Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC,ICC
Quantity100ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(81) Inhibiting Glycan Degradation Prevents HIV-Induced Inflammaging and Cognitive ImpairmentbioRxivJanuary 16, 2026Leila B. Giron, Alejandra Borjabad, Eran Hadas, Janeway Granche, Erika G. Marques de Menezes, Thomas A. Premeaux, Hongxia He, Stephen T. Yeung, Shalini Singh, Courtney Friday, et al.After incubation, cells were washed with PBS-2%FBS, fixed in PFA (Fixation buffer, Biolegend; Catalog# 420801), and analyzed by flow cytometry (BD Biosciences LSRII). Cytokines were measured in the supernatant

(82) TDP-43 dysfunction leads to the accumulation of cryptic transposable element-derived exons, crypTEs, in iPSC derived neurons and ALS/FTD patient tissuesbioRxivJanuary 13, 2026Isobel Bolger, Regina Shaw, Oliver H Tam, Cláudio Gouveia Roque, Christopher A Jackson, Kathryn O’Neill, NYGC ALS Consortium, Colin Smith, Hemali Phatnani, Karthick Natarajan, et al.μ-Slide 4-Well chamber slides (ibidi, Cat. no. 80426), fixed with Fixation Buffer (BioLegend, Cat. no. 420801) for 15 minutes at room temperature, washed with PBS (no calcium and magnesium), and blocked with Intercept®

(83) Serial Expression of Pro-Inflammatory Biomarkers in Acute Lung Injury During the Post-Resuscitation Periods in Rats with Cardiac ArrestInternational Journal of Molecular SciencesJanuary 13, 2026Han-Ping Wu, Kuan-Miao Lin, Mao-Jen Lin(202103, Biolegend). To detect the intracellular marker Foxp3, samples were treated with fixation buffer (420801, BioLegend) and permeabilization buffer (421002, BioLegend) and then stained with PE anti-mouse/rat/human FOXP3

(84) Butyrophilin 2A2 promotes T cell immunoregulation via CD45 phosphatase activation and protects against murine autoimmune glomerulonephritis and pregnancy lossNature communicationsJanuary 8, 2026Ali S, Berg AH, Yamashita M, Covarrubias AE, Mundell J, Shah PN, Zhang R, Dupont V, Shin BH, Yang S, et al.were stained with surface antibodies for 25 min at RT, washed and then fixed with Fixation Buffer (420801; Biolegend) for 20 min at RT and washed with 1X Intracellular Staining Permeabilization Wash Buffer

(85) Single‐Nucleus Multi‐Omics Reveals Hypoxia‐Driven Angiogenic Programs and Their Epigenetic Control in Sinonasal Squamous Cell CarcinomaAdvanced ScienceJanuary 7, 2026Chaelin You, Jaewoo Park, Jung Yeon Jang, Junho Noh, Jaehyun Lee, Geunho Kwon, Myeong Sang Yu, Yoo‐Sam Chung, Seung‐Jun Lee, Keunsoo Kang, et al.Catalog no. 12604013). Cells were fixed using pre‐warmed (37 °C) fixation buffer (BioLegend, Catalog no. 420801) at 37 °C for 15 min. Following washing, permeabilization was carried out using pre‐chilled (−20 °C)

(86) Suxiao Jiuxin Pill Promotes Post-Myocardial Infarction Cardiac Repair by Enhancing Regulatory T Cell Function via the ST2L Signaling PathwayJournal of Inflammation ResearchJanuary 6, 2026Lusha Zhang, Ying Guo, Yuming Fan, Ting Liu, Tianyu Wang, Mengyao Li, Shaoxia Wang, Qi Yu, Hong Wang(506915, Biolegend) was performed using the Cell Activation Cocktail (423304, BioLegend). Fixation (420801, BioLegend) and Intracellular Staining Permeabilization Wash Buffer (10X) (421002, BioLegend) were used

