Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) The efficacy of the prime-boost regimen for heterologous infectious bronchitis vaccines mandates the administration of homologous vaccinesVirusDiseaseSeptember 1, 2022Anwar A. G. Al-Kubati, Maged Gomaa Hemida, Abdullah I. A. Al-Mubarakinstructions, the Reverse Transcription (RT) was performed using Reverse Transcription System (Cat # A3500, Promega, USA) using Reverse Transcription System (Cat # A3500, Promega, USA) according to manufacturer

(102) Expression of Key Factors of the Hippo Signaling Pathway in Yak (Bos grunniens) Mammary GlandAnimals (Basel)August 17, 2022Peiyan Du, Bin Lu, Ling Zhao, Ying Yao, Wenjie Qian, Zhen Yu, Yan Cui, Sijiu Yu, Jiangfeng Fan(qRT-PCR) was obtained by reverse transcription of the total RNA using the Reverse Transcription System (A3500, Promega, Madison, WI, USA), according to the manufacturer’s instructions. For relative quantitative

(103) Circ TYMP1 Inhibits Carcinogenesis and Cisplatin Resistance in Ovarian Cancer by Reducing Smad2/3 Phosphorylation via a MicroRNA-182A-3p/TGF1B AxisContrast Media & Molecular ImagingAugust 16, 2022Yang Rao, Wenwen Zhang, Dan Li, Xiao Li, Yaomei Ma, Pengpeng Quthe circ TYMP1, miR-182A-3p, and TGF1B primers, according the Genebank database (Shanghai, China). A3500 reverse transcription device was utilized to convert RNA to cDNA (Promega). Next, real-time qPCR was

(104) Targeting MDM4 as a Novel Therapeutic Approach in Prostate Cancer Independent of p53 StatusCancersAugust 16, 2022Mejía-Hernández JO, Raghu D, Caramia F, Clemons N, Fujihara K, Riseborough T, Teunisse A, Jochemsen AG, Abrahmsén L, Blandino G, et al.generation were performed as previously described [19,25] using a Promega Reverse Transcription System (Cat. A3500, Promega). The resulting cDNA was amplified with 2 μM forward (5′-3′, F) and reverse (5′-3′, R) primers

(105) PRRSV Infection Induces Gasdermin D-Driven Pyroptosis of Porcine Alveolar Macrophages through NLRP3 Inflammasome ActivationJournal of virologyJuly 27, 2022He S, Li L, Chen H, Hu X, Wang W, Zhang H, Wei R, Zhang X, Chen Y, Liu X.reverse transcriptase PCR (qRT-PCR) analysis, cDNA was generated from total RNA (1 μg) with a Reverse Transcription System (A3500, Promega, Madison, WI) in a 10-μL reaction volume, following the manufacturer’s protocol, and analyzed

(106) Low Doses of PFOA Promote Prostate and Breast Cancer Cells Growth through Different PathwaysInternational Journal of Molecular SciencesJuly 18, 2022Aurélie Charazac, Charlotte Hinault, Bastien Dolfi, Solène Hautier, Célia Decondé Le Butor, Frédéric Bost, Nicolas Chevaliertranscription of 1 mg of total RNA into first strand cDNA was done using the reverse transcription system (A3500, Promega® [Charbonnières-les-Bains, France]). Fast SYBR Green master mix (Applied Biosystems® [Asnières

(107) The human amniotic epithelium confers a bias to differentiate toward the neuroectoderm lineage in human embryonic stem cellseLifeJuly 11, 2022Ávila-González D, Portillo W, Barragán-Álvarez CP, Hernandez-Montes G, Flores-Garza E, Molina-Hernández A, Díaz-Martínez NE, Díaz NF.agarose gels. The cDNA was synthesized with Oligo dT using the Reverse Transcription System Kit (Promega, A3500). The qPCR amplification was generated with KAPA SYBR FAST qPCR Master Mix 2× (KM4100) in a Rotor-Gene Q

(108) Electroacupuncture treatment improves motor function and neurological outcomes after cerebral ischemia/reperfusion injuryNeural regeneration researchJuly 1, 2022Li SS, Hua XY, Zheng MX, Wu JJ, Ma ZZ, Xing XX, Ma J, Shan CL, Xu JG.sample, complementary DNA was synthesized from 1 μg of total RNA using the Reverse Transcription System A3500 (Promega, Madison, WI, USA). Quantitative real-time polymerase chain reactions (qPCR) were performed

(109) Targeting the SREBP-1/Hsa-Mir-497/SCAP/FASN Oncometabolic Axis Inhibits the Cancer Stem-like and Chemoresistant Phenotype of Non-Small Cell Lung Carcinoma CellsInternational Journal of Molecular SciencesJune 30, 2022Tung-Yu Tiong, Pei-Wei Weng, Chun-Hua Wang, Syahru Agung Setiawan, Vijesh Kumar Yadav, Narpati Wesa Pikatan, Iat-Hang Fong, Chi-Tai Yeh, Chia-Hung Hsu, Kuang-Tai Kuofrom 1.0 μg of the total RNA by using the oligo dT primer and a reverse transcription system (Cat. #A3500, Promega, Madison, WI, USA) according to the manufacturer’s instructions. A quantitative real-time polymerase chain reaction

(110) Targeted Modulation of Interferon Response-Related Genes with IFN-Alpha/Lambda InhibitionInternational Journal of Molecular SciencesJune 29, 2022Debpali Sur et al.total of 1 μg of total RNA was reverse-transcribed using a reverse-transcription system (Promega, Cat# A3500, Madison, WI, USA). The master mix for cDNA synthesis consisted of 10× reverse-transcription buffer,

