Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(301) Protective effects of heme oxygenase-1 against severe acute pancreatitis via inhibition of tumor necrosis factor-α and augmentation of interleukin-10BMC GastroenterologyDecember 1, 2017Fei-hu Zhang, Yu-han Sun, Kai-liang Fan, Xiao-bin Dong, Ning Han, Hao Zhao, Li Kongaliquots of 5 μg of total RNA were reverse-transcribed using the first-strand cDNA synthesis kit (Promega A3500, Madison, WI) [3]. The cDNA was then amplified by polymerase chain reaction using specific primers for

(302) Effect of Salicylic Acid on the Gene Transcript and Protein Accumulation of Flavonoid Biosynthesis-related Enzymes in Vitis vinifera Cell Suspension CulturesHortScienceDecember 1, 2017Huiling Wang, Wei Wang, Weidong Huang, Haiying Xuaccording to the manufacturer’s instruction of avian myeloblastosis virus reverse transcriptase (Promega A3500; Promega, Madison, WI). The mRNA expression patterns of the four selected genes including VvCHS, VvCHI, VvDFR,

(303) The pennycress (Thlaspi arvense L.) nectary: structural and transcriptomic characterizationBMC Plant BiologyDecember 1, 2017Jason B. Thomas, Marshall E. Hampton, Kevin M. Dorn, M. David Marks, Clay J. Carterisolated as described for nectaries above and cDNA was created with the Promega Reverse Transcription System (A3500, Promega Corp., Madison, WI, USA) according to the manufacturer’s instructions. Negative controls without reverse

(304) Effect of catalpol on remyelination through experimental autoimmune encephalomyelitis acting to promote Olig1 and Olig2 expressions in miceBMC Complementary and Alternative MedicineDecember 1, 2017Tao Yang, Qi Zheng, Su Wang, Ling Fang, Lei Liu, Hui Zhao, Lei Wang, Yongping Fan(Cambridge, UK). Trizol reagent was purchased from Invitrogen (Carlsbad, CA, USA). Reverse transcription kit A3500 was purchased from Promega (Madison, Wisconsin, USA). Taq DNA polymerase, buffer and DNA molecular weight

(305) FOXO1 is crucial in glioblastoma cell tumorigenesis and regulates the expression of SIRT1 to suppress senescence in the brainMolecular Medicine ReportsNovember 23, 2017Han Yan, Anhua Wu(Invitrogen; Thermo Fisher Scientific, Inc.), following which a reverse transcription system (Promega A3500; Thermo Fisher Scientific, Inc.) was used for the synthesis of first strand cDNA. Quantitation of the

(306) Beneficial effect of resveratrol on α‑naphthyl isothiocyanate‑induced cholestasis via regulation of the FXR pathwayMolecular Medicine ReportsNovember 14, 2017Lili Ding, Binfeng Zhang, Jinmei Li, Li Yang, Zhengtao Wangthe NM_011850 NM_007824 NM_024264 NM_010012 NM_008003 NM_021022 NM_008375 NM_013806 NM_029600 NM_178933 NM_010927 NM_011198 NM_013693 NM_031168 NM_008361 NM_010493 NM_011333 NM_007475 manufacturer's instructions. RNA concentrations were equalized and converted to cDNA using an A3500 reverse transcription system (Promega). Gene expressions were measured by qRT‑PCR (ABI 7500, Applied

(307) CHAF1A, the largest subunit of the chromatin assembly factor 1 complex, regulates the growth of H1299 human non-small cell lung cancer cells by inducing G0/G1 cell cycle arrestExperimental and therapeutic medicineNovember 1, 2017Liu T, Wei J, Jiang C, Wang C, Zhang X, Du Y, Li J, Zhao H.and reversely transcribed to first-strand cDNA using the Reverse Transcription system (catalog no. A3500; Promega Corporation, Madison, WI, USA). DNase (Tiangen Biotech Co., Ltd., Beijing, China) was employed

(308) Overexpression of ATP5b promotes cell proliferation in asthmaMolecular medicine reportsNovember 1, 2017Zuo J, Lei M, Wen M, Chen Y, Liu Z.et al (30). Five micrograms of total RNA from each sample was reverse transcribed to cDNA using an A3500 RT system (Promega Corporation, Madison, WI, USA). The following ZUO et al: ATP5b PROMOTES CELL

(309) Efficient crude multi-enzyme produced by Trichoderma reesei using corncob for hydrolysis of lignocellulose3 BiotechOctober 1, 2017Fengchao Jiang, Lijuan Ma, Rui Cai, Qing Ma, Gaojie Guo, Liping Du, Dongguang Xiaowhich contained a blend of oligo (dT) and random hexamer primers. qPCRs were performed in a real-time PCR system (A3500, Promega). The reaction system with a total volume of 20 ll contained 2 ll template cDNA, 10 ll SYBR@Premix

(310) Bombyx mori hemocyte extract has anti-inflammatory effects on human phorbol myristate acetate-differentiated THP‑1 cells via TLR4-mediated suppression of the NF-κB signaling pathwayMolecular medicine reportsOctober 1, 2017Kim YI, Choi KH, Kim SR, Goo TW, Park SW.first‑strand cDNA synthesis, 1 µg total RNA was transcribed to cDNA using a reverse‑transcription system with random hexamers (A3500; Promega Corporation) according to the manufacturer's protocol. RT‑qPCR was performed on a StepOnePlus Real‑Time PCR system

