Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(221) The RNA hairpin binder TRIM71 modulates alternative splicing by repressing MBNL1Genes & DevelopmentSeptember 1, 2019Thomas Welte, Alex C. Tuck, Panagiotis Papasaikas, Sarah H. Carl, Matyas Flemr, Philip Knuckles, Aneliya Rankova, Marc Bühler, Helge GroßhansFour-hundred nanograms of total RNA was transcribed to cDNA using the reverse transcription system (Promega, A3500) and random primers. qPCRs were performed using the SYBR Green PCR master mix (Applied Biosystems, 4309155)

(222) Oncostatin M receptor, positively regulated by SP1, promotes gastric cancer growth and metastasis upon treatment with Oncostatin MGastric CancerSeptember 1, 2019Zhenjia Yu et al.

(223) Investigation into the expression levels of MAGEA6 in esophageal squamous cell carcinoma and esophageal adenocarcinoma tissuesExperimental and therapeutic medicineSeptember 1, 2019Hao J, Li S, Li J, Jiang Z, Ghaffar M, Wang M, Jia R, Chen S, Wang Y, Zeng Y.cDNA of all the cell lines was then synthesized using the Reverse Transcription System MK (cat. no. A3500; Promega Corporation, Madison, WI, USA). The temperature protocol was as follows: 42˚C for 1 h, 95˚C

(224) Inhibitory selectivity among class I HDACs has a major impact on inflammatory gene expression in macrophagesEuropean Journal of Medicinal ChemistrySeptember 1, 2019Fangyuan Cao, Martijn R.H. Zwinderman, Ronald van Merkerk, Petra E. Ettema, Wim J. Quax, Frank J. DekkerWilmington, DE, USA). Then, RNA was reverse transcribed to cDNA using the Reverse Transcription Kit SC (#A3500, Promega). 10 ng of cDNA, 5 µL 2x SensiMix SYBR Lo-ROX and 0.4 µL primers were applied for each RT-qPCR,

(225) MicroRNA-101a Regulates Autophagy Phenomenon via the MAPK Pathway to Modulate Alzheimer's-Associated PathogenesisCell transplantationAugust 1, 2019Li Q, Wang Y, Peng W, Jia Y, Tang J, Li W, Zhang JH, Yang J.from the ‘screening’ sets via reverse transcription with the Reverse Transcription System (Promega, A3500, Madison, WI, USA), which consists of stem-looped reverse transcription primers. By using the LightCycler

(226) Inhibitor of DNA binding 1 (Id1) mediates stemness of colorectal cancer cells through the Id1-c-Myc-PLAC8 axis via the Wnt/β-catenin and Shh signaling pathwaysCancer Management and ResearchJuly 23, 2019Yanxia Sun et al.Valencia, CA, USA) and reverse-transcribed into cDNA using the Promega Reverse Transcription System A3500 (Promega; Madison, WI, USA). qPCR assay was run on a LightCycler Roche 480 Real-time PCR system (Roche

(227) Inhibitor of DNA binding 1 (Id1) mediates stemness of colorectal cancer cells through the Id1-c-Myc-PLAC8 axis via the Wnt/β-catenin and Shh signaling pathwaysCancer Manag ResJuly 23, 2019Yanxia Sun, Xiaolan Lai, Yue Yu, Jieyu Li, Lei Cao, Wansong Lin, Chuanzhong Huang, Jinrong Liao, Wei Chen, Chao Li, et al.Valencia, CA, USA) and reverse-transcribed into cDNA using the Promega Reverse Transcription System A3500 (Promega; Madison, WI, USA). qPCR assay was run on a LightCycler Roche 480 Real-time PCR system (Roche

(228) Effects of Hexane Root Extract ofFerula hermonisBoiss. on Human Breast and Colon Cancer Cells: AnIn VitroandIn VivoStudyBioMed Research InternationalJuly 15, 2019Nael Abutaha, Fahd A. Nasr, Mohammed Al-zharani, Ali S. Alqahtani, Omar M. Noman, Mohammed Mubarak, Semlali Abdelhabib, Muhammad A. WadaancDNA was synthesized from 1 𝜇g of RNA using Promega kit (Reverse Transcription System (100), Cat. No. A3500, USA). q-PCR was carried out on BioMed Research International 3 an Applied Biosystems 7500 Fast Real-Time PCR System

(229) Thermal pretreatment promotes the protective effect of HSP70 against tendon adhesion in tendon healing by increasing HSP70 expressionMolecular medicine reportsJuly 1, 2019Tang XM, Dai J, Sun HL.Spectrophotometer (Thermo Fisher Scientific, Inc.). Then, HSP70 mRNA was reversely transcribed using the A3500 RT system (Promega Corporation, Madison, WI, USA) at 42°C for 1 h and at 95°C for 5 min according to

(230) Berberine improves advanced glycation end products‑induced osteogenic differentiation responses in human periodontal ligament stem cells through the canonical Wnt/β‑catenin pathwayMolecular medicine reportsJune 1, 2019Zhang LN, Wang XX, Wang Z, Li KY, Xu BH, Zhang J.transcribed into complementary DNA (cDNA) using Promega Reverse Transcription System (cat. no. Promega A3500; Promega Corporation) with the following parameters: 60 min at 42°C, 5 min at 95°C, and 3 min at 4°C.

