Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(561) A Single Active Trehalose-6-P Synthase (TPS) and a Family of Putative Regulatory TPS-Like Proteins in ArabidopsisMolecular PlantDecember 9, 2009Lies Vandesteene, Matthew Ramon, Katrien Le Roy, Patrick Van Dijck, Filip Rolland

(562) Decreased apolipoprotein A5 is implicated in insulin resistance-related hypertriglyceridemia in obesityAtherosclerosisDecember 3, 2009Xian-sheng Huang, Shui-Ping Zhao, Min Hu, Lin Bai, Qian Zhang, Wang Zhao

(563) Distribution of resveratrol and stilbene synthase in young grape plants ( Vitis vinifera L. cv. Cabernet Sauvignon) and the effect of UV-C on its accumulationPlant Physiology and BiochemistryDecember 1, 2009Wei Wang, Ke Tang, Hao-Ru Yang, Peng-Fei Wen, Ping Zhang, Hui-Ling Wang, Wei-Dong Huang

(564) Expression profile of multiple aquaporins in human gastric carcinoma and its clinical significanceBiomedicine & PharmacotherapyDecember 1, 2009Lizong Shen, Zhengcai Zhu, Yiming Huang, Yongqian Shu, Maocai Sun, Hao Xu, Guoqiang Zhang, Renhua Guo, Wei Wei, Wenxi Wu

(565) Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IBiological Procedures OnlineDecember 1, 2009Alina Rodríguez-Mallon, Yamilet Cárdenas, Juana María Lugo, Aymé Oliva, Antonio Morales, Mario Pablo EstradaZ5110 • PolyATract: mRNA Isolation System II, Promega Z5200 • Reverse Transcription System, Promega A3500 • PCR Master Mix, Promega M7505 • QIAquick Gel Extraction Kit, QIAGEN 28704 • pGEM®-T Easy Vector System I

(566) Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissuesBMC Plant BiologyDecember 1, 2009Brian W Kram, Wayne W Xu, Clay J Carterreverse transcribed (0.1 μg per tissue) with Promega's (Madison, WI, USA) Reverse Transcription System (A3500), and PCR was performed with GoTaq Green Master Mix (Promega, M7122). Negative control reactions using

(567) Transcriptional profiling differences for articular cartilage and repair tissue in equine joint surface lesionsBMC Medical GenomicsDecember 1, 2009Michael J Mienaltowski, Liping Huang, David D Frisbie, C Wayne McIlwraith, Arnold J Stromberg, Arne C Bathke, James N MacLeodinto cDNA using an oligo-dT primer with the Promega Reverse Transcription System (Promega, cat. no. A3500). Quantitative "real-time" PCR (7500 Sequence Detection and 7900 HT Fast Real-Time PCR Systems, Applied

(568) Human testis-specific PDHA2 gene: Methylation status of a CpG island in the open reading frame correlates with transcriptional activityMolecular Genetics and MetabolismNovember 11, 2009Ana Pinheiro, Inês Faustino, Maria João Silva, Joaquina Silva, Rosália Sá, Mário Sousa, Alberto Barros, Isabel Tavares de Almeida, Isabel Rivera

(569) Shear stress magnitude and duration modulates matrix composition and tensile mechanical properties in engineered cartilaginous tissueBiotechnology and BioengineeringNovember 1, 2009Christopher V. Gemmiti, Robert E. Guldberganalysis (NanoDrop_Technologies 2005). cDNA was generated according to manufacturer's instructions (A3500 Reverse Transcription Kit; Promega, Madison, WI) using 1-μg RNA per sample. Samples were frozen at -20°C

(570) A role for GATA-2 in transition to an aggressive phenotype in prostate cancer through modulation of key androgen-regulated genesOncogeneOctober 29, 2009Böhm M, Locke WJ, Sutherland RL, Kench JG, Henshall SM.Supplementary Material and methods. Quantitative real-time PCR Total RNA was converted to cDNA using the A3500 Reverse Transcription System (Promega). Quantitative real-time PCR was carried out on 20 ng of RNA using

