Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(61) Fluorescence Intensity of Protein Tags Is Dependent on Their Subcellular LocationCellsFebruary 13, 2026Yan Chen, John P. Eichorst, Barbara Barylko, Joachim D. Mueller, Joseph P. AlbanesiSoftware, Broomfield, CO, USA). 2.3. Imaging All the cells were fixed with buffer from BioLegend (cat #420801; BioLegend, San Diego, CA, USA) for one minute before being exchanged to PBS for epifluorescence imaging.

(62) Reversible control of T cell exhaustion by NR4A transcription factors revealed through targeted protein degradationbioRxivFebruary 4, 2026Erik Ehinger, Kristy C Perez, Emi Sanchez, Bruno Villalobos Villalobos Reveles, Barsha Dash, Leo Josue Arteaga-Vazquez, Eric Johnson, Anjana Rao, Patrick G Hogan(Santa Cruz sc-393902), anti-mouse Nur77 AF488 (eBioscence 53–5965-82), Fixation Buffer (Biolegend 420801, eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (ThermoFisher 00–5523-00). Mouse T cell

(63) Endogenous CD28 drives the persistent activity of CAR T cells in myeloma and lymphoma modelsBlood Cancer DiscoveryFebruary 2, 2026Mackenzie M. Lieberman, Jason H. Tong, Nkechi U. Odukwe, Colin A. Chavel, Gina G. Bishara, Kimberly M. Crasti, Megan M. Herr, Payal Goala, Terence J. Purdon, Rebecca Burchett, et al.following fixation and permeabilization according to manufacturer’s instructions (BioLegend, catalog #420801 & #421002). Antibodies used in phenotypic analysis are included in the Key Resources Table and gating

(64) Investigation of the activity of phosphothioate and phosphothioate-LNA-modified oligonucleotides against HIV-1Front Med (Lausanne)February 2, 2026Ludmila Gotfrid, Kirill Elfimov, Maria Gashnikova, Aleksey Totmenin, Aleksandr Agaphonov, Natalya Gashnikovafixation was performed using Fixation Buffer (BioLegend, San Diego, California, United States; Cat. No. 420801), and cell permeabilization was carried out with Intracellular Staining Perm Wash Buffer (10X) (BioLegend,

(65) TBKBP1 induces capecitabine resistance through negative regulation of type I interferon pathway in triple-negative breast cancerOncogeneJanuary 28, 2026Wen-Ya Wu, Yun-Song Yang, Lisa Andriani, Yi-Fan Xie, Gen-Hong Di, Zhi-Ming Shao, Jun-Jie LiCocktail (BD Biosciences, #550583) for 5 h at 37 °C and permeabilized with Fixation Buffer (BioLegend, #420801) and Intracellular Staining Permeabilization Wash Buffer (BioLegend, #421002) according to the manufacturers’

(66) CAR-T manufacturing reduces heterogeneity between CIDP and multiple myeloma patient-derived T cellsTheranosticsJanuary 22, 2026Xu Wang, Pu Wang, Qi Zhou, Xianzheng Wei, Yuhang Jin, Ying Liao, Xuan Zhao, Rui Hou, Sijin Li, Zhangchun Guan, et al.additional 4 h. Finally, cells were processed using a fixation/permeabilization kit (BioLegend, cat#420801) before intracellular staining. Antibody panels: T cell subsets: CD45-PE (BioLegend, clone HI30, cat#304008),

(67) Amphiphilic Polymeric Micelle Spherical Nucleic Acids (SNAs) as Drug Delivery VehiclesBioconjugate ChemistryJanuary 21, 2026John P. Cavaliere, Connor M. Forsyth, Allen X. Guo, Janice Kang, Kathleen H. Ngo, Chad A. Mirkin#117329; BMDCs only)] for 15 min at 4 °C, washed, centrifuged, and fixed in BioLegend fixation buffer (#420801) for 30 min at 4 °C. After a final PBS wash and resuspension, samples were analyzed by flow cytometry

