Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(321) Revealing potential long non-coding RNA biomarkers in lung adenocarcinoma using long non-coding RNA-mediated competitive endogenous RNA networkBrazilian Journal of Medical and Biological ResearchAugust 7, 2017T-G. Zhu, X. Xiao, Q. Wei, M. Yue, L-X. Zhangrelying on the manufacturer's protocol. Reverse transcription reaction was implemented by means of A3500 reverse transcription system kit (Promega, USA) according to the manufacturer's protocol. We used the

(322) Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinomaOncology lettersAugust 1, 2017Yamamoto M, Yamasaki M, Tsukao Y, Tanaka K, Miyazaki Y, Makino T, Takahashi T, Kurokawa Y, Nakajima K, Takiguchi S, et al.manufacturer's protocol. Relevant complementary DNA was amplified by PCR with the Reverse Transcription System A3500 (Promega Corporation, Madison, WI, USA). Reverse transcription was performed at 42°C for 60 min followed

(323) Overexpression of long non-coding RNA H19 is associated with unfavorable prognosis in patients with colorectal cancer and increased proliferation and migration in colon cancer cellsOncology LettersAugust 1, 2017Shan-Wen Chen, Jing Zhu, Ju Ma, Jun-Ling Zhang, Shuai Zuo, Guo-Wei Chen, Xin Wang, Yi-Sheng Pan, Yu-Cun Liu, Peng-Yuan WangWaltham, MA, USA) and reverse‑transcribed into cDNA using a Reverse Transcription System kit (cat. no. A3500; Promega Corporation, Madison, WI, USA), according to the manufacturer's protocol. qPCR analysis was

(324) Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKBOncology LettersAugust 1, 2017Qiuye Ma, Yidong Yu, Linlin Dai, Xuehua Qu, Shan Cong, Hongsuo LiangTotal RNA (1 µg) was subjected to reverse transcription using a reverse transcription system (cat. no. A3500; Promega Corporation, Madison, WI, USA). qPCR was performed using SYBR‑Green PCR Master Mix (Promega

(325) Targeting MCL-1 sensitizes human esophageal squamous cell carcinoma cells to cisplatin-induced apoptosisBMC cancerJune 28, 2017Yu X, Li W, Xia Z, Xie L, Ma X, Liang Q, Liu L, Wang J, Zhou X, Yang Y, et al.First-strand cDNA was synthesized from 2 μg of total RNA using the Reverse Transcription System Kit (Cat. No. A3500, Promega, Madison, WI). The resulted cDNA was subjected to PCR (95 °C for 5 min followed by 36 cycles

(326) Effects of AQP5 gene silencing on proliferation, migration and apoptosis of human glioma cells through regulating EGFR/ERK/ p38 MAPK signaling pathwayOncotargetJune 13, 2017Jian Yang et al.42°C for 60 min, 95°C for 10 min, −40°C after termination. qRT-PCR was conducted using the PCR kit (A3500, Promega Corp., Madison, Wisconsin, USA) and the total volume of PCR reaction included 20 μL: 1 μL cDNA,

(327) Modeling Psychomotor Retardation using iPSCs from MCT8-Deficient Patients Indicates a Prominent Role for the Blood-Brain BarrierCell Stem CellJune 1, 2017Gad D. Vatine et al.Promega). Complementary DNA was generated from 0.5 mg total RNA using a Reverse Transcript System (Promega A3500). Primer sequences are included in Table S6. qPCR was performed using a 384-well format (BioRad). GAPDH

(328) Expression and immunogenicity of NY-ESO-1 in colorectal cancerExperimental and therapeutic medicineJune 1, 2017Li Y, Song R, Li X, Xu F.Fisher Scientific, Inc.). Total RNA was reverse transcribed by using a reverse transcription system kit (cat. no. A3500; Promega Corp., Madison, WI, USA) according to the manufacture's protocol. The PCR primers were designed

(329) Trehalose metabolism genes of Aphelenchoides besseyi (Nematoda: Aphelenchoididae) in hypertonic osmotic pressure survivalBiology OpenMay 15, 2017Qiaoli Chen, Danlei Li, Feng Wang, Ruizhi Zhang, Yaming Lingal., 2016, 2012). Then the Promega AMV reverse transcription system was used (Promega, USA, cat. no. A3500) according to the manufacturer's instructions and Oligo (dT)18 was used as a primer for the first chain

(330) A Systematic RNAi Screen Reveals a Novel Role of a Spindle Assembly Checkpoint Protein BuGZ in Synaptic Transmission in C. elegansFrontiers in Molecular NeuroscienceMay 11, 2017Mei Han et al.using Trizol reagent (Invitrogen). Total RNA was reversed to cDNA using Reverse Transcription System A3500 (Promega). For quantitative RT-PCR, Bio-Rad CFX96 real-time PCR Amplifier was used to run the cycles.

