Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(401) Microtubule-Associated Protein SBgLR Facilitates Storage Protein Deposition and Its Expression Leads to Lysine Content Increase in Transgenic Maize EndospermInternational Journal of Molecular SciencesDecember 12, 2015Chen Liu, Shixue Li, Jing Yue, Wenhan Xiao, Qian Zhao, Dengyun Zhu, Jingjuan Yusynthesis was carried out according to the manufacturer’s protocol of Reverse Transcription System (Promega A3500, Fitchburg, WI, USA). RT-PCR amplification was performed using SBgLR (ORF) specific primers (SBRT) (Table

(402) Core domain mutant Y220C of p53 protein has a key role in copper homeostasis in case of free fatty acids overloadBioMetalsDecember 1, 2015Mario Arciello, Alessia Longo, Carmela Viscomi, Concetta Capo, Antonio Angeloni, Luisa Rossi, Clara BalsanoTrizol reagent (Life Technologies, Carlsbad, CA, USA) and cDNA was prepared using Reverse Transcription System (A3500, Promega), according to the manufacturer’s protocol. Human PCR primers were designed IDT Integrated

(403) Transciptome analysis reveals flavonoid biosynthesis regulation and simple sequence repeats in yam (Dioscorea alata L.) tubersBMC GenomicsDecember 1, 2015Zhi-Gang Wu, Wu Jiang, Nitin Mantri, Xiao-Qing Bao, Song-Lin Chen, Zheng-Ming Taototal RNA per sample was treated with DNaseI (Takara), and reverse transcribed into cDNA using Promega A3500 reverse transcription system. Eight DEGs were selected for Quantitative real-time PCR (qRT-PCR) analysis

(404) Improved production of sublancin via introduction of three characteristic promoters into operon clusters responsible for this novel distinct glycopeptide biosynthesisMicrobial Cell FactoriesDecember 1, 2015Shengyue Ji, Weili Li, Abdul Rasheed Baloch, Meng Wang, Binyun CaoExtracted total RNA was reverse–transcribed into cDNA chain by a Reverse Transcription System Kit (#A3500; Promega, USA). Real–time PCR was carried out using Real time PCR Kit (#DRR041S; TaKaRa, Japan). The

(405) Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpressionMicrobial Cell FactoriesDecember 1, 2015Jingqi Chen, Gang Fu, Yuanming Gai, Ping Zheng, Dawei Zhang, Jianping Wenkit (Cat. Z3100, Promega). The cDNA chain was synthesized by using Reverse Transcription System (Cat. A3500, Promega). Real-time PCR was performed by using Real time PCR Kit (Cat. DRR041 S, TaKaRa). The target

(406) Clinical and Etiological Characteristics of Atypical Hand-Foot-and-Mouth Disease in Children from Chongqing, China: A Retrospective StudyBioMed Research InternationalNovember 26, 2015Xiang Yan, Zhen-Zhen Zhang, Zhen-Hua Yang, Chao-Min Zhu, Yun-Ge Hu, Quan-Bo LiuSpin Kits (Qiagen, Hilden, Germany) and cDNA was synthesized by using Reverse Transcription System A3500 (Promega, Madison, WI, USA) according to the manufacturer's instructions. Nested-PCR was performed to

(407) Scutellarin regulates microglia-mediated TNC1 astrocytic reaction and astrogliosis in cerebral ischemia in the adult ratsBMC neuroscienceNovember 25, 2015Fang M, Yuan Y, Rangarajan P, Lu J, Wu Y, Wang H, Wu C, Ling EA.20 μl reaction mixture containing 0.5 μg of oligo (dT) primer (Promega, Madison, WI, USA; Cat. No. A3500), and 15U of AMV Reverse Transcriptase (Promega, Madison, WI, USA; Cat. No. M5314). The reaction was

(408) Seed-Specific Expression of the Arabidopsis AtMAP18 Gene Increases both Lysine and Total Protein Content in MaizePLOS ONENovember 18, 2015Yujie Chang, Erli Shen, Liuying Wen, Jingjuan Yu, Dengyun Zhu, Qian ZhaoRT-PCR, 3 μg of total mRNA was used for cDNA synthesis according to the manufacturer's protocol (Promega A3500, Madison, WI, USA). Amplifications were performed in a total volume of 20 μL containing 100 ng cDNA,

(409) Cyclic RNA hsa‑circ‑000595 regulates apoptosis of aortic smooth muscle cellsMolecular medicine reportsNovember 1, 2015Zheng C, Niu H, Li M, Zhang H, Yang Z, Tian L, Wu Z, Li D, Chen X.template to prepare cDNA (Reverse Transcription System, Promega Corporation, Madison, WI, USA; cat. no. A3500). The mRNA expression of target genes (hsa-circ-000595 and miR-19a) was quantified using SYBR Premix

(410) A quantitative proteomics study on olfactomedin 4 in the development of gastric cancerInternational Journal of OncologyNovember 1, 2015XIAOPING RAN et al.to the manufacturer's instructions. First-strand cDNA was synthesized from 2 µg of total RNA, using A3500 Reverse transcription system (Promega). RT-PCR was performed on an ABI 7900HT system using the Taq-Man