(68) 5-HT reuptake blockade induces pyroptosis in BRAFV600E-mutated melanomas via remodeling histone serotonylationCell Reports MedicineJanuary 20, 2026Aicun Li, Shoujia Xu, Jiachen Fan, Jingwei Liu, Xiaoyu Song, Liu Cao, Zhicheng Gong, Xiao Lufor 5 h at 37°C in 5% CO2. Following stimulation, cells were Fixed with Fixation Buffer (BioLegend, #420801) for 20 min at room temperature. Permeabilized using Intracellular Staining Permeabilization Wash Buffer

(69) Sialylation-immune-related lncRNA LINC01605 promotes tumor-infiltrating CD8+ T cell exhaustion and malignancy of clear cell renal cell carcinomaFrontiers in ImmunologyJanuary 16, 2026Ziran Dai, Hao Zhou, Zihao Feng, Mingxiao Zhang, Zheyu Ai, Gaowei Huang, Junjie Cen, Yanping Liang, Jinhuan Wei, Wei Chen, et al.PD-1 and LAG-3 for 20 min, on ice. The single-cell suspensions were then treated with Fixation Buffer (420801, BioLegend) and Intracellular Staining Perm Wash Buffer (421002, BioLegend) to stain intracellular markers

(70) Inhibiting Glycan Degradation Prevents HIV-Induced Inflammaging and Cognitive ImpairmentbioRxivJanuary 16, 2026Leila B. Giron, Alejandra Borjabad, Eran Hadas, Janeway Granche, Erika G. Marques de Menezes, Thomas A. Premeaux, Hongxia He, Stephen T. Yeung, Shalini Singh, Courtney Friday, et al.After incubation, cells were washed with PBS-2%FBS, fixed in PFA (Fixation buffer, Biolegend; Catalog# 420801), and analyzed by flow cytometry (BD Biosciences LSRII). Cytokines were measured in the supernatant

(71) Serial Expression of Pro-Inflammatory Biomarkers in Acute Lung Injury During the Post-Resuscitation Periods in Rats with Cardiac ArrestInternational Journal of Molecular SciencesJanuary 13, 2026Han-Ping Wu, Kuan-Miao Lin, Mao-Jen Lin(202103, Biolegend). To detect the intracellular marker Foxp3, samples were treated with fixation buffer (420801, BioLegend) and permeabilization buffer (421002, BioLegend) and then stained with PE anti-mouse/rat/human FOXP3

(72) TDP-43 dysfunction leads to the accumulation of cryptic transposable element-derived exons, crypTEs, in iPSC derived neurons and ALS/FTD patient tissuesbioRxivJanuary 13, 2026Isobel Bolger, Regina Shaw, Oliver H Tam, Cláudio Gouveia Roque, Christopher A Jackson, Kathryn O’Neill, NYGC ALS Consortium, Colin Smith, Hemali Phatnani, Karthick Natarajan, et al.μ-Slide 4-Well chamber slides (ibidi, Cat. no. 80426), fixed with Fixation Buffer (BioLegend, Cat. no. 420801) for 15 minutes at room temperature, washed with PBS (no calcium and magnesium), and blocked with Intercept®

(73) Butyrophilin 2A2 promotes T cell immunoregulation via CD45 phosphatase activation and protects against murine autoimmune glomerulonephritis and pregnancy lossNature communicationsJanuary 8, 2026Ali S, Berg AH, Yamashita M, Covarrubias AE, Mundell J, Shah PN, Zhang R, Dupont V, Shin BH, Yang S, et al.were stained with surface antibodies for 25 min at RT, washed and then fixed with Fixation Buffer (420801; Biolegend) for 20 min at RT and washed with 1X Intracellular Staining Permeabilization Wash Buffer

