Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(21) NMI Regulates Adipose Adaptive Thermogenesis Through TLR4/IRF3 Signaling to Promote ObesityAdvanced ScienceJune 2, 2026Ting‐Ting Li, Xin‐Yuan Zhao, Min Zhang, Zhuang‐Feng Weng, Xiao‐Ran Guo, Ying‐Fang Liu, Qiao‐Ping Wang, Huan‐Huan LiangFor intracellular marker staining, cells were first fixed with fixation buffer (Biolegend, USA, Cat#420801) at 4°C for 30 min, followed by incubation with antibodies diluted in intracellular permeabilization

(22) A glycan-based adjuvant expands the breadth and duration of protection of mRNA-based vaccinesNature immunologyJune 1, 2026Jena KK, Qu P, Baracco L, Saghaei S, Keerti, Allahyari Z, Boehmer D, Mitchell M, Dillen CA, Li HY, et al.two washing steps in FACS buffer. For fixation, cells were incubated in fixation buffer (BioLegend, 420801) for 10 minutes at room temperature, followed by a washing step in FACS buffer. Samples were acquired

(23) Multimodal sequencing identifies synergistic mechanisms driving resistance to neoadjuvant nivolumab treatment in hepatocellular carcinomaMolecular CancerJune 1, 2026Fanhong Zeng, Jiaxin Guo, Zheng Zeng, Vanilla Xin Zhang, Yu-Man Tsui, Po-Man Li, Abdullah Husain, Qingyang Zhang, Joyce Man-Fong Lee, Tan-To Cheung, et al.co-cultured at a 10:1 effector-to-target ratio for 48 h. THP1 were collected and fixed with fixation buffer (420801, BioLegend), followed by incubation with the following antibodies: CD68 (333809, BioLegend), CD86 (305406,

(24) Non-decameric NLRP3 reveals a TGN/MTOC-distal pathway of inflammasome activationNature CommunicationsMay 30, 2026María Mateo-Tórtola, Inga V. Hochheiser, Gaopeng Li, Lukas Funk, Atousa Hashemi, Xiao Liu, Jane Torp, Lena Erlebach, András Szolek, Jelena Grga, et al.by the Zen blue software. In brief, cells plated in coverslips (12 mm) were fixed in 4 % PFA in PBS (420801, Biolegend) at 37 °C for 15 min, then washed in PBS 3 times with 5 min interval between washes. Afterwards,

(25) Impact of liquid dosing and associated cell ‘drowning’ in a 3D human bronchial epithelial model used for protease exposure studiesFrontiers in ToxicologyMay 27, 2026Georgina Hopkins, David Sheffield, Hugh Barlow, Anja Lalljie, Stella Cochrane, Lucy C Faircloughwith PBS; 200 µL apically and 750 µL basally. Cells were then fixed with 4% paraformaldehyde (Cat no. 420801, Biolegend), 200 µL applied apically and 750 µL basally, for 30 min at room temperature. The fixation

(26) Evaluation of Safety and Immunogenicity of High-Dose Quadrivalent Seasonal Influenza Split Vaccine: A Preclinical StudyVaccines (Basel)May 17, 2026Lanxin Jia, Ran Qiu, Jing Liu, Bo Liu, Xuanxuan Nian, Yang Le, Xixin Han, Qingmei Zhang, Xuedan Li, Zheng Gong, et al.min). Each sample tube received 500 μL of Fixation Buffer (BioLegend, San Diego, CA, USA; Cat. No. 420801) to fix the cells, which were incubated at room temperature in the dark for 20 min. After fixation,

(27) Activation-gated, T cell-restricted silencing of Dapk1 enhances Bacille Calmette-Guérin-elicited protective CD4+ memoryiScienceMay 15, 2026Zixuan Liu, Weihuang Liu, Chenyu Zhao, Xuanchang Bai, Fangting Zhou, Hang Sun, Jiahe Shi, Pinru Chen, Min Liu, Qin Pansubstrate,Biosharp,Cat# BL523B Zombie NIRTM Fixable Viability Kit,Biolegend,Cat# 423105 Fixation Buffer,Biolegend,Cat# 420801 Intracellular Staining Perm Wash Buffer(10×),Biolegend,Cat# 421002 Brefeldin A,Absin,Cat# abs810012 Cell lysis buffer

