Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(281) PF-2341066 combined with celecoxib promotes apoptosis and inhibits proliferation in human cholangiocarcinoma QBC939 cellsExperimental and Therapeutic MedicineMarch 20, 2018Chen Chen, Dinghua Yang, Qinghua Zeng, Liang Luo, Chengzhi Caitotal of 1 µg RNA was reverse transcribed at 42˚C using a Reverse Transcription System kit (cat. no. A3500; Promega Corp., Madison, WI, USA) in accordance with the manufacturers' instructions. qPCR was performed

(282) KCC2-GABAA pathway correlates with the analgesic effect of electro-acupuncture in CCI ratsMolecular Medicine ReportsMarch 16, 2018Si‑Si Li, Wen‑Zhan Tu, Cheng‑Qian Jia, Xia Jiang, Xin‑Ru Qian, Guan‑Hu Yang, Qi‑Miao Hu, Wen‑Ci Chen, Bin Lu, Song‑He Jianggroup). Total RNA (1 µg) was reverse transcribed using High Capacity RNA-to-cDNA Master Mix (cat. no. A3500; Promega Corporation, Madison, WI, USA). Real-time amplification was conducted using SYBR Green Supermix

(283) Effect of electro-acupuncture on the BDNF-TrkB pathway in the spinal cord of CCI ratsInternational Journal of Molecular MedicineMarch 13, 2018Wen-Zhan Tu, Si-Si Li, Xia Jiang, Xin-Ru Qian, Guan-Hu Yang, Peng-Peng Gu, Bin Lu, Song-He Jiang(Invitrogen, Carlsbad, CA, USA). First-Strand cDNA was synthesised by a reverse transcriptase kit (A3500; Promega, Madison, WI, USA) according to the manufacturer's instructions. Real-time amplification, using

(284) Nuclear GSK3β induces DNA double-strand break repair by phosphorylating 53BP1 in glioblastomaInternational journal of oncologyMarch 1, 2018Yang Y, Lei T, Du S, Tong R, Wang H, Yang J, Huang J, Sun M, Wang Y, Dong Z.by reverse transcription-polymerase chain reaction using a Reverse Transcript System kit (Cat. no. A3500, Promega), and then inserted into the pBluescript bacterial expression vector, which was purchased from

(285) Effect of a synthetic inhibitor of urokinase plasminogen activator on the migration and invasion of human cervical cancer cells in vitroMolecular medicine reportsMarch 1, 2018Wang X, Jiang Z, An J, Mao X, Lin F, Sun P.of 1 µg DNase I-treated RNA were reverse-transcribed to cDNA using the reverse transcription system A3500 (Promega Corporation, Madison, WI, USA). The PCR primer sets were synthesized by Takara Biotechnology

(286) Effect of Paclitaxel+Hirudin on the TLR4-MyD88 Signaling Pathway During Inflammatory Activation of Human Coronary Artery Smooth Muscle Cells and Mechanistic AnalysisCellular Physiology and BiochemistryFebruary 28, 2018Hongmei Li, Xian Wang, Anlong XuBeijing, China. The reverse transcription kits used were Promega products (Madison, WI, USA; art. no. A3500). The obtained cDNA was diluted 5 times with 80 μl RNA-free water. The 25 μl reaction system used contained

(287) Chrysophanol inhibits proliferation and induces apoptosis through NF-κB/cyclin D1 and NF-κB/Bcl-2 signaling cascade in breast cancer cell linesMolecular Medicine ReportsJanuary 17, 2018Li Ren, Zhouping Li, Chunmei Dai, Danyu Zhao, Yanjie Wang, Chunyu Ma, Chun Liureverse transcription of total RNA into cDNA was performed using the Reverse Transcription System (A3500; Promega Corp., Madison, WI, USA) and PrimerScript RT Master Mix kit (Takara Bio, Dalian, China). Briefly,

(288) Beraprost Sodium, a Stable Analogue of PGI2, Inhibits the Renin-Angiotensin System in the Renal Tissues of Rats with Chronic Renal FailureKidney and Blood Pressure ResearchJanuary 9, 2018Xin-Fang Wang, Bei-Hao Zhang, Xiao-Qing Lu, Pei Wangperformed using total RNA based on the experimental procedure described for a Reverse Transcription System A3500 (Promega, Wisconsin, USA). After reverse transcription, extracted cDNA was diluted with a certain amount

(289) Intermittent Hypoxia Disrupts Glucose Homeostasis in Liver Cells in an Insulin-Dependent and Independent MannerCellular Physiology and BiochemistryJanuary 9, 2018Chen Juan Gu, Hua Hua Yi, Jing Feng, Zhi Guo Zhang, Jun Zhou, Li Na Zhou, Jian Ping Zhou, Min Li, Qing Yun Li(Invitrogen). First-strand cDNA was synthesized from total RNA using the Reverse Transcription System (A3500, Promega, Madison, WI, USA). Gene expression was analyzed by real-time PCR with 2−ΔΔCt relative quantitative method

