Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(201) Lipopolysaccharide Downregulates CD163 Expression to Inhibit PRRSV Infection via TLR4-NF-κB PathwayFrontiers in MicrobiologyMarch 25, 2020Zhenbang Zhu, Hui Zhang, Xiaoxiao Zhang, Sheng He, Wenjuan Dong, Xiaoying Wang, Yaosheng Chen, Xiaohong Liu, Chunhe Guousing the TRIzol reagent. RNA were reverse-transcribed into cDNA using Reverse Transcription System (A3500, Promega, United States) according to manufacturer’s instructions. Reverse-transcription products were

(202) ADAMTS1 and HSPG2 mRNA levels in cumulus cells are related to human oocyte quality and controlled ovarian hyperstimulation outcomesJournal of Assisted Reproduction and GeneticsMarch 1, 2020Yerong Ma, Jiamin Jin, Xiaomei Tong, Weijie Yang, Peipei Ren, Yongdong Dai, Yibin Pan, YinLi Zhang, Songying Zhangof each sample by reverse transcription using oligo (dT)15 and reverse transcriptase (Promega, USA, A3500), according to the manufacturer’s instructions, at 42 °C for 15 min, followed by 95 °C for 5 min in

(203) SLC27A5 deficiency activates NRF2/TXNRD1 pathway by increased lipid peroxidation in HCCCell death and differentiationMarch 1, 2020Gao Q, Zhang G, Zheng Y, Yang Y, Chen C, Xia J, Liang L, Lei C, Hu Y, Cai X, et al.RNA samples were reverse-transcribed into cDNA using the Moloney murine leukemia virus reverse transcriptase (A3500, Promega, Madison, USA). Quantitative Real-Time PCR analysis of target genes was performed using the

(204) Single‑cell RNA sequencing of t(8;21) acute myeloid leukemia for risk predictionOncology ReportsFebruary 18, 2020Qian Xiong et al.S1). RNA was extracted from samples and cDNA was reverse transcribed (Reverse Transcription System A3500; Promega Corporation) from RNA with TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). The

(205) Combined deficiency of SLAMF8 and SLAMF9 prevents endotoxin-induced liver inflammation by downregulating TLR4 expression on macrophagesCellular & Molecular ImmunologyFebruary 1, 2020Xiaokang Zeng, Guangao Liu, Wanwen Peng, Junming He, Chenxu Cai, Wei Xiong, Shasha Chen, Meixiang Yang, Zhongjun Dongusing a Trizol Kit (Invitrogen) and reverse transcribed using a reverse transcription system (Promega, A3500) according to the manufacturer’s instructions. Quantitative PCR was performed with SYBR green PCR Master

(206) Impaired lipid biosynthesis hinders anti-tumor efficacy of intratumoral iNKT cellsNature communicationsJanuary 23, 2020Fu S, He K, Tian C, Sun H, Zhu C, Bai S, Liu J, Wu Q, Xie D, Yue T, et al.Miniprep System (Promega Z6010) and was reverse transcribed with Reverse Transcription System (Promega A3500). Real-time qPCR was performed with PikoReal 96 (Thermo Scientific). In each experiment, samples were

(207) Scinderin suppresses cell proliferation and predicts the poor prognosis of hepatocellular carcinomaOncology LettersJanuary 8, 2020Bin Zhou, Tian‑Wei Chen, Ya‑Bo Jiang, Xu‑Biao Wei, Chong‑De Lu, Jing‑Jing Li, Dong Xie, Shu‑Qun ChengScientific, Inc.), according to the manufacturer's protocol. Total RNA was reverse transcribed to cDNA (cat. no. A3500; Promega Corporation). qPCR was subsequently performed using the CFX96 Real‑Time System (Bio‑Rad Laboratories,

(208) Conjugation of TLR7 Agonist Combined with Demethylation Treatment Improves Whole-Cell Tumor Vaccine Potency in Acute Myeloid LeukemiaInternational journal of medical sciencesJanuary 1, 2020Zhong G, Jin G, Zeng W, Yu C, Li Y, Zhou J, Zhang L, Yu L.the 'manufacturer's instruction. RT was performed using the Reverse Transcription System (Promega, #A3500, USA) on 1 mg of total RNA, and PCR amplifications were then performed using primers shown in Table

(209) PPARγ as an E3 Ubiquitin-Ligase Impedes Phosphate-Stat6 Stability and Promotes Prostaglandins E₂-Mediated Inhibition of IgE Production in AsthmaFrontiers in immunologyJanuary 1, 2020Wu J, Wang Y, Zhou Y, Wang Y, Sun X, Zhao Y, Guan Y, Zhang Y, Wang W.Invitrogen, Life Technologies). cDNA was synthesized using the AMV cDNA Reverse Transcription Kit (A3500; Promega, Madison, WI). For quantitative PCR, SYBR Green Supermix (11143ES50; YEASEN, Beijing, China)

(210) The Effect of Hedysarum multijugum Maxim.-Chuanxiong rhizoma Compound on Ischemic Stroke: A Research Based on Network and Experimental PharmacologyOxidative medicine and cellular longevityJanuary 1, 2020Yang K, Zeng L, Ge A, Shi Y, Zhu X, Liu W, Ge J.antibody, and goat anti-rabbit secondary antibody (Vazyme Biotech Co., Ltd); reverse transcription kit A3500 and real-time PCR mix A6001 (Promega Inc.); DNA marker (Fermentas Inc.); anhydrous ethanol, isopropanol,

