Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(541) Reduced Immunoproteasome Formation and Accumulation of Immunoproteasomal Precursors in the Brains of Lymphocytic Choriomeningitis Virus-Infected MiceThe Journal of ImmunologyNovember 1, 2010Marcel Kremer, Anja Henn, Cornelia Kolb, Michael Basler, Jacqueline Moebius, Benoît Guillaume, Marcel Leist, Benoît J. Van den Eynde, Marcus Groettrupsynthesis of single-stranded cDNA from pure total RNA, the ReverseTranscription System Kit (product No.A3500; Promega, Mannheim, Germany) was used. To determine relative gene expression, the LightCycler Fast Start DNA

(542) LYRM1, a Gene that Promotes Proliferation and Inhibits Apoptosis during Heart DevelopmentMoleculesOctober 11, 2010Chun Zhu, Yao-Qiu Liu, Fu-Kun Chen, De-Liang Hu, Zhang-Bin Yu, Ling-Mei QianUSA). Then, cDNA was synthesized from 1 μg of total RNA using an AMV Reverse Transcriptase Kit (Promega A3500; Promega, Madison, WI, USA). Real-time PCR was performed using the SYBR Green method in an Applied Biosystems

(543) Andrographolide attenuates tumor necrosis factor-alpha-induced insulin resistance in 3T3-L1 adipocytesMolecular and Cellular EndocrinologyOctober 5, 2010Lina Jin, Guojun Shi, Guang Ning, Xiaoying Li, Zhiguo Zhang

(544) Ubiquitin D is correlated with colon cancer progression and predicts recurrence for stage II-III disease after curative surgeryBritish Journal of CancerSeptember 28, 2010D-W Yan et al.(Qiagen, Hilden, Germany), and then 1 μg RNA was reverse transcribed into complementary DNA using an A3500 RT-PCR System (Promega Corporation, Madison, WI, USA). The primers used for RT-PCR were: UBD, sense

(545) Effects of Korean white ginseng extracts on obesity in high-fat diet-induced obese miceCytotechnologyAugust 1, 2010Young-Sil Lee, Byung-Yoon Cha, Kohji Yamaguchi, Sun-Sil Choi, Takayuki Yonezawa, Toshiaki Teruya, Kazuo Nagai, Je-Tae Woosample were reverse-transcribed to cDNA according to the protocol of the reverse transcription system (a3500, Promega, USA). Quantification of gene transcripts for SREBP-1c, FAS, LPL and DGAT1 was completed using

(546) Analysis of X chromosome genomic DNA sequence copy number variation associated with premature ovarian failure (POF)Human ReproductionAugust 1, 2010C. R. Quilter, A. C. Karcanias, M. R. Bagga, S. Duncan, A. Murray, G. S. Conway, C. A. Sargent, N. A. Affara(Austin, TX, USA) and from each RNA sample, cDNA was prepared using an AMV Reverse Transciption Kit (A3500: Promega, Madison, WI, USA) according to the manufacturer’s instructions. Primer pairs were designed

(547) Epidemiology and Clinical Presentations of the Four Human Coronaviruses 229E, HKU1, NL63, and OC43 Detected over 3 Years Using a Novel Multiplex Real-Time PCR MethodJournal of Clinical MicrobiologyAugust 1, 2010E. R. Gaunt, A. Hardie, E. C. J. Claas, P. Simmonds, K. E. Templetonso many samples. Reverse transcription was conducted using a Promega (Southampton, United Kingdom) A3500 reverse transcription system. Each 20-␮l reaction mixture included 4 ␮l of MgCl2 (25 mM), 2 ␮l of 2⫻

(548) Development of a Bacillus subtilis expression system using the improved Pglv promoterMicrobial Cell FactoriesJuly 10, 2010Yang M Ming, Zhang W Wei, Chen Y Lin, Gong Y Shengkit (Cat. Z3100, Promega). The cDNA chain was synthesized by using Reverse Transcription System (Cat. A3500, Promega). And Real-time PCR was performed by using Real time PCR Kit (Cat. DRR041 S, TaKaRa). The bagB

(549) TNF-a induces mitochondrial dysfunction in 3T3-L1 adipocytesMolecular and Cellular EndocrinologyJuly 8, 2010Xiao-Hui Chen, Ya-Ping Zhao, Mei Xue, Chen-Bo Ji, Chun-Lin Gao, Jin-Gai Zhu, Da-Ni Qin, Chun-Zhao Kou, Xiao-Hong Qin, Mei-Ling Tong, Xi-Rong Guo

(550) Effects of liquiritigenin treatment on the learning and memory deficits induced by amyloid beta-peptide (25-35) in ratsBehavioural brain researchJune 26, 2010Liu RT, Zou LB, Fu JY, Lu QJ.

