Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(461) Identification of proteins related to epigenetic regulation in the malignant transformation of aberrant karyotypic human embryonic stem cells by quantitative proteomicsPloS oneJanuary 1, 2014Sun Y, Yang Y, Zeng S, Tan Y, Lu G, Lin G.instructions. Two microgram of RNA per sample was reverse-transcribed into first-strand cDNA by using the A3500 reverse transcription system (Promega, USA) in a standard protocol with random oligo (dT) primers. According

(462) Identification of novel modifiers of Aβ toxicity by transcriptomic analysis in the fruitflyScientific ReportsDecember 16, 2013G. Favrin, D. M. Bean, E. Bilsland, H. Boyer, B. E. Fischer, S. Russell, D. C. Crowther, H. A. Baylis, S. G. Oliver, M. E. GiannakouScientific, UK) and cDNA was prepared using oligo-d(T) primers and a Promega Reverse transcription kit (#A3500) according to the manufacturer's protocol (Promega, Southampton, UK). qRT-PCR was performed using a

(463) The effect of TrkA signaling pathway on the expressions of TRPC1，TRPC3 in 6-hydroxydopamine lesioned rat treated by electroacupuncture国际心脑血管病杂志December 16, 2013Yong-peng YU, An-mu XIE, Xiang-lin CHI, Et Al全自动图像分析系统，分别计数 TRPC1、RPC3 阳性细胞数。 1.5 RT-PCR 检测取 50 ng 待测脑组织。按照 Trizol RNA 提取试剂盒提取总 RNA，测定 RNA 浓度。采用 Promega A3500 逆转录试剂盒，按操作说明进行逆转录合成 cDNA，以逆转录所得的 cDNA 为模板进行 PCR 扩增。从 GeneBank 中检索大鼠脑 TRPC1、TRPC3 基因全长序列，按引物设计的基本原则设计引物。TRPC1 ，，，，，上游:

(464) Identification of Biomarkers for Esophageal Squamous Cell Carcinoma Using Feature Selection and Decision Tree MethodsThe Scientific World JournalDecember 12, 2013Chun-Wei Tung, Ming-Tsang Wu, Yu-Kuei Chen, Chun-Chieh Wu, Wei-Chung Chen, Hsien-Pin Li, Shah-Hwa Chou, Deng-Chyang Wu, I-Chen WuTranscription. After RNA isolation, cDNA was prepared from each sample by Reverse Transcription System (Cat: A3500, Promega Corporation, USA). For the appropriate efficiency of reverse transcription, 1 𝜇g total RNA

(465) Signaling mechanisms of a water soluble curcumin derivative in experimental type 1 diabetes with cardiomyopathyDiabetology & Metabolic SyndromeDecember 1, 2013Mohamed Talaat Abdel Aziz, Ibrahim Naguib El Ibrashy, Dimitri P Mikhailidis, Ameen Mahmoud Rezq, Mohamed Abdel Aziz Wassef, Hanan Hassan Fouad, Hanan Hosni Ahmed, Dina A Sabry, Heba Mohamed Shawky, Rania Elsayed HusseinThe extracted RNA was reversed transcribed into cDNA using Reverse Transcription System kit (Cat. # A3500) supplied by Promega (Promega, Madison, WI, USA). cDNA was generated from 5 μg of total RNA extracted

(466) Effect of dietary nonphytate phosphorus on laying performance and small intestinal epithelial phosphate transporter expression in Dwarf pink-shell laying hensJournal of Animal Science and BiotechnologyDecember 1, 2013Wei Nie, Ying Yang, Jianmin Yuan, Zhong Wang, Yuming GuoReverse Transcriptase kit (Promega) in the presence of recombinant RNase in Ribonuclease Inhibitor (A3500, Promega). In total, 1 μg RNA was used and Oligo (dT) 15 was used as the primer. Gene expression of

(467) The persistence of biofilm-associated antibiotic resistance of Staphylococcus aureus isolated from clinical bovine mastitis cases in AustraliaFolia Microbiol (Praha)November 1, 2013Charlene Babra et al.Biotecnologia®). 4.6.2. Síntese do cDNA A síntese do cDNA foi realizada utilizando o kit AMV Reverse Transcriptase A3500 (Promega®) a partir de 1 μg de RNA total, acrescido de 1 mM de dNTP , 5 mM de MgCl2, 0,5 μg de Random

(468) Human Memory Helios⁻ FOXP3⁺ Regulatory T Cells (Tregs) Encompass Induced Tregs That Express Aiolos and Respond to IL-1β by Downregulating Their Suppressor FunctionsThe Journal of ImmunologyNovember 1, 2013Caroline Raffin, Pascale Pignon, Clotilde Celse, Emilie Debien, Danila Valmori, Maha Ayyoubusing the RNeasy Mini Kit (Qiagen) and retrotranscribed using Promega Reverse Transcription System A3500 (Promega). Quantitative PCR (qPCR) was performed with a TaqMan assay on an ABI 7000 system (Applied

(469) Expression of tyrosine hydroxylase and growth-associated protein 43 in aging atrial fibrillation patients of Xinjiang Uygur and Han nationalityGenetics and molecular research : GMROctober 30, 2013Li YD, Li JX, Tang BP, Gan TY, Xu GJ, Zhou XH, Li H, Guo X, Mahemuti A, Sun Q, et al.reverse transcribed into cDNA according to instructions of the Reverse Transcription Kit (Promega Company A3500). The design and synthesis of the primers were performed by the TaKaRa Company (Table 1). The real-time PCR system

(470) Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesisNature CommunicationsOctober 1, 2013Haiyun Gan et al.the Oligo(dT) primer by following the instructions of the Promega Reverse Transcription System (Cat. A3500). The total volume was diluted by four times after the reaction and 1 ml of the solution was used in

