Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(181) Efficacy of a novel double-controlled oncolytic adenovirus driven by the Ki67 core promoter and armed with IL-15 against glioblastoma cellsCell & BioscienceDecember 1, 2020Qing Zhang, Junwen Zhang, Yifu Tian, Guidong Zhu, Sisi Liu, Fusheng Liu(Applied Biosystems). cDNA synthesis was performed using the Reverse Transcription System Kit (Promega A3500) according to the manufacturer’s instruction. The codon-optimized IL-15 with the following primers:

(182) TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathyInflammation research : official journal of the European Histamine Research Society ... [et al.]December 1, 2020Zeng H, Qi X, Xu X, Wu Y.SuperMix for qPCR and AceQ qPCR SYBR Green Master Mix were obtained from Vazyme (Vazyme, Nanjing, China); A3500 Reverse Transcription System was obtained from Promega (Promega, Madison, USA); FITC anti-mouse F4/80,

(183) A new method for functional analysis of plastid EMBRYO-DEFECTIVE PPR genes by efficiently constructing cosuppression lines in ArabidopsisPlant MethodsDecember 1, 2020Jingli Chen, Haojie Zhu, Jirong Huang, Weihua Huangwere conducted according to the manufacturer’s instructions (Promega Reverse Transcription System, A3500). PCR was performed using the gene-specific primers that are listed in Additional file 4 and according

(184) m6A Regulates Liver Metabolic Disorders and Hepatogenous DiabetesGenomics, Proteomics & BioinformaticsNovember 5, 2020Yuhuan Li, Qingyang Zhang, Guanshen Cui, Fang Zhao, Xin Tian, Bao-Fa Sun, Ying Yang, Wei LiCarlsbad, CA), and then reverse-transcribed into cDNA using the Reverse Transcription System (Catalog No. A3500, Promega, Madison, WI). qRT-PCR was performed using SYBR Premix Ex Taq kit (Catalog No. RR420A, TaKaRa,

(185) Transcriptional repression of p21 by EIF1AX promotes the proliferation of breast cancer cellsCell proliferationOctober 1, 2020Li Y, Guo L, Ying S, Feng GH, Zhang Y.extraction, and then total RNA was reverse‐transcribed into cDNA with reverse transcription system (A3500; Promega). Quantitative real‐time reverse transcription polymerase chain reaction (qRT‐PCR) was performed

(186) EGFR activity addiction facilitates anti-ERBB based combination treatment of squamous bladder cancerOncogeneOctober 1, 2020Rose M, Maurer A, Wirtz J, Bleilevens A, Waldmann T, Wenz M, Eyll M, Geelvink M, Gereitzig M, Rüchel N, et al.manufacturer’s instructions as indicated. 1 µg of RNA was used for cDNA reverse transcription by Promega Kit A3500 according to the manufacturer’s instructions (Promega, Mannheim, Germany). Semi-quantitative real-time PCR

(187) EZH2 reduction is an essential mechanoresponse for the maintenance of super-enhancer polarization against compressive stress in human periodontal ligament stem cellsCell death & diseaseSeptember 15, 2020Li Q, Sun X, Tang Y, Qu Y, Zhou Y, Zhang Y.manufacturer’s instruction. First strand cDNAs were synthesized with the Reverse Transcription System (Promega, A3500). Quantitation of all gene transcripts was done by quantitative PCR using Power SYBR Green PCR Master Mix

(188) FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like StateCancersSeptember 10, 2020Blanas A, Zaal A, van der Haar Àvila I, Kempers M, Kruijssen L, de Kok M, Popovic MA, van der Horst JC, J van Vliet S.cDNA synthesis was performed using the Reverse Transcription System Kit (Promega, Madison, WI, USA, A3500), according to the manufacturer’s instructions. Synthesized cDNA samples together with the KAPA SYBR®

(189) A promising iPS-based single-cell cloning strategy revealing signatures of somatic mutations in heterogeneous normal cellsComputational and Structural Biotechnology JournalSeptember 3, 2020Xuexia Miao, Yueying Li, Caihong Zheng, Lifei Wang, Chen Jin, Lei Chen, Shuangli Mi, Weiwei Zhai, Qian-Fei Wang, Jun Caiwas extracted using TRIzol reagent and was converted into cDNA using a Reverse Transcriptase System (A3500, Promega). Polymerase chain reaction (PCR) amplification was carried out for 30 cycles (94 °C, 30 sec;

(190) Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloesEurasian Journal of Veterinary SciencesSeptember 1, 2020Oya Bulut, Gülşah Uyunmaz Saklı, Mustafa Hasöksüz, Irmak Dik, Hasan Hüseyin Hadimli, Mustafa Hititsynthesized from the isolated viral RNA by using Reverse Transcription System synthesis kit (Promega A3500, USA). The cDNAs of the samples (3 μl) were treated with the Master Mix mixture, which included 5 μl

