Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(381) Proliferative effects of gamma-amino butyric acid on oral squamous cell carcinoma cells are associated with mitogen-activated protein kinase signaling pathwaysInternational Journal of Molecular MedicineJuly 1, 2016JING MA, YAN ZHANG, JUN WANG, TIANYU ZHAO, PING JI, JINLIN SONG, HONGMEI ZHANG, WENPING LUOrecombinant RNasin® ribonuclease inhibitor, 15 U AMV reverse transcriptase (high concentration), and 0.5 µg random primers (A3500; Promega, Madison, WI, USA). PCR was performed in a total volume of 25 µl containing 12.5 µl GoTaq® Green Master Mix

(382) Expression of hyperpolarization-activated cyclic nucleotide-gated channel isoforms in a canine model of atrial fibrillationExperimental and therapeutic medicineJuly 1, 2016He W, Zhang J, Gan T, Xu G, Tang B.A 1‑µg sample of reference RNA was reverse transcribed into cDNA using a reverse transcriptase kit (A3500; Promega Corporation, Madison, WI, USA). The design and synthesis details of the primers used are shown

(383) Pre-clinical efficacy and synergistic potential of the MDM2-p53 antagonists, Nutlin-3 and RG7388, as single agents and in combined treatment with cisplatin in ovarian cancerOncotargetJune 28, 2016Maryam Zanjirband, Richard J. Edmondson, John LunecScientific, UK). Total messenger RNA was converted to cDNA using the Promega Reverse Transcription System (A3500, Promega) as described by the manufacturer. Validated primers used (Sigma-Aldrich UK) are listed in

(384) Identification of L1 ORF2p sequence important to retrotransposition using Bipartile Alu retrotransposition (BAR)Nucleic Acids ResearchJune 2, 2016Claiborne M. Christian, Dawn deHaro, Kristine J. Kines, Mark Sokolowski, Victoria P. Belancioas described in (18,19). For detection of Actin and ORF2 RNA, Reverse Transcriptase System (Promega: A3500) was used according to manufacturer's instructions (Actin primers available upon request). Western blot analysis

(385) AIM2 inflammasome mediates Arsenic-induced secretion of IL-1 β and IL-18OncoimmunologyJune 1, 2016Zhang M, Qi Y, Li H, Cui J, Dai L, Frank JA, Chen J, Xu W, Chen G.and 18 S rRNA. The first-strand cDNA was synthesized using a Reverse Transcription System (Promega, A3500). PCR was performed on a Lightcycler 480 system (Roche) using a Power SYBR Green PCR Master kit (Invitrogen,

(386) HDAC 3-selective inhibitor RGFP966 demonstrates anti-inflammatory properties in RAW 264.7 macrophages and mouse precision-cut lung slices by attenuating NF-κB p65 transcriptional activityBiochemical pharmacologyMay 15, 2016Leus NG, van der Wouden PE, van den Bosch T, Hooghiemstra WT, Ourailidou ME, Kistemaker LE, Bischoff R, Gosens R, Haisma HJ, Dekker FJ.USA). For gene expression analysis, RNA was reverse transcribed using the Reverse Transcription kit (#A3500, Promega). Subsequently, 10 ng of cDNA was applied for each RT-qPCR, which was performed on an ABI Prism

(387) Attenuation of liver fibrosis by herbal compound 861 via upregulation of BMP-7/Smad signaling in the bile duct ligation model ratMolecular Medicine ReportsMay 1, 2016FEI HOU, RUIXIA LIU, XIAOYA LIU, LIJIAN CUI, YAN WEN, SONGBIAO YAN, CHENGHONG YINavian myeloblastosis virus reverse transcriptase, 1 µl Oligo dT‑Adaptor primer and 5 µg RNA sample (A3500; Promega Corp.). The RT reaction product was subsequently used for the amplification of cDNA using the

(388) Both diet and gene mutation induced obesity affect oocyte quality in miceScientific ReportsMay 1, 2016Yan-Jun Hou, Cheng-Cheng Zhu, Xing Duan, Hong-Lin Liu, Qiang Wang, Shao-Chen Sunmanufacturer’s instruction. A first cDNA strand was synthesized using a Reverse Transcription System A3500 (Promega, USA). Real-Time quantitative PCR was done using SYBR Premix Ex Taq (Takara) in a reaction

(389) C1q/TNF-related protein 1 links macrophage lipid metabolism to inflammation and atherosclerosisAtherosclerosisApril 26, 2016Xiao Qun Wang, Zhu Hui Liu, Lu Xue, Lin Lu, Jie Gao, Ying Shen, Ke Yang, Qiu Jing Chen, Rui Yan Zhang, Wei Feng Shen

(390) Effect of Dietary Nutrient Density on Small Intestinal Phosphate Transport and Bone Mineralization of Broilers during the Growing PeriodPLOS ONEApril 21, 2016Jianhui Li, Jianmin Yuan, Zhiqiang Miao, Zhigang Song, Yu Yang, Wenxia Tian, Yuming GuoAMV reverse transcriptase and an Oligo (dT)15 primer in the presence of recombinant RNasin ribonuclease inhibitor (A3500; Promega Corporation). The mRNAs of NaPi-IIb and NaPi-IIa were subjected to real-time PCR with β-actin

