Reverse Transcription System from Promega

Product Specs
ItemReverse Transcription System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3500
Quantity100 reactions
PrimersOligo(dT)₁₅ Primer and Random Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(261) Changes in thyroid status of Menidia beryllina exposed to the antifouling booster irgarol: Impacts of temperature and salinityChemosphereOctober 1, 2018Lucas Buruaem Moreira, Graciel Diamante, Marissa Giroux, Elvis Genbo Xu, Denis Moledo de Souza Abessa, Daniel Schlenk(NanoDrop®, Technologies Inc.) and the synthesis of cDNA was performed following the Quick Protocol A3500 (Promega Reverse Transcription System kit, Wisconsin, USA). Quantitative polymerase chain reaction (qPCR)

(262) Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteinsPLoS biologyOctober 1, 2018Panda S, Setia M, Kaur N, Shepal V, Arora V, Singh DK, Mondal A, Teli A, Tathode M, Gajula R, et al.internal control Gapdh at 42 °C for 90 minutes using a Reverse Transcription System kit (Promega, # A3500). Semiquantitative PCR was performed using Taq DNA polymerase (Merck Bioscience; Darmstadt, Germany).

(263) Mitochondrial Glycolysis in a Major Lineage of EukaryotesGenome Biology and EvolutionSeptember 1, 2018Carolina Río Bártulos et al.protocol (Thermo Fisher, Germany) and cDNA synthesis was performed with the reverse Transcription system (A3500, Promega, Germany). An Achlya bisexualis cDNA library (Bhattacharya et al. 1991) was kindly provided

(264) Loss of KLF15 accelerates chronic podocyte injuryInternational journal of molecular medicineSeptember 1, 2018Han SS, Yu MY, Yoo KD, Lee JP, Kim DK, Kim YS, Yang SH.Subsequently, RNA was converted to cDNA using a kit according to the manufacturer's instructions (cat. no. A3500; Promega Corporation, Madison, WI, USA). The TaqMan gene expression assay was used for human fibronectin

(265) The Octadecanoid Pathway, but Not COI1, Is Required for Nectar Secretion in Arabidopsis thalianaFrontiers in Plant ScienceAugust 8, 2018Anthony J. Schmitt, Rahul Roy, Peter M. Klinkenberg, Mengyuan Jia, Clay J. CarterRNA (500–1,000 ng) was used as template for Promega’s Reverse Transcription System (Promega, Catalog #A3500). 10 ng of the resulting cDNA was added to the real time PCR reaction mix, which included 10 μl of 2×

(266) Ultrasound‐guided intertumoral injection of contrast agents combined with human p53 gene for the treatment of breast cancerThe Kaohsiung Journal of Medical SciencesAugust 1, 2018Jun‐Cheng Guo, Yi‐Jun Yang, Min Guo, Xiang‐Ling Jiangafter which 500 ng RNA was subjected to reverse transcription using the Reverse Transcription System A3500 kit (A3500, Promega Corp., Madison, Wisconsin, USA) according to the manufacturer's instructions. According

(267) Demographic fluctuation of community-acquired antibiotic-resistant Staphylococcus aureus lineages: potential role of flimsy antibiotic exposureThe ISME JournalAugust 1, 2018Claude-Alexandre Gustave et al.by real-time PCR A random-primers based reverse transcription of 1 µg of RNA was performed with the A3500 Reverse Transcription System Kit (Promega), followed by quantitative real-time PCR on cDNA using the

(268) LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma CellsImmunityJuly 17, 2018Lino AC, Dang VD, Lampropoulou V, Welle A, Joedicke J, Pohar J, Simon Q, Thalmensi J, Baures A, Flühler V, et al.PharMingen,Cat. #554715 One Step RT-PCR kit,QIAGEN,Cat. #210212 Reverse Transcription System,Promega,Cat. #A3500 LightCycler FastStart DNA MasterPLUS SYBR Green,Roche Diagnostik,Cat. #03515885001 "Bio-Plex Pro Mouse

(269) MicroRNA‑101‑3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9Experimental and Therapeutic MedicineJuly 4, 2018Qifang Jin, Wenfeng He, Leifeng Chen, Yang Yang, Ke Shi, Zhipeng Youto reverse transcribe the mRNA into cDNA. The RT reaction was performed using an RT system (cat. no. A3500; Promega Corporation, Madison, WI, USA). The temperature protocol for RT was as follows: 42°C for 30

(270) Exosomal MALAT1 derived from oxidized low-density lipoprotein-treated endothelial cells promotes M2 macrophage polarizationMolecular medicine reportsJuly 1, 2018Huang C, Han J, Wu Y, Li S, Wang Q, Lin W, Zhu J.total RNA was used for first-strand cDNA synthesis using the reverse transcription system (cat. no. A3500; Promega Corporation, Madison, WI, USA) according to the manufacturer's protocol. The synthesized first-strand

(271) Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-HInternational journal of oncologyJuly 1, 2018Lin Q, Peng S, Yang Y.to the manufacturer's instructions. First strand cDNA was synthesized using 2 µg total RNA and the A3500 Reverse Transcription System (Promega, Madison, WI, USA). The resulting cDNA was amplified by PCR using an

(272) IGF‐1 defends against chronic‐stress induced depression in rat models of chronic unpredictable mild stress through the PI3K/Akt/FoxO3a pathwayThe Kaohsiung Journal of Medical SciencesJuly 1, 2018Wei‐Hong Kