Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(161) Exogenous BMP9 promotes lung fibroblast HFL-1 cell activation via ALK1/Smad1/5 signaling in vitroExperimental and Therapeutic MedicineMay 4, 2021Yaqun Wang, Xiaonan Sima, Ying Ying, Yonghong Huangprotocol. Total RNA was reverse transcribed to cDNA using the Reverse Transcription System (cat. no. A3500; Promega Corporation). The temperature protocol for reverse transcription was 42˚C for 15 min, 95˚C

(162) Differential Signaling and Virus Production in Calu-3 Cells and Vero Cells upon SARS-CoV-2 InfectionBiomolecules & TherapeuticsMay 1, 2021Byoung Kwon Park, Dongbum Kim, Sangkyu Park, Sony Maharjan, Jinsoo Kim, Jun-Kyu Choi, Madhav Akauliya, Younghee Lee, Hyung-Joo Kwoninstructions. cDNA (50 µL) was synthesized using the Reverse Transcription System Kit (Catalog No. A3500, Promega, Madison, WI, USA). To quantify expression of the RNA-dependent RNA polymerase (RdRP) gene

(163) Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasmsCell reportsApril 13, 2021Daniels NJ, Hershberger CE, Gu X, Schueger C, DiPasquale WM, Brick J, Saunthararajah Y, Maciejewski JP, Padgett RA.20040525 AffinityScript,Agilent,Cat#600107 Q5 PCR mix,NEB,Cat#M0492 Reverse Transcription System,Promega,Cat#A3500 Deposited data,Deposited data,Deposited data LUC7-like seCLIP-seq,This Paper,ArrayExpress: E-MTAB-9709 LUC7-like

(164) Morusin inhibits the growth of human colorectal cancer HCT116‑derived sphere‑forming cells via the inactivation of Akt pathwayInternational journal of molecular medicineApril 1, 2021Zhou Y, Li X, Ye M.was used as a template to prepare cDNA (Reverse Transcription System, Promega Corporation; cat. no. A3500) according to the manufacturers' instructions. The amplification conditions were as follows: Stage 1

(165) Tanshinone IIA combined with CsA inhibit myocardial cell apoptosis induced by renal ischemia-reperfusion injury in obese ratsBMC complementary medicine and therapiesMarch 22, 2021Tai H, Jiang XL, Lan ZM, Li Y, Kong L, Yao SC, Song N, Lv MJ, Wu J, Yang P, et al.transcription was performed with total RNA (1 μg) and the M-MLV Reverse Transcriptase Kit (Promega A3500; Promega, Madison, WI, USA). Briefly, the total reaction volume (40 μL) was used in a Veriti 96 Well

(166) Intraperitoneal Administration of Short-Chain Fatty Acids Improves Lipid Metabolism of Long–Evans Rats in a Sex-Specific MannerNutrientsMarch 10, 2021Shrushti Shah, Tiffany Fillier, Thu Huong Pham, Raymond Thomas, Sukhinder Kaur Cheema#M610A 12358503; Promega, Madison, WI, USA). Reverse transcription was performed to generate cDNA (Cat #A3500; Promega, Madison, WI, USA), which was later amplified for quantitative real-time PCR (qPCR) using iQ-SYBR Green Supermix

(167) THC Reduces Ki67-Immunoreactive Cells Derived from Human Primary Glioblastoma in a GPR55-Dependent MannerCancersMarch 3, 2021Kolbe MR, Hohmann T, Hohmann U, Ghadban C, Mackie K, Zöller C, Prell J, Illert J, Strauss C, Dehghani F.Winooski, VT, USA). The generated cDNA (Reverse Transcription System, Promega GmbH, Walldorf, Germany, A3500) was amplified in a total volume of 20 µL containing 10 µL PCR-MasterMix (Promega Inc., M7505), 0.5

(168) TREM2 sustains macrophage-hepatocyte metabolic coordination in nonalcoholic fatty liver disease and sepsisThe Journal of clinical investigationFebruary 15, 2021Hou J, Zhang J, Cui P, Zhou Y, Liu C, Wu X, Ji Y, Wang S, Cheng B, Ye H, et al.obtained total RNA was then reverse-transcribed into cDNA using the Reverse Transcription System (Promega, A3500) or the Mir-X miRNA firststrand synthesis kit (Takara, 638313) according to the manufacturer’s instructions.

(169) Antiviral Mechanism of Tea Polyphenols against Porcine Reproductive and Respiratory Syndrome VirusPathogensFebruary 13, 2021Xun Wang, Wenjuan Dong, Xiaoxiao Zhang, Zhenbang Zhu, Yaosheng Chen, Xiaohong Liu, Chunhe Guofrom cultured cells using TRIzol reagent (Magen, Guangzhou, China). The Reverse Transcription System (A3500, Promega, Madison, WI, USA) was used for reverse transcription in 20 µL of reaction volume following

(170) Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF-κB acetylationExperimental and therapeutic medicineFebruary 1, 2021Li J, Sun L, Li Y.(Thermo Fisher Scientific, Inc.) and cDNA was synthesized using the Reverse Transcription System (cat. no. A3500; Promega Corporation) according to the manufacturer's protocols. qPCR was performed in an Applied Biosystems 7500 Real-Time PCR System