(191) Colon-specific immune microenvironment regulates cancer progression versus rejectionOncoimmunologyJuly 12, 2020Trimaglio G, Tilkin-Mariamé AF, Feliu V, Lauzéral-Vizcaino F, Tosolini M, Valle C, Ayyoub M, Neyrolles O, Vergnolle N, Rombouts Y, et al.and reverse-transcribed with AMV Reverse Transcriptase (Reverse Transcription System – Promega, Cat# A3500). For quantitative (q)PCR, 100ng of DNA or the first cDNA strand were mixed with TaqMan Fast Advanced

(192) The Effects of In Vivo Exposure to Copper Oxide Nanoparticles on the Gut Microbiome, Host Immunity, and Susceptibility to a Bacterial Infection in EarthwormsNanomaterials (Basel)July 9, 2020Elmer Swart, Jiri Dvorak, Szabolcs Hernádi, Tim Goodall, Peter Kille, David Spurgeon, Claus Svendsen, Petra ProchazkovaUSA). Per sample, 250 ng of RNA was reverse transcribed to cDNA using Reverse Transcription System A3500 (Promega, Madison, WI, USA) following the standard protocol supplied by the manufacturer. 2.11. Earthworm

(193) PRR11 and SKA2 promote the proliferation, migration and invasion of esophageal carcinoma cellsOncology lettersJuly 1, 2020Chen J, Yang HM, Zhou HC, Peng RR, Niu ZX, Kang CY.instructions, and 2 µg RNA was reverse transcribed to cDNA using the Reverse Transcription System (A3500, Promega Corporation) according to the manufacturers' instructions. The mRNA level was detected using

(194) Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injuryAging (Albany NY)June 9, 2020Sisi Li, Yechen Lu, Daofang Ding, Zhenzhen Ma, Xiangxin Xing, Xuyun Hua, Jianguang XuTRIzol reagent (Invitrogen, USA), and 1 μg of total RNA from each sample was used to synthesize cDNA (A3500, Promega). Real-time PCR amplification was performed on a Light Cycler480 system (Roche, USA) using

(195) A pilot study of cdc6 as a biomarker for circulating tumor cells in patients with lung cancerJournal of Clinical Laboratory AnalysisJune 1, 2020Cheng An, Guijian Liu, Shi Cheng, Bo Pang, Shipeng Sun, Yaying Zhang, Zhongdai Pan, Xixiong KangTRIZOL reagent (Thermo Fisher Scientific). First strain cDNA was synthesized with Reverse Transcription System A3500 (Promega). PCR was set up with Platinum® Taq DNA Polymerase High Fidelity kit (Thermo Fisher Scientific).

(196) TREM2 suppresses the proinflammatory response to facilitate PRRSV infection via PI3K/NF-κB signalingPLoS pathogensMay 1, 2020Zhu Z, Zhang X, Dong W, Wang X, He S, Zhang H, Wang X, Wei R, Chen Y, Liu X, et al.China). Total RNA (1 μg) was reverse transcribed into cDNA using the Reverse Transcription System (A3500, Promega, USA) in a 10 μL reaction volume following the manufacturer’s protocol. Oligo (dT) 15 primer

(197) CASEIN KINASE2-Dependent Phosphorylation of PHOSPHATE2 Fine-tunes Phosphate Homeostasis in RicePlant PhysiologyMay 1, 2020Fei Wang et al.DNA contamination. cDNA was synthesized from 1 mg of total RNA using the Reverse Transcription System (A3500; Promega). RT-qPCR was performed using SYBR Green I Master (Roche) on the LightCycler 480 RealTime PCR

(198) Smooth-muscle-derived WNT5A augments allergen-induced airway remodelling and Th2 type inflammationScientific reportsApril 21, 2020Koopmans T, Hesse L, Nawijn MC, Kumawat K, Menzen MH, Sophie T Bos I, Smits R, Bakker ERM, van den Berge M, Koppelman GH, et al.normalized accordingly. Equal amounts of cDNA were synthesized using AMV reverse transcriptase (Promega, #A3500) and diluted 15 times with RNAse-free ddH2O. Quantitative real-time PCR was performed on an Illumina

(199) Cocirculation of Four Infectious Bronchitis Virus Lineages in Broiler Chickens in the Eastern Region of Saudi Arabia from 2012 to 2014Veterinary Medicine InternationalMarch 31, 2020Abdullah I. A. Al-Mubarak, Anwar A. G. Al-KubatiSynthesis of cDNA First-strand cDNA synthesis was performed using a Reverse Transcription System (Cat #A3500, Promega, USA) according to the manufacturer's instructions. The reaction was performed in a final volume

(200) Antiproliferative and Antimigration Activities of Fluoro-Neplanocin A via Inhibition of Histone H3 Methylation in Triple-Negative Breast CancerBiomoleculesMarch 31, 2020Woong Sub Byun, Won Kyung Kim, Ji-seong Yoon, Dnyandev B. Jarhad, Lak Shin Jeong, Sang Kook Leemicrogram of total RNA per sample was reverse transcribed using a Reverse Transcription System (Cat. No. A3500; Promega, Madison, WI, USA) according to the manufacturer’s instructions. Real-time PCR was conducted

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