(571) Circulating microRNA-1 as a potential novel biomarker for acute myocardial infarctionBiochemical and Biophysical Research CommunicationsOctober 18, 2009Jing Ai, Rong Zhang, Yue Li, Jielin Pu, Yanjie Lu, Jundong Jiao, Kang Li, Bo Yu, Zhuqin Li, Rongrong Wang, Lihong Wang, Qiang Li, Ning Wang, Hongli Shan, Zhongyu Li, Baofeng Yang

(572) Neuroprotective effects of genistein and folic acid on apoptosis of rat cultured cortical neurons induced by beta-amyloid 31-35British Journal of NutritionSeptember 14, 2009Huan-Ling Yu, Li Li, Xiao-Hong Zhang, Li Xiang, Jie Zhang, Jin-Fang Feng, Rong Xiaopurified using Trizol (Invitrogen, Carlsbad, CA, USA) and reverse-transcribed using an RT kit (no. A3500; Applied Promega, Madison, WI, USA). mRNA encoding bax, bcl-2, caspase-3, p53 and b-actin (used as an invariant

(573) Incidence, molecular epidemiology and clinical presentations of human metapneumovirus; assessment of its importance as a diagnostic screening targetJournal of Clinical VirologySeptember 11, 2009Eleanor Gaunt, E. Carol McWilliam-Leitch, Kate Templeton, Peter Simmonds

(574) Changes of resveratrol and antioxidant enzymes during UV-induced plant defense response in peanut seedlingsJournal of Plant PhysiologyJuly 22, 2009Ke Tang, Ji-Cheng Zhan, Hao-Ru Yang, Wei-Dong Huang

(575) Oleate induces transdifferentiation of chicken fibroblasts into adipocyte-like cellsComparative Biochemistry and Physiology, Part AMay 19, 2009Shuang Liu, Li Wang, Ning Wang, Yuxiang Wang, Hui Shi, Hui Li

(576) Regulation of deoxycholate induction of CXCL8 by the adenomatous polyposis coli gene in colorectal cancerInternational journal of cancerMay 15, 2009Rial NS, Lazennec G, Prasad AR, Krouse RS, Lance P, Gerner EW.protocol. Samples were quantified then reverse transcribed using Promega Reverse Transcription System (A3500) according to manufacturer’s instructions. The product served as a template (at 100 ng/μL) for the PCR reactions

(577) Clinical trial of doxycycline for matrix metalloproteinase-9 inhibition in patients with an abdominal aneurysm: doxycycline selectively depletes aortic wall neutrophils and cytotoxic T cellsCirculationApril 28, 2009Lindeman JH, Abdul-Hussien H, van Bockel JH, Wolterbeek R, Kleemann R.Netherlands) and glass beads according to the manufacturer’s instructions. Copy DNA was prepared with the A3500 kit (Promega, Leiden, the Netherlands), and quantitative real-time polymerase chain reaction analysis

(578) Functional characterization of p53 in nasopharyngeal carcinoma by stable shRNA expressionInternational journal of oncologyApril 1, 2009Sun Y, Yi H, Yang Y, Yu Y, Ouyang Y, Yang F, Xiao Z, Chen Z.manufacturer's instructions. Two microgram RNA per sample was reversetranscribed into first-strand cDNA using A3500 reverse transcription system (Promega, USA) in a standard protocol with random oligo(dT) primers. RT-PCR

(579) Mechanisms of alpha1-adrenoceptor mediated QT prolongation in the diabetic rat heartLife sciencesFebruary 13, 2009Chen L, Wang L, Xu B, Ni G, Yu L, Han B, Yu X, Wang K, Lai Y, Zhou S, et al.

(580) Olig2-Induced Neural Stem Cell Differentiation Involves Downregulation of Wnt Signaling and Induction of Dickkopf-1 ExpressionPLoS ONEDecember 18, 2008Sung-Min Ahn, Kyunghee Byun, Deokhoon Kim, Kiyoung Lee, Jong Shin Yoo, Seung U. Kim, Eek-hoon Jho, Richard J. Simpson, Bonghee Lee(Qiagen), pooled, and subjected to first-strand cDNA synthesis using Reverse Transcription System (Promega, A3500) according to the manufacturer’s instructions. Amplification was carried out at 94uC for 2 min, followed

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