Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(341) Maternal Low-Protein Diet Modulates Glucose Metabolism and Hepatic MicroRNAs Expression in the Early Life of Offspring †NutrientsFebruary 27, 2017Jia Zheng, Xinhua Xiao, Qian Zhang, Tong Wang, Miao Yu, Jianping XuRNA was reverse transcribed by 1 μg of total RNA from each sample using the Power cDNA Synthesis kit (A3500, Promega BioSciences LLC, Sunnyvale, CA, USA). We used Oligo 7.0 software (Molecular Biology Insights,

(342) Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulationWorld journal of gastroenterologyFebruary 14, 2017Bu LJ, Yu HQ, Fan LL, Li XQ, Wang F, Liu JT, Zhong F, Zhang CJ, Wei W, Wang H, et al.annexin V-FITC kit was obtained from Shanghai Bestbio (Shanghai, China). The reverse transcription kit (A3500) and TRIzol (15596-026) were obtained from Promega Inc. (Madison, WI, United States). The SYBR Green

(343) Prenatal alcohol exposure impairs autophagy in neonatal brain cortical microvesselsCell death & diseaseFebruary 9, 2017Girault V, Gilard V, Marguet F, Lesueur C, Hauchecorne M, Ramdani Y, Laquerrière A, Marret S, Jégou S, Gonzalez BJ, et al.generated cDNA by the reverse transcription of 0.5 μg of total RNA in a final volume of 20 μl with the A3500 reverse transcription system (Promega, Charbonnieres Les Bains, France). Quantitative PCR was then performed

(344) Efficient biosynthesis of a Cecropin A-melittin mutant in Bacillus subtilis WB700Scientific ReportsFebruary 6, 2017Shengyue Ji, Weili Li, Abdul Rasheed Baloch, Meng Wang, Hengxin Li, Binyun Cao, Hongfu ZhangThe extracted total RNA was reverse-transcribed into cDNA using a reverse transcription system kit (#A3500; Promega, USA). Real-time PCR was subsequently performed using a real-time PCR kit (#DRR041S; TaKaRa,

(345) Stattic and metformin inhibit brain tumor initiating cells by reducing STAT3-phosphorylationOncotargetJanuary 31, 2017Verena Leidgens et al.instructions. Reverse transcription was performed with the Reverse Transcription System (Promega, #A3500) according to the manufacturer’s protocol. Quantification of STAT3 (forward: 5’-AAA GCA GCA AAG AAG

(346) Morphological Plasticity of Emerging Purkinje Cells in Response to Exogenous VEGFFrontiers in Molecular NeuroscienceJanuary 30, 2017Leonard Herrfurth, Verena Theis, Veronika Matschke, Caroline May, Katrin Marcus, Carsten Theisswater and frozen at −80°C for storage. cDNA synthesis was performed using Reverse Transcription System (A3500, Promega) according to manufacturer’s protocol using 1 μg total RNA and Oligo(dT)15 Primers. Reaction

(347) Relationship analysis between gene expression profiles and rat liver cirrhosis occurrenceAfrican Journal of BiotechnologyJanuary 25, 2017Wang Gaiping, Zhao Congcong, Chen Meng, Chen Shasha, Chang Cuifang, Xu Cunshuaneach of sample was reverse-transcribed using random primers and reverse transcription kit (Promega, A3500, USA). First-strand Wang et al. 149 Figure 1. Histopathological changes of liver tissues obtained

(348) LHBs can elevate the expression of MDR1 through HIF-1α in patients with CHB infection: a comparative proteomic studyOncotargetJanuary 17, 2017Shiying Li et al.manufacturer's instructions. First-strand cDNA was reverse-transcribed from 2 μg of total RNA using an A3500 Reverse Transcription System (Promega, Madison, WI). RT-qPCR was performed on an ABI 7300 system (Applied Biosystems

(349) The expression and function of epithelial membrane protein 1 in laryngeal carcinomaInternational Journal of OncologyJanuary 1, 2017Hong Li, Xiaowen Zhang, Xuejun Jiang, Xu JiReverse transcription into cDNA was then performed on total RNA, using the Reverse Transcription system (A3500; Promega, Madison, WI, USA) per the manufacturer's instructions. Real-time PCR was performed using

(350) Effect of Circular ANRIL on the Inflammatory Response of Vascular Endothelial Cells in a Rat Model of Coronary AtherosclerosisCellular Physiology and BiochemistryJanuary 1, 2017Chun-Li Song et al.RNA was subjected to reverse transcription, which was performed according to the instructions of an A3500 reverse transcription kit (Promega Corp., Madison, Wisconsin, USA). The PCR primers (Table 1) were designed

