Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(361) Suppression of LPS-induced tau hyperphosphorylation by serum amyloid AJournal of NeuroinflammationDecember 1, 2016Jin Liu, Ding Wang, Shu-Qin Li, Yang Yu, Richard D. YeOne microgram of the RNA was used for reverse transcription using the Reverse Transcription System A3500 kit (Promega, Madison, WI). The complementary DNA (cDNA) was subsequently subjected to Real-Time PCR

(362) MLL1 promotes cervical carcinoma cell tumorigenesis and metastasis through interaction with β-cateninOncoTargets and TherapyNovember 16, 2016Rong Qiang, Na Cai, Xiaobin Wang, Lin Wang, Ke Cui, Xiang Wang, Xu Limanufacturer’s protocol. Then, first-strand cDNA was synthesized through Reverse Transcription System (A3500; Promega Corporation, Fitchburg, WI, USA). The relative expression level of mRNA was confirmed by quantitative reverse

(363) Positive Regulation of Decidualization by l-Type Amino Acid Transporter 1 (lat1) in Pregnant MiceNutrientsNovember 5, 2016Wang X, Tan D, Ma J, Liang H, Zhang Q, Tan Y, Wang J, Luo W.Recombinant RNasin® Ribonuclease Inhibitor, 15 U AMV Reverse Transcriptase (High Conc.), and 0.5 µg random primers (A3500, Promega, Madison, WI, USA). The PCR was performed in a total volume of 25 µL containing 12.5 µL GoTaq® Green Master Mix

(364) Integrated analysis of long non-coding RNA-associated ceRNA network reveals potential lncRNA biomarkers in human lung adenocarcinomaInternational Journal of OncologyNovember 1, 2016Jing Sui et al.NanoDrop 2000 spectrometer (Thermo Fisher Scientific, Waltham, Ma, USA). Reverse transcription reactions using A3500 reverse transcription system kit (Promega, Madison, Wi, USA) was conducted in two steps according to

(365) Fibroblast growth factor receptor (FGFR) alterations in squamous differentiated bladder cancer: a putative therapeutic target for a small subgroupOncotargetNovember 1, 2016Philipp H. Baldia, Angela Maurer, Timon Heide, Michael Rose, Robert Stoehr, Arndt Hartmann, Sarah V. Williams, Margaret A. Knowles, Ruth Knuechel, Nadine T. GaisaFoster City, USA) according to the manufacturer's instructions. cDNA was synthesized with Promega Kit A3500 (Promega, Madison, WI, USA). FGFR3-TACC3 fusions were assessed by PCR, adapted from Williams et al.

(366) Effect of an immunomodulatory regimen for cancer prevention: A case reportMolecular and Clinical OncologyNovember 1, 2016Wenbo Wang, Wei Wang, Bing Liu, Zhuoyao Suo, Lin Zhou, Guangxian Liusubsequently reverse-transcribed into complementary (c)DNA using a reverse-transcription system (cat. no. A3500; Promega Corp., Madison, WI, USA). Reagents were used and the RT protocol was performed according to

(367) Roles of 1,25(OH)2D3 and Vitamin D Receptor in the Pathogenesis of Rheumatoid Arthritis and Systemic Lupus Erythematosus by Regulating the Activation of CD4+ T Cells and the PKCδ/ERK Signaling PathwayCellular Physiology and BiochemistryOctober 25, 2016Xiao-Jie He, Yan Ding, Wei Xiang, Xi-Qiang Dangwas selected for reverse transcription in accordance with instructions on Reverse Transcription System A3500 reverse transcription kit purchased from Promega Compay. According to gene sequences released publically by

(368) Overexpression of LRIG1 regulates PTEN via MAPK/MEK signaling pathway in esophageal squamous cell carcinomaExperimental and therapeutic medicineOctober 1, 2016Jiang X, Li H.synthesized from 1 µg RNA in a 20 µl reaction mixture using the Reverse Transcription System (cat. no. A3500; Promega Corporation, Madison, WI, USA). cDNA was amplified using the following primers: GAPDH forward,

(369) Effects of age on intestinal phosphate transport and biochemical values of broiler chickensAsian-Australasian Journal of Animal SciencesSeptember 27, 2016Jianhui Li, Jianmin Yuan, Zhiqiang Miao, Yuming GuoAMV reverse transcriptase and an Oligo (dT) 15 primer in the presence of recombinant RNasin ribonuclease inhibitor (A3500; Promega Corporation, USA). The mRNA of NaPi-IIb was subjected to real-time polymerase chain reaction

(370) miR-20a regulates proliferation, differentiation and apoptosis in P19 cell model of cardiac differentiation by targeting SmoothenedBiology OpenSeptember 15, 2016Feng Ai, Yanwei Zhang, Bangtian Pengmanufacturer's protocol, and reverse transcribed into cDNA by using the AMV reverse transcriptase kit (Promega A3500; Madison, WI, USA). miR-20a expression was determined by TaqMan miRNA assays (Ambion, Foster City, CA,

(371) Large intergenic non-coding RNA-ROR reverses gemcitabine-induced autophagy and apoptosis in breast cancer cellsOncotargetSeptember 13, 2016Chen YM, Liu Y, Wei HY, Lv KZ, Fu PF.Experiments were performed in accordance with procedures provided by the Reverse Transcription System A3500 (Promega Company, USA). The reaction conditions were: pre-denaturation at 95°C for 15 min for 1 cycle,

(372) NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activationNature communicationsSeptember 7, 2016Yang M, Chen S, Du J, He J, Wang Y, Li Z, Liu G, Peng W, Zeng X, Li D, et al.using Trizol Kit (Invitrogen), and reverse-transcribed using reverse transcription system (Promega, A3500). Quantitative PCR was conducted using SYBR green-based detection. The expression level of the genes

(373) Ribosomal proteins produced in excess are degraded by the ubiquitin-proteasome systemMolecular biology of the cellSeptember 1, 2016Sung MK, Reitsma JM, Sweredoski MJ, Hess S, Deshaies RJ.CA). cDNA for reverse transcription (RT)-PCR was generated using the Reverse Transcription System (A3500; Promega, Madison, WI). The amounts of RPL26A and ACT1 mRNA were analyzed by quantitative real-time RT-PCR

(374) ALS disrupts spinal motor neuron maturation and aging pathways within gene co-expression networksNature neuroscienceSeptember 1, 2016Ho R, Sances S, Gowing G, Amoroso MW, O'Rourke JG, Sahabian A, Wichterle H, Baloh RH, Sareen D, Svendsen CN.the fetal spinal cord samples. RT-qPCR was performed using the Reverse Transcription System (Promega A3500), SYBR Select Master Mix (Life Technologies 4472908), and the BIO-RAD CFX384 Real-Time System. Primer

(375) PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancerOncology lettersSeptember 1, 2016Lian S, Meng L, Xing X, Yang Y, Qu L, Shou C.Trizol reagent (Thermo Fisher Scientific, Inc.). RT was conducted using a Reverse Transcription System (#A3500; Promega Corporation, Madison, WI, USA) according to the manufacturer's instructions with the following

(376) Regulation of actin dynamics by WNT-5A: Implications for human airway smooth muscle contraction3.2 Airway Cell Biology and ImmunopathologySeptember 1, 2016Tim Koopmans, Kuldeep Kumawat, Andrew Halayko, Reinoud Gosensmanufacturer’s instructions. Equal amounts of cDNA were synthesized using AMV reverse transcriptase (Promega, #A3500) and diluted 15 times with RNAse-free ddH2O. Quantitative real-time PCR was performed on an Illumina

(377) Age-Dependent Changes of Monocarboxylate Transporter 8 Availability in the Postnatal Murine RetinaFrontiers in Cellular NeuroscienceAugust 26, 2016Yoshiyuki Henning, Karol Szafranskimanufacturer’s instructions. Complementary DNA (cDNA) was synthesized using AMV Reverse Transcriptase (Promega; catalog no. A3500) primed by random oligomers. We reverse-transcribed 0.25 µg RNA in 20-µl reactions, as recommended by

(378) Long non-coding RNA AK027294 involves in the process of proliferation, migration, and apoptosis of colorectal cancer cellsTumor BiologyAugust 1, 2016Hui Niu, Zhaoyang Hu, Hui Liu, Guoliang Hu, Bo Yang, Shixiu Wu, Fang Litemplate to prepare cDNA (Reverse Transcription System, Promega Corporation, Madison, WI, USA; cat. no. A3500). The messenger RNA (mRNA) expression of target genes was quantified using SYBR Premix EX Taq (Takara

(379) Regulation of actin dynamics by WNT-5A: implications for human airway smooth muscle contractionScientific reportsJuly 29, 2016Koopmans T, Kumawat K, Halayko AJ, Gosens R.manufacturer’s instructions. Equal amounts of cDNA were synthesized using AMV reverse transcriptase (Promega, #A3500) and diluted 15 times with RNAse-free ddH2O. Quantitative real-time PCR was performed on an Illumina

(380) Activation of the vitamin D receptor selectively interferes with calcineurin-mediated inflammation: a clinical evaluation in the abdominal aortic aneurysmLaboratory investigation; a journal of technical methods and pathologyJuly 1, 2016Nieuwland AJ, Kokje VB, Koning OH, Hamming JF, Szuhai K, Claas FH, Lindeman JH.(Campro Scientific, Veenendaal, The Netherlands) and glass beads.9 Copy DNA was prepared using kit #A3500 (Promega, Leiden, The Netherlands) and quantitative real‐time PCR (Taqman system) analysis was performed

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