Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(521) Proteomic Approach to Evaluate Mechanisms That Contribute to Food Allergenicity: Comparative 2D-DIGE Analysis of Radioallergosorbent Test Positive and Negative PatientsInternational Journal of ProteomicsSeptember 19, 2011Bindukumar Nair, John C. Wheeler, Donald E. Sykes, Paula Brown, Jessica L. Reynolds, Ravikumar Aalinkeel, Supriya D. Mahajan, Stanley A. Schwartzto cDNA using the reverse transcriptase kit from Promega (Promega Inc, Madison, Wis, USA; Cat number A3500). Relative abundance of each mRNA species is quantitated by real time quantitative PCR for specific

(522) Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafunginJournal of Medical MicrobiologySeptember 1, 2011Soňa Kucharíková, Hélène Tournu, Katrien Lagrou, Patrick Van Dijck, Helena Bujdákovátreated with amplification-grade DNase I and used for cDNA synthesis using a Reverse Transcription kit (A3500; Promega). Quantitative PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems)

(523) Silk peptides inhibit adipocyte differentiation through modulation of the Notch pathway in C3H10T1/2 cellsNutrition research (New York, N.Y.)September 1, 2011Jung SR, Song NJ, Hwang HS, An JJ, Cho YJ, Kweon HY, Kang SW, Lee KG, Yoon K, Kim BJ, et al.

(524) Effect of fasting on digestive gland lipase transcripts expression in Penaeus vannameiMarine GenomicsJuly 8, 2011Crisalejandra Rivera-Pérez, Fernando García-Carreño

(525) Fabp3 inhibits proliferation and promotes apoptosis of embryonic myocardial cellsCell biochemistry and biophysicsJuly 1, 2011Zhu C, Hu DL, Liu YQ, Zhang QJ, Chen FK, Kong XQ, Cao KJ, Zhang JS, Qian LM.CA, USA). cDNA was synthesized from 1 μg of total RNA using an AMV Reverse Transcriptase Kit (Promega A3500; Promega, Madison, WI, USA). An aliquot (10%) of the resulting cDNA was amplified with the primers listed

(526) Interleukin-1ß induces cyclooxygenase-2 expression and promotes the invasive ability of human mesenchymal stem cells derived from ovarian endometriomaFertility and SterilityJune 14, 2011An-Pei Kao M.S., Kai-Hung Wang Ph.D., Cheng-Yu Long M.D. Ph.D., Chee-Yin Chai M.D. Ph.D., Cheng-Fang Tsai B.S., Tsung-Hua Hsieh M.S., Chia-Yi Hsu M.S., Chia-Cheng Chang Ph.D., Jau-Nan Lee M.D. Ph.D., Eing-Mei Tsai M.D. Ph.D.

(527) Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0Virology journalMay 25, 2011Kamakura M, Goshima F, Luo C, Kimura H, Nishiyama Y.from 1 μg total RNA using the Promega reverse transcription system (Promega, Madison, Wisconsin, USA, A3500), and cDNA was used as a PCR template for TaKaRa Ex Taq (TAKARA BIO INC, Shiga, Japan, RR001A). The

(528) DNA hypermethylation of the NOX5 gene in fetal ventricular septal defectExperimental and Therapeutic MedicineMay 11, 2011CHUN ZHU, ZHANG-BIN YU, XIAO-HUI CHEN, CHEN-BO JI, LING-MEI QIAN, SHU-PING HAN(Invitrogen). cDNA was synthesized from 1 µg of total RNA using an AMV Reverse Transcriptase kit (Promega A3500; Promega, Madison, WI, USA). An aliquot (10%) of the resulting cDNA was amplified for PCR with the primers

(529) Astragaloside IV synergizes with ferulic acid to inhibit renal tubulointerstitial fibrosis in rats with obstructive nephropathyBritish Journal of PharmacologyApril 1, 2011L Q Meng, J W Tang, Y Wang, J R Zhao, M Y Shang, M Zhang, S Y Liu, L Qu, S Q Cai, X M Lito the manufacturer's instructions. Template cDNA was prepared using a Reverse Transcription System A3500 (Promega, Madison, WI) and quantified by real-time PCR using the FastStart Universal SYBR Green Master (ROX

(530) Augmented NADPH oxidase activity and p22phox expression in monocytes underlie oxidative stress of patients with type 2 diabetes mellitusDiabetes research and clinical practiceMarch 1, 2011Huang X, Sun M, Li D, Liu J, Guo H, Dong Y, Jiang L, Pan Q, Man Y, Wang S, et al.

