Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(41) A Microbial Lipid‐ATP Synthase Axis Fuels NK Cell Antitumor ActivityAdvanced ScienceApril 13, 2026Kaiyuan Yu, Xinyu Sun, Wanxia Ma, Jianming Yang, Xuan Sun, Lisong Zhang, Yumeng Liu, Tianshu Ren, Qi Wang, Jingyu Wang, et al.cells and NK cells for 30 min at 4°C. Cells were fixed for 30 min with fixation buffer (Biolegend, 420801), followed by permeabilization using an intracellular staining permeabilization wash buffer (Biolegend,

(42) Unveiling alternate pathways for SARS-CoV-2 infection via extracellular vesicle-mediated transfer of ACE2 and TMPRSS2Nature CommunicationsApril 10, 2026Martha Rea-Moreno, Lu Tian, Tara N. Tavakol, Min-Chi Yang, Nicole M. Pek, Shubham Gulati, Helena Bugacov, Cristina Cusmai, Gbalekan Dawodu, Remi V. Klotz, et al.EPD01-01) in PBS). For intracellular staining, the cells were fixed using the Fixation Buffer (BioLegend, 420801) and permeabilized with an Intracellular Staining Permeabilization Wash Buffer (BioLegend, 421002) according

(43) DDR1 Promotes Immune Evasion in Colorectal Cancer by Orchestrating IL33-Mediated M2-like Polarization of Tumor-Associated MacrophagesCancer Immunol ResApril 10, 2026Xiaofan Duan, Gaoshaer Yeerkenbieke, Yanjun Feng, Mingwang Zhou, Yumei Zhang, Jiuli Zhou, Jin Liminutes, ice). For intracellular staining, cells were washed with PBS, fixed using a fixation buffer (#420801, BioLegend) for 30 minutes, and stained with an anti–mouse CD206. For macrophage phenotyping, BMDMs

(44) A Rasa3-Gαi signaling axis orchestrates B lymphocyte trafficking into and through lymphoid organsCell reportsApril 9, 2026Park C, Hwang IY, Harrison K, Huang NN, Smelkinson M, Ganesan S, Kim HG, Schwartzberg PL, Kehrl JH.796-IC-050 "Recombinant Mouse MAdCAM Fc Chimera Protein, CF",R&D Systems,Cat# 993-MC-050 Fixation Buffer,BioLegend,Cat# 420801 Intracellular Staining Permeabilization Wash Buffer (10x),BioLegend,Cat# 421002 FLIPR Calcium 4 Assay

(45) Naturally self-reactive B cells are poised to cross the selection barrier into autoimmune germinal centersCell ReportsApril 8, 2026Danni Yi-Dan Yi-Dan Zhu, Maya Sangesland, Vintus Okonkwo, Zhenrui Zhang, Victoria Rosado, Larance Ronsard, Caitlin McCarthy, Caroline Alexander, Ralston M. Barnes, Daniel Rohrer, et al.dye Fixable live/dead stain Efluor 780,eBioscience,cat# 65–0865-18 Fixation buffer,Biolegend,cat# 420801 HBSS (Hanks Balanced Salt Solution),Corning,cat# 21–022-CV "HEPES, liquid 1M solution",Corning,cat#

(46) A 3D microfluidic model of exchange between perfused blood and lymphatic microvascular networksLab on a ChipApril 7, 2026Delaney Gray-Scherr, Terry Ching, Katherine Beran, Sarah C. Adams, Emily Davis, Abraham C. I. van Steen, Trisha Raman, Wilson W. Wong, Jeroen Eyckmans, Christopher S. Chenimaging studies. Immunostaining and imaging Devices were fixed with 4% paraformaldehyde (BioLegend, 420801) in PBS for 15 minutes, followed by PBS wash and permeabilization with 0.1% Triton X-100 (Sigma, T8787)

(47) EBV Triggers a Distinct Antiviral Response in HMC3 CellsbioRxivApril 7, 2026Noah E. Berkowitz, Alexander Nosov, Mark Nosov, Fryda Solis Roldan, Anuj Ahuja, Miranda McGaskey, Ethel Cesarman, Douglas F. Nixon, Nicholas Dopkinsroom temperature conditions using a fixation buffer containing 4% paraformaldehyde (BioLegend, Cat# 420801). Fixation buffer was then decanted and 200μL of DAPI Fluoromount-G® (SouthernBiotech, Cat# 0100–20)