(551) Promotion of rat brain-derived progenitor cell neurogenesis by liquiritigenin treatment: Underlying mechanismsNeuroscience LettersJune 22, 2010Rui Ting Liu, Li Bo Zou, Jie Ying Fu, Qiu Jun Lu

(552) The pathophysiology of abdominal aortic aneurysm growth: corresponding and discordant inflammatory and proteolytic processes in abdominal aortic and popliteal artery aneurysmsJournal of vascular surgeryJune 1, 2010Abdul-Hussien H, Hanemaaijer R, Kleemann R, Verhaaren BF, van Bockel JH, Lindeman JH.

(553) Gene transcript accumulation, tissue and subcellular localization of anthocyanidin synthase (ANS) in developing grape berriesPlant ScienceApril 6, 2010Huiling Wang, Wei Wang, Ping Zhang, QiuHong Pan, Jicheng Zhan, Weidong Huang

(554) Transdifferentiation of fibroblasts into adipocyte-like cells by chicken adipogenic transcription factorsComparative Biochemistry and Physiology, Part AApril 6, 2010Shuang Liu, Yuxiang Wang, Li Wang, Ning Wang, Yumao Li, Hui Li

(555) NOX3-derived reactive oxygen species promote TNF-alpha-induced reductions in hepatocyte glycogen levels via a JNK pathwayFEBS lettersMarch 5, 2010Li L, He Q, Huang X, Man Y, Zhou Y, Wang S, Wang J, Li J.

(556) Mutations in SLC29A3, Encoding an Equilibrative Nucleoside Transporter ENT3, Cause a Familial Histiocytosis Syndrome (Faisalabad Histiocytosis) and Familial Rosai-Dorfman DiseasePLoS GeneticsFebruary 5, 2010Neil V. Morgan et al.synthesized using random primers and AMV reverse transcriptase using the Promega reverse transcription system A3500 according to manufacturers' instructions (Promega). RT-PCR was performed using the following primers:

(557) STEAP4, a gene associated with insulin sensitivity, is regulated by several adipokines in human adipocytesInternational Journal of Molecular MedicineFebruary 4, 2010Xiaohui Chen, Chun Zhu, Chenbo Ji, Yaping Zhao, Chunmei Zhang, Fukun Chen, Chunlin Gao, Jingai Zhu, Lingmei Qian, Xirong Guo260 nm. cDNA was synthesized from 1 μg of total RNA using an AMV Reverse Transcriptase Kit (Promega A3500; Promega, Madison, WI, USA). Real-time RT-PCR (TaqMan fluorescence method or SYBR Green method) was

(558) Nobiletin improves hyperglycemia and insulin resistance in obese diabetic ob/ob miceBiochemical PharmacologyJanuary 29, 2010Young-Sil Lee, Byung-Yoon Cha, Kiyoto Saito, Hiroshi Yamakawa, Sun-Sil Choi, Kohji Yamaguchi, Takayuki Yonezawa, Toshiaki Teruya, Kazuo Nagai, Je-Tae Woo

(559) Identification of Differential Gene Expression in Brassica rapa Nectaries through Expressed Sequence Tag AnalysisPLoS ONEJanuary 20, 2010Marshall Hampton, Wayne W. Xu, Brian W. Kram, Emily M. Chambers, Jerad S. Ehrnriter, Jonathan H. Gralewski, Teresa Joyal, Clay J. Carterpresence of transcripts with Promega Corporation's (Madison, WI, USA) Reverse Transcription System (A3500), in conjunction with GoTaq Green Master Mix (Promega, M7122), according to the manufacturer's instructions.

(560) CELL WALL INVERTASE 4 is required for nectar production in ArabidopsisJournal of Experimental BotanyJanuary 1, 2010Jeffrey M. Ruhlmann, Brian W. Kram, Clay J. Carterwas used to examine the presence of CWINV4 transcripts with the Promega Reverse Transcription System (A3500) according to the manufacturer's instructions with 0.1 μg of input RNA. Partial cDNA sequences for BrCWINV4

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