(87) Suxiao Jiuxin Pill Promotes Post-Myocardial Infarction Cardiac Repair by Enhancing Regulatory T Cell Function via the ST2L Signaling PathwayJ Inflamm ResJanuary 6, 2026Lusha Zhang, Ying Guo, Yuming Fan, Ting Liu, Tianyu Wang, Mengyao Li, Shaoxia Wang, Qi Yu, Hong Wang(506915, Biolegend) was performed using the Cell Activation Cocktail (423304, BioLegend). Fixation (420801, BioLegend) and Intracellular Staining Permeabilization Wash Buffer (10X) (421002, BioLegend) were used

(88) Adaptive-like CAR-iPSC-CD4⁺ T cells outperform CD8⁺ counterparts in sustained ALL controlInflammation and RegenerationJanuary 3, 2026Qingyi Guo, Chaoqi Zhang, Bo Wang, Shoichi Iriguchi, Akihiro Ishikawa, Atsutaka Minagawa, Tomoko Ishii, Yohei Kawai, Shin Kanekoon ice for 30 min. Intracellular staining was carried out using Fixation Buffer (BioLegend, Cat. No. 420801) and Intracellular Staining Perm Wash Buffer (BioLegend, Cat. No. 421002), following the manufacturer’s

(89) Microglial TBK1 Signaling Promotes Breast Cancer Brain MetastasisCancer ResearchJanuary 2, 2026Fatima Khan, Edgar Petrosyan, Yang Liu, Atousa Bahrami, Erfan Taefi, Changiz Geula, Maciej S. Lesniak, Peiwen Chenanalysis. For intracellular detection of P-TBK1, cells were fixed with fixation buffer (BioLegend, #420801), permeabilized with Perm buffer, and stained with P-TBK1 antibody (CST, #5483S, RRID:AB_10693472),

(90) Microglial TBK1 Signaling Promotes Breast Cancer Brain MetastasisCancer ResJanuary 2, 2026Fatima Khan, Edgar Petrosyan, Yang Liu, Atousa Bahrami, Erfan Taefi, Changiz Geula, Maciej S Lesniak, Peiwen Chenanalysis. For intracellular detection of P-TBK1, cells were fixed with fixation buffer (BioLegend, #420801), permeabilized with Perm buffer, and stained with P-TBK1 antibody (CST, #5483S, RRID:AB_10693472),

(91) Tubule-derived CCN1 drives renal repair via α(v)β(5)-STAT6-ARG1-dependent reprogramming of macrophagesCell death & diseaseDecember 21, 2025Zhang N, Li C, Xin Y, Song Z, Li T, Zhao R, Gu M, Xu L, Wang Y, Man X, et al.F4/80, CD86, CCR2, and Ly6C for 15 minutes in the dark. Cells were fixed with 250 μL fixation buffer (#420801, Biolegend, San Diego, CA, USA), permeabilized with intracellular permeabilization buffer (#421002,

(92) Conserved phenotype and function of human brain border-associated macrophages in iPSC-derived modelsbioRxiv : the preprint server for biologyDecember 13, 2025Barr HJ, Depp C, Hingerl M, Nguyen T, Reimertz JM, Connolly E, Patel J, Doman JL, Marsh SE, Stevens B.Briefly, mice were transcardially perfused with 10mL of HBSS and then 20mL of fixation buffer (BioLegend #420801). Brain and leptomeningeal samples were dissected as described above and then incubated in a fixation

(93) The circadian clock regulates scavenging of fluid-borne substrates by brain border-associated macrophagesbioRxiv : the preprint server for biologyDecember 11, 2025Barr HJ, Caldwell SK, Reimertz JM, Depp C, Walker AJ, Marsh SE, Gupte AS, Hingerl M, Patel J, Park SO, et al.mice were transcardially perfused using 10mL of HBSS followed by 20mL of fixation buffer (BioLegend #420801). All perfusion materials were kept ice cold throughout the procedure. Brains were dissected and incubated

