Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(241) The level of intracellular tacrolimus in T cell is affected by CD44⁺ABCB1⁺activities triggered by inflammationEuropean Journal of InflammationJanuary 1, 2019Eunjin Bae et al.Germany), and RNA was converted to cDNA using a kit according to the manufacturer’s instructions (cat. no. A3500; Promega Corporation, Madison, WI, USA). PCRs were carried out on an Applied Biosystem™ 7500 thermocycler

(242) Development of wheat genotypes expressing a glutamine-specific endoprotease from barley and a prolyl endopeptidase from Flavobacterium meningosepticum or Pyrococcus furiosus as a potential remedy to celiac diseaseFunctional & Integrative GenomicsJanuary 1, 2019Claudia E. Osorio, Nuan Wen, Jaime H. Mejias, Bao Liu, Stephen Reinbothe, Diter von Wettstein, Sachin Rustgi(Bio-Rad), and converted to cDNA with oligo-dT primer using the Reverse Transcription System (catalog no. A3500; Promega) following the manufacturer’s instructions. The qRT-PCR analysis was performed on the LightCycler

(243) Transgenic expression of cyclooxygenase-2 in pancreatic acinar cells induces chronic pancreatitisAmerican Journal of Physiology-Gastrointestinal and Liver PhysiologyJanuary 1, 2019Haojie Huang et al.transcription was performed with 1 ␮g of purified total RNA in a 25-␮l volume of RT reaction mixture (A3500, Promega, Carlsbad, CA). Quantitative PCR was conducted using SYBR green I to monitor the PCR products

(244) P-TEFb Regulates Transcriptional Activation in Non-coding RNA GenesFrontiers in geneticsJanuary 1, 2019Bunch H, Choe H, Kim J, Jo DS, Jeon S, Lee S, Cho DH, Kang K.converted to cDNA by reverse transcription using a Promega Reverse Transcription System (Promega, Cat. A3500, United States) or Toyobo ReverTra Ace qPCR RT Master Mix (Toyobo, Japan), according to the manufacturers’

(245) miR167 limits anther growth to potentiate anther dehiscenceDevelopmentJanuary 1, 2019Lanjie Zheng, Punita Nagpal, Gonzalo Villarino, Brendan Trinidad, Laurina Bird, Yubi Huang, Jason W. Reedextracted using Trizol (Invitrogen) as described by the manufacturer. cDNA was synthesized using the A3500 Reverse Transcription System (Promega) according to the manufacturer’s instructions. For the mRNA, reverse

(246) A Five-microRNA Signature for Survival Prognosis in Pancreatic Adenocarcinoma based on TCGA DataScientific ReportsDecember 1, 2018Xiu-Hui Shi et al.spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription reactions using the A3500 reverse transcription system kit (Promega, Madison, WI, USA) were conducted in two steps according to

(247) Social rank-associated stress vulnerability predisposes individuals to cocaine attractionScientific ReportsDecember 1, 2018Chen Yanovich, Michael L. Kirby, Izhak Michaelevski, Gal Yadid, Albert Pinhasovkit (5 Prime, USA, Cat. 2302410) and reverse transcribed to cDNAs with random primers (Promega, USA, A3500). Levels of mRNA were analyzed using a SYBR FAST Universal Readymix Kit (KAPA Biosystems, Wilmington,

(248) Role of MCP-1 and CCR2 in ethanol-induced neuroinflammation and neurodegeneration in the developing brainJournal of NeuroinflammationDecember 1, 2018Kai Zhang, Haiping Wang, Mei Xu, Jacqueline A. Frank, Jia Luothe manufacturer’s instructions. 1 μg of total RNA was used for first strand cDNA synthesis (Promega, A3500). Quantitative real-time RT-PCR was performed on a Lightcycler 480 system (Roche) using a Power SYBR

(249) Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3Molecular and Cellular ProbesDecember 1, 2018Ning Sun, Weiping Wang, Jie Wang, Xinyue Yao, Fangfang Chen, Xiaojun Li, Yi Yinglei, Bo Chenmanufacturer. The complementary DNA (cDNA) was synthesized using the Reverse Transcription System (A3500, Promega, Madison, WI, USA). Briefly, in a 0.2 ml tube, 5 μl of RNA, 4 μl of MgCl2 (25 mM), 2 μl of

(250) Retinal S-opsin dominance in Ansell’s mole-rats (Fukomys anselli) is a consequence of naturally low serum thyroxineScientific ReportsDecember 1, 2018Yoshiyuki Henning, Nella Mladěnková, Hynek Burda, Karol Szafranski, Sabine BegallGermany). RNA was reverse-transcribed into complementary DNA (cDNA) using AMV Reverse Transcription System (A3500, Promega, Mannheim, Germany) according to manufacturer’s instructions. We further added 10 µg BSA per