(531) PP2A inhibitors induce apoptosis in pancreatic cancer cell line PANC-1 through persistent phosphorylation of IKKa and sustained activation of the NF-?B pathwayCancer LettersFebruary 9, 2011Wei Li, Zheng Chen, Yang Zong, Feiran Gong, Yi Zhu, Yunxia Zhu, Jinghuan Lv, Jingjing Zhang, Li Xie, Yujie Sun, Yi Miao, Min Tao, Xiao Han, Zekuan Xu

(532) Mutation in the TCRα subunit constant gene (TRAC) leads to a human immunodeficiency disorder characterized by a lack of TCRαβ+ T cellsThe Journal of clinical investigationFebruary 1, 2011Morgan NV, Goddard S, Cardno TS, McDonald D, Rahman F, Barge D, Ciupek A, Straatman-Iwanowska A, Pasha S, Guckian M, et al.AMV reverse transcriptase using the Promega reverse transcription system according to the manufacturer’s instructions (A3500, Promega). RT-PCR of TRAC was performed using the following primers: TRAC2F (5′-GTTCCTGTGATGTCAAGCTGGTC-3′)

(533) Regulation of unfolded protein response modulator XBP1s by acetylation and deacetylationBiochem. J.January 1, 2011Feng-Ming Wang, Hong-Jiao Ouyanginstructions. Reverse transcription was conducted using a reverse transcription system kit (Promega, Cat.A3500). The aliquot of the product cDNA was used for real-time PCR with iQ™ SYBR Green Supermix and iCycler

(534) Antibody responses to NY-ESO-1 in primary breast cancer identify a subtype target for immunotherapyPloS oneJanuary 1, 2011Hamaï A, Duperrier-Amouriaux K, Pignon P, Raimbaud I, Memeo L, Colarossi C, Canzonieri V, Perin T, Classe JM, Campone M, et al.72 h following transfection. cDNA synthesis was performed using Promega Reverse Transcription System A3500 (Promega). Semi-quantitative PCR was performed using GoTaq® Flexi DNA Polymerase (Promega). cDNA integrity

(535) The chemotherapeutic drug 5-fluorouracil promotes PKR-mediated apoptosis in a p53-independent manner in colon and breast cancer cellsPloS oneJanuary 1, 2011García MA, Carrasco E, Aguilera M, Alvarez P, Rivas C, Campos JM, Prados JC, Calleja MA, Esteban M, Marchal JA, et al.74106), and sequentially complementary DNA was generated using reverse transcription system (Promega A3500), both strictly following the manufacturing instructions. Real-time-PCR Quantitative was performed in

(536) Regulation of unfolded protein response modulator XBP1s by acetylation and deacetylationBiochemical JournalJanuary 1, 2011Feng-Ming Wang, Yi-Jiun Chen, Hong-Jiao Ouyanginstructions. Reverse transcription was conducted using a reverse transcription system kit (Promega, Cat.A3500). The aliquot of the product cDNA was used for real-time PCR with iQ™ SYBR Green Supermix and iCycler

(537) Expressed sequence identification and characterization of the cDNA for Interleukin-4 from the mitogen-stimulated lymphoid tissue of a marsupial, Macropus eugeniiVeterinary Immunology and ImmunopathologyDecember 8, 2010Lauren Young

(538) Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytesInternational journal of molecular medicineDecember 1, 2010Qin DN, Kou CZ, Ni YH, Zhang CM, Zhu JG, Zhu C, Wang YP, Zhu GZ, Shi C, Ji CB, et al.260 nm. cDNA was synthesized from 1 μg of total RNA using an AMV Reverse Transcriptase Kit (Promega A3500; Madison, WI, USA). qPCR (SYBR Green method) was performed in an Applied Biosystems 7500 Sequence Detection

(539) Increasing CD44+/CD24(-) tumor stem cells, and upregulation of COX-2 and HDAC6, as major functions of HER2 in breast tumorigenesisMolecular CancerDecember 1, 2010Kai-Hung Wang, An-Pei Kao, Chia-Cheng Chang, Jau-Nan Lee, Ming-Feng Hou, Cheng-Yu Long, Hung-Sheng Chen, Eing-Mei Tsaicells were used to synthesize the first-strand cDNA, using the Reverse Transcription System (Promega, A3500) according to the manufacturer's protocol. PCR amplification was carried out by using 1 μL of the first-strand

(540) Novel TSHR mutations in consanguineous families with congenital nongoitrous hypothyroidismClinical EndocrinologyNovember 1, 2010Hakan Cangul et al.synthesized using random primers and AMV reverse transcriptase using the Promega reverse transcription system A3500 according to manufacturers’ instructions (Promega). The primers used for RT-PCR and sequencing reactions

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