Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(421) Deficient plastidic fatty acid synthesis triggers cell death by modulating mitochondrial reactive oxygen speciesCell ResearchMay 1, 2015Jian Wu et al.Invitrogen) and 12.5 μg of total RNA was treated with DNase I and used for cDNA synthesis with RT kit (Cat# A3500, Promega). RT-qPCR experiments were performed using the Bio-Rad CFX96 System. The primer sequences used

(422) Aberrant methylation of the SPARC gene promoter and its clinical implication in gastric cancerScientific ReportsMay 1, 2015Zi-Yi Chen et al.following the manufacturer's instructions. RNA was reverse transcribed using AMV reverse transcriptase (A3500, Promega, Madison, WI, USA), and aliquots of the reaction mixture were used for subsequent PCR amplification. Primers

(423) Maternal protein restriction induces early‐onset glucose intolerance and alters hepatic genes expression in the peroxisome proliferator‐activated receptor pathway in offspringJournal of Diabetes InvestigationMay 1, 2015Jia Zheng, Xinhua Xiao, Qian Zhang, Miao Yu, Jianping Xu, Zhixin WangRNA was reverse transcribed by 1 μg of total RNA from each sample using the Power cDNA Synthesis kit (A3500; Promega BioSciences LLC, Sunnyvale, CA, USA). The cDNA (2 μL) was amplified with the SYBR® Green PCR

(424) Global DNA methylation was changed by a maternal high-lipid, high-energy diet during gestation and lactation in male adult mice liverBritish Journal of NutritionApril 14, 2015Huan-Ling Yu, Shan Dong, Li-Fang Gao, Li Li, Yuan-Di Xi, Wei-Wei Ma, Lin-Hong Yuan, Rong Xiaoreal-time PCR The total RNA that had been purified earlier was reverse transcribed using an RT kit (no. A3500; Applied Promega). The mRNA encoding PPARg, liver X receptor a (LXRa), cluster of differentiation 36

(425) MicroRNA-182 inhibits proliferation through targeting oncogenic ANUBL1 in gastric cancerOncology ReportsApril 1, 2015LIN TANG, FENG CHEN, ER-JUN PANG, ZHI-QI ZHANG, BING-WEI JIN, WEI-FENG DONG(RT) system followed by PCR amplification in a 50 µl PCR system performed using an RT-PCR kit (cat no. A3500, Promega, Madison, WI, USA). Housekeeping gene Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was

(426) Decreased expression of SCUBE2 is associated with progression and prognosis in colorectal cancerOncology reportsApril 1, 2015Song Q, Li C, Feng X, Yu A, Tang H, Peng Z, Wang X.instructions (Qiagen, Hilden, Germany). First-strand cDNA was synthesized from 1 µg of RNA using the A3500 RT-PCR System (Promega, Madison, WI, USA). The primers used for quantitative PCR were: SCUBE2, forward:

(427) Genistein Alleviates β-Amyloid-Induced Inflammatory Damage Through Regulating Toll-Like Receptor 4/Nuclear Factor κBJournal of Medicinal FoodMarch 1, 2015Weiwei Ma, Bingjie Ding, Huanling Yu, Linhong Yuan, Yuandi Xi, Rong Xiaoimmunoassay using a com- Total RNA was purified using TRIzol (Invitrogen). A reverse transcriptase kit (A3500; Applied Promega) was used for reverse transcription. The mRNA expression of TLR4, IjB-a, and b-actin

(428) Depletion of the IKBKAP ortholog in zebrafish leads to hirschsprung disease-like phenotypeWorld journal of gastroenterologyFebruary 21, 2015Cheng WW, Tang CS, Gui HS, So MT, Lui VC, Tam PK, Garcia-Barcelo MM.manufacturer’s proto col. 1 μg of RNA was used for cDNA synthesis by the Reverse Transcription System (Promega A3500) using oligo-dT primer. For qualitative realtime-polymerase chain reaction (RT-PCR), standard ® PCR

(429) Augmented Rac1 Expression and Activity are Associated with Oxidative Stress and Decline of β Cell Function in ObesityCellular Physiology and BiochemistryFebruary 9, 2015Shutong Zhou, Dongni Yu, Shangyong Ning, Heli Zhang, Lei Jiang, Lei He, Miao Li, Mingxiao SunTrizol reagent following the manufacturers instructions. Reverse transcription of RNA was performed with A3500 kit (Promega) according to the manufacturer’s instructions. Immunoﬂuorescence in vitro 1×105 NIT-1β

(430) Invasive Salmonella Typhimurium ST313 with Naturally Attenuated Flagellin Elicits Reduced Inflammation and Replicates within MacrophagesPLoS Neglected Tropical DiseasesJanuary 8, 2015Girish Ramachandran, Darren J. Perkins, Patrick J. Schmidlein, Mohan E. Tulapurkar, Sharon M. Tennantmanufacturer's instructions. A total of 1 µg of RNA was utilized in oligo(dT) cDNA synthesis (Promega RT system A3500). qRT-PCR was carried out using an ABI Prism 7900HT Sequence Detection System (Applied Biosystems) utilizing

(431) Foxp1 Regulates the Proliferation of Hair Follicle Stem Cells in Response to Oxidative Stress during Hair CyclingPloS oneJanuary 1, 2015Zhao J, Li H, Zhou R, Ma G, Dekker JD, Tucker HO, Yao Z, Guo X.(Invitrogen, 15596–026, USA). cDNA was then synthesized using the Reverse Transcription System (Promega, A3500, USA). The quantitative RT-PCRs were performed on an Applied Biosystems 7500 using SYBR reagents (Roche,