(290) Modified Si-Ni-San Decoction Ameliorates Central Fatigue by Improving Mitochondrial Biogenesis in the Rat HippocampusEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2018Han C, Li F, Liu Y, Ma J, Yu X, Wu X, Zhang W, Li D, Chen D, Dai N, et al.then reverse-transcribed 1 μg of total RNA to 50 μl of cDNA with the Reverse Transcription System (A3500, Promega). We designed primers with Primer-BLAST (https://www.ncbi.nlm.nih.gov/tools/primer-blast/)

(291) Progressive Degeneration and Inhibition of Peripheral Nerve Regeneration in the SOD1-G93A Mouse Model of Amyotrophic Lateral SclerosisCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018Deng B, Lv W, Duan W, Liu Y, Li Z, Ma Y, Zhang G, Song X, Cui C, Qi X, et al.transcription using avian myeloblastosis virus (AMV) reverse transcriptase and random primers (Promega, A3500), quantitative real-time PCR (Agilent Technologies, Santa Clara, USA) was performed using SYBR Green

(292) Proteomics Based Identification of Autotaxin As An Anti-Hepatitis B Virus Factor and a Promoter of Hepatoma Cell Invasion and MigrationCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018She S, Yang M, Hu H, Hu P, Yang Y, Ren H.produced by reverse-transcription of the total RNA by employing the Promega Reverse Transcription System A3500. An ABI 7900HT System (Applied Biosystems, Foster City, CA) was used for quantitative RT-PCR using gene-specific

(293) Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor NeuronsFrontiers in neuroscienceJanuary 1, 2018Seminary ER, Sison SL, Ebert AD.differentiation. 2 μg of RNA was used to generate cDNA according to manufacturer instructions (Promega, cat. A3500). qPCR for HspB1, HspB8, BAG3, and GAPDH was performed using 20 ng cDNA and a Bio-Rad Connect96 Thermocycler

(294) Overexpression of GRK3, Promoting Tumor Proliferation, Is Predictive of Poor Prognosis in Colon CancerDisease MarkersDecember 28, 2017Tao Jiang, Chun Yang, Liyuan Ma, Zehua Wu, Ling Ye, Xiaoqiang Ma, Hai Li, Junwei Fan, Yinxue Yang(Thermo Scientific, USA). First-strand cDNA was synthesized from one microgram of total RNA using the A3500 RT-PCR System (Promega, USA). Quantitative real-time PCR was performed on a Mastercycler® ep realplex

(295) Macrophage migration inhibitory factor mediates protease-activated receptor 4-induced bladder pain through urothelial high mobility group box 1Physiological ReportsDecember 21, 2017Fei Ma, Dimitrios E. Kouzoukas, Katherine L. Meyer-Siegler, David E. Hunt, Lin Leng, Richard Bucala, Pedro L. VeraThermoFisher Scientific, Grand Island, NY), DNA removed by DNase, and reversed transcribed to cDNA (A3500, Promega, Madison, WI). SYBR green (4472903, ThermoFisher Scientific, Grand Island, NY) was utilized

(296) Effect of early dietary energy restriction and phosphorus level on subsequent growth performance, intestinal phosphate transport, and AMPK activity in young broilersPLOS ONEDecember 14, 2017Zhiqiang Miao, Guixian Zhang, Junzhen Zhang, Jianhui Yang, Jianhui YangUSA) using a 15-mer oligo (dT) primer in the presence of recombinant RNasin ribonuclease inhibitor (A3500; Promega, Madison, WI, USA). Real-time PCR of NaPi-IIb was performed with β-actin as the internal control.

(297) Regulation of phosphate transport and AMPK signal pathway by lower dietary phosphorus of broilersOncotargetDecember 8, 2017Zhiqiang Miao, Yan Feng, Junzhen Zhang, Wenxia Tian, Jianhui Li, Yu YangUSA) using a 15-mer oligo (dT) primer in the presence of recombinant RNasin ribonuclease inhibitor (A3500; Promega, Madison, WI, USA). Real-time PCR of NaPi-IIb, NaPi-IIa were performed with β-actin as the

(298) CXCL5 as an autocrine or paracrine cytokine is associated with proliferation and migration of hepatoblastoma HepG2 cellsOncology lettersDecember 1, 2017Yang Y, Hou J, Shao M, Zhang W, Qi Y, E S, Wang S, Sui H, Meng D, Wang B, et al.Scientific, Inc.) and first‑strand cDNA was synthesized using Reverse Transcription System (cat. no. A3500; Promega Corporation, Madison, WI, USA). PCR primers were synthesized by Sangon Biotech Co., Ltd. (Shanghai,

(299) Thiamine antagonists trigger p53-dependent apoptosis in differentiated SH-SY5Y cellsScientific ReportsDecember 1, 2017Sergiy Chornyy, Yulia Parkhomenko, Nataliya Chorna(Thermofisher Sci). RT–PCR cDNA was obtained using the Reverse Transcription System kit (Promega, A3500) followed by PCR in a total volume of 25 µL PCR Master Mix (Promega, M7502) as previously described

(300) The Expression of Formyl Peptide Receptor 1 is Correlated with Tumor Invasion of Human Colorectal CancerScientific ReportsDecember 1, 2017Shu-Qin Li et al.One microgram of the RNA was used for reverse transcription using the Reverse Transcription System A3500 kit (Promega, Madison, WI). The complementary DNA (cDNA) was subsequently subjected to real-time PCR

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