(48) A periplasmic protein complex mediates arabinofuranosyltransferase activity and intrinsic drug resistance in Mycobacterium tuberculosisSci AdvApril 3, 2026Thaís Klevorn, Christopher Brown, Christine D. Hardy, Bonnie J. Cuthbert, Amanda Spencer, Adrián Jinich, Luming Chan, Shiva K. Angala, Jordan Manzer, Jessica Mendoza, et al.supernatant containing DMN-Tre was discarded. Samples were fixed with 4% paraformaldehyde (PFA; BioLegend, #420801) for 4 hours at room temperature. After two washes in PBS with 0.05% Tween 80, bacteria were resuspended

(49) Krüppel-like factors 2 and 3 regulate T cell exhaustion by directing T cell residency and migrationImmunityMarch 27, 2026Jian Shen, Ryan J. Brown, Yuqi Zhang, Kexin Gai, Ashley K. Brown, Ashley M. Bauer, Ashley Zhu, Siying Lin, Hongshen Niu, Arjun Kharel, et al.19853 P3 Primary Cell 96-well Nucleofector Kit,Lonza,Cat# V4SP-3096 Fixation Buffer,BioLegend,Cat# 420801 True-Nuclear Transcription Factor Buffer Set,BioLegend,Cat# 424401 Foxp3 / Transcription Factor Staining

(50) Recombinant human adenovirus type 5 synergizes with anti-PD-L1 antibody to promote anti-hepatocellular carcinoma effects through multilevel remodeling of the immune microenvironmentFrontiers in ImmunologyMarch 24, 2026Qiu Zhao, Min Xiao, Jian Ma, Xiaoqian Li, Cong Fu, Wenjing Ji, Lingna Ni, Jialin Fan, Qianqian Gao, Yanzhi BiAPC-CD86 (BioLegend, 105011), Alexa Fluor® 700-CD206 (BioLegend, 141733), fixation buffer (BioLegend, 420801), and intracellular staining permeabilization wash buffer (BioLegend, 421002). 2.7 HE, immunohistochemistry The

(51) Peripheral immune landscape in pancreatic ductal adenocarcinoma reveals expansion of effector states with disease progressioniScienceMarch 20, 2026Chen-Hsuan Yang, Yun-Yi Lung, Chieh-Chang Chen, I-Hsuan Lo, Chia-Jung Lien, Wei-Kai Wu, Ming-Shiang Wu, Wei-Chih Liao, Jian-Da Linstain,NanoEnTek,Cat# EBT-001 Brilliant Stain Buffer,BD Biosciences,Cat# 566385 Fixation Buffer,Biolegend,Cat# 420801 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, No critical

(52) Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicronJ ImmunolMarch 18, 2026Robert W P Glowacki, Jessica M Till, Orion D Brock, Vladimir Makarov, Morgan J Engelhart, Philip P Ahernby pipetting in 100 µl of a commercially available formaldehyde-based fixative (BioLegend, Cat. no. 420801; used when no intracellular staining was subsequently performed) or (ThermoFisher, Cat. # 00-5123-43

(53) Comprehensive single-cell metabolic profiling identifies targets to sensitize triple-negative breast cancer to chemo-immunotherapyCell Rep MedMarch 17, 2026Ying Xu, Xin-Yi Liu, Hang Zhang, Li Chen, Han Wang, Zhi-Ming Shao, Yi Xiao, Yi-Zhou Jiang420302 Intracellular Staining Permeabilization Wash Buffer (10x),BioLegend,Cat# 421002 Fixation Buffer,BioLegend,Cat# 420801 Cyto-Last™ Buffer,BioLegend,Cat# 422501 Cell Staining Buffer,BioLegend,Cat# 420201 Opal Polaris 7-Color