(411) SATB1 Promotes Pancreatic Cancer Growth and Invasion Depending on MYC ActivationDigestive diseases and sciencesNovember 1, 2015Chen Z, Li Z, Li W, Zong Y, Zhu Y, Miao Y, Xu Z.lines using Trizol reagent (Invitrogen Life Technologies). Total RNA (1 μg) was reverse-transcribed by A3500 AMV RT-PCR (reverse transcription polymerase chain reaction) system (Promega, Madison, WI, USA). The

(412) The Unreliability of MTT Assay in the Cytotoxic Test of Primary Cultured Glioblastoma CellsExperimental NeurobiologySeptember 30, 2015Hwa Yeon Jo, Yona Kim, Hyung Woo Park, Hyo Eun Moon, Seongtae Bae, JinWook Kim, Dong Gyu Kim, Sun Ha Paekethanol treatment. Total RNA was reverse transcribed using the Reverse Transcription System (Promega, A3500, WI, USA) as per instructions. The primers used were as follows: Bax, (forward: 5'-AGGATGCGTCCACCAAGAAG-3')

(413) Single-cell Migration Chip for Chemotaxis-based Microfluidic Selection of Heterogeneous Cell PopulationsScientific ReportsSeptember 1, 2015Yu-Chih Chen, Steven G. Allen, Patrick N. Ingram, Ronald Buckanovich, Sofia D. Merajver, Euisik YoonRNA was eluted in 9 μL. The cDNA was prepared using the Reverse Transcription System (Promega, Cat. A3500) according to the standard protocol using Oligo(dT) primers and the entirety of the harvested RNA (~7

(414) A Novel, Highly Selective RT-QPCR Method for Quantification of MSRV Using PNA Clamping Syncytin-1 (ERVWE1)Molecular BiotechnologySeptember 1, 2015Grzegorz Machnik, Estera Skudrzyk, Łukasz Bułdak, Krzysztof Łabuzek, Jarosław Ruczyński, Magdalena Alenowicz, Piotr Rekowski, Piotr Jan Nowak, Bogusław Okopieńthe reverse transcriptase enzyme. As it is stated in Promega’s instruction for use of their product # A3500 “Choose Oligo(dT)15 when priming at the 3′poly(A) region is desired. Choose Random Primers when priming

(415) Heart‐Specific Knockout of the Mitochondrial Thioredoxin Reductase ( Txnrd2 ) Induces Metabolic and Contractile Dysfunction in the Aging MyocardiumJournal of the American Heart AssociationJuly 21, 2015Claudia Kiermayer et al.TriFast reagent (30-2010; peqLab) and cDNA was generated by using a Reverse Transcription System kit (A3500; Promega Corp) by following the manufacturers’ protocols. Quantitative real-time (RT)-polymerase chain reaction (PCR

(416) Long-Term Administration of Neuropeptide Y in the Subcutaneous Infusion Results in Cardiac Dysfunction and Hypertrophy in RatsCellular Physiology and BiochemistryJuly 6, 2015Rong Zhang, Huifang Niu, Xiaohui Kang, Tao Ban, Hong Hong, Jing AicDNA synthesis was performed according to the manufacturer's instructions (Reverse Transcription System, Cat#A3500, Promega) and as described previously [33]. The SYBR Green PCR Master Mix Kit (Applied Biosystems, Cat#4309155)

(417) Orostachys japonicus inhibits the expression of MMP-2 and MMP-9 mRNA and modulates the expression of iNOS and COX-2 genes in human PMA-differentiated THP-1 cells via inhibition of NF-κB and MAPK activationMolecular medicine reportsJuly 1, 2015Kim YI, Park SW, Yoon YK, Lee KW, Lee JH, Woo HJ, Kim Y.with 1 µg total RNA and transcribed to cDNA using a reverse‑transcription system with random hexamers (A3500; Promega) according to the manufacturer's instructions. The sequences for gene‑specific primers (Bioneer,

(418) Mechanisms of cytotoxicity induced by the anesthetic isoflurane: the role of inositol 1,4,5-trisphosphate receptorsGenetics and Molecular ResearchJune 26, 2015W.-H. Zhai, J. Zhao, S.-P. Huo, X.-G. Chen, Y.-D. Li, Z.-L. Zhang, L.-L. Yu, S. Song, Q.-J. Wangof RNA were determined by the absorbance values at 260 nm. A reverse transcription system (Promega A3500) was used to reverse transcribe RNA to cDNA. Reverse transcription reactions were performed in a 20-μL

(419) Dynamic expressions of monocyte chemo attractant protein-1 and CC chamomile receptor 2 after balloon injury and their effects in intimal proliferationBiomedical engineering onlineJune 11, 2015Huang Z, Li Y, Niu L, Xiao Y, Pu X, Zheng H, Qian M.(Shanghai Analysis Instrument Factory Type 751-GW), TRIZOL (Invitrogen), Reverse Transcription Kit (Promega A3500), PCR Reagents (Takara Company), Immune Tissue Staining Kit, and Elastic Fiber Staining Kit. Animal

(420) Synapsin IIb as a functional marker of submissive behaviorScientific reportsMay 22, 2015Nesher E, Koman I, Gross M, Tikhonov T, Bairachnaya M, Salmon-Divon M, Levin Y, Gerlitz G, Michaelevski I, Yadid G, et al.reverse transcribed in a final volume of 20 μl, using a reverse transcription system (Promega, USA, A3500) with random primers, according to the manufacturer’s instructions. mRNA levels were analyzed by quantitative real time PCR

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