Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC,ICC
Quantity100ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(101) Long noncoding RNA LncBAR enhances BRG1 protein to promote cardiomyocyte cell cycle progression and cardiac repairnpj Regenerative MedicineNovember 17, 2025Jun Li, Huanhuan Cai, Yufan Chen, Ruiqi Pi, Lilin Xiang, Zhibing Lu, Yan Zhou, Li Wangby trypsin digestion at 37 °C for 3 min and fixed and permeabilized by fixation buffer (Biolegend, 420801), then incubated with antibodies listed in Supplementary Table 2 at RT for 30 min at concentrations

(102) Beclin1 deficiency unlocks cardiac lineage commitment through convergent Wnt and BMP signaling activationStem Cell Research & TherapyNovember 13, 2025Lilin Xiang, Zhenhua Jia, Zhangyi Yu, Lenan Wang, Huanhuan Cai, Tong Zhang, Zhibing Lu, Li Wangtrypsin digestion at 37 °C for 5 min. Cells were fixed and permeabilized with Fixation Buffer (Biolegend, 420801), then incubated with primary antibody against α-actinin and corresponding fluorescent secondary antibodies.

(103) Controlling intracellular processing to enhance spherical nucleic acid immune stimulationProceedings of the National Academy of SciencesNovember 11, 2025Janice Kang, Michelle H. Teplensky, Jasper W. Dittmar, Michael Evangelopoulos, Jeongmin Hwang, Chad A. MirkinPBS, centrifuged for 5 min at 1200 rpm, aspirated and fixed with 100 μL of fixation buffer (BioLegend, 420801) for 15 min at 4°C. The fixation buffer was washed by adding 600 μL of PBS, centrifuging at 1200 rpm

(104) A Semi-Automated Imaging Flow Cytometry Workflow for High-Throughput Quantification of Compound Internalization with IDEAS and FluoSta SoftwareMethods and ProtocolsNovember 9, 2025Kirill Elfimov, Ludmila Gotfrid, Alina Nokhova, Mariya Gashnikova, Dmitriy Baboshko, Aleksei Totmenin, Aleksandr Agaphonov, Natalya Gashnikova(Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. MA1-065-A647).Fixation Buffer (BioLegend, San Diego, CA, USA; Cat. No. 420801).Intracellular Staining Permeabilization Wash Buffer (BioLegend, San Diego, CA, USA; Cat. No. 421002).Cell Staining Buffer

(105) Myeloid ADAM17 induces ST2+ T regulatory cells that limit obesity-induced metabolic inflammation through changes in both membrane and soluble TNFBiomedicine & PharmacotherapyNovember 1, 2025Joseph C. Lownik, Matthew P. Zellner, Jared Farrar, Anuj Tharakan, Francesco S. Celi, Daniel H. Conrad, Rebecca K. MartinTable 1) for 45 min on ice. Cells were washed with FACS buffer and fixed in Fixation Buffer (BioLegend, 420801) for 10 min, or secondaries were added and incubated for 30 min on ice followed by washing and fixation.

(106) Immunomodulation of behavior impairment via spleen-specific targeting lipid nanoparticles in a MeCP2 transgenic mouse modelScientific ReportsOctober 31, 2025Shu Zhang, Yaxi Li, Bowen Yang, Aygun Teymur, Fatin Atrooz, Sheereen Majd, Chandra Mohan, Samina Salim, Tianfu Wusurface staining antibodies. Afterward, the cells were fixed with fixation buffer (Biolegend, catalog#420801), permeabilized using Permeabilization Wash Buffer (Biolegend, catalog#421002), and incubated with MeCP2

(107) Natural killer cells and IFN-γ protect against liver injury during HAV infection in miceJournal of VirologyOctober 23, 2025Ichiro Misumi, You Li, Takayoshi Shirasaki, Lixin Yang, Maryna Kapustina, Stanley M. Lemon, Jason K. Whitmireagainst Fc-receptors. For intracellular staining, cells were fixed with fixation buffer (BioLegend; #420801) and permeabilized using Permeabilization Wash buffer (BioLegend; #421002) and incubated with antibodies