(231) Nutrient availability shapes methionine metabolism in p16/MTAP-deleted cellsScience advancesJune 1, 2019Sanderson SM, Mikhael PG, Ramesh V, Dai Z, Locasale JW.RNA was then converted to single-strand complementary DNA using the Reverse Transcription System (no. A3500, Promega). Expression levels of Cdkn2a, MTAP, and 18-S transcripts were assessed by qPCR on a CFX384 Touch Real-Time PCR Detection System

(232) Dexamethasone-Induced Mitochondrial Dysfunction and Insulin Resistance-Study in 3T3-L1 Adipocytes and Mitochondria Isolated from Mouse LiverMoleculesMay 23, 2019Guangxiang Luan, Gang Li, Xiao Ma, Youcai Jin, Na Hu, Ji Li, Zhenhua Wang, Honglun WangReverse transcription was performed with 1 µg total RNA and M-MLV Reverse Transcriptase Kit (Promega A3500; Promega, Madison, WI, USA). Briefly, 40 µL of total reaction volume was used in a Veriti 96 Well Thermal

(233) Impeding the single-strand annealing pathway of DNA double-strand break repair by withaferin A-mediated FANCA degradationDNA repairMay 1, 2019Liu W, Wang G, Palovcak A, Li Y, Hao S, Liu ZJ, Landgraf R, Yuan F, Zhang Y.on an agarose gel. cDNA synthesis was carried out by using reverse transcription kit from Promega (A3500) following manufacturer’s protocol. Sybr green (applied Biosystems cybrgreen 4367659) mixes of cDNA

(234) UBTD1 is a mechano-regulator controlling cancer aggressivenessEMBO reportsApril 1, 2019Torrino S, Roustan FR, Kaminski L, Bertero T, Pisano S, Ambrosetti D, Dufies M, Uhler JP, Lemichez E, Mettouchi A, et al.Spectrophotometer (Thermo Scientific). One microgram of total RNA was reverse-transcribed into cDNA (A3500, Promega). Real-time quantitative PCR was performed using Fast SYBR Green Master Mix (Applied Biosystems) on

(235) Spinal macrophage migration inhibitory factor and high mobility group box 1 mediate persistent bladder painNeuroscience LettersApril 1, 2019Fei Ma, Katherine L. Meyer-Siegler, Lin Leng, Richard Bucala, Pedro L. VeraThermoFisher Scientific, Grand Island, NY), DNA removed by DNase, and reversed transcribed to cDNA (A3500, Promega, Madison, WI). PCR products were detected using SYBR green (4472903, ThermoFisher Scientific,

(236) Nonviral ultrasound-mediated gene delivery in small and large animal modelsNature ProtocolsApril 1, 2019Maxim Bez, Josquin Foiret, Galina Shapiro, Gadi Pelled, Katherine W. Ferrara, Dan GazitC0130)Medical-grade oxygenRNeasy isolation kit (Qiagen, cat. no. 74104)Reverse transcription kit (Promega, cat. no. A3500) Equipment VialMix shaker (Lantheus Medical, cat. no. VMIX)Siemens Antares system with a colinear

(237) TGFβ1 and HGF regulate CTGF expression in human atrial fibroblasts and are involved in atrial remodelling in patients with rheumatic heart diseaseJournal of cellular and molecular medicineApril 1, 2019Chen JQ, Guo YS, Chen Q, Cheng XL, Xiang GJ, Chen MY, Wu HL, Huang QL, Zhu PL, Zhang JC.Beijing, China), a Gene Amp 9700 PCR amplification instrument (ThermoFisher, Waltham, MA, USA), an RT‐PCR A3500 kit (Promega), a NanoDrop ND‐1000 UV spectrophotometer (NanoDrop, Wilmington, DE, USA), an ImageMaster

(238) Ginsenoside Rb2 Alleviates Obesity by Activation of Brown Fat and Induction of Browning of White FatFront Endocrinol (Lausanne)March 15, 2019Yilian Hong, Yi Lin, Qiya Si, Lijuan Yang, Weisong Dong, Xuejiang Gu(Invitrogen, Carlsbad, CA) and was reverse transcribed to first-strand cDNA using the Reverse Transcription System (A3500, Promega, Madison, WI). To analyze gene expression, real-time quantitative PCR was conducted by using

(239) In vitro Technical Aspects of Anti-Gene IGF-I Vaccines against GliomaInsights of Biomedical ResearchJanuary 16, 2019Trojan Jerzy et al.GmbH nr 1828665), and RT PCR was used according Reverse Transcription System Promega Corporation (nr A3500). The following primers were used for RT PCR study of rat IGF-I and rat MHC-I: forward primer IGF-I:

(240) Atorvastatin improves left ventricular remodeling and cardiac function in rats with congestive heart failure by inhibiting RhoA/Rho kinase-mediated endothelial nitric oxide synthaseExperimental and therapeutic medicineJanuary 1, 2019An L, An S, Jia Z, Wang H, Yang Z, Xu C, Teng X, Wang J, Liu X, Cao Q, et al.isothiocyanate‑phenol‑chloroform method (25) and RT‑PCR was performed according to the kit instructions (A3500 reverse transcription kit; Promega Corporation, Madison, WI, USA). The reaction mixture comprised primers,

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