Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(21) NDRG3 is essential for sustaining antigen-driven T cell responses by protecting against restimulation-induced cell deathJ ImmunolMay 14, 2026Brian J Stack, Susan M KaechFoxp3/Transcription Factor Staining Buffer Set,00-5523-00,Thermo Fisher Scientific Fixation Buffer,420801,BioLegend Lonza P3 kit,V4XP-3032,Lonza S.p. Cas9 Nuclease V3,1081059,Integrated DNA Technologies "Goat

(22) Development of a nano-emulsion and evaluation of its intradermal adjuvant function of Swine Influenza H3N2 VaccineVirology JournalMay 8, 2026Zhuoyu Ruan, Bihua Deng, Wenzhu Yin, Fang Ma, Yu Lu, Haiyan Wangsurface staining, cells were fixed and permeabilized using a commercial fixation/permeabilization kit (420801, 421002, BioLegend), and then stained intracellularly with an antibody against mouse IFN-γ (BV421, 505830,

(23) Maternal opioid use and hepatitis C infection disrupt the placental immune landscape and structureJCI InsightMay 8, 2026Heather E. True, Brianna M. Doratt, Sheridan B. Wagner, Delphine C. Malherbe, Nathan R. Shelman, Mahdi Eskandarian Boroujeni, Cynthia Cockerham, John M. O’Brien, Ilhem Messaoudi30 minutes in the dark at 4°C. Samples were fixed and permeabilized using BioLegend FixPerm (catalog 420801) at 4°C for 20 minutes and stained intracellularly for 4 hours for TNF-α (APC, clone: MAb11, RRID: AB_315261),

(24) Endogenous CD28 drives the persistent activity of CAR T cells in myeloma and lymphoma modelsBlood Cancer DiscovMay 5, 2026Mackenzie M. Lieberman, Jason H. Tong, Nkechi U. Odukwe, Colin A. Chavel, Gina G. Bishara, Kimberly M. Crasti, Megan M. Herr, Payal Goala, Terence J. Purdon, Rebecca Burchett, et al.following fixation and permeabilization according to manufacturer’s instructions (BioLegend, catalog #420801 & #421002). Antibodies used in phenotypic analysis are included in the Key Resources Table and gating

(25) A mouse model to study persistent fatigueResearch SquareMay 4, 2026Adam J. Janowski, Kazuhiro Hayashi, Ashley N. Plumb, Joseph B. Lesnak, Angela F. Smith, Lynn Rasmussen, Elizabeth Gross, Andrew A. Post, Giovanni Berardi, Marguerita E. Klein, et al.flow cytometry staining buffer and fixed using a 4% paraformaldehyde solution (BioLegend Fixation Buffer, Cat. #420801; San Diego, CA, USA). Samples underwent a final wash and were resuspended in flow cytometry staining

(26) Single-cell analysis highlights the significance of malignant cell IFN/MHC-II for immunotherapy response in head and neck squamous cell carcinomaCell Reports MedicineApril 21, 2026Michael Mints, Reilly A. Sample, Anuraag S. Parikh, Jesse M. Zaretsky, Zongtai Qi, Travis Law, Fudong Wang, Thomas F. Barrett, Riley Mullins, Ashley Reeb, et al.of phospho-STAT1, live cells were fixed at 37°C for 15 min in pre-warmed Fixation Buffer (BioLegend, 420801). Fixed cells were washed 3 times in PBS +0.5% BSA and subsequently permeabilized in pre-chilled True-Phos Perm Buffer

(27) Novel NK cell-like phenotype expressing CCR5 and its ligands elicits tumor-specific acquired immunity to carcinomatous peritonitis via host NK-derived IFN-γJ Immunother CancerApril 20, 2026Situo Zheng, Yui Harada, Yosuke Morodomi, Noriko Yasuda, Kenta Ishimoto, Yoshikazu YonemitsuUSA). After 6 hours, following surface staining, the cells were fixed with fixation buffer (BioLegend, 420801, California, USA) for 20 min at room temperature, then washed two times using 1x Intracellular Staining