(108) ACOX2 destabilizes the MRE11-RAD50-NBS1 complex and boosts anticancer immunity via the cGAS-STING pathway in clear cell renal cell carcinomaMol CancerOctober 21, 2025Shiqi Ye, Wenhao Xu, Zheqi Chen, Chuanying Zhu, Qintao Ge, Jiahe Lu, Kun Chang, Xi Tian, Aihetaimujiang Anwaier, Shuxuan Zhu, et al.suspension with cell staining buffer (Biolegend, 420201), fixation with fixation Buffer (Biolegend, 420801), permeabilization with intracellular staining permeabilization wash buffer (Biolegend, 421002). Then,

(109) M1-like macrophages regulate T cell infiltration in colorectal cancer through P2X4 receptoriScienceOctober 17, 2025Kun Zhou, Xintian Zhang, Yu Liang, Han Yao, Yichao Hou, Xingming Zhang, Leilei Du, Wenfeng Wang, Jianhua Wang, Xiangjun Meng(America),Cat# M8823 FLAG peptide,Sigma-Aldrich (America),Cat# F4799 Fixation Buffer,BioLegend (America),Cat# 420801 Permeabilization Wash Buffer,BioLegend (America),Cat# 421002 FastPure EndoFree Plasmid Maxi Kit,Vazyme

(110) Modulating Subcellular Localization to Preserve the Stability and Functionality of Intracellular NanobodiesAntibodies (Basel)October 16, 2025Wenli Sun, Keke Huang, Yaping Cheng, Ailing Huang, Yu Kong, Jun Lu, Tianlei Ying, Yanling WuFlow Cytometry Analysis Following transfection, harvested cells were fixed in fixation buffer (cat#420801, Biolegend, San Diego, CA, USA) at 37 °C for 20 min in the dark, then washed twice with Intracellular

(111) Mirabegron treatment reduces myofibroblasts and CXCR2 expression in adipose tissue in obesityMol MedOctober 14, 2025Brian S. Finlin, Hasiyet Memetimin, Philip M. Westgate, Jin Chen, Esther E. Dupont-Versteegden, Philip A. KernTGFβ2 as described above. After treatment, the cells were incubated with fixation buffer (BioLegend, 420801) for 20 min in the dark at room temperature, washed with PBS, blocked with staining buffer (#554656,

(112) Ginsenoside Rh1 inhibits tumor growth in mice with colorectal cancer and depressive symptoms via modulation of the gut microbiota and tumor microenvironmentMol Med RepOctober 8, 2025Tingting Dai, Lingchang Li, Jiaxin Li, Xiaobin Tan, Yi Luo, Xuedong Wang, Jie Song, Sujuan Li, Songshan Shipopulation. For intracellular staining, cells were fixed with fixation buffer (4% paraformaldehyde, cat. no. 420801; BioLegend, Inc.) in the dark for 30 min at room temperature and washed with permeabilization buffer

(113) Identification of strain-specific cues that regulate biofilm formation in Bacteroides thetaiotaomicronMicrobiology SpectrumOctober 7, 2025Robert W.P. Glowacki, Morgan J. Engelhart, Jessica M. Till, Anagha Kadam, Ina Nemet, Naseer Sangwan, Philip P. Ahernremoved, and the dishes were washed three times with PBS. A total of 500 µL of fixative (Biolegend, Cat. # 420801) was added to dishes and incubated for 1 hour at room temperature in the dark. The fixative was removed,

(114) Immunodominant T cell responses to SARS-CoV-2 nucleocapsid protein in Omicron breakthrough infection post-inactivated vaccinationVirulenceOctober 6, 2025Jie Ning, Yue Xu, Yayi Ren, Zelin Zhang, Ying Chen, Xianhuang Zeng, Jianquan Zhang, Chao Wuanti-CD4-AF700. After another round of wash, the cells were fixed using Fixation buffer (BioLegend, USA, Cat#420801) for 20 mins at 4°C, and then permeabilized using Intracellular Staining Perm Wash Buffer (BioLegend,

