Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(101) Ginsenoside Rh1 inhibits tumor growth in mice with colorectal cancer and depressive symptoms via modulation of the gut microbiota and tumor microenvironmentMol Med RepOctober 8, 2025Tingting Dai, Lingchang Li, Jiaxin Li, Xiaobin Tan, Yi Luo, Xuedong Wang, Jie Song, Sujuan Li, Songshan Shipopulation. For intracellular staining, cells were fixed with fixation buffer (4% paraformaldehyde, cat. no. 420801; BioLegend, Inc.) in the dark for 30 min at room temperature and washed with permeabilization buffer

(102) Identification of strain-specific cues that regulate biofilm formation in Bacteroides thetaiotaomicronMicrobiology SpectrumOctober 7, 2025Robert W.P. Glowacki, Morgan J. Engelhart, Jessica M. Till, Anagha Kadam, Ina Nemet, Naseer Sangwan, Philip P. Ahernremoved, and the dishes were washed three times with PBS. A total of 500 µL of fixative (Biolegend, Cat. # 420801) was added to dishes and incubated for 1 hour at room temperature in the dark. The fixative was removed,

(103) Immunodominant T cell responses to SARS-CoV-2 nucleocapsid protein in Omicron breakthrough infection post-inactivated vaccinationVirulenceOctober 6, 2025Jie Ning, Yue Xu, Yayi Ren, Zelin Zhang, Ying Chen, Xianhuang Zeng, Jianquan Zhang, Chao Wuanti-CD4-AF700. After another round of wash, the cells were fixed using Fixation buffer (BioLegend, USA, Cat#420801) for 20 mins at 4°C, and then permeabilized using Intracellular Staining Perm Wash Buffer (BioLegend,

(104) Platelet activation plays a pro-inflammatory role in myasthenia gravisNat CommunOctober 2, 2025Qi Wen, Shu Zhang, Yaye Wang, Haoran Liu, Jingsi Wang, Shengyao Su, Nairong Xie, Yan Lu, Li Di, Min Xu, et al.For intracellular cytokine staining, the cells were then treated with Fix/Perm solution (Biolegend, 420801) and re-stained with PE/Cyanine7-labeled anti-IFNγ (1:100 dilution; Biolegend, 502527), PE-labeled TNFα

(105) Norcantharidin promotes M1 macrophage polarization and suppresses colorectal cancer growthActa Pharmacologica SinicaOctober 1, 2025Xiao-man Wei, Si-cheng Lu, Liu Li, Ying-jie Gao, Jun-yi Wang, Song-yang Xi, Ling-yu Linda Ye, Wei-xing Shen, Mian-hua Wu, Dayue Darrel Duan, et al.after cell surface antibody staining, followed by cell fixation using Fixation Buffer (BioLegend, #420801) for 30 min and permeabilization using Intracellular Staining PermeabilizationWash Buffer (BioLegend,

(106) Coupling CRISPR scanning with targeted chromatin accessibility profiling using a double-stranded DNA deaminaseNature MethodsOctober 1, 2025Heejin Roh, Simon P. Shen, Yan Hu, Hui Si Kwok, Allison P. Siegenfeld, Ceejay Lee, Marcanthony U. Zepeda, Chun-Jie Guo, Shelby A. Roseman, Caroline Comenho, et al.Cat#349116). For intracellular staining of HbF, cells were washed, fixed with fixation buffer (Biolegend, 420801) and permeabilized with intracellular staining permeabilization wash buffer (Biolegend, 421002). Cells

(107) Matrix-Guided Vascular-like Cord Formation by MRC-5 Lung Fibroblasts: Evidence of Structural and Transcriptional PlasticityCellsSeptember 29, 2025Nikoleta F. Theodoroula, Alexandros Giannopoulos-Dimitriou, Aikaterini Saiti, Aliki Papadimitriou-Tsantarliotou, Androulla N. Miliotou, Giannis Vatsellas, Yiannis Sarigiannis, Eleftheria Galatou, Christos Petrou, Dimitrios G. Fatouros, et al.were washed twice with PBS and fixed in Intracellular Staining Fixation Buffer (BioLegend, Cat. No. 420801) for 20 min. The cells were then rinsed with PBS and permeabilized with Intracellular Staining Permeabilization