(54) Symbiotic exclusivity between CLOCK and TFPI2 drives stemness and immunosuppression in glioblastoma modelsJ Clin InvestMarch 17, 2026Fei Zhou, Lizhi Pang, Yang Liu, Fatima Khan, Peiwen Chen(BioLegend, #505850). After washing with FACS buffer, cells were incubated with fixation buffer (BioLegend, #420801). Then the labeled cells were analyzed using BD FACSymphony flow cytometer and FlowJo v10.8.1 software. Proliferation

(55) Selenium-loaded sustained-release schizophyllan alleviates pancreatic and pulmonary inflammatory damage in type 1 diabetes mellitus by modulating gut microbiota and T cell balanceJournal of NanobiotechnologyMarch 14, 2026Junming Luo, Siying Pei, Zhiying Ai, Runqing Rao, Zhuoyuan Yang, Xin Guo, Zeyi Ai, Lilin Fan, Fang ZouIntracellular staining was performed after permeabilization with fixation/permeabilization concentrate (420801, BioLegend, USA) mixed with diluent at a 1:3 ratio, followed by treatment with permeabilization buffer

(56) Prolonged Wnt3a exposure tolerizes macrophages to inflammatory stimuliFront ImmunolMarch 12, 2026Megan L. Tigue, Rincon Jagarlamudi, Channing Chi, Diana Diaz, Hua-Chang Chen, Quanhu Sheng, Sheau-Chiann Chen, Anna Schwarzkopf, Jeremy A. Goettel, Heather H. Pua, et al.temperature in the dark. After surface staining, cells were fixed with Fixation buffer (BioLegend, 420801) for 20 minutes in the dark and resuspended in 1X Intracellular Staining Permeabilization Wash Buffer

(57) Site-specific profiling of structure and function of Igµ B cell receptor glycansNature CommunicationsMarch 5, 2026M. D. Holborough-Kerkvliet, L. Hafkenscheid, S. Kroos, R. Biersteker, R. van de Wetering, O. Singh, E. Fadda, R. T. N. Tjokrodirijo, P. A. van Veelen, M. Wuhrer, et al.F(ab’)2 (Southern Biotech, #2062-01 and #2072-01, respectively) at 37 ⁰C. Fixation buffer (BioLegend, #420801) was used to fix the cells and was added immediately after the stimulation time passed. Cells were washed

(58) NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumorsNature CommunicationsFebruary 27, 2026Dominik Humer, Victoria Klepsch, Dietmar Rieder, Isabel Hölzl, Daniel Schreiber, Viktor Lang, Jiří Koutník, Sebastian Peer, Tajana Sajinovic, Viana Wille, et al.surface antibodies as stated above. Thereafter cells were fixed using the fixation buffer from Biolegend (420801) for 20 minutes at RT. After fixation, cells were permeabilized by washing twice with the kit’s Perm/Wash

(59) Investigating the Immune Effects of Radiotherapy in Non-Small Cell Lung Cancer—Results of the PD-RAD StudyOncology ResearchFebruary 24, 2026Shuhui Cheng, Tiana Kordbacheh, Antonia Banyard, Anshuman Chaturvedi, Diego Sanchez Martinez, Crispin T. Hiley, Maggie Harris, Clara Chan, Corinne Faivre-Finn, Timothy M. Illidge, et al.were washed in CSB and fixed in 1 mL of 4% paraformaldehyde (PFA) in PBS (fixation buffer, Biolegend, 420801) per 3 × 106 cells, for a minimum of 18 h. Finally, per 3 × 106 cells, 1 µL of iridium solution (10

(60) Long-acting interleukin-7 improves the efficacy of oncolytic viral therapy in glioblastomaNature CommunicationsFebruary 17, 2026Yuping Derek Li, David A. Giles, Yi Huang, Ashwani Kesarwani, Tong Hu, Logan Page, Amanda de Andrade Costa, Jingqin Luo, Alexandra Wolfarth, Donghoon Choi, et al.controls were used for setting population gates. For all staining procedures, cells were fixed (Biolegend, 420801), and permeabilization (Biolegend, 421002) was performed if intracellular staining was necessary. Absolute

Be the First to Write a Review!

Fixation Buffer from BioLegend

Description

BioLegend

Citations (580)

Reviews

Fixation Buffer from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map