(94) Double stranded RNA sensing is silenced during early embryonic developmentNat CommunDecember 11, 2025Jeroen Witteveldt, Zicong Liu, Ana Ariza-Cosano, Christian Ramirez, Jessica L. Walters, Pilar G. Marchante, Lars Maas, Elias T. Friman, Alasdair Ivens, Toma Tebaldi, et al.resuspended in FACS buffer (PBS with 1% FBS). Cells were pelleted and resuspended in Fixation buffer (420801, BioLegend) and incubated for 15’ at 4 °C, washed twice with Intracellular Staining Permeabilisation

(95) Activation of stimulator of interferon genes (STING) and inhibition of vascular endothelial growth factor receptor (VEGFR) by telatinib induce antitumor activityJournal of Biological ChemistryDecember 9, 2025Yi Wang, Yanfei Hou, Jing Han, Zhengyin Zhang, Yiyang Cheng, Jiaming Yang, Luqiu Mou, Shilong Fan, Peiyuan Liu, Kehong Chen, et al.Fixable Viability Dye eFluor 506 (Invitrogen, 65-0866-14), and then fixed in Fixation Buffer (Biolegend, 420801). After washing twice, cells were analyzed by BD LSRFortessa Analyzer (BD Biosciences) The results were

(96) The role of substance P in Th17/Treg imbalance and ocular surface damage in chronic allergic conjunctivitisJ Transl MedDecember 1, 2025Jiayu Bao, Ya Wen, Binge Wu, Yaqiong Li, Siyuan Li, Fengyang Lei, Lei Tian, Xiaoniao Chen, Ying Jie#421403), Intracellular Staining Permeabilization Wash Buffer (10X) (Cat. #421002), Fixation Buffer (Cat. #420801), Cell Activation Cocktail (with Brefeldin A) (Cat. #423303), and Cell Staining Buffer (Cat. #420201),

(97) Dynamic control of autoreactive B cells from spontaneous germinal centersThe Journal of ImmunologyDecember 1, 2025Kristine Oleinika, Alexia Correia Correia Ferreira, Selma Mouftakir, Carlos Castrillon, Lisa Madungwe, Usha Nair, Facundo D. Batista, Michael C. Carroll(4°C for 20min). After a final wash, cells were fixed in 100μL per well of fixation buffer (BioLegend, 420801, 4°C for 20min), washed again, and resuspended in 200μL per well of FACS buffer. Samples were acquired

(98) Single-cell multiomics reveals macrophage-derived IL-23 and CXCL9/10 drive pathogenic IFNG + IL17 + T cells in immunotherapy-related colitisJournal for ImmunoTherapy of CancerNovember 29, 2025Zhongshun Tang, Jiaolong Shi, Shuai Xu, Sheng Liu, Wenqian Yin, Yuyang Xiang, Junlin Li, Zhanke He, Xingxing Yao, Zhou Li, et al.Sigma-Aldrich) for 4 hours. After surface staining, cells were fixed using the fixation buffer (Biolegend Cat#420801) and permeabilized using Permeabilization Wash Buffer (Biolegend Cat#421002) according to the manufacturer’s

(99) Targeting VCP enhances colorectal cancer therapy through STING stabilizationJournal for ImmunoTherapy of CancerNovember 19, 2025Hongdao Zhu, Fujing Ge, Jingyu Dai, Shuyu Yang, Tao Yuan, Churun Zheng, Xin Dong, Ronggui Hu, Tianhua Zhou, Qiaojun He, et al.staining, cells previously stained with surface antibodies were permeabilized using fixation buffer (#420801; BioLegend) and permeabilization buffer (#421002; BioLegend) according to the manufacturer’s protocol,

(100) Metabolomic and transcriptomic profiling of HNSCC identifies AMIGO2 as a therapeutic target modulating tumor microenvironmentnpj Precision OncologyNovember 18, 2025Gan Liu, Xinfeng Yao, Yuchen Hou, Wentao Deng, Liyu Zhang, Shutong Li, Panpan Huang, Wenjiao Chang, Haiyan Huang, Lan Shi, et al.staining, the cells were fixed and permeabilized using cell permeabilization reagent (BioLegend, Cat. 420801) and fixation buffer (BioLegend, Cat. 421002). Subsequently, intracellular staining was performed with

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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