(28) Proteostasis sustains T cell differentiation potential and tumor-infiltrating lymphocyte functionCellMay 14, 2026Nicole E. Scharping, Xuezhen Ge, Maria Inês Matias, Fulin Jiang, Allison Cafferata, Maximilian Heeg, Alexander Monell, Giovanni Galletti, Kitty P. Cheung, Angelica Rock, et al.magnesium",Corning,Cat#20-021-CV "1,4-Dithioerythritol ≥99.0%",Sigma-Aldrich,Cat# D8255-25G Fixation Buffer,BioLegend,Cat#420801 eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set,Invitrogen,Cat#00-5523-00 BD Cytofix/Cytoperm™

(29) NDRG3 is essential for sustaining antigen-driven T cell responses by protecting against restimulation-induced cell deathJ ImmunolMay 14, 2026Brian J Stack, Susan M KaechFoxp3/Transcription Factor Staining Buffer Set,00-5523-00,Thermo Fisher Scientific Fixation Buffer,420801,BioLegend Lonza P3 kit,V4XP-3032,Lonza S.p. Cas9 Nuclease V3,1081059,Integrated DNA Technologies "Goat

(30) Development of a nano-emulsion and evaluation of its intradermal adjuvant function of Swine Influenza H3N2 VaccineVirology JournalMay 8, 2026Zhuoyu Ruan, Bihua Deng, Wenzhu Yin, Fang Ma, Yu Lu, Haiyan Wangsurface staining, cells were fixed and permeabilized using a commercial fixation/permeabilization kit (420801, 421002, BioLegend), and then stained intracellularly with an antibody against mouse IFN-γ (BV421, 505830,

(31) Maternal opioid use and hepatitis C infection disrupt the placental immune landscape and structureJCI InsightMay 8, 2026Heather E. True, Brianna M. Doratt, Sheridan B. Wagner, Delphine C. Malherbe, Nathan R. Shelman, Mahdi Eskandarian Boroujeni, Cynthia Cockerham, John M. O’Brien, Ilhem Messaoudi30 minutes in the dark at 4°C. Samples were fixed and permeabilized using BioLegend FixPerm (catalog 420801) at 4°C for 20 minutes and stained intracellularly for 4 hours for TNF-α (APC, clone: MAb11, RRID: AB_315261),

(32) Endogenous CD28 drives the persistent activity of CAR T cells in myeloma and lymphoma modelsBlood Cancer DiscovMay 5, 2026Mackenzie M. Lieberman, Jason H. Tong, Nkechi U. Odukwe, Colin A. Chavel, Gina G. Bishara, Kimberly M. Crasti, Megan M. Herr, Payal Goala, Terence J. Purdon, Rebecca Burchett, et al.following fixation and permeabilization according to manufacturer’s instructions (BioLegend, catalog #420801 & #421002). Antibodies used in phenotypic analysis are included in the Key Resources Table and gating

(33) A mouse model to study persistent fatigueResearch SquareMay 4, 2026Adam J. Janowski, Kazuhiro Hayashi, Ashley N. Plumb, Joseph B. Lesnak, Angela F. Smith, Lynn Rasmussen, Elizabeth Gross, Andrew A. Post, Giovanni Berardi, Marguerita E. Klein, et al.flow cytometry staining buffer and fixed using a 4% paraformaldehyde solution (BioLegend Fixation Buffer, Cat. #420801; San Diego, CA, USA). Samples underwent a final wash and were resuspended in flow cytometry staining