(211) Immunomodulatory features of radiotherapy in lung carcinomaTranslational Cancer ResearchJanuary 1, 2020Ge Shen, Wei Wang, Yong Da, Dan Li, Li Bai, Jun Tai, Guangxian Liuon the included protocol. Reverse transcription was carried out with reagents from Promega (cat. no. A3500; USA) as directed by the manufacturer. Amplification was carried out on an AB 7500 Real-Time PCR System

(212) Menadione reduces CDC25B expression and promotes tumor shrinkage in gastric cancerTherapeutic advances in gastroenterologyJanuary 1, 2020Bona AB, Calcagno DQ, Ribeiro HF, Muniz JAPC, Pinto GR, Rocha CAM, Lacreta Junior ACC, de Assumpção PP, Herranz JAR, Burbano RR.DNA was manufactured using the Reverse Transcription System according to the manufacturer’s protocol (A3500; Promega, Madison, WI, USA), then amplified by RT-qPCR using TaqMan probes purchased as Assays-on-Demand

(213) Antitumor Efficacy of Oncolytic Herpes Virus Type 1 Armed with GM-CSF in Murine Uveal Melanoma XenograftsCancer management and researchJanuary 1, 2020Liu S, Liu F, Zhao M, Zhang J.Spectrophotometer. A total of 1 µg of RNA was reverse-transcribed using a Reverse Transcription System Kit (Promega A3500, Fitchburg, WI, USA) following the manufacturer’s instructions. The following primers were generated:

(214) Investigation of bovine coronavirus and bovine rotavirus by rapid diagnosis kit and RT-PCR in diarrheic calf fecesJournal of Istanbul Veterinary SciencesDecember 31, 2019Gülşah UYUNMAZ SAKLI, Oya BULUT, Mustafa HASÖKSÜZ, Hasan Hüseyin HADİMLİacid was isolated, and stored at -80oC. cDNA Synthesis: We used Reverse Transcription System (Promega A3500) to obtain cDNAs. In order to synthesize cDNA from the isolated viral RNA, we used the Promega Reverse Transcription System

(215) Targeting Histone Methyltransferase DOT1L by a Novel Psammaplin A Analog Inhibits Growth and Metastasis of Triple-Negative Breast CancerMolecular therapy oncolyticsDecember 20, 2019Byun WS, Kim WK, Han HJ, Chung HJ, Jang K, Kim HS, Kim S, Kim D, Bae ES, Park S, et al.USA), and 1 μg total RNA was reverse-transcribed using the Reverse Transcription System (catalog no. A3500; Promega, Madison, WI, USA) according to the manufacturer’s instructions. Real-time PCR was conducted

(216) Roles of β-catenin, TCF-4, and survivin in nasopharyngeal carcinoma: correlation with clinicopathological features and prognostic significanceCancer Cell InternationalDecember 1, 2019Pei-Ying Jin, Zi-Hui Zheng, Hong-Jie Lu, Jing Yan, Gui-Hong Zheng, Yuan-Lin Zheng, Dong-Mei Wu, Jun Luof total RNA was performed in accordance with instructions provided by Reverse Transcription System A3500 (Promega Corporation, Madison, WI, USA). A two-step PCR was conducted as follows: 40 cycles from initial

(217) Hypoxia destroys the microstructure of microtubules and causes dysfunction of endothelial cells via the PI3K/Stathmin1 pathwayCell & BioscienceDecember 1, 2019Huaming Cao, Dongsheng Yu, Xueyun Yan, Bing Wang, Zhiming Yu, Yu Song, Liang ShengPlus (Takara Bio Inc., Dalian, China) and was reverse-transcribed using M-MMLV Reverse Transcriptase (#A3500, Promega Biotech Co., Ltd, Beijing, China) according to the manufacturer’s protocols. RT-qPCR was performed

(218) Static magnetic field regulates Arabidopsis root growth via auxin signalingScientific ReportsDecember 1, 2019Yue Jin et al.cDNA was synthesized using oligo (dT) 18 from 1 μg RNA with a Reverse Transcription System (Promega, A3500). Quantitative real-time PCR was performed with gene-specific primers using a total volume of 20 μl

(219) Synergized regulation of NK cell education by NKG2A and specific Ly49 family membersNature communicationsNovember 1, 2019Zhang X, Feng J, Chen S, Yang H, Dong Z.Trizol reagent (Invitrogen) and then reverse transcribed into cDNAs using oligo d(T)n primers (Promega, A3500). Real-time PCR was performed with a Bio-Rad CFX96 Real-Time System using primers specific for Klra-family

(220) Molecular and biological characterization of some circulating strains of Newcastle disease virus in broiler chickens from Eastern Saudi Arabia in 2012-2014October-2019October 30, 2019Abdullah I. A. AlmubarakExtracted RNA was stored at −80°C until use to produce cDNA by the Reverse Transcription System (Cat # A3500, Promega, USA) according to the manufacturer’s instructions. Detection and genotyping of NDV Detection

Be the First to Write a Review!