(311) IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity RiskEBioMedicineOctober 1, 2017Zou Y, Lu P, Shi J, Liu W, Yang M, Zhao S, Chen N, Chen M, Sun Y, Gao A, et al.instructions. 1 μg of RNA was reverse transcribed to cDNA with a reverse transcription kit (Promega, A3500). Real-time PCR was carried out on the QuantStudio Dx system (Thermo Fisher Scientific Inc.) using SYBR

(312) Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replicationPLoS pathogensOctober 1, 2017Wang T, Wang B, Huang H, Zhang C, Zhu Y, Pei B, Cheng C, Sun L, Wang J, Jin Q, et al.total RNA was reverse transcribed in a 20 μl volume using Improm-IITM reverse transcriptase (Promega, A3500) following the manufacturer’s instructions. Real-time PCR was performed using 1 μl cDNA as the template

(313) Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and RewardNeuronSeptember 27, 2017Laguesse S, Morisot N, Shin JH, Liu F, Adrover MF, Sakhai SA, Lopez MF, Phamluong K, Griffin WC 3rd, Becker HC, et al.,Thermo Fisher Scientific,23225 HIV-1 p24 antigen ELISA kit,ZeptoMetrix Corporation,0801111 Reverse Transcription System,Promega,A3500 Deposited Data,Deposited Data,Deposited Data Raw data associated with this paper,N/A,http://dx.doi.org/10.17632/xzmp3zffz7.1 Experimental Models

(314) Overexpression of long non‑coding RNA colon cancer‑associated transcript 2 is associated with advanced tumor progression and poor prognosis in patients with colorectal cancerOncology LettersSeptember 25, 2017Junling Zhang, Yong Jiang, Jing Zhu, Tao Wu, Ju Ma, Chuang Du, Shanwen Chen, Tengyu Li, Jinsheng Han, Xin Wangall samples ranged between 6.0 and 10.0. cDNA was synthesized from 500 ng total RNA using the Promega A3500 Reverse Transcription System kit (Promega Corporation, Madison, WI, USA) with random hexamer primers

(315) RNAi targeting Nogo Receptor enhanced survival and proliferation of murine retinal ganglion cells during N-methyl-D-aspartate-induced optic nerve crushOncotargetSeptember 12, 2017Kun Zeng, Bo Zhong, Xiao-Li Shen, Min Fang, Bao-Tao Lin, Da-Hui Matranscription was performed according to the protocol provided by a RNA reverse transcription kit (A3500, Promega, Madison, WI, USA).For quantitative PCR, the protocol included pre-denaturation at 95°C for

(316) Mettl3-mediated m6A regulates spermatogonial differentiation and meiosis initiationCell ResearchSeptember 1, 2017Kai Xu et al.M6101), 1 µg total RNA reverse-transcribed into cDNAs using the reverse Transcription System (Promega, A3500). qRT-PCR was performed using a SYBR Premix Ex Taq kit (TaKaRa, RR420A) on Agilent Stratagene Mx3005P.

(317) Towards an understanding of the molecular basis of effective RNAi against a global insect pest, the whitefly Bemisia tabaciInsect Biochemistry and Molecular BiologySeptember 1, 2017Yuan Luo, Qingguo Chen, Junbo Luan, Seung Ho Chung, Joyce Van Eck, R. Turgeon, Angela E. Douglasof whitefly genes were prepared with oligo (dT) primers using the Reverse Transcription System (Cat# A3500, Promega, Madison, USA) following the manufacturer's instructions. Candidate osmoregulatory genes, comprising

(318) Differential gene expression in leaf tissues between mutant and wild-type genotypes response to late leaf spot in peanut (Arachis hypogaea L.)PLOS ONEAugust 25, 2017Suoyi Han et al.RNA were used to synthesize the first-strand cDNA using a reverse-transcription system (Promega, USA, A3500) according to the manufacturer’s instructions. Quantitative real-time PCR was performed on the Eppendorf

(319) Effect and mechanism of inhibition of PI3K/Akt/mTOR signal pathway on chronic neuropathic pain and spinal microglia in a rat model of chronic constriction injuryOncotargetAugust 8, 2017Jian-Rong Guo, Huan Wang, Xiao-Ju Jin, Dong-Lin Jia, Xun Zhou, Qiang TaoPCR of the total RNA was conducted following the steps suggested by the Reverse Transcription System A3500 from Promega Company (USA). The PCR protocol included one cycle of initial denaturation (95°C, 3 minutes),

(320) Revealing potential long non-coding RNA biomarkers in lung adenocarcinoma using long non-coding RNA-mediated competitive endogenous RNA networkBraz. J. Med. Biol. ResAugust 7, 2017T-G. Zhu, X. Xiao, Q. Wei, M. Yue, L-X. Zhangrelying on the manufacturer's protocol. Reverse transcription reaction was implemented by means of A3500 reverse transcription system kit (Promega, USA) according to the manufacturer's protocol. We used the

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