(108) An Association Between GLP-1 Receptor Expression on Regulatory T Cells and the Severity of Coronary Artery Stenosis and Inflammatory Dysregulation in Coronary Heart DiseaseRev Cardiovasc MedSeptember 25, 2025Mingzhu Lv, Mengmeng Yue, Min Yang, Xiaolei Li, Kun Wu, Ting Yao, Hang Qian, Long Chen, Wenwen Wu, Xinwen Min, et al.cells were fixed/permeabilized using the FoxP3/Transcription Factor Staining Buffer Set (BioLegend, #420801) and stained with Alexa Fluor 647-conjugated anti-FoxP3 (Clone 236A/E7, BioLegend, #320214) for 45 min

(109) An Association Between GLP-1 Receptor Expression on Regulatory T Cells and the Severity of Coronary Artery Stenosis and Inflammatory Dysregulation in Coronary Heart DiseaseRev Cardiovasc MedSeptember 25, 2025Mingzhu Lv, Mengmeng Yue, Min Yang, Xiaolei Li, Kun Wu, Ting Yao, Hang Qian, Long Chen, Wenwen Wu, Xinwen Min, et al.cells were fixed/permeabilized using the FoxP3/Transcription Factor Staining Buffer Set (BioLegend, #420801) and stained with Alexa Fluor 647-conjugated anti-FoxP3 (Clone 236A/E7, BioLegend, #320214) for 45 min

(110) Optimization of the Blocking and Signal Preservation Protocol in High‐Parameter Flow CytometryCurrent ProtocolsSeptember 25, 2025Oliver T. Burton, James Dooley, Adrian Listonsolution (BioLegend, cat No. 420601; various sources are acceptable)Fixation Buffer (BioLegend, cat. no. 420801), Cyto‐Fast Fix/Perm Buffer Set (BioLegend, cat. no. 426803) or IC Fixation Buffer (Thermo Fisher, cat.

(111) Engineered T cells stimulate dendritic cell recruitment and antigen spreading for potent anti-tumor immunityCell Reports MedicineSeptember 16, 2025Zhen Xiao, Jiajia Wang, Shidian He, Lin Wang, Jingxing Yang, Wenhui Li, Kaili Ma, Yabo Zhou, Xiaowei Liu, Shiyou Wang, et al.System,Vazyme,Cat#DD1204-01 Chromium Single Cell 30 v2 reagent kit,10× Genomics,N/A Fixation Buffer,BioLegend,Cat#420801 Intracellular Staining Permeabilization Wash Buffer,BioLegend,Cat#421002 eBioscience™ Permeabilization

(112) Surface-Engineered HA-PEG-ICG/PLGA Nanoprobes with Vessels Targeting for Lymphatic System VisualizationACS Applied Bio MaterialsSeptember 15, 2025Hao-Han Chiang, Yu-Teng Chang, Wei-Ren Huang, Min-Xuan Cai, Chin-Hsing Feng, Jia-Ning Syu, Chih-Sheng Lai, Yi-Hsin ChienFixation was performed in the dark for 20 min at room temperature using a fixation buffer (BioLegend, Cat# 420801). To permeabilize the cells, 0.1% Triton X-100 in PBS was applied for 3–5 min, followed by 2–3 washes

(113) Epigenetic silencing of interleukin-10 by host-derived oxidized phospholipids supports a lethal inflammatory response to infectionsImmunitySeptember 9, 2025Marco Di Gioia, Valentina Poli, Piao J. Tan, Roberto Spreafico, Anne Chu, Alex G. Cuenca, Zhongyang Wu, Mehdi Benamar, Laura Pandolfi, Federica Meloni, et al.107622) and BV785 CD11c (BioLegend, Cat# 117336). Cells were fixed (Fixation buffer, BioLeged, Cat# 420801), permeabilized (Intracellular Staining Permeabilization Wash Buffer, BioLegend, Cat# 421002) and incubated

