Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(481) Improved Nutritive Quality and Salt Resistance in Transgenic Maize by Simultaneously Overexpression of a Natural Lysine-Rich Protein Gene, SBgLR, and an ERF Transcription Factor Gene, TSRF1International Journal of Molecular SciencesApril 29, 2013Meizhen Wang, Chen Liu, Shixue Li, Dengyun Zhu, Qian Zhao, Jingjuan Yustrand synthesis according to the manufacturer’s protocol of Reverse Transcription System (Promega A3500, Madison, WI, USA). For quantitative real-time PCR amplification, 100 ng cDNA was used as template in

(482) Characterization and Functional Analysis of the Potato Pollen-Specific Microtubule-Associated Protein SBgLR in TobaccoPLoS ONEMarch 25, 2013Chen Liu, Xin Qi, Qian Zhao, Jingjuan Yufirst strand synthesis was carried out according to the protocol of the Reverse Transcription System (A3500, Promega). RT-PCR amplification was performed using the same primer pair (Primer 1; Primer 2) used for

(483) Expression of tyrosine hydroxylase and growth-associated protein 43 in aging patients with atrial fibrillation of Xinjiang Uygur and Han nationalitiesAfrican Journal of Pharmacy and PharmacologyMarch 22, 2013Yao-Dong Lireverse transcriptased into cDNA according to the instruction of the Reverse Transcription Kit (Promega Company A3500). Design and synthesis of the primer: primer was synthesized by TaKaRa Company (Table 1). Real-time PCR system

(484) Global transcriptional profiling of the postmortem brain of a patient with G114V genetic Creutzfeldt-Jakob diseaseInternational Journal of Molecular MedicineMarch 1, 2013CHAN TIAN, DI LIU, CHEN CHEN, YIN XU, HAN-SHI GONG, CAO CHEN, QI SHI, BAO-YUN ZHANG, JUN HAN, XIAO-PING DONGcDNA synthesis, 2 µg of treated RNAs were mixed with the reagents in Reverse Transcription System (cat. #A3500; Promega). The real-time PCR was carried out on an ABI Prism 7900 sequencing detector, at the conditions

(485) Measles virus nucleocapsid protein, a key contributor to Paget's disease, increases IL-6 expression via down-regulation of FoxO3/Sirt1 signalingBoneMarch 1, 2013Feng-Ming Wang, Aliye Sarmasik, Yuko Hiruma, Quanhong Sun, Bénédicte Sammut, Jolene J. Windle, G. David Roodman, Deborah L. Galsoninstructions. Reverse transcription was performed using a reverse transcription system kit (Promega, Cat.A3500). An aliquot of the product cDNA was used for real-time PCR with iQ™ SYBR Green Supermix and iCycler

(486) An Intron Mutation in the ACVRL1 May Be Associated with a Transcriptional Regulation Defect in a Chinese Family with Hereditary Hemorrhagic TelangiectasiaPLoS ONEFebruary 27, 2013Qian Yu, Xiao-hui Shen, Ying Li, Rui-juan Li, Ji Li, Yun-ya Luo, Su-fang Liu, Ming-yang Deng, Min-fei Pei, Guang-sen Zhangcellular RNA was extracted using Trizol reagents (Invitrogen). The RNA was reverse-transcribed using A3500 Reverse Transcription System (Promega). Primer sequences were as follows: human ACVRL1-specific primers,

(487) STAT2 deficiency and susceptibility to viral illness in humansProceedings of the National Academy of Sciences of the United States of AmericaFebruary 19, 2013Hambleton S, Goodbourn S, Young DF, Dickinson P, Mohamad SM, Valappil M, McGovern N, Cant AJ, Hackett SJ, Ghazal P, et al.by using random primers and AMV reverse transcriptase by using Promega reverse transcription system A3500 according to the manufacturer’s instructions. RT-PCR of exons 3 to 6 was performed by using the following

(488) A Clinical Evaluation of Statin Pleiotropy: Statins Selectively and Dose-Dependently Reduce Vascular InflammationPLoS ONEJanuary 22, 2013Evelien van der Meij et al.Scientific, Veenendaal, The Netherlands) and glass beads [10]. Complementary-DNA was prepared using kit #A3500 (Promega, Leiden, The Netherlands) and quantitative real-time polymerase chain reaction (QRT-PCR) analysis

(489) Increased learning and brain long-term potentiation in aged mice lacking DNA polymerase μPloS oneJanuary 1, 2013Lucas D, Delgado-García JM, Escudero B, Albo C, Aza A, Acín-Pérez R, Torres Y, Moreno P, Enríquez JA, Samper E, et al.by the manufacturer. cDNA synthesis was performed using a Reverse Transcription kit (Promega, P/N: A3500) following the manufacturer’s protocol. Gene expression was evaluated by Real Time PCR or TaqMan assay

(490) Ethanol facilitates hepatitis C virus replication via up-regulation of GW182 and heat shock protein 90 in human hepatoma cellsHepatology (Baltimore, Md.)January 1, 2013Bukong TN, Hou W, Kodys K, Szabo G.viral RNA was then reverse transcribed to cDNA using the Promega Reverse Transcription System (Cat # A3500) in a 20μL final reaction volume. 5μL of viral DNA was then used for real time PCR along with 5uL of