(331) Distinct behavioral and brain changes after different durations of the modified multiple platform method on rats: An animal model of central fatiguePLOS ONEMay 11, 2017Chenxia Han, Feng Li, Jie Ma, Yan Liu, Weihong Li, Yingqiu Mao, Yuehan Song, Siyuan Guo, Jing Liutotal RNA (1μg) was reverse-transcribed to 50μl cDNA using a Reverse Transcription System (Promega A3500). Both methods were performed according to the manufacturer’s instructions. Primers were designed using

(332) Genome-wide analysis of long non-coding RNA expression and function in colorectal cancerTumor BiologyMay 5, 2017Fangyuan Jing, Huicheng Jin, Yingying Mao, Yingjun Li, Ye Ding, Chunhong Fan, Kun Chenvalidation. Total RNA (0.5 µg) was used as a template to prepare complementary DNA (cDNA; cat. no. A3500; Reverse Transcription System; Promega Corporation, Madison, WI, USA). The lncRNA expression level was quantified

(333) Effect of mild hypothermia preconditioning against low temperature (4°C) induced rat liver cell injury in vitroPLOS ONEApril 28, 2017Jiasheng Qin, Yanxing Mai, Yang Li, Zesheng Jiang, Yi Gaothe manufacturer’s instructions, followed by converting to cDNA using Reverse Transcription System (A3500, Promega). Then, the cDNA samples were used for Real-Time Quantitative PCR (qPCR) with SYBR Green qPCR

(334) Bone marrow-derived mesenchymal stem cells overexpressing miR-21 efficiently repair myocardial damage in ratsOncotargetApril 25, 2017Yan-Ling Zeng et al.reverse transcription of total RNA (30 μg) was conducted according to Reverse Transcription System A3500 kit (Promega, USA) followed by PCR on the ABI 7500 (Applied Biosystem, CA, USA). The PCR conditions

(335) Overexpression of an EaZIP gene devoid of transit peptide sequence induced leaf variegation in tobaccoPLOS ONEApril 19, 2017Xiayu Guan, Zhijian Li, Zhiliang Zhang, Xiangying Wei, Jiahua Xie, Jianjun Chen, Qingxi Chengene-containing plasmid. For detection of EaZIP transcript in transgenic tobacco, a reverse transcription system (A3500) from Promega Co. (Madison, WI, USA) was used. Following the reverse transcription reaction with total

(336) An Exploration of Traditional Chinese Medicinal Plants with Anti-Inflammatory ActivitiesEvidence-Based Complementary and Alternative MedicineApril 4, 2017Chao Fan, Hui-zi Jin, Lehao Wu, Yu Zhang, Richard D. Ye, Weidong Zhang, Yan ZhangCarlsbad, CA, USA). Reverse transcription of RNA was performed with the Reverse Transcription System A3500 kit (Promega, Madison, WI) according to the manufacturer's protocol. Relative quantification of gene expression

(337) M2 polarization of macrophages facilitates arsenic-induced cell transformation of lung epithelial cellsOncotargetMarch 28, 2017Cui J, Xu W, Chen J, Li H, Dai L, Frank JA, Peng S, Wang S, Chen G.(Invitrogen, 15596-026) and was reverse transcribed to cDNA using a Reverse Transcription System (Promega, A3500) according to the manufacturer's instructions. PCR was performed on a Lightcycler 480 system (Roche)

(338) HDAC1-3 inhibitor MS-275 enhances IL10 expression in RAW264.7 macrophages and reduces cigarette smoke-induced airway inflammation in miceScientific reportsMarch 27, 2017Leus NG, van den Bosch T, van der Wouden PE, Krist K, Ourailidou ME, Eleftheriadis N, Kistemaker LE, Bos S, Gjaltema RA, Mekonnen SA, et al.USA). For gene expression analysis, RNA was reverse transcribed using the Reverse Transcription kit (#A3500, Promega). Subsequently, 10 ng of cDNA was applied for each RT-qPCR, which was performed on an ABI Prism

(339) Single-Molecule Counting of Point Mutations by Transient DNA BindingScientific ReportsMarch 6, 2017Xin Su, Lidan Li, Shanshan Wang, Dandan Hao, Lei Wang, Changyuan YuPicoPure® RNA Isolation Kit was obtained from Thermo Fisher (Fremont, CA). The reverse transcription system (A3500) was from Promega (Fitchburg, WI). Taq DNA polymerase was purchased from NEB (Beverly, MA). HEK-293,

(340) Identification of Ftr1 and Zrt1 as iron and zinc micronutrient transceptors for activation of the PKA pathway in Saccharomyces cerevisiaeMicrobial CellMarch 6, 2017Joep Schothorst, Griet V. Van Zeebroeck, Johan M. TheveleinDNAse (Roche). cDNA was prepared following the instructions of the Reverse Transcription Kit by Promega A3500. Subsequently, relative quantification of FTR1/ZRT1 expression and the reference genes 18S and ACT1

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