(34) Single-cell analysis highlights the significance of malignant cell IFN/MHC-II for immunotherapy response in head and neck squamous cell carcinomaCell Reports MedicineApril 21, 2026Michael Mints, Reilly A. Sample, Anuraag S. Parikh, Jesse M. Zaretsky, Zongtai Qi, Travis Law, Fudong Wang, Thomas F. Barrett, Riley Mullins, Ashley Reeb, et al.of phospho-STAT1, live cells were fixed at 37°C for 15 min in pre-warmed Fixation Buffer (BioLegend, 420801). Fixed cells were washed 3 times in PBS +0.5% BSA and subsequently permeabilized in pre-chilled True-Phos Perm Buffer

(35) Novel NK cell-like phenotype expressing CCR5 and its ligands elicits tumor-specific acquired immunity to carcinomatous peritonitis via host NK-derived IFN-γJ Immunother CancerApril 20, 2026Situo Zheng, Yui Harada, Yosuke Morodomi, Noriko Yasuda, Kenta Ishimoto, Yoshikazu YonemitsuUSA). After 6 hours, following surface staining, the cells were fixed with fixation buffer (BioLegend, 420801, California, USA) for 20 min at room temperature, then washed two times using 1x Intracellular Staining

(36) Sunitinib induces immunogenic cell death through eIF2α phosphorylation to potentiate immunotherapy in HCCiScienceApril 17, 2026Yimei Gu, Hanbing Mai, Peijian Huang, Hui Yuan, Mingxuan Sun, Huixin Gao, Chenyu Shang, Yangfen Ou, Jizhou Tan, Xianzhang Huang, et al.incubation, cells were washed for intracellular staining. Cells were then fixed with Fixation Buffer (Cat#420801, BioLegend) and permeabilized with Permeabilization Buffer (Cat#00-8333-56, Invitrogen). Cells were

(37) Enhanced Th1 Cellular Immunity Induced by an RSV-F mRNA Vaccine Rationally Designed Using NLP AlgorithmsVaccines (Basel)April 16, 2026Zhi-Wu Xia, Qi Tang, Jun-Jie Pan, Jing Liu, Lan-Xin Jia, Guo-Mei Zhang, Man-Ni Xie, Jia-Hao Zheng, Chuan-Shuo Lv, Lei Zhang, et al.CD8 (Biolegend, Cat. #100706) for 15–30 min at RT. After fixation (Biolegend Fixation Buffer, Cat. #420801) and permeabilization (Biolegend Intracellular Staining Permeabilization Wash Buffer, Cat. #421002),

(38) Payload Diversification Overcomes Resistance and Guides Sequential Antibody-Drug Conjugate Therapy in Breast CancerClin Cancer ResApril 15, 2026Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Takeo Fujii, Chalothorn Wannaphut, Jennifer A. Maynard, Kyoji Tsuchikama, George W. Sledge, et al.then replaced with antibody-free culture medium, cells were fixed with fixation buffer (BioLegend, 420801), mounted with DAPI-containing medium (ibidi, 50011), and 0 h images were acquired. A parallel plate

(39) Phenanthrene Exposure Induces Immune Dysfunction and Metabolic Disruption in MiceACS OmegaApril 15, 2026Qiaojuan Liu, Yan Zhang, Fenfang Deng, Wenxue Li, Guangyu Yang, Jun Yuan, Yuehong Wei, Juntao Li, Lei Tanand permeabilized using the Intracellular Fixation/Permeabilization Buffer Set (BioLegend, Cat. No. 420801/421002), followed by staining with fluorochrome-labeled antibodies against IFN-γ and TNF-α. All stained

(40) Payload Diversification Overcomes Resistance and Guides Sequential Antibody–Drug Conjugate Therapy in Breast CancerClinical Cancer ResearchApril 15, 2026Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Takeo Fujii, Chalothorn Wannaphut, Jennifer A. Maynard, Kyoji Tsuchikama, George W. Sledge, et al.then replaced with antibody-free culture medium, cells were fixed with fixation buffer (BioLegend, 420801), mounted with DAPI-containing medium (ibidi, 50011), and 0 h images were acquired. A parallel plate

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Fixation Buffer from BioLegend

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Fixation Buffer from BioLegend

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