(111) Identification of Pyroptosis-Related Gene Signatures and Construction of the Risk Model to Predict BCR in Prostate CancerFrontiers in Molecular BiosciencesJune 23, 2022Miaomiao Wang, Haoran Xia, Qiuxia Yan, Wen Liu, Ming Liu, Xuan Wangvia spectrophotometry. Reverse transcription was conducted using the reverse transcription system (A3500, Promega, America). We used SYBR Green Premix Pro Taq HS qPCR Kit (Accurate Biology) and MA-6000 real-time

(112) Interleukin-10-Modified Adipose-Derived Mesenchymal Stem Cells Prevent Hypertrophic Scar Formation via Regulating the Biological Characteristics of Fibroblasts and InflammationMediators of InflammationJune 21, 2022Fang Xie, Li Teng, Jianjian Lu, Jiajie Xu, Chao Zhang, Liya Yang, Xiaoyang Ma, Minghao ZhaoGen, China) to extract total RNA from tissues and prepared cDNA using a reverse transcription kit (A3500, Promega, USA). As stated in manufacturing instructions of a GoTaq qPCR mix kit (A6006A, Promega, USA),

(113) Hemp Seed Fermented by Aspergillus oryzae Attenuates Lipopolysaccharide-Stimulated Inflammatory Responses in N9 Microglial CellsFoodsJune 9, 2022Zeyuan Wang, Lehao Wu, Dongmei Fu, Yan Zhang, Chunzhi Zhangcells. mRNA was reverse transcribed into complementary DNA (cDNA) by the reverse transcription kit (A3500, Promega, Madison, WI, USA). cDNA was then performed to Real-Time PCR, and the transcripts of iNOS,

(114) Integrating Network Pharmacology and Experimental Validation to Explore the Key Mechanism of Gubitong Recipe in the Treatment of OsteoarthritisComputational and Mathematical Methods in MedicineJune 8, 2022Guang-yao Chen, Xiao-yu Liu, Jing Luo, Xin-bo Yu, Yi Liu, Qing-wen TaoCellTiter 96® AQueous One Solution Cell Proliferation Assay (G3582) and Reverse Transcription System (A3500) were purchased from Promega. HiPure Total RNA Mini Kit (R4111-02) was purchased from Magen. PAGE Gel

(115) A possible new activator of PI3K-Huayu Qutan Recipe alleviates mitochondrial apoptosis in obesity rats with acute myocardial infarctionJournal of cellular and molecular medicineJune 1, 2022Tai H, Tong YJ, Yu R, Yu Y, Yao SC, Li LB, Liu Y, Cui XZ, Kuang JS, Meng XS, et al.Reverse transcription was conducted using 1 µg of total RNA and M‐MLV Reverse Transcriptase Kit (Promega A3500). A 40 µl total reaction system was used in a Thermal Cycler with 96 wells (Applied Biosystems) in accordance

(116) A Novel Estrogen Receptor β Agonist Diminishes Ovarian Cancer Stem Cells via Suppressing the Epithelial-to-Mesenchymal TransitionCancersMay 6, 2022Banerjee A, Cai S, Xie G, Li N, Bai X, Lavudi K, Wang K, Zhang X, Zhang J, Patnaik S, et al.transcription was performed to generate cDNA using the Promega Reverse Transcription System (Promega, Cat. No: A3500) in a 20 µL reaction containing 2 µg of total RNA. A 0.5 µL aliquot of cDNA was amplified by Fast SYBR

(117) Treatment of SARS-CoV-2-induced pneumonia with NAD(+) and NMN in two mouse modelsCell discoveryApril 29, 2022Jiang Y, Deng Y, Pang H, Ma T, Ye Q, Chen Q, Chen H, Hu Z, Qin CF, Xu Z.respectively. RT-qPCR 1.5 μg of total RNA was used for RT-qPCR using Reverse Transcription Kit (Promega, A3500) according to the manufacturer’s protocol. Then, cDNA was diluted by 1:4 and 1 μL diluted cDNA was used

(118) Disrupting the HDAC6-ubiquitin interaction impairs infection by influenza and Zika virus and cellular stress pathwaysCell reportsApril 26, 2022Wang L, Moreira EA, Kempf G, Miyake Y, Oliveira Esteves BI, Fahmi A, Schaefer JV, Dreier B, Yamauchi Y, Alves MP, et al.E0554S FastStart Universal SYBR Green Master (Rox),Roche,Cat# 4913850001 Reverse Transcription System,Promega,Cat#A3500 Proteinase K,Merck,Cat# 3115887001 DAPI,Invitrogen,Cat#D1306 Opti-MEM,Gibco,Cat#31985062 ProLong™ Gold Antifade Mountant

(119) Downregulation of neurodevelopmental gene expression in iPSC-derived cerebral organoids upon infection by human cytomegalovirusiScienceApril 15, 2022O'Brien BS, Mokry RL, Schumacher ML, Pulakanti K, Rao S, Terhune SS, Ebert AD.Technologies. Total RNA was isolated, and reverse transcribed into cDNA using the Promega RT Kit (#A3500). q-RT-PCR was performed using specific primer sequences as outlined in (Table S7). The resulting CT

(120) Melanoma secretion of transforming growth factorâ€Î²2 leads to loss of epidermal AMBRA1 threatening epidermal integrity and facilitating tumour ulceration*British Journal of DermatologyApril 1, 2022I. Cosgarea, A.T. McConnell, T. Ewen, D. Tang, D.S. Hill, M. Anagnostou, M. Elias, R.A. Ellis, A. Murray, L.C. Spender, et al.(Promega, Southampton, UK; Z6011) and reverse transcribed using an AMV Reverse Transcriptase kit (Promega, A3500) or High Capacity Reverse Transcription Kit (ThermoFisher Scientific; 4368814), according to the manufacturer’s

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