Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(581) Involvement of phospholipase D in the low temperature acclimation-induced thermotolerance in grape berryPlant Physiology and BiochemistryDecember 15, 2008Si-Bao Wan, Li Tian, Rong-Rong Tian, Qiu-Hong Pan, Ji-Cheng Zhan, Peng-Fei Wen, Jian-Ye Chen, Ping Zhang, Wei Wang, Wei-Dong Huang

(582) Differential gene expression associated with postnatal equine articular cartilage maturationBMC Musculoskeletal DisordersDecember 1, 2008Michael J Mienaltowski, Liping Huang, Arnold J Stromberg, James N MacLeodinto cDNA using an oligo-dT primer with the Promega Reverse Transcription System (Promega, cat. no. A3500). Quantitative "real-time" PCR (ABI Systems 7900HT Fast Real-Time PCR System, Applied Biosystems, Foster

(583) Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patientsJournal of Translational MedicineDecember 1, 2008Yuanxin Xu, Valerie Theobald, Crystal Sung, Kathleen DePalma, Laura Atwater, Keirsten Seiger, Michael A Perricone, Susan M Richardsreal time RT-PCR and ELISPOT; Qiagen Rneasy Mini Kit (74106, Qiagen), Promega Reverse Transcription Kit (A3500, Promega), and TaqMan Universal Mix (4304437, Applied Biosystems) for RT-PCR. Equipment FACSCalibur

(584) Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injuryWorld Journal of GastroenterologyOctober 14, 2008Jin-Shui Pan, Shao-Zhen He, Hong-Zhi Xu, Xiao-Juan Zhan, Xiao-Ning Yang, Hong-Min Xiao, Hua-Xiu Shi, Jian-Lin RenLiberation Army. TRIzol reagent (Cat No. 15596-026) came from Invitrogen Corporation. RT-PCR kits (Cat No. A3500), Taq DNA polymerase, and 100 bp DNA ladder were bought from the Promega Corporation. Equipments G ava

(585) Mucosal tolerance to E-selectin provides protection against cerebral ischemia–reperfusion injury in ratsJournal of NeuroimmunologySeptember 4, 2008Wu Yun, Liang qing-cheng, Yang Lei, Miao Jia-yin

(586) Novel mutations of the SPG11 gene in hereditary spastic paraplegia with thin corpus callosumJournal of the Neurological SciencesJuly 31, 2008Shu-sheng Liao, Lu Shen, Juan Du, Guo-huan Zhao, Xiao-yi Wang, Yi Yang, Zi-quan Xiao, Yi Yuan, Hong Jiang, Nan Li, He-dong Sun, Jun-ling Wang, Chun-yu Wang, Ya-fang Zhou, Xiao-yun Mo, Kun Xia, Bei-sha Tang

(587) Prokaryotic expression, polyclonal antibody preparation of the stilbene synthase gene from grape berry and its different expression in fruit development and under heat acclimationPlant Physiology and BiochemistryJuly 14, 2008Wei Wang, Si-Bao Wan, Ping Zhang, Hui-Ling Wang, Ji-Cheng Zhan, Wei-Dong Huang

(588) Enhanced expression and activation of pro-inflammatory transcription factors distinguish aneurysmal from atherosclerotic aorta: IL-6- and IL-8-dominated inflammatory responses prevail in the human aneurysmClinical science (London, England : 1979)June 1, 2008Lindeman JH, Abdul-Hussien H, Schaapherder AF, Van Bockel JH, Von der Thüsen JH, Roelen DL, Kleemann R.THE FINAL VERSION - see doi:10.1042/CS20070352 TP instructions. Then, cDNA was prepared using kit #A3500 (Promega, Leiden, The Clinical Science Immediate Publication. Published on 13 Dec 2007 as manuscript

(589) Methamphetamine alters blood brain barrier permeability via the modulation of tight junction expression: Implication for HIV-1 neuropathogenesis in the context of drug abuseBrain researchApril 8, 2008Mahajan SD, Aalinkeel R, Sykes DE, Reynolds JL, Bindukumar B, Adal A, Qi M, Toh J, Xu G, Prasad PN, et al.transcribed to cDNA using the reverse transcriptase kit from Promega (Promega Inc, Madison, WI; Cat # A3500). Relative abundance of each mRNA species is quantitated using real time quantitative PCR using specific

(590) Silencing of herg gene by shRNA inhibits SH-SY5Y cell growth in vitro and in vivoEuropean journal of pharmacologyJanuary 28, 2008Zhao J, Wei XL, Jia YS, Zheng JQ.

