Fixation Buffers

Fixation Buffers are reagents used to preserve biological samples, maintaining cellular and tissue architecture while stabilizing proteins for downstream applications like microscopy or flow cytometry. They work by chemically crosslinking proteins (e.g., formaldehyde-based fixatives) or precipitating them (e.g., alcohol-based fixatives) to immobilize structures and prevent degradation. Different formulations are tailored to specific applications, such as formalin for histology or paraformaldehyde for immunofluorescence. Some kits will also include permeabilization reagents, as fixation is not permissive to intracellular components. When choosing, consider compatibility with your sample type and subsequent procedures, such as antigen retrieval or staining. Evaluate buffer concentration, pH, and whether it's ready-to-use or requires preparation. Visit the supplier pages for more product specifications, protocols, and usage recommendations.