CD45-PE (BioLegend, clone HI30, cat#304008),

(78) Amphiphilic Polymeric Micelle Spherical Nucleic Acids (SNAs) as Drug Delivery VehiclesBioconjugate ChemistryJanuary 21, 2026John P. Cavaliere, Connor M. Forsyth, Allen X. Guo, Janice Kang, Kathleen H. Ngo, Chad A. Mirkin#117329; BMDCs only)] for 15 min at 4 °C, washed, centrifuged, and fixed in BioLegend fixation buffer (#420801) for 30 min at 4 °C. After a final PBS wash and resuspension, samples were analyzed by flow cytometry

(79) 5-HT reuptake blockade induces pyroptosis in BRAFV600E-mutated melanomas via remodeling histone serotonylationCell Reports MedicineJanuary 20, 2026Aicun Li, Shoujia Xu, Jiachen Fan, Jingwei Liu, Xiaoyu Song, Liu Cao, Zhicheng Gong, Xiao Lufor 5 h at 37°C in 5% CO2. Following stimulation, cells were Fixed with Fixation Buffer (BioLegend, #420801) for 20 min at room temperature. Permeabilized using Intracellular Staining Permeabilization Wash Buffer

(80) Sialylation-immune-related lncRNA LINC01605 promotes tumor-infiltrating CD8+ T cell exhaustion and malignancy of clear cell renal cell carcinomaFrontiers in ImmunologyJanuary 16, 2026Ziran Dai, Hao Zhou, Zihao Feng, Mingxiao Zhang, Zheyu Ai, Gaowei Huang, Junjie Cen, Yanping Liang, Jinhuan Wei, Wei Chen, et al.PD-1 and LAG-3 for 20 min, on ice. The single-cell suspensions were then treated with Fixation Buffer (420801, BioLegend) and Intracellular Staining Perm Wash Buffer (421002, BioLegend) to stain intracellular markers

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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