(251) Influence of silver nanoparticles on growth and health of broiler chickens after infection with Campylobacter jejuniBMC Veterinary ResearchDecember 1, 2018Krishna Prasad Vadalasetty, Charlotte Lauridsen, Ricarda Margarete Engberg, Radhika Vadalasetty, Marta Kutwin, André Chwalibog, Ewa Sawoszmg of total RNA was reverse-transcribed using the cDNA Reverse Transcription Kit (Promega, cat. no. A3500) in a G-storm PCR System. After this, real-time PCR was performed with complementary DNA and genespecific

(252) MicroRNA-365 alleviates morphine analgesic tolerance via the inactivation of the ERK/CREB signaling pathway by negatively targeting β-arrestin2Journal of Biomedical ScienceDecember 1, 2018Xian-Ping Wu, Rui-Xuan She, Yan-Ping Yang, Zu-Min Xing, Han-Wen Chen, Yi-Wen Zhangexpressions of β-arrestin2, ERK and CREB were detected in accordance with the Reverse Transcription System (A3500, Promega Corp., Madison, Wisconsin, USA). According to the gene sequences published in GenBank database,

(253) Food restriction decreases BMR, body and organ mass, and cellular energetics, in the Chinese Bulbul (Pycnonotus sinensis)Avian ResearchDecember 1, 2018Yiying Zhang, Ke Yang, Peipei Yang, Yingshi Su, Weihong Zheng, Jinsong LiuMicro-Ultraviolet Spectrophotometer (NanoDrop 2000c, Thermo Scientific). A Reverse Transcription System (A3500, Promega) was used to manufacture cDNA samples with total RNA as the template. A 10 μL RT mixture of

(254) The Antitumor and Immunomodulatory Effect of Yanghe Decoction in Breast Cancer Is Related to the Modulation of the JAK/STAT Signaling PathwayEvidence-Based Complementary and Alternative MedicineNovember 18, 2018Dan Mao, Lei Feng, Hui Gongextracted from isolated MDSCs and reverse transcribed to cDNA using a Power SYBR Green Cells-to-CT kit (A3500; Promega, USA). Quantitative PCR was applied using a SYBR green supermix (730003407; Bio-Rad, USA).

(255) TXNDC9 promotes hepatocellular carcinoma progression by positive regulation of MYC-mediated transcriptional networkCell Death & DiseaseNovember 1, 2018Dawei Chen, Jixue Zou, Zhenguo Zhao, Xiaodong Tang, Zhicheng Deng, Jingchao Jia, Shuanghai Liuusing an All-Prep DNA/RNA Mini kit (Qiagen, Hilden, Germany). cDNA was reverse transcribed with the A3500 RT-PCR System (Promega Corporation, Madison, WI, USA) from 1 μg of total RNA. The relative TXNDC9 mRNA

(256) miR-186 reverses cisplatin resistance and inhibits the�formation of the glioblastoma-initiating cell�phenotype by degrading Yin Yang 1 in glioblastomaInternational Journal of Molecular MedicineOctober 18, 2018Jian Li, Jie Song, Feng Guo(RT) system followed by PCR amplification in a 50 µl PCR system performed using an RT‑PCR kit (Cat no. A3500, Promega, Madison, WI, USA). The housekeeping gene, glyceraldehyde‑3‑phosphate dehydrogenase (GAPDH),

(257) Anti-Inflammatory Activities of Compounds Isolated from the Rhizome of Anemarrhena asphodeloidesMolecules (Basel, Switzerland)October 13, 2018Wang Z, Cai J, Fu Q, Cheng L, Wu L, Zhang W, Zhang Y, Jin Y, Zhang C.Carlsbad, CA, USA). Reverse transcription of RNA was performed with the Reverse Transcription System A3500 kit (Promega, Madison, WI, USA) according to the manufacturer0 s protocol. The complementary DNA (cDNA)

(258) Plasma interleukin-37 is increased and inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells in systemic juvenile idiopathic arthritis patientsJournal of translational medicineOctober 11, 2018Feng M, Kang M, He F, Xiao Z, Liu Z, Yao H, Wu J.(Invitrogen, USA), according to the manufacturer’s instructions. cDNAs were obtained using the RT System A3500 Kit (Promega, USA). The primer sequences were summarized in Table 2. RT-PCR amplification reactions

(259) Protease activated-receptor 4 activation as a model of persistent bladder pain: Essential role of macrophage migration inhibitory factor and high mobility group box 1International Journal of UrologyOctober 1, 2018Fei Ma, David E Hunt, Lin Leng, Richard Bucala, Katherine L Meyer-Siegler, Pedro L VeraThermo-Fisher Scientific, Grand Island, NY, USA), DNA removed by DNase and reversed transcribed to cDNA (A3500; Promega, Madison, WI, USA). PCR products were detected using SYBR green (4472903; ThermoFisher Scientific)

(260) Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiationEBioMedicineOctober 1, 2018Ng-Blichfeldt JP, Schrik A, Kortekaas RK, Noordhoek JA, Heijink IH, Hiemstra PS, Stolk J, Königshoff M, Gosens R.NanoDrop ND-1000 spectrophotometer. cDNA was synthesized using AMV reverse transcriptase (Promega, #A3500), and diluted with ddH2O proportionately according to concentration of input RNA. qRT-PCR was performed

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