(351) Responses of Phospholipase D and Antioxidant System to Mechanical Wounding in Postharvest Banana FruitsJournal of Food QualityJanuary 1, 2017Li Li et al.prior to RTPCR. Total RNA (5 𝜇g) was used for the cDNA synthesis with the Reverse Transcription System A3500 (Promega, Madison, WI, USA) according to the protocol provided by the manufacturer. The fragment of

(352) β-Catenin Directs Nuclear Factor-κB p65 Output via CREB-Binding Protein/p300 in Human Airway Smooth MuscleFrontiers in immunologyJanuary 1, 2017Koopmans T, Eilers R, Menzen M, Halayko A, Gosens R.instructions. cDNA levels were normalized and synthesized using AMV reverse transcriptase (Promega, #A3500), and thereafter diluted 15 times with RNAse-free ddH2O. Quantitative real-time PCR was conducted with

(353) Effects of PKM2 Gene Silencing on the Proliferation and Apoptosis of Colorectal Cancer LS-147T and SW620 CellsCellular Physiology and BiochemistryJanuary 1, 2017Ran Ao, Lin Guan, Ying Wang, Jia-Ni Wang1). Reverse transcription was conducted following the instructions of Reverse Transcription System A3500 (Promega Corporation, Madison, WI, USA). Reaction conditions were as follows: initial denaturation at

(354) Differentially expressed proteins underlying childhood cortical dysplasia with epilepsy identified by iTRAQ proteomic profilingPloS oneJanuary 1, 2017Qin L, Liu X, Liu S, Liu Y, Yang Y, Yang H, Chen Y, Chen L.extracted using Trizol (Thermofisher, Waltham, USA). Extracted RNA was reverse transcribed into cDNA by A3500 Reverse Transcription System (Promega, Madison, PLOS ONE | DOI:10.1371/journal.pone.0172214 February

(355) Up-Regulation of HMGB1 Exacerbates Renal Ischemia-Reperfusion Injury by Stimulating Inflammatory and Immune Responses through the TLR4 Signaling Pathway in MiceCellular Physiology and BiochemistryJanuary 1, 2017Chuan-Bao Chen, Long-Shan Liu, Jian Zhou, Xiao-Ping Wang, Ming Han, Xing-Yuan Jiao, Xiao-Shun He, Xiao-Peng Yuanof RNA was reverse transcribed in accordance with the specification of Reverse Transcription System A3500 (Promega Biotech Co., Ltd., Madison, Wisconsin, USA). According to the gene sequences published by Genbank,

(356) 4'-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury micePloS oneJanuary 1, 2017Fan C, Wu LH, Zhang GF, Xu F, Zhang S, Zhang X, Sun L, Yu Y, Zhang Y, Ye RD.Carlsbad, CA, USA). Reverse transcription of RNA was performed with the Reverse Transcription System A3500 kit (Promega, Madison, WI) according to the manufacturer’s protocol. Relative quantification of gene expression

(357) Effect of TNF-α Inhibition on Bone Marrow-Derived Mesenchymal Stem Cells in Neurological Function Recovery after Spinal Cord Injury via the Wnt Signaling Pathway in a Rat ModelCellular Physiology and BiochemistryJanuary 1, 2017Ren-Jun Peng, Bing Jiang, Xi-Ping Ding, He Huang, Yi-Wei Liao, Gang Peng, Quan Cheng, Jian XiCalifornia, USA). PCR was performed according to the procedures of the Reverse Transcription System A3500 (Promega Corp., Madison, Wisconsin, USA). The reaction conditions were: initial denaturation for 15

(358) Overexpression of ATPase Na+/K+ transporting alpha 1 polypeptide, ATP1A1, correlates with clinical diagnosis and progression of esophageal squamous cell carcinomaOncotargetDecember 20, 2016I-Chen Wu et al.Inc.). All of the reverse transcription reactions were performed by Reverse Transcription System (Cat. no: A3500, Promega, Madison, WI, USA). In order to achieve optimal reverse transcription efficiency, 1 μg total

(359) YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells: new insights about YWHAE role in the tumor development and metastasis processOncotargetDecember 20, 2016Leal MF, Ribeiro HF, Rey JA, Pinto GR, Smith MC, Moreira-Nunes CA, Assumpção PP, Lamarão LM, Calcagno DQ, Montenegro RC, et al.reverse transcribed using the Reverse Transcription System according to the manufacturer's protocol (A3500; Promega, USA). Complementary DNA was then amplified by real-time reverse transcription quantitative PCR

(360) Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodelingBMC GenomicsDecember 1, 2016Genny Degani et al.synthesized from 1.6 μg of total RNA in 20 μl final volume, using the Reverse Transcription System A3500 (Promega). As a test for residual genomic DNA contamination, reactions were performed in the absence

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