(171) Increased ROS-Dependent Fission of Mitochondria Causes Abnormal Morphology of the Cell Powerhouses in a Murine Model of Amyotrophic Lateral SclerosisOxidative medicine and cellular longevityJanuary 1, 2021Stein J, Walkenfort B, Cihankaya H, Hasenberg M, Bader V, Winklhofer KF, Röderer P, Matschke J, Theiss C, Matschke V.to the manufacturer's protocol. cDNA synthesis was performed with a reverse transcription system (#A3500, Promega, USA). Following the manufacturer's protocol, 1 μg tRNA and oligo(dT)15 primer were used. The

(172) MERS-CoV and SARS-CoV-2 replication can be inhibited by targeting the interaction between the viral spike protein and the nucleocapsid proteinTheranosticsJanuary 1, 2021Park BK, Kim J, Park S, Kim D, Kim M, Baek K, Bae JY, Park MS, Kim WK, Lee Y, et al.manufacturer's instructions. cDNA was prepared using a Reverse Transcription System kit (Catalog No. A3500, Promega, Madison, WI, USA). The primer sequences to quantify the RNA-dependent RNA polymerase (RdRP)

(173) Chronic Voluntary Alcohol Drinking Causes Anxiety-like Behavior, Thiamine Deficiency, and Brain Damage of Female Crossed High Alcohol Preferring MiceFrontiers in pharmacologyJanuary 1, 2021Xu H, Li H, Liu D, Wen W, Xu M, Frank JA, Chen J, Zhu H, Grahame NJ, Luo J.previously (Kim et al., 2014). 1 μg of total RNA was used for ﬁrst strand cDNA synthesis (Promega, A3500). Quantitative real-time RT-PCR was performed on a Light cycler 480 system (Roche) using a Power SYBR

(174) Treatment With the CSF1R Antagonist GW2580, Sensitizes Microglia to Reactive Oxygen SpeciesFrontiers in immunologyJanuary 1, 2021Soto-Diaz K, Vailati-Riboni M, Louie AY, McKim DB, Gaskins HR, Johnson RW, Steelman AJ.No., K0731). cDNA was created using the reverse transcription system (Promega Corporation, Cat No., A3500), and amplified using Sybr Green qPCR master mix (Applied Biosystems, Cat No., A25742), and primers

(175) Tanshinone IIA Combined With Cyclosporine A Alleviates Lung Apoptosis Induced by Renal Ischemia-Reperfusion in Obese RatsFrontiers in medicineJanuary 1, 2021Tai H, Jiang XL, Song N, Xiao HH, Li Y, Cheng MJ, Yin XM, Chen YR, Yang GL, Jiang XY, et al.Reverse transcription was performed with 1 μg total RNA and an M-MLV Reverse Transcriptase Kit (Promega A3500; Promega, Madison, WI, USA). Briefly, the 40-μl total reaction volume was used in a Veriti 96 Well Thermal

(176) Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial ImmunityFrontiers in immunologyJanuary 1, 2021Chowdhury D, Gardner JC, Satpati A, Nookala S, Mukundan S, Porollo A, Landero Figueroa JA, Subramanian Vignesh K.74104 QUICK-RNA™ MINIPREP KIT,Denville Scientific,Cat# R1055 Reverse Transcription System,Promega,REF#A3500 Probe Lo-Rox 2X qPCR Mix,RADIANT™,Cat#QP9020 CytoTox 96® NonRadioactive Cytotoxicity Assay kit Promega,Promega,REF#G1781 SuperSignal™ West Femto Maximum Sensitivity Substrate

(177) Pleural Mesothelial Cells Modulate the Inflammatory/Profibrotic Response During SARS-CoV-2 InfectionFrontiers in molecular biosciencesJanuary 1, 2021Matusali G, Trionfetti F, Bordoni V, Nardacci R, Falasca L, Colombo D, Terri M, Montaldo C, Castilletti C, Mariotti D, et al.to the manufacturer’s instructions. cDNA synthesis was generated using a reverse transcription kit (A3500) from Promega (Madison, WI), according to the manufacturer’s recommendations. cDNAs were amplified by

(178) NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis via inhibiting the CTHRC1/HIF-1α axisInternational journal of biological sciencesJanuary 1, 2021Chen L, Yang Y, Yan H, Peng X, Zou J.Invitrogen) from tissues and cells. Subsequently, RNA was reverse-transcribed into cDNA utilizing the A3500 Reverse Transcription System (Promega, Madison, WI, USA), and the cDNA was amplified utilizing the Fast

(179) Total Flavonoids of Rhizoma Drynariae Restore the MMP/TIMP Balance in Models of Osteoarthritis by Inhibiting the Activation of the NF-κB and PI3K/AKT PathwaysEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2021Chen GY, Chen JQ, Liu XY, Xu Y, Luo J, Wang YF, Zhou TL, Yan ZR, Zhou L, Tao QW.NanoDrop spectrophotometer (Thermo Scientific, USA). cDNA was obtained by a reverse transcription system (A3500, Promega, USA) according to the manufacturer's instructions and was then stored at −20°C until use. qPCR

(180) Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grapeBMC Plant BiologyDecember 1, 2020Huiling Wang, Ailing Yan, Lei Sun, Guojun Zhang, Xiaoyue Wang, Jiancheng Ren, Haiying Xuwere synthesized according to the manufacturer’s instruction of AMV reverse transcriptase (Promega A3500). Then, two-step qPCR was carried out in the CFX 96 RT-PCR system (Bio-Rad, Richmond, CA) using a SYBR

Be the First to Write a Review!