Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(601) Fenofibrate reduces atherogenesis in ApoE*3Leiden mice: evidence for multiple antiatherogenic effects besides lowering plasma cholesterolArteriosclerosis, thrombosis, and vascular biologyOctober 1, 2006Kooistra T, Verschuren L, de Vries-van der Weij J, Koenig W, Toet K, Princen HM, Kleemann R.Netherlands) and glass beads according to the manufacturer’s instructions. Then, cDNA was prepared using kit #A3500 (Promega, Leiden, The Netherlands) for real-time polymerase chain reaction (RT-PCR) analysis. The mastermix

(602) Rapamycin-Mediated Enrichment of T Cells with Regulatory Activity in Stimulated CD4⁺ T Cell Cultures Is Not Due to the Selective Expansion of Naturally Occurring Regulatory T Cells but to the Induction of Regulatory Functions in Conventional CD4⁺ T CellsThe Journal of ImmunologyJuly 15, 2006Danila Valmori, Valeria Tosello, Naira E. Souleimanian, Emmanuelle Godefroy, Luigi Scotto, Yu Wang, Maha Ayyoubusing RNeasy Mini Kit (Qiagen). cDNA synthesis was performed using Promega Reverse Transcription System A3500 (Promega), and cDNA integrity was tested by amplification of RPL32 in a 35-cycle PCR. Semiquantitative

(603) Evidence for reduced bone formation surface relative to bone resorption surface in female femoral fragility fracture patientsBoneJune 28, 2006Helen Tsangari, David M. Findlay, Andrew C.W. Zannettino, Beiqing Pan, Julia S. Kuliwaba, Nicola L. Fazzalari

(604) Identification of two Melan-A CD4 + T cell epitopes presented by frequently expressed MHC class II allelesClinical ImmunologyMay 12, 2006Emmanuelle Godefroy, Luigi Scotto, Naira E. Souleimanian, Gerd Ritter, Lloyd J. Old, Francine Jotereau, Danila Valmori, Maha Ayyoub

(605) Liver X receptor agonist TO-901317 upregulates SCD1 expression in renal proximal straight tubuleAmerican journal of physiology. Renal physiologyMay 1, 2006Zhang Y, Zhang X, Chen L, Wu J, Su D, Lu WJ, Hwang MT, Yang G, Li S, Wei M, et al.␮M of random primers according to the manufacturer’s protocol (Promega reverse transcription system A3500). cDNA samples were then used as templates for quantitative PCR. Primers used were 5⬘-AAG TTC GCA CTG

(606) Isolation of CD4+CD25+ regulatory T cells for clinical trialsBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow TransplantationMarch 1, 2006Hoffmann P, Boeld TJ, Eder R, Albrecht J, Doser K, Piseshka B, Dada A, Niemand C, Assenmacher M, Orsó E, et al.

(607) Expression of CD28 and cytotoxic T lymphocyte antigen 4 at the maternal–fetal interface in women with unexplained pregnancy lossInternational Journal of Gynecology and ObstetricsJanuary 25, 2006Xipeng Wang, Zhengwen Ma, Yan Hong, Peihua Lu, Qide Lin

(608) Effect of salicylic acid on phenylpropanoids and phenylalanine ammonia-lyase in harvested grape berriesPostharvest Biology and TechnologyDecember 19, 2005Jian-Ye Chen, Peng-Fei Wen, Wei-Fu Kong, Qiu-Hong Pan, Ji-Cheng Zhan, Jing-Ming Li, Si-Bao Wan, Wei-Dong Huang

(609) Cancer cell adaptation to chemotherapyBMC CancerDecember 1, 2005Federica Di Nicolantonio et al.reverse-transcribed using the Promega reverse transcription system (Promega, Southampton, UK; Cat No. A3500) including 8 μl RNA, 0.5 μg random primers, 20 units of recombinant RNasin® ribonuclease inhibitor and

(610) Chinese soft-shelled turtle egg powder lowers serum cholesterol, increases faecal neutral steroids and bile acid excretion, and up-regulates liver cytochrome P450 mRNA level in ratsBritish Journal of NutritionSeptember 24, 2005Yu Huanling, Li Yong, Wang Junbo, Zheng Liping, Yan WeixingTrizol (Invitrogen, Carlsbad, CA, USA) and reverse-transcribed using a Reverse Transcriptase kit (#A3500; Applied Promega, Madison, WI, USA). RNA encoding 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase, cholesterol 7a-hydroxylase