(28) Sunitinib induces immunogenic cell death through eIF2α phosphorylation to potentiate immunotherapy in HCCiScienceApril 17, 2026Yimei Gu, Hanbing Mai, Peijian Huang, Hui Yuan, Mingxuan Sun, Huixin Gao, Chenyu Shang, Yangfen Ou, Jizhou Tan, Xianzhang Huang, et al.incubation, cells were washed for intracellular staining. Cells were then fixed with Fixation Buffer (Cat#420801, BioLegend) and permeabilized with Permeabilization Buffer (Cat#00-8333-56, Invitrogen). Cells were

(29) Enhanced Th1 Cellular Immunity Induced by an RSV-F mRNA Vaccine Rationally Designed Using NLP AlgorithmsVaccines (Basel)April 16, 2026Zhi-Wu Xia, Qi Tang, Jun-Jie Pan, Jing Liu, Lan-Xin Jia, Guo-Mei Zhang, Man-Ni Xie, Jia-Hao Zheng, Chuan-Shuo Lv, Lei Zhang, et al.CD8 (Biolegend, Cat. #100706) for 15–30 min at RT. After fixation (Biolegend Fixation Buffer, Cat. #420801) and permeabilization (Biolegend Intracellular Staining Permeabilization Wash Buffer, Cat. #421002),

(30) Payload Diversification Overcomes Resistance and Guides Sequential Antibody–Drug Conjugate Therapy in Breast CancerClinical Cancer ResearchApril 15, 2026Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Takeo Fujii, Chalothorn Wannaphut, Jennifer A. Maynard, Kyoji Tsuchikama, George W. Sledge, et al.then replaced with antibody-free culture medium, cells were fixed with fixation buffer (BioLegend, 420801), mounted with DAPI-containing medium (ibidi, 50011), and 0 h images were acquired. A parallel plate

(31) Phenanthrene Exposure Induces Immune Dysfunction and Metabolic Disruption in MiceACS OmegaApril 15, 2026Qiaojuan Liu, Yan Zhang, Fenfang Deng, Wenxue Li, Guangyu Yang, Jun Yuan, Yuehong Wei, Juntao Li, Lei Tanand permeabilized using the Intracellular Fixation/Permeabilization Buffer Set (BioLegend, Cat. No. 420801/421002), followed by staining with fluorochrome-labeled antibodies against IFN-γ and TNF-α. All stained

(32) Payload Diversification Overcomes Resistance and Guides Sequential Antibody-Drug Conjugate Therapy in Breast CancerClin Cancer ResApril 15, 2026Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Takeo Fujii, Chalothorn Wannaphut, Jennifer A. Maynard, Kyoji Tsuchikama, George W. Sledge, et al.then replaced with antibody-free culture medium, cells were fixed with fixation buffer (BioLegend, 420801), mounted with DAPI-containing medium (ibidi, 50011), and 0 h images were acquired. A parallel plate

(33) A Microbial Lipid‐ATP Synthase Axis Fuels NK Cell Antitumor ActivityAdvanced ScienceApril 13, 2026Kaiyuan Yu, Xinyu Sun, Wanxia Ma, Jianming Yang, Xuan Sun, Lisong Zhang, Yumeng Liu, Tianshu Ren, Qi Wang, Jingyu Wang, et al.cells and NK cells for 30 min at 4°C. Cells were fixed for 30 min with fixation buffer (Biolegend, 420801), followed by permeabilization using an intracellular staining permeabilization wash buffer (Biolegend,

(34) Unveiling alternate pathways for SARS-CoV-2 infection via extracellular vesicle-mediated transfer of ACE2 and TMPRSS2Nature CommunicationsApril 10, 2026Martha Rea-Moreno, Lu Tian, Tara N. Tavakol, Min-Chi Yang, Nicole M. Pek, Shubham Gulati, Helena Bugacov, Cristina Cusmai, Gbalekan Dawodu, Remi V. Klotz, et al.EPD01-01) in PBS). For intracellular staining, the cells were fixed using the Fixation Buffer (BioLegend, 420801) and permeabilized with an Intracellular Staining Permeabilization Wash Buffer (BioLegend, 421002) according

