Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(81) Conserved phenotype and function of human brain border-associated macrophages in iPSC-derived modelsbioRxiv : the preprint server for biologyDecember 13, 2025Barr HJ, Depp C, Hingerl M, Nguyen T, Reimertz JM, Connolly E, Patel J, Doman JL, Marsh SE, Stevens B.Briefly, mice were transcardially perfused with 10mL of HBSS and then 20mL of fixation buffer (BioLegend #420801). Brain and leptomeningeal samples were dissected as described above and then incubated in a fixation

(82) The circadian clock regulates scavenging of fluid-borne substrates by brain border-associated macrophagesbioRxiv : the preprint server for biologyDecember 11, 2025Barr HJ, Caldwell SK, Reimertz JM, Depp C, Walker AJ, Marsh SE, Gupte AS, Hingerl M, Patel J, Park SO, et al.mice were transcardially perfused using 10mL of HBSS followed by 20mL of fixation buffer (BioLegend #420801). All perfusion materials were kept ice cold throughout the procedure. Brains were dissected and incubated

(83) Double stranded RNA sensing is silenced during early embryonic developmentNat CommunDecember 11, 2025Jeroen Witteveldt, Zicong Liu, Ana Ariza-Cosano, Christian Ramirez, Jessica L. Walters, Pilar G. Marchante, Lars Maas, Elias T. Friman, Alasdair Ivens, Toma Tebaldi, et al.resuspended in FACS buffer (PBS with 1% FBS). Cells were pelleted and resuspended in Fixation buffer (420801, BioLegend) and incubated for 15’ at 4 °C, washed twice with Intracellular Staining Permeabilisation

(84) Activation of stimulator of interferon genes (STING) and inhibition of vascular endothelial growth factor receptor (VEGFR) by telatinib induce antitumor activityJournal of Biological ChemistryDecember 9, 2025Yi Wang, Yanfei Hou, Jing Han, Zhengyin Zhang, Yiyang Cheng, Jiaming Yang, Luqiu Mou, Shilong Fan, Peiyuan Liu, Kehong Chen, et al.Fixable Viability Dye eFluor 506 (Invitrogen, 65-0866-14), and then fixed in Fixation Buffer (Biolegend, 420801). After washing twice, cells were analyzed by BD LSRFortessa Analyzer (BD Biosciences) The results were

(85) The role of substance P in Th17/Treg imbalance and ocular surface damage in chronic allergic conjunctivitisJ Transl MedDecember 1, 2025Jiayu Bao, Ya Wen, Binge Wu, Yaqiong Li, Siyuan Li, Fengyang Lei, Lei Tian, Xiaoniao Chen, Ying Jie#421403), Intracellular Staining Permeabilization Wash Buffer (10X) (Cat. #421002), Fixation Buffer (Cat. #420801), Cell Activation Cocktail (with Brefeldin A) (Cat. #423303), and Cell Staining Buffer (Cat. #420201),

(86) Dynamic control of autoreactive B cells from spontaneous germinal centersThe Journal of ImmunologyDecember 1, 2025Kristine Oleinika, Alexia Correia Correia Ferreira, Selma Mouftakir, Carlos Castrillon, Lisa Madungwe, Usha Nair, Facundo D. Batista, Michael C. Carroll(4°C for 20min). After a final wash, cells were fixed in 100μL per well of fixation buffer (BioLegend, 420801, 4°C for 20min), washed again, and resuspended in 200μL per well of FACS buffer. Samples were acquired

(87) Single-cell multiomics reveals macrophage-derived IL-23 and CXCL9/10 drive pathogenic IFNG + IL17 + T cells in immunotherapy-related colitisJournal for ImmunoTherapy of CancerNovember 29, 2025Zhongshun Tang, Jiaolong Shi, Shuai Xu, Sheng Liu, Wenqian Yin, Yuyang Xiang, Junlin Li, Zhanke He, Xingxing Yao, Zhou Li, et al.Sigma-Aldrich) for 4 hours. After surface staining, cells were fixed using the fixation buffer (Biolegend Cat#420801) and permeabilized using Permeabilization Wash Buffer (Biolegend Cat#421002) according to the manufacturer’s