(432) Whole Exome Sequencing Identified MCM2 as a Novel Causative Gene for Autosomal Dominant Nonsyndromic Deafness in a Chinese FamilyPloS oneJanuary 1, 2015Gao J, Wang Q, Dong C, Chen S, Qi Y, Liu Y.cochleae with Trizol (Life Technologies) and reverse-transcribed using the Reverse Transcript system (A3500, Promega); PCR analysis was carried out using a GoTaq Hot Start Green Master Mix (M5122, Promega). Specific

(433) Normocaloric Low Cholesterol Diet Modulates Th17/Treg Balance in Patients with Chronic Hepatitis C Virus InfectionPLoS ONEDecember 22, 2014Roberta Maggio, Carmela Viscomi, Paola Andreozzi, Gabriella D'Ettorre, Giovanni Viscogliosi, Barbara Barbaro, Manuele Gori, Vincenzo Vullo, Clara Balsanowas prepared using Random Primers, and AMV Reverse Transcriptase of the Reverse Transcription System (A3500, Promega) according to the manufacturer's protocol. PCR primers were designed using AmplifX 1.5.4 software,

(434) Effects of a novel curcumin derivative on insulin synthesis and secretion in streptozotocin-treated rat pancreatic islets in vitroChinese MedicineDecember 1, 2014Mohammed Talaat Abdel Aziz et al.transcription The extracted RNA was reverse-transcribed into cDNA by a Reverse Transcription System Kit (Cat. no. A3500; Promega, Madison, WI, USA). The cDNA was generated from 5 μg of total RNA extracted with 1 μL (20 pmol)

(435) Identification of genes directly regulated by a transcription factor in riceProtocol ExchangeNovember 25, 2014Andy Pereira, Supratim Basu, Arjun Krishnan, Madana Ambavaram, Lutfor Rahman, Venkategowda Ramegowda, Andy PereiraChemiluminescent EMSA kit **Luciferase analysis** Trizol \(Invitrogen) Reverse Trasncription System \(Promega, A3500) qPCR master mix \ (A6001) Dual-Glo® Luciferase Assay System \( Promega, E2940) **Plasmids Used** pGL3-Basic

(436) Ascorbic Acid Inhibition of Candida albicans Hsp90-Mediated Morphogenesis Occurs via the Transcriptional Regulator Upc2Eukaryotic CellOctober 1, 2014Frédérique Van Hauwenhuyse, Alessandro Fiori, Patrick Van Dijckor GdA (10 ␮M) before RNA extraction. RNA was DNase treated prior to cDNA synthesis with the Promega A3500 reverse transcription kit. Quantitative real-time PCR was performed using the GoScript Reverse Transcription System

(437) Maternal High-Fat Diet Modulates Hepatic Glucose, Lipid Homeostasis and Gene Expression in the PPAR Pathway in the Early Life of OffspringInternational Journal of Molecular SciencesAugust 25, 2014Jia Zheng, Xinhua Xiao, Qian Zhang, Miao Yu, Jianping Xu, Zhixin WangRNA was reverse transcribed by 1 μg of total RNA from each sample using the Power cDNA Synthesis kit (A3500, Promega BioSciences LLC, Sunnyvale, CA, USA). The cDNA (2 μL) was amplified with a SYBR® Green PCR

(438) Effect of Dietary Vitamin A on Reproductive Performance and Immune Response of Broiler BreedersPLoS ONEAugust 22, 2014Jianmin Yuan, Abdelfatah Rashad Roshdy, Yuming Guo, Yongwei Wang, Shuangshuang GuoUSA) using an oligo (dT) 15 primer in the presence of recombinant RNase in Ribonuclease Inhibitor (A3500; Promega, Madison, WI, USA). Real-time polymerase chain reaction (PCR) analysis of vitamin D (1,25-dihydroxyvitamin

(439) TLR9 Expression in Uterine Cervical Lesions of Uyghur Women Correlate with Cervical Cancer Progression and Selective Silencing of Human Papillomavirus 16 E6 and E7 Oncoproteins in VitroAsian Pacific Journal of Cancer PreventionAugust 2, 2014Yi Hao, Jian-Ling Yuan, Abulizi Abudula, Axiangu Hasimu, Nafeisha Kadeer, Xia GuocDNA Synthesis, cDNA was synthesized with First-Strand Synthesis System for RT-PCR Kit (Catalog No.A3500 Promega). RT-PCR analyses were performed using the following primer sets, human TLR9 (sense: 5’-CCGTGACAATTACCTGGCCTTC-3’, antisense:

(440) Inhibitor of DNA-binding protein 1 knockdown arrests the growth of colorectal cancer cells and suppresses hepatic metastasis in vivoOncology ReportsJuly 1, 2014XIAOLAN LAI, JINRONG LIAO, WANSONG LIN, CHUANZHONG HUANG, JIEYU LI, JIZHEN LIN, QIANG CHEN, YUNBIN YE(Invitrogen). One microgram of total RNA was reverse-transcribed using the Promega Reverse Transcription System A3500 (Promega). Quantitative real-time polymerase chain reaction (qRT-PCR) was run on a LightCycler Roche

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