(35) DDR1 Promotes Immune Evasion in Colorectal Cancer by Orchestrating IL33-Mediated M2-like Polarization of Tumor-Associated MacrophagesCancer Immunol ResApril 10, 2026Xiaofan Duan, Gaoshaer Yeerkenbieke, Yanjun Feng, Mingwang Zhou, Yumei Zhang, Jiuli Zhou, Jin Liminutes, ice). For intracellular staining, cells were washed with PBS, fixed using a fixation buffer (#420801, BioLegend) for 30 minutes, and stained with an anti–mouse CD206. For macrophage phenotyping, BMDMs

(36) A Rasa3-Gαi signaling axis orchestrates B lymphocyte trafficking into and through lymphoid organsCell ReportsApril 9, 2026Chung Park, Il-Young Hwang, Kathleen Harrison, Ning-Na Huang, Margery Smelkinson, Sundar Ganesan, Han G. Kim, Pamela L. Schwartzberg, John H. Kehrl796-IC-050 "Recombinant Mouse MAdCAM Fc Chimera Protein, CF",R&D Systems,Cat# 993-MC-050 Fixation Buffer,BioLegend,Cat# 420801 Intracellular Staining Permeabilization Wash Buffer (10x),BioLegend,Cat# 421002 FLIPR Calcium 4 Assay

(37) Naturally self-reactive B cells are poised to cross the selection barrier into autoimmune germinal centersCell ReportsApril 8, 2026Danni Yi-Dan Yi-Dan Zhu, Maya Sangesland, Vintus Okonkwo, Zhenrui Zhang, Victoria Rosado, Larance Ronsard, Caitlin McCarthy, Caroline Alexander, Ralston M. Barnes, Daniel Rohrer, et al.dye Fixable live/dead stain Efluor 780,eBioscience,cat# 65–0865-18 Fixation buffer,Biolegend,cat# 420801 HBSS (Hanks Balanced Salt Solution),Corning,cat# 21–022-CV "HEPES, liquid 1M solution",Corning,cat#

(38) EBV Triggers a Distinct Antiviral Response in HMC3 CellsbioRxivApril 7, 2026Noah E. Berkowitz, Alexander Nosov, Mark Nosov, Fryda Solis Roldan, Anuj Ahuja, Miranda McGaskey, Ethel Cesarman, Douglas F. Nixon, Nicholas Dopkinsroom temperature conditions using a fixation buffer containing 4% paraformaldehyde (BioLegend, Cat# 420801). Fixation buffer was then decanted and 200μL of DAPI Fluoromount-G® (SouthernBiotech, Cat# 0100–20)

(39) A 3D microfluidic model of exchange between perfused blood and lymphatic microvascular networksLab on a ChipApril 7, 2026Delaney Gray-Scherr, Terry Ching, Katherine Beran, Sarah C. Adams, Emily Davis, Abraham C. I. van Steen, Trisha Raman, Wilson W. Wong, Jeroen Eyckmans, Christopher S. Chenimaging studies. Immunostaining and imaging Devices were fixed with 4% paraformaldehyde (BioLegend, 420801) in PBS for 15 minutes, followed by PBS wash and permeabilization with 0.1% Triton X-100 (Sigma, T8787)

(40) A periplasmic protein complex mediates arabinofuranosyltransferase activity and intrinsic drug resistance in Mycobacterium tuberculosisSci AdvApril 3, 2026Thaís Klevorn, Christopher Brown, Christine D. Hardy, Bonnie J. Cuthbert, Amanda Spencer, Adrián Jinich, Luming Chan, Shiva K. Angala, Jordan Manzer, Jessica Mendoza, et al.supernatant containing DMN-Tre was discarded. Samples were fixed with 4% paraformaldehyde (PFA; BioLegend, #420801) for 4 hours at room temperature. After two washes in PBS with 0.05% Tween 80, bacteria were resuspended

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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