(88) Targeting VCP enhances colorectal cancer therapy through STING stabilizationJournal for ImmunoTherapy of CancerNovember 19, 2025Hongdao Zhu, Fujing Ge, Jingyu Dai, Shuyu Yang, Tao Yuan, Churun Zheng, Xin Dong, Ronggui Hu, Tianhua Zhou, Qiaojun He, et al.staining, cells previously stained with surface antibodies were permeabilized using fixation buffer (#420801; BioLegend) and permeabilization buffer (#421002; BioLegend) according to the manufacturer’s protocol,

(89) Metabolomic and transcriptomic profiling of HNSCC identifies AMIGO2 as a therapeutic target modulating tumor microenvironmentnpj Precision OncologyNovember 18, 2025Gan Liu, Xinfeng Yao, Yuchen Hou, Wentao Deng, Liyu Zhang, Shutong Li, Panpan Huang, Wenjiao Chang, Haiyan Huang, Lan Shi, et al.staining, the cells were fixed and permeabilized using cell permeabilization reagent (BioLegend, Cat. 420801) and fixation buffer (BioLegend, Cat. 421002). Subsequently, intracellular staining was performed with

(90) Long noncoding RNA LncBAR enhances BRG1 protein to promote cardiomyocyte cell cycle progression and cardiac repairnpj Regenerative MedicineNovember 17, 2025Jun Li, Huanhuan Cai, Yufan Chen, Ruiqi Pi, Lilin Xiang, Zhibing Lu, Yan Zhou, Li Wangby trypsin digestion at 37 °C for 3 min and fixed and permeabilized by fixation buffer (Biolegend, 420801), then incubated with antibodies listed in Supplementary Table 2 at RT for 30 min at concentrations

(91) Beclin1 deficiency unlocks cardiac lineage commitment through convergent Wnt and BMP signaling activationStem Cell Research & TherapyNovember 13, 2025Lilin Xiang, Zhenhua Jia, Zhangyi Yu, Lenan Wang, Huanhuan Cai, Tong Zhang, Zhibing Lu, Li Wangtrypsin digestion at 37 °C for 5 min. Cells were fixed and permeabilized with Fixation Buffer (Biolegend, 420801), then incubated with primary antibody against α-actinin and corresponding fluorescent secondary antibodies.

(92) Controlling intracellular processing to enhance spherical nucleic acid immune stimulationProceedings of the National Academy of SciencesNovember 11, 2025Janice Kang, Michelle H. Teplensky, Jasper W. Dittmar, Michael Evangelopoulos, Jeongmin Hwang, Chad A. MirkinPBS, centrifuged for 5 min at 1200 rpm, aspirated and fixed with 100 μL of fixation buffer (BioLegend, 420801) for 15 min at 4°C. The fixation buffer was washed by adding 600 μL of PBS, centrifuging at 1200 rpm

(93) A Semi-Automated Imaging Flow Cytometry Workflow for High-Throughput Quantification of Compound Internalization with IDEAS and FluoSta SoftwareMethods and ProtocolsNovember 9, 2025Kirill Elfimov, Ludmila Gotfrid, Alina Nokhova, Mariya Gashnikova, Dmitriy Baboshko, Aleksei Totmenin, Aleksandr Agaphonov, Natalya Gashnikova(Thermo Fisher Scientific, Waltham, MA, USA; Cat. No. MA1-065-A647).Fixation Buffer (BioLegend, San Diego, CA, USA; Cat. No. 420801).Intracellular Staining Permeabilization Wash Buffer (BioLegend, San Diego, CA, USA; Cat. No. 421002).Cell Staining Buffer

(94) Myeloid ADAM17 induces ST2+ T regulatory cells that limit obesity-induced metabolic inflammation through changes in both membrane and soluble TNFBiomedicine & PharmacotherapyNovember 1, 2025Joseph C. Lownik, Matthew P. Zellner, Jared Farrar, Anuj Tharakan, Francesco S. Celi, Daniel H. Conrad, Rebecca K. MartinTable 1) for 45 min on ice. Cells were washed with FACS buffer and fixed in Fixation Buffer (BioLegend, 420801) for 10 min, or secondaries were added and incubated for 30 min on ice followed by washing and fixation.