(74) Single‐Nucleus Multi‐Omics Reveals Hypoxia‐Driven Angiogenic Programs and Their Epigenetic Control in Sinonasal Squamous Cell CarcinomaAdvanced ScienceJanuary 7, 2026Chaelin You, Jaewoo Park, Jung Yeon Jang, Junho Noh, Jaehyun Lee, Geunho Kwon, Myeong Sang Yu, Yoo‐Sam Chung, Seung‐Jun Lee, Keunsoo Kang, et al.Catalog no. 12604013). Cells were fixed using pre‐warmed (37 °C) fixation buffer (BioLegend, Catalog no. 420801) at 37 °C for 15 min. Following washing, permeabilization was carried out using pre‐chilled (−20 °C)

(75) Suxiao Jiuxin Pill Promotes Post-Myocardial Infarction Cardiac Repair by Enhancing Regulatory T Cell Function via the ST2L Signaling PathwayJournal of Inflammation ResearchJanuary 6, 2026Lusha Zhang, Ying Guo, Yuming Fan, Ting Liu, Tianyu Wang, Mengyao Li, Shaoxia Wang, Qi Yu, Hong Wang(506915, Biolegend) was performed using the Cell Activation Cocktail (423304, BioLegend). Fixation (420801, BioLegend) and Intracellular Staining Permeabilization Wash Buffer (10X) (421002, BioLegend) were used

(76) Suxiao Jiuxin Pill Promotes Post-Myocardial Infarction Cardiac Repair by Enhancing Regulatory T Cell Function via the ST2L Signaling PathwayJ Inflamm ResJanuary 6, 2026Lusha Zhang, Ying Guo, Yuming Fan, Ting Liu, Tianyu Wang, Mengyao Li, Shaoxia Wang, Qi Yu, Hong Wang(506915, Biolegend) was performed using the Cell Activation Cocktail (423304, BioLegend). Fixation (420801, BioLegend) and Intracellular Staining Permeabilization Wash Buffer (10X) (421002, BioLegend) were used

(77) Adaptive-like CAR-iPSC-CD4⁺ T cells outperform CD8⁺ counterparts in sustained ALL controlInflammation and RegenerationJanuary 3, 2026Qingyi Guo, Chaoqi Zhang, Bo Wang, Shoichi Iriguchi, Akihiro Ishikawa, Atsutaka Minagawa, Tomoko Ishii, Yohei Kawai, Shin Kanekoon ice for 30 min. Intracellular staining was carried out using Fixation Buffer (BioLegend, Cat. No. 420801) and Intracellular Staining Perm Wash Buffer (BioLegend, Cat. No. 421002), following the manufacturer’s

(78) Microglial TBK1 Signaling Promotes Breast Cancer Brain MetastasisCancer ResearchJanuary 2, 2026Fatima Khan, Edgar Petrosyan, Yang Liu, Atousa Bahrami, Erfan Taefi, Changiz Geula, Maciej S. Lesniak, Peiwen Chenanalysis. For intracellular detection of P-TBK1, cells were fixed with fixation buffer (BioLegend, #420801), permeabilized with Perm buffer, and stained with P-TBK1 antibody (CST, #5483S, RRID:AB_10693472),

(79) Microglial TBK1 Signaling Promotes Breast Cancer Brain MetastasisCancer ResJanuary 2, 2026Fatima Khan, Edgar Petrosyan, Yang Liu, Atousa Bahrami, Erfan Taefi, Changiz Geula, Maciej S Lesniak, Peiwen Chenanalysis. For intracellular detection of P-TBK1, cells were fixed with fixation buffer (BioLegend, #420801), permeabilized with Perm buffer, and stained with P-TBK1 antibody (CST, #5483S, RRID:AB_10693472),

(80) Tubule-derived CCN1 drives renal repair via α(v)β(5)-STAT6-ARG1-dependent reprogramming of macrophagesCell death & diseaseDecember 21, 2025Zhang N, Li C, Xin Y, Song Z, Li T, Zhao R, Gu M, Xu L, Wang Y, Man X, et al.F4/80, CD86, CCR2, and Ly6C for 15 minutes in the dark. Cells were fixed with 250 μL fixation buffer (#420801, Biolegend, San Diego, CA, USA), permeabilized with intracellular permeabilization buffer (#421002,

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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