Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(141) The novel TRPA1 antagonist BI01305834 inhibits ovalbumin-induced bronchoconstriction in guinea pigsRespiratory ResearchDecember 1, 2021Mariska P. M. van den Berg, Susan Nijboer-Brinksma, I. Sophie T. Bos, Maarten van den Berge, David Lamb, Martijn van Faassen, Ido P. Kema, Reinoud Gosens, Loes E. M. KistemakerNanoDrop ND-1000 spectrophotometer. Equal amounts of total mRNA were then reverse transcribed (Promega, #A3500), and cDNA was subjected to real-time qPCR (Westburg, Leusden, The Netherlands). Real time qPCR was

(142) Repeated exposure with short-term behavioral stress resolves pre-existing stress-induced depressive-like behavior in miceNature communicationsNovember 18, 2021Lee EH, Park JY, Kwon HJ, Han PL.transcription of total RNA (2 μg) was conducted in a volume of 20 μl using a reverse transcription system (A3500, Promega, MO, USA). Real-time PCR was performed with 10 μl of 2X iQ™ SYBR Green Supermix (#170882, Bio-Rad Laboratories

(143) Effects of whole-body vibration training in a cachectic C26 mouse modelScientific reportsNovember 3, 2021van der Ende M, Plas RLC, van Dijk M, Dwarkasing JT, van Gemerden F, Sarokhani A, Swarts HJM, van Schothorst EM, Grefte S, Witkamp RF, et al.Nanodrop technologies Wilmington, DE, USA). cDNA was synthesized using the Promega cDNA synthesis Kit (A3500, Promega Benelux B.V., Leiden, the Netherlands) following the manufacture’s instruction in a Hybaid

(144) Apoptosis Enhances the Replication of Human Coronavirus OC43VirusesNovember 1, 2021Maharjan S, Kang M, Kim J, Kim D, Park S, Kim M, Baek K, Lee Y, Kwon HJ.previously [25]. cDNA (50 μL) was synthesized with a Reverse Transcription System kit (Catalog No. A3500, Promega, Madison, WI, USA). To quantify the nucleocapsid (N) gene of HCoV-OC43, the following primers

(145) EGCG Inhibits Tumor Growth in Melanoma by Targeting JAK-STAT Signaling and Its Downstream PD-L1/PD-L2-PD1 Axis in Tumors and Enhancing Cytotoxic T-Cell ResponsesPharmaceuticals (Basel, Switzerland)October 26, 2021Ravindran Menon D, Li Y, Yamauchi T, Osborne DG, Vaddi PK, Wempe MF, Zhai Z, Fujita M.transcribed with total RNA (500–1000 ng per sample) using Reverse Transcription Kit (Promega, Cat: A3500, Madison, WI, USA) or iScript cDNA synthesis kit (Bio-Rad, Cat: 1708891, Hercules, CA, USA). Quantitative PCR

(146) A Novel Sesquiterpene Lactone Xanthatin-13-(pyrrolidine-2-carboxylic acid) Isolated from Burdock Leaf Up-Regulates Cells’ Oxidative Stress Defense PathwayAntioxidants (Basel)October 14, 2021Yanis A. Idres, Didier Tousch, Guillaume Cazals, Aurélien Lebrun, Sarah Naceri, Luc P. R. Bidel, Patrick PoucheretcDNA synthesis, 1ng of RNA was reverse-transcribed using the Promega Reverse transcription system Ref A3500 (Promega, USA). PCR amplification was then performed using specific primers for SOD, GPx, and G6PD.

(147) Elimination of vision-guided target attraction in Aedes aegypti using CRISPRCurrent BiologySeptember 27, 2021Yinpeng Zhan, Diego Alonso San Alonso San Alberto, Claire Rusch, Jeffrey A. Riffell, Craig MontellAssays,Critical Commercial Assays,Critical Commercial Assays Reverse Transcription System,Promega,Cat#A3500 Infusion HD cloning kit,Clontech,Cat#638909 Plasmid DNA Mini-Prep Kit,Epoch Life Science,Cat#2160250 ZymoPURE

(148) Feasibility of cRGD conjugation at 5â€²-antisense strand of siRNA by phosphodiester linkage extensionMolecular Therapy - Nucleic AcidsSeptember 3, 2021Xinyang Zhou, Yufei Pan, Lijia Yu, Jing Wu, Zheng Li, Huantong Li, Zhu Guan, Xinjing Tang, Zhenjun Yangwith the TRIzol reagent (Invitrogen, USA) and then reversed to cDNA by Reverse Transcription System A3500 (Promega). cDNA was then mixed with forward and reverse primers of BRAFV600E and housekeeping gene β-actin

(149) Anti-Cancer Effects of Cyclic Peptide ALOS4 in a Human Melanoma Mouse ModelInternational journal of molecular sciencesSeptember 3, 2021Levi B, Yacobovich S, Kirby M, Becker M, Agranyoni O, Redko B, Gellerman G, Pinhasov A, Koman I, Nesher E.total of 1 μg of total RNA was reverse-transcribed using a reverse transcription system (Promega, Cat#A3500, Madison, WI, USA). The master mix for cDNA synthesis insisted of 10× Reverse Transcription buffer,

(150) Intravesical CD74 and CXCR4, macrophage migration inhibitory factor (MIF) receptors, mediate bladder painPLOS ONEAugust 23, 2021Shaojing Ye, Fei Ma, Dlovan F. D. Mahmood, Katherine L. Meyer-Siegler, Raymond E. Menard, David E. Hunt, Lin Leng, Richard Bucala, Pedro L. VeraScientific). 1 μg of RNA was reverse transcribed to cDNA using a commercial reverse transcription system (A3500, Promega, Madison, WI). SYBR green (330509, Qiagen Scientific) was utilized with primers (Qiagen, Germantown,