(114) Bronchial pyroptosis promotes Th17 inflammation in steroid-insensitive asthma mouseInnate ImmunAugust 28, 2025Yun Lin, Jianhua Yin, Xia Yang, Jianghong Wei, Yaxi Liang, Chengfeng Zhou, Dongfang Zou, Shuyuan Chucocktail (00-4975-93, eBioscience, CA, USA) at 37°C for 5 h, and then incubated with IC fixation buffer (420801, Biolegend) for 30 min without light according to manufacturer's protocol. The cells were stained with

(115) The bispecific innate cell engager AFM28 eliminates CD123(+) leukemic stem and progenitor cells in AML and MDSNature communicationsAugust 21, 2025Schmitt N, Siegler JJ, Beck A, Müller T, Kozlowska I, Sarlang S, Reusch U, Knackmuss S, Medina-Echeverz J, Koch J, et al.non-targeting RSV/CD16A, followed by fixation and cell lysis (Fixation buffer, BioLegend, cat. no. 420801; True-Phos Perm Buffer, BioLegend, cat. no. 425401). Analyses were performed using BD FACSCelesta (Becton Dickinson

(116) Isolation and Ex Vivo Testing of CD8+ T-Cell Division and Activation Using Mouse SplenocytesBio ProtocAugust 20, 2025Melissa Dolan, Yuhao Shi, Amber McKenery, Stephanie Tzetzo, Kohei Chida, Kazuaki Takabe, Scott I. Abrams, John M.L. Ebos1) 13. Flow cytometry staining antibodies (see Table 1) 14. Fixation buffer (BioLegend, catalog number: 420801) 15. Intracellular staining permeabilization wash buffer (10×) (BioLegend, catalog number: 421002) 16.

(117) Isolation and Ex Vivo Testing of CD8+ T-Cell Division and Activation Using Mouse SplenocytesBio ProtocAugust 20, 2025Melissa Dolan, Yuhao Shi, Amber McKenery, Stephanie Tzetzo, Kohei Chida, Kazuaki Takabe, Scott I. Abrams, John M.L. Ebos1) 13. Flow cytometry staining antibodies (see Table 1) 14. Fixation buffer (BioLegend, catalog number: 420801) 15. Intracellular staining permeabilization wash buffer (10×) (BioLegend, catalog number: 421002) 16.

(118) Rewired type I IFN signaling is linked to age-dependent differences in COVID-19Cell Reports MedicineAugust 19, 2025Lev Petrov, Sophia Brumhard, Sebastian Wisniewski, Philipp Georg, David Hillus, Anna Hiller, Rosario Astaburuaga-García, Nils Blüthgen, Emanuel Wyler, Katrin Vogt, et al.(37°C) commercially available fixation buffer, according to manufacturer`s protocol (BioLegend Cat 420801). After 15 min of fixation at 37°C, cells were washed in FACS buffer (2 times, 500xg, 5 min, at room

(119) BRD9 inhibition overcomes oncolytic virus therapy resistance in glioblastomaCell Reports MedicineAugust 19, 2025Chen Guo, Zhilin Long, Peng Lin, Yinan Shen, Yiye Zhong, Jingjing Qian, Jichuan Yu, Weixi Zhao, Fuyi Liu, Yiling Ma, et al.staining at 4°C for 45 min and the intracellular staining was performed with fixation buffer (Biolegend, #420801) and intracellular staining buffer (Biolegend, #421002) according to the manufacturer’s instructions.

(120) Early-life human CD8 + T cells exhibit rapid, short-lived effector responses and a unique transcription factor landscapeProceedings of the National Academy of SciencesAugust 5, 2025Nina N. Brodsky, Monisha Chakder, Dinesh Babu Uthaya Kumar, Anis Barmada, Julia Wang, Weihong Gu, Oluwabunmi Olaloye, Anjali Ramaswamy, Aanchal Wats, Liza Konnikova, et al.albumin--BSA, 2 mL EDTA, 2mM, pH 8.5) and then fixed with 4% paraformaldehyde (BioLegend, catalog no. 420801). Cells were then permeabilized using perm/wash buffer (BioLegend, catalog no. 421002). Intracellular

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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