(591) Aging Up-Regulates Expression of Inflammatory Mediators in Mouse Adipose TissueThe Journal of ImmunologyOctober 1, 2007Dayong Wu, Zhihong Ren, Munkyong Pae, Weimin Guo, Xuelin Cui, Alfred H. Merrill, Simin Nikbin Meydani260 nm. All samples had a ratio of 260/280 nm at ⬎1.8. RNA was reverse transcribed into cDNA using a A3500 kit (Promega) reverse transcription system, and the cDNA was used to conduct TaqMan real-time quantitative PCR

(592) Rapid CD8⁺T Cell Repertoire Focusing and Selection of High-Affinity Clones into Memory Following Primary Infection with a Persistent Human Virus: Human CytomegalovirusThe Journal of ImmunologySeptember 1, 2007Elizabeth K. Day, Andrew J. Carmichael, Ineke J. M. ten Berge, Edward C. P. Waller, J. G. Patrick Sissons, Mark R. Willsextraction kits (Qiagen). First-strand cDNA synthesis was conducted using the Reverse Transcriptase A3500 System kit (Promega). PCR was performed, as previously described (23), using a panel of 39 V␤ family primers

(593) Cloning of Phospholipase D from Grape Berry and Its Expression under Heat AcclimationJ Biochem Mol BiolJuly 31, 2007Si-Bao Wan, Wei Wang, Peng-Fei Wen, Jian-Ye Chen, Wei-Fu Kong, Qiu-Hong Pan, Ji-Cheng Zhan, Li Tian, Hong-Tao Liu, Wei-Dong HuangFirst-stand cDNA was synthesized in a 20 µl reaction mixture with the Reverse Transcription System A3500 (Promega) according to the protocol provided by the manufacturer, using 1 µg total RNA isolated from

(594) Robust analysis of 5′-transcript ends: a high-throughput protocol for characterization of sequence diversity of transcription start sitesNature ProtocolsJuly 1, 2007Malali Gowda, Huameng Li, Guo-Liang WangPolyethylene glycol 8000 (Sigma-Aldrich, cat. no. 81268) . Reverse transcriptase kit (Promega, cat. no. A3500) . M-280 streptavidin beads slurry (Dynal, cat. no. 18090-019) . Trizol (Invitrogen, cat. no. 18068-015) .

(595) Acidosis-Sensing Glutamine Pump SNAT2 Determines Amino Acid Levels and Mammalian Target of Rapamycin Signalling to Protein Synthesis in L6 Muscle CellsJournal of the American Society of NephrologyMay 1, 2007Kate Evans, Zeerak Nasim, Jeremy Brown, Heather Butler, Samira Kauser, Hélène Varoqui, Jeffrey D. Erickson, Terence P. Herbert, Alan Bevingtonspectrophotometry at 260 nm. RNA was reverse-transcribed using an AMV Reverse Transcription System (Promega A3500, Madison, WI) and was amplified by PCR using the following gene-specific primers: Rat SNAT1 (28) (493-bp

(596) LAZY1 controls rice shoot gravitropism through regulating polar auxin transportCell ResearchMay 1, 2007Peijin Li, Yonghong Wang, Qian Qian, Zhiming Fu, Mei Wang, Dali Zeng, Baohua Li, Xiujie Wang, Jiayang Liof total RNA was treated with DNase I and used for complementary DNA synthesis with RT kit (Cat. No. A3500, Promega). 5′- or 3′RACE of LA1 was carried out by using a SMART™ RACE cDNA Amplification Kit according

(597) IFN Regulatory Factor-2 Regulates Macrophage Apoptosis through a STAT1/3- and Caspase-1-Dependent MechanismThe Journal of ImmunologyMarch 15, 2007Natalia Cuesta, Quan M. Nhu, Enrique Zudaire, Swamy Polumuri, Frank Cuttitta, Stefanie N. Vogeltotal of 1 ␮g of RNA was used for oligo(dT)-primed cDNA synthesis (Promega reverse transcription system A3500). Real-time PCR was performed on an ABI Prism 7900HT Sequence Detection System (Applied Biosystems)

(598) Expression of Sonic hedgehog gene, interleukin-1β, tumor necrosis factor-α in gastric epithelium of mongolian gerbils afterH pyloriinoculationWorld Chinese Journal of DigestologyJanuary 1, 2007Zhong-Wu Li, Lu-Ying Liu, Meng-Meng Tian, Wei-Cheng You, Ji-You LiA)试剂盒说明提取总R N A; 紫外分光光度些结果提示, Shh基因可能在萎缩性胃炎及肠上计测定总RNA浓度及纯度; 应用逆转录试剂盒皮化生病变中发挥着重要作用. 另外, Dimmler (A3500, Promega, USA)逆转录合成cDNA. 具体 et al 在体外实验发现, 低pH值环境促进Shh基的步骤如下: 取总RNA 0.5 g/L, 在70℃条件下使因在胃癌细胞系23132中的表达;

(599) Prognostic significance of cyclooxygenase-2 (COX-2) expression in patients with surgically resectable adenocarcinoma of the oesophagusBMC CancerDecember 1, 2006Pradeep Bhandari, Adrian C Bateman, Raj L Mehta, Bernard SF Stacey, Penny Johnson, Ian A Cree, Federica Di Nicolantonio, Praful Patelreverse-transcribed by using the Promega reverse transcription system (Promega, Southampton, UK, Cat # A3500). The resulting c-DNA was amplified by real-time quantitative PCR on a Biorad iCycler instrument (BioRad

(600) Identification and analysis of YELLOW protein family genes in the silkworm, Bombyx moriBMC GenomicsDecember 1, 2006Ai-Hua Xia, Qing-Xiang Zhou, Lin-Lin Yu, Wei-Guo Li, Yong-Zhu Yi, Yao-Zhou Zhang, Zhi-Fang Zhangwere used to synthesize cDNAs according to the Reverse Transcription kit's protocol (Promega Cat.: A3500). Using the reverse transcription products as the templates, 30 cycles of amplification were performed

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