(115) Platelet activation plays a pro-inflammatory role in myasthenia gravisNat CommunOctober 2, 2025Qi Wen, Shu Zhang, Yaye Wang, Haoran Liu, Jingsi Wang, Shengyao Su, Nairong Xie, Yan Lu, Li Di, Min Xu, et al.For intracellular cytokine staining, the cells were then treated with Fix/Perm solution (Biolegend, 420801) and re-stained with PE/Cyanine7-labeled anti-IFNγ (1:100 dilution; Biolegend, 502527), PE-labeled TNFα

(116) Coupling CRISPR scanning with targeted chromatin accessibility profiling using a double-stranded DNA deaminaseNature MethodsOctober 1, 2025Heejin Roh, Simon P. Shen, Yan Hu, Hui Si Kwok, Allison P. Siegenfeld, Ceejay Lee, Marcanthony U. Zepeda, Chun-Jie Guo, Shelby A. Roseman, Caroline Comenho, et al.Cat#349116). For intracellular staining of HbF, cells were washed, fixed with fixation buffer (Biolegend, 420801) and permeabilized with intracellular staining permeabilization wash buffer (Biolegend, 421002). Cells

(117) Norcantharidin promotes M1 macrophage polarization and suppresses colorectal cancer growthActa Pharmacologica SinicaOctober 1, 2025Xiao-man Wei, Si-cheng Lu, Liu Li, Ying-jie Gao, Jun-yi Wang, Song-yang Xi, Ling-yu Linda Ye, Wei-xing Shen, Mian-hua Wu, Dayue Darrel Duan, et al.after cell surface antibody staining, followed by cell fixation using Fixation Buffer (BioLegend, #420801) for 30 min and permeabilization using Intracellular Staining PermeabilizationWash Buffer (BioLegend,

(118) Matrix-Guided Vascular-like Cord Formation by MRC-5 Lung Fibroblasts: Evidence of Structural and Transcriptional PlasticityCellsSeptember 29, 2025Nikoleta F. Theodoroula, Alexandros Giannopoulos-Dimitriou, Aikaterini Saiti, Aliki Papadimitriou-Tsantarliotou, Androulla N. Miliotou, Giannis Vatsellas, Yiannis Sarigiannis, Eleftheria Galatou, Christos Petrou, Dimitrios G. Fatouros, et al.were washed twice with PBS and fixed in Intracellular Staining Fixation Buffer (BioLegend, Cat. No. 420801) for 20 min. The cells were then rinsed with PBS and permeabilized with Intracellular Staining Permeabilization

(119) Optimization of the Blocking and Signal Preservation Protocol in High‐Parameter Flow CytometryCurrent ProtocolsSeptember 25, 2025Oliver T. Burton, James Dooley, Adrian Listonsolution (BioLegend, cat No. 420601; various sources are acceptable)Fixation Buffer (BioLegend, cat. no. 420801), Cyto‐Fast Fix/Perm Buffer Set (BioLegend, cat. no. 426803) or IC Fixation Buffer (Thermo Fisher, cat.

(120) An Association Between GLP-1 Receptor Expression on Regulatory T Cells and the Severity of Coronary Artery Stenosis and Inflammatory Dysregulation in Coronary Heart DiseaseRev Cardiovasc MedSeptember 25, 2025Mingzhu Lv, Mengmeng Yue, Min Yang, Xiaolei Li, Kun Wu, Ting Yao, Hang Qian, Long Chen, Wenwen Wu, Xinwen Min, et al.cells were fixed/permeabilized using the FoxP3/Transcription Factor Staining Buffer Set (BioLegend, #420801) and stained with Alexa Fluor 647-conjugated anti-FoxP3 (Clone 236A/E7, BioLegend, #320214) for 45 min

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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