(471) Correction: Analysis of Unannotated Equine Transcripts Identified by mRNA SequencingPLoS ONESeptember 19, 2013Stephen J. Coleman, Zheng Zeng, Matthew S. Hestand, Jinze Liu, James N. MacleodPromega Reverse Transcription System reagents and protocol (Promega Corporation, Madison, WI, Cat# A3500) and an equal ratio of oligo-dT and random hexamer primers. PCR was performed on an Eppendorf Mastercycler

(472) Silencing of FABP3 leads to apoptosis-induced mitochondrial dysfunction and stimulates Wnt signaling in zebrafishMolecular Medicine ReportsSeptember 1, 2013YAO-QIU LIU, GUI-XIAN SONG, HAI-LANG LIU, XUE-JIE WANG, YA-HUI SHEN, LI-JUAN ZHOU, JIN JIN, MING LIU, CHUN-MEI SHI, LING-MEI QIANScientific). Complimentary DNA was synthesized from 2 µg total RNA by using an AMV Reverse Transcriptase kit (Promega A3500; Promega, Madison, WI, USA) according to the manufacturer's instructions. qPCR was performed using SYBR‑Green PCR Master mix

(473) β3-Adrenoceptor activation attenuates atherosclerotic plaque formation in ApoE(-/-) mice through lowering blood lipids and glucoseActa Pharmacologica SinicaSeptember 1, 2013Zhao-hong Wang, Yan-fang Li, Yan-qing Guofrom Invitrogen (Carlsbad, CA, USA). The reverse transcriptionpolymerase chain reaction (RT-PCR) kit (A3500) was obtained from Promega (Madison, WI, USA). All primers were synthesized by Invitrogen (Shanghai,

(474) Maternal Dietary Creatine Supplementation Does Not Alter the Capacity for Creatine Synthesis in the Newborn Spiny MouseReproductive SciencesSeptember 1, 2013Hayley Dickinson, Zoe J. Ireland, Domenic A. LaRosa, Bree A. O’Connell, Stacey Ellery, Rod Snow, David W. Walkertranscribed to form complementary DNA according to manufacturer’s instructions (Reverse Transcription System #A3500, Promega) as previously described,5 with the minor modification that placental samples were separated

(475) Aldosterone directly affects apelin expression and secretion in adipocytesJournal of Molecular EndocrinologyAugust 1, 2013He Jiang, Xiao-Ping Ye, Zhong-Yin Yang, Ming Zhan, Hai-Ning Wang, Huang-Min Cao, Hui-Jun Xie, Chun-Ming Pan, Huai-Dong Song, Shuang-Xia Zhaomanufacturer’s instructions. First-strand cDNA was synthesized from total RNA using the RT System (A3500, Promega). Gene expression was analyzed using quantitative RT-PCR with the 2KDDCT relative quantitative method

(476) Analysis of Unannotated Equine Transcripts Identified by mRNA SequencingPLoS ONEJuly 29, 2013Stephen J. Coleman, Zheng Zeng, Matthew S. Hestand, Jinze Liu, James N. MacleodPromega Reverse Transcription System reagents and protocol (Promega Corporation, Madison, WI, Cat# A3500) and an equal ratio of oligo-dT and random hexamer primers. PCR was performed on an Eppendorf Mastercycler

(477) Reprogramming of Murine Macrophages through TLR2 Confers Viral Resistance via TRAF3-Mediated, Enhanced Interferon ProductionPLoS PathogensJuly 11, 2013Darren J. Perkins, Swamy K. Polumuri, Meghan E. Pennini, Wendy Lai, Ping Xie, Stefanie N. Vogelmanufacturer's instructions. A total of 1 µg of RNA was utilized in oligo(dT) cDNA synthesis (Promega RT system A3500). qRT-PCR was carried out using an ABI Prism 7900HT Sequence Detection System (Applied Biosystems) utilizing

(478) Knockdown of FABP3 Impairs Cardiac Development in Zebrafish through the Retinoic Acid Signaling PathwayInternational Journal of Molecular SciencesJuly 3, 2013Xuejie Wang, Lijuan Zhou, Jin Jin, Yang Yang, Guixian Song, Yahui Shen, Hailang Liu, Ming Liu, Chunmei Shi, Lingmei QianComplimentary DNA was synthesized from 2 μg of total RNA by using an AMV Reverse Transcriptase Kit (Promega A3500; Promega, Madison, WI, USA) according to the manufacturer’s instructions. Real-time qPCR was performed

(479) The hybrid Four-CBS-Domain KINβγ subunit functions as the canonical γ subunit of the plant energy sensor SnRK1The Plant JournalJuly 1, 2013Matthew Ramon, Philip Ruelens, Yi Li, Jen Sheen, Koen Geuten, Filip Rollandof total RNA was used for reverse transcription (RT) with the Reverse Transcription System (Promega A3500, Madison, Wi, USA). qPCR was performed using the GoTaq® qPCR Master Mix kit (promega A6001) according

(480) Overexpression of C10orf116 promotes proliferation, inhibits apoptosis and enhances glucose transport in 3T3-L1 adipocytesMolecular Medicine ReportsMay 1, 2013LING CHEN, XIAO-GUANG ZHOU, XIAO-YU ZHOU, CHUN ZHU, CHEN-BO JI, CHUN-MEI SHI, JIE QIU, XI-RONG GUOnm. cDNA was synthesized from 1 µg of total RNA by using an AMV Reverse Transcriptase kit (Promega A3500; Promega Corp., Madison, WI, USA) according to the manufacturer's instructions. Real-time RT-PCR was

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