(391) Cis-vaccenic acid induces differentiation and up-regulates gamma globin synthesis in K562, JK1 and transgenic mice erythroid progenitor stem cellsEuropean journal of pharmacologyApril 5, 2016Aimola IA, Inuwa HM, Nok AJ, Mamman AI, Bieker JJ.et al. Page 5 Author Manuscript amounts of total RNA with Promega Reverse Transcription System (A3500) with a combination of random primers and oligo dT. γ-globin, β-globin and KLF1/EKLF gene expression

(392) siRNA-induced TRAF6 knockdown promotes the apoptosis and inhibits the invasion of human lung cancer SPC-A1 cellsOncology reportsApril 1, 2016He Z, Huang C, Lin G, Ye Y.manufacturer's protocol, and reverse-transcribed into cDNA using the Promega Reverse Transcription system A3500 (Promega) at 42°C for 15 min, at 95°C for 5 min, and at 4°C for 5 min. qRT-PCR assay was run in 20 µl

(393) Feedback regulation of apical progenitor fate by immature neurons through Wnt7-Celsr3-Fzd3 signallingNature communicationsMarch 4, 2016Wang W, Jossin Y, Chai G, Lien WH, Tissir F, Goffinet AM.mini kit (Qiagen). Reverse transcription was performed with a Promega reverse transcription system (A3500). qPCR was carried out using IQ SYBR green supermix system (Biorad). Primer sequences are provided in

(394) Effects of taxol resistance gene 1 expression on the chemosensitivity of SGC-7901 cells to oxaliplatinExperimental and therapeutic medicineMarch 1, 2016Liu L, Bai Z, Ma X, Wang T, Yang Y, Zhang Z.DNA synthesis was performed with reverse transcriptase using the Reverse Transcription system (cat. no., A3500; Promega Corporation, Madison, WI, USA) in accordance with the manufacturer's protocols. Total RNA (10

(395) miR-203 promotes proliferation, migration and invasion by degrading SIK1 in pancreatic cancerOncology ReportsMarch 1, 2016ZHI-GUO REN, SHU-XIAO DONG, PING HAN, JIAN QI(RT) system followed by PCR amplification in a 50-µl PCR system performed using an RT-PCR kit (cat no. A3500; Promega, Madison, WI, USA). Housekeeping gene glyceraldehyde‑3-phosphate dehydrogenase (GAPDH) was used

(396) Chemical Inhibition of Wild-Type p53-Induced Phosphatase 1 (WIP1/PPM1D) by GSK2830371 Potentiates the Sensitivity to MDM2 Inhibitors in a p53-Dependent MannerMolecular cancer therapeuticsMarch 1, 2016Esfandiari A, Hawthorne TA, Nakjang S, Lunec J.Quantitative real-time polymerase chain reaction (qRT-PCR) Complementary DNA was generated using the Promega Reverse Transcription System (A3500, Promega) as described by the manufacturer. qRT-PCR was carried out using SYBR® green RT-PCR master

(397) Differential Expression of Genes and DNA Methylation associated with Prenatal Protein Undernutrition by Albumen Removal in an avian modelScientific reportsFebruary 10, 2016Willems E, Guerrero-Bosagna C, Decuypere E, Janssens S, Buyse J, Buys N, Jensen P, Everaert N.manufacturer’s instructions. The RNA was transcribed into cDNA using the Reverse Transcription system (A3500, Promega, Madison, WI, USA). Denaturation was performed for 3 min at 80 °C followed by 45 min at 42

(398) Detection of influenza C virus but not influenza D virus in Scottish respiratory samplesJournal of Clinical VirologyJanuary 1, 2016Donald B. Smith, Eleanor R. Gaunt, Paul Digard, Kate Templeton, Peter Simmondsobtained between August 2006 and June 2008 were pooled in groups of 10 and reverse transcribed using the A3500 reverse transcription system (Promega) and random primers, as part of a previous study [4]. cDNA, derived

(399) The effects of enzymes and direct fed microbial combination on performance and immune response of broilers under a coccidia challengeJournal of Applied Animal NutritionJanuary 1, 2016Y. Dersjant-Li, K. Gibbs, A. Awati, K. C. Klasingto manufacturer’s instructions and reverse transcribed using Promega Reverse Transcription System (#A3500). Quantitative real-time PCR analysis was performed using a Roche Lightcycler (Roche Diagnostics #2

(400) Dehydroepiandrosterone Sulfate Stimulates Expression of Blood-Testis-Barrier Proteins Claudin-3 and -5 and Tight Junction Formation via a Gnα11-Coupled Receptor in Sertoli CellsPloS oneJanuary 1, 2016Papadopoulos D, Dietze R, Shihan M, Kirch U, Scheiner-Bobis G.transcription of the isolated mRNA was carried out by the Reverse Transcription System (Promega; cat. # A3500) according to the protocol of the provider. For PCR amplification a total of 1.5 μg of cDNA from the

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