(611) Differential expression of survivin-2B and survivin-DeltaEx3 is inversely associated with disease relapse and patient survival in non-small-cell lung cancer (NSCLC)Lung CancerSeptember 1, 2005Xiang Ling, Jie Yang, DongFeng Tan, Nithya Ramnath, Tallal Younis, Brian N. Bundy, Harry K. Slocum, LiLy Yang, Muxiang Zhou, Fengzhi Lidetermination of RNA concentration. RT reaction was performed using Reverse Transcription System (#A3500, Promega, Madison, WI) following the manufacturer’s instruction. Briefly, total 20 µl of RT reactions

(612) A peripheral circulating compartment of natural naive CD4+ TregsJournal of Clinical InvestigationJuly 1, 2005Danila Valmori, Andrea Merlo, Naira E. Souleimanian, Charles S. Hesdorffer, Maha Ayyoubusing RNeasy Mini Kit (QIAGEN). cDNA synthesis was performed using Promega Reverse Transcription System A3500 (Promega), and cDNA integrity was tested by amplification of β-actin in a 35-cycle PCR reaction. Semiquantitative

(613) Salicylic acid induced the expression of phenylalanine ammonia-lyase gene in grape berryPlant ScienceJune 20, 2005Peng-Fei Wen, Jian-Ye Chen, Wei-Fu Kong, Qiu-Hong Pan, Si-Bao Wan, Wei-Dong Huang

(614) ER stress signaling by regulated splicing: IRE1/HAC1/XBP1MethodsMarch 4, 2005Sung Hoon Back, Martin Schröder, Kyungho Lee, Kezhong Zhang, Randal J. Kaufman

(615) Activation of STAT3 signaling in human stomach adenocarcinoma drug-resistant cell line and its relationship with expression of vascular endothelial growth factorWorld Journal of GastroenterologyFebruary 14, 2005Li-Fen Yu, Ying Cheng, Min-Min Qiao, Yong-Ping Zhang, Yun-Lin Wustandard were purchased from Invitrogen (Carlsbad, California. USA). AMV reverse transcription system (A3500), Taq DNA polymerase, dNTP, PCR marker and EMSA kit were purchased from Promega (Madison, WI, USA).

(616) Effect of Low Dose Atorvastatin Versus Diet-Induced Cholesterol Lowering on Atherosclerotic Lesion Progression and Inflammation in Apolipoprotein E*3–Leiden Transgenic MiceArteriosclerosis, Thrombosis, and Vascular BiologyJanuary 1, 2005Lars Verschuren, Robert Kleemann, Erik H. Offerman, Alexander J. Szalai, Sjef J. Emeis, Hans M. G. Princen, Teake Kooistracontrol mice are below the detection limit of the ELISA. Aortic mRNA Expression mRNA and cDNA (kit A3500; Promega) was prepared from n⫽5 aortas per experimental group.16 RT-PCR was performed using the RT-PCR–mastermix

(617) Adenoviral transfer of human interleukin-10 gene in lethal pancreatitisWorld Journal of GastroenterologyOctober 15, 2004Zi-Qian Chen, Yao-Qing Tang, Yi Zhang, Zhi-Hong Jiang, En-Qiang Mao, Wei-Guo Zou, Ruo-Qing Lei, Tian-Quan Han, Sheng-Dao ZhangAliquots of 5 µg of total RNA were reversetranscribed by using a first-strand cDNA synthesis kit (Promega A3500). The cDNA was then amplified by polymerase chain reaction using primers specific for hIL-10 or TNF-α

(618) Exercise and Myocyte Enhancer Factor 2 Regulation in Human Skeletal MuscleDiabetesMay 1, 2004Sean L. McGee, Mark HargreavesAND MEF-2 REGULATION stranded cDNA was synthesized using an AMV Reverse Transcriptase Kit (Promega A3500; Promega, Madison, WI). Forward and reverse primers complementary to the human GLUT4 gene (GenBank NM

(619) An immunodominant SSX-2–derived epitope recognized by CD4+ T cells in association with HLA-DRJournal of Clinical InvestigationApril 15, 2004Maha Ayyoub et al.Promega secretion, T cells were stimulated in the absence or the presence of Reverse Transcription System A3500 (Promega Corp., Madison, peptides at the described dose for 4–6 hours. Where indicated, Wisconsin, USA).

(620) An immunodominant SSX-2-derived epitope recognized by CD4+ T cells in association with HLA-DRThe Journal of clinical investigationApril 1, 2004Ayyoub M, Hesdorffer CS, Montes M, Merlo A, Speiser D, Rimoldi D, Cerottini JC, Ritter G, Scanlan M, Old LJ, et al.Promega secretion, T cells were stimulated in the absence or the presence of Reverse Transcription System A3500 (Promega Corp., Madison, peptides at the described dose for 4–6 hours. Where indicated, Wisconsin, USA).

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