(151) Phenotype and Genotype Study of Chinese POMT2-Related α-DystroglycanopathyFrontiers in GeneticsAugust 3, 2021Xiao-Yu Chen, Dan-Yu Song, Li Jiang, Dan-Dan Tan, Yi-Dan Liu, Jie-Yu Liu, Xing-Zhi Chang, Guo-Gang Xing, Tatsushi Toda, Hui Xiongusing TRIzol. cDNA was synthesized using 1 μg of RNA with a reverse transcription system kit (Promega, A3500, United States) and specific primers, including primers upstream in exon 17 (forward 5′-GGTCAATGACACAGATTTCCGA-3′)

(152) Computational and Mass Spectrometry-Based Approach Identify Deleterious Non-Synonymous Single Nucleotide Polymorphisms (nsSNPs) in JMJD6Molecules (Basel, Switzerland)July 31, 2021Gong T, Yang L, Shen F, Chen H, Pan Z, Zhang Q, Jiang Y, Zhong F, Yang P, Zhang Y.(Invitrogen) and used for first strand cDNA synthesis with the Reverse Transcription System (Promega, A3500). Quantitation of all the gene transcripts was done by qPCR using a Power SYBR Green PCR Master Mix

(153) Trehalose in pine wood nematode participates in DJ3 formation and confers resistance to low-temperature stressBMC GenomicsJuly 9, 2021Qiaoli Chen, Ruizhi Zhang, Danlei Li, Feng Wang, Shengwei Jiang, Jianan Wangextracted using TRIzol [41]. The Promega AMV reverse transcription system was used (Promega, USA, cat. no. A3500) to obtain the first chain of cDNA. One putative TPS-encoding genes, one putative TPP-encoding gene

(154) MicroRNA-181b Serves as a Circulating Biomarker and Regulates Inflammation in Heart FailureDisease MarkersJuly 1, 2021Hongxiao Yang, Lina Shan, Yunan Gao, Lin Li, Guifen Xu, Bin Wang, Xiaoxue Yin, Chengfang Gao, Jiaren Liu, Wei Yangspectrophotometer (Thermo Scientific). Reverse transcription was with a reverse transcription kit (Promega Cat. #A3500) (Table 2). According to the manufacturer's protocol, real-time PCR analysis was performed using the

(155) AMOTL2‑knockdown promotes the proliferation, migration and invasion of glioma by regulating β‑catenin nuclear localizationOncology reportsJuly 1, 2021Chen X, Lu Y, Guo G, Zhang Y, Sun Y, Guo L, Li R, Nan Y, Yang X, Dong J, et al.Thermo Fisher Scientific, Inc.) and reverse-transcribed using GoTaq® Reverse Transcription system (cat. no. A3500; Promega Corporation), according to the manufacturer's instructions. Moreover, 2X SYBR Green qPCR Master

(156) Long non‑coding RNA B3GALT5‑AS1 contributes to the progression of gastric cancer via interacting with CSNK2A1Experimental and Therapeutic MedicineJune 30, 2021Ping Wang, Guang-Bin Sun, Guang-Xian Dou, Bai-Qing WangFisher Scientific, Inc.). Following reverse transcription using AMV Reverse Transcription kit (cat. no. A3500; Promega Corporation), this reaction was performed at 25˚C for 5 min, 50˚C for 20 min, and then 75˚C

(157) Using the dCas9-KRAB system to repress gene expression in hiPSC-derived NGN2 neuronsSTAR protocolsJune 18, 2021Li A, Cartwright S, Yu A, Ho SM, Schrode N, Deans PJM, Matos MR, Garcia MF, Townsley KG, Zhang B, et al.(Invitrogen, #4392938). Alternatively, Reverse transcribe RNA into cDNA using the Promega RT system (Promega, #A3500). Run samples on the Applied Biosystems StepOnePlus Real-Time PCR machine using SYBR Select Master Mix

(158) Transcriptome-Wide Identification and Quantification of Caffeoylquinic Acid Biosynthesis Pathway and Prediction of Its Putative BAHDs Gene Complex in A. spathulifoliusInternational Journal of Molecular SciencesJune 13, 2021Sivagami-Jean Claude, Sunmi Park, Seon-Joo Parkwith the HQT genes to quantify (Table S2). cDNA was synthesized by the Reverse Transcription System (A3500, Promega (product made in USA), Yuseong-gu, Daejeon, Korea), and the final 10 µL product was incubated

(159) Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infectionAntiviral researchJune 1, 2021Ciccosanti F, Di Rienzo M, Romagnoli A, Colavita F, Refolo G, Castilletti C, Agrati C, Brai A, Manetti F, Botta L, et al.SARS-CoV-2 RT-PCR Kit 1.0 (Altona Diagnostic GmbH) or by using a reverse transcription kit (Promega, A3500) cDNA synthesis and Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific, K0253), as previously

(160) Downregulation of SOX9 suppresses breast cancer cell proliferation and migration by regulating apoptosis and cell cycle arrestOncology LettersMay 6, 2021Yu-Feng Wang, Hui-Feng Dang, Xu Luo, Qian-Qian Wang, Chen Gao, Ying-Xia Tianreverse transcribed to cDNA using the Reverse transcription system kit (Promega Corporation; cat. no. A3500), according to the manufacturer's instructions. qPCR analysis was performed using SYBR Master Mixture

Be the First to Write a Review!