(95) Immunomodulation of behavior impairment via spleen-specific targeting lipid nanoparticles in a MeCP2 transgenic mouse modelScientific ReportsOctober 31, 2025Shu Zhang, Yaxi Li, Bowen Yang, Aygun Teymur, Fatin Atrooz, Sheereen Majd, Chandra Mohan, Samina Salim, Tianfu Wusurface staining antibodies. Afterward, the cells were fixed with fixation buffer (Biolegend, catalog#420801), permeabilized using Permeabilization Wash Buffer (Biolegend, catalog#421002), and incubated with MeCP2

(96) Natural killer cells and IFN-γ protect against liver injury during HAV infection in miceJournal of VirologyOctober 23, 2025Ichiro Misumi, You Li, Takayoshi Shirasaki, Lixin Yang, Maryna Kapustina, Stanley M. Lemon, Jason K. Whitmireagainst Fc-receptors. For intracellular staining, cells were fixed with fixation buffer (BioLegend; #420801) and permeabilized using Permeabilization Wash buffer (BioLegend; #421002) and incubated with antibodies

(97) ACOX2 destabilizes the MRE11-RAD50-NBS1 complex and boosts anticancer immunity via the cGAS-STING pathway in clear cell renal cell carcinomaMol CancerOctober 21, 2025Shiqi Ye, Wenhao Xu, Zheqi Chen, Chuanying Zhu, Qintao Ge, Jiahe Lu, Kun Chang, Xi Tian, Aihetaimujiang Anwaier, Shuxuan Zhu, et al.suspension with cell staining buffer (Biolegend, 420201), fixation with fixation Buffer (Biolegend, 420801), permeabilization with intracellular staining permeabilization wash buffer (Biolegend, 421002). Then,

(98) M1-like macrophages regulate T cell infiltration in colorectal cancer through P2X4 receptoriScienceOctober 17, 2025Kun Zhou, Xintian Zhang, Yu Liang, Han Yao, Yichao Hou, Xingming Zhang, Leilei Du, Wenfeng Wang, Jianhua Wang, Xiangjun Meng(America),Cat# M8823 FLAG peptide,Sigma-Aldrich (America),Cat# F4799 Fixation Buffer,BioLegend (America),Cat# 420801 Permeabilization Wash Buffer,BioLegend (America),Cat# 421002 FastPure EndoFree Plasmid Maxi Kit,Vazyme

(99) Modulating Subcellular Localization to Preserve the Stability and Functionality of Intracellular NanobodiesAntibodies (Basel)October 16, 2025Wenli Sun, Keke Huang, Yaping Cheng, Ailing Huang, Yu Kong, Jun Lu, Tianlei Ying, Yanling WuFlow Cytometry Analysis Following transfection, harvested cells were fixed in fixation buffer (cat#420801, Biolegend, San Diego, CA, USA) at 37 °C for 20 min in the dark, then washed twice with Intracellular

(100) Mirabegron treatment reduces myofibroblasts and CXCR2 expression in adipose tissue in obesityMol MedOctober 14, 2025Brian S. Finlin, Hasiyet Memetimin, Philip M. Westgate, Jin Chen, Esther E. Dupont-Versteegden, Philip A. KernTGFβ2 as described above. After treatment, the cells were incubated with fixation buffer (BioLegend, 420801) for 20 min in the dark at room temperature, washed with PBS, blocked with staining buffer (#554656,

Be the First to Write a Review!

Fixation Buffer from BioLegend

Description

BioLegend

Citations (580)

Reviews

Fixation Buffer from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map