(491) The immunoregulatory effects of CMV-infection in human fibroblasts and the impact on cellular senescenceImmunity & AgeingDecember 1, 2012Juliane Wolf, Birgit Weinberger, Beatrix Grubeck-Loebensteinof total RNA was used for cDNA synthesis, which was performed using the Reverse Transcription system A3500 with Oligo (dT)-primers (Promega, Madison, USA). cDNA sequences were amplified by qRT-PCR using the

(492) Nonprimate Hepaciviruses in Domestic Horses, United KingdomEmerging Infectious DiseasesDecember 1, 2012Sinéad Lyons, Amit Kapoor, Colin Sharp, Bradley S. Schneider, Nathan D. Wolfe, Geoff Culshaw, Brendan Corcoran, Bruce C. McGorum, Peter Simmondsof 100 μL. RNA was converted to cDNA by using random hexamers with the Reverse Transcription System A3500 (Promega) and then used in nested PCR with previously published CHV NS3 primers (22) Chv-0F1,Chv-0R1S1,

(493) Genome-wide gene expression profiling of introgressed indica rice alleles associated with seedling cold tolerance improvement in a japonica rice backgroundBMC GenomicsDecember 1, 2012Fan Zhang, Liyu Huang, Wensheng Wang, Xiuqin Zhao, Linghua Zhu, Binying Fu, Zhikang Liusing 2 μg of total RNA in a 100 μL reaction mixture using a Reverse Transcription System (Promega, A3500). Diluted synthesized cDNA (1 μL) was used for qRT-PCR analysis along with Page 13 of 15 200 nM of

(494) Self-antigen recognition by follicular lymphoma B-cell receptorsBloodNovember 15, 2012Kacey L. Sachen, Michael J. Strohman, Jonathan Singletary, Ash A. Alizadeh, Nicole H. Kattah, Chen Lossos, Elizabeth D. Mellins, Shoshana Levy, Ronald Levywere further expanded. RNA was extracted using RNeasy (QIAGEN), followed by synthesis of cDNA (Promega A3500) and amplification of VH and VL regions using leader family primers to VH2 (5⬘-ATGGACATACTTTGTTCCAC-3⬘)

(495) The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCROncology LettersOctober 1, 2012QIN-YUN MA, LIE-WEN PANG, ZHI-MING CHEN, YONG-JUN ZHU, GANG CHEN, JI CHEN(Promega Corporation, Madison WI, USA) was used for DNA treatment. The Reverse Transcription System A3500 (Promega Corporation) was used for reverse transcription (RT) reactions, and SYBR-Green PCR Master mix

(496) Cytokine-like 1 is involved in the growth and metastasis of neuroblastoma cellsInternational Journal of OncologyOctober 1, 2012MINGJIE WEN, HUANMIN WANG, XULONG ZHANG, JUN LONG, ZHE LV, QINGLI KONG, YUNQING ANin a 20 µl reaction volume using oligo dT(15) primers and the M-MLV reverse transcriptase (Promega A3500, Madison, WI, USA). The levels of the CYTL1 and β-actin (internal control) transcripts were analyzed

(497) Genome-wide gene expression profiling of introgressed indica rice alleles associated with seedling cold tolerance improvement in a japonica rice backgroundBMC GenomicsSeptember 7, 2012Fan Zhang, Liyu Huang, Wensheng Wang, Xiuqin Zhao, Linghua Zhu, Binying Fu, Zhikang Liusing 2 μg of total RNA in a 100 μL reaction mixture using a Reverse Transcription System (Promega, A3500). Diluted synthesized cDNA (1 μL) was used for qRT-PCR analysis along with Page 13 of 15 200 nM of

(498) Secreted Protein Acidic and Rich in Cysteine (SPARC) Suppresses Angiogenesis by Down-Regulating the Expression of VEGF and MMP-7 in Gastric CancerPLoS ONESeptember 5, 2012Jun-Ling Zhang et al.RQ1 DNase (Promega, Madison, WI, USA). RNA was reverse transcribed using AMV Reverse Transcriptase (A3500, Promega, Madison, WI, USA). Quantitative real-time PCR was performed in an ABI Prism7300 Sequence Detection

(499) Carcinoma origin dictates differential skewing of monocyte functionOncoimmunologySeptember 1, 2012Bögels M, Braster R, Nijland PG, Gül N, van de Luijtgaarden W, Fijneman RJ, Meijer GA, Jimenez CR, Beelen RH, van Egmond M.instructions. mRNA was directly used for cDNA synthesis using Promega cDNA synthesis kits (Promega, a3500). For semi-quantitative real time PCR, 2 µl of diluted cDNA was mixed with 4 µl SYBR-green (Applied Biosystems

(500) Induced Pluripotent Stem Cells from Patients with Huntington's Disease Show CAG-Repeat-Expansion-Associated PhenotypesCell Stem CellAugust 3, 2012Virginia B Mattis et al.Promega). Complementary DNA was generated from 0.5–2µg total RNA using a Reverse Transcript System (Promega A3500 or Invitrogen). qRT–PCR and primer sequences are described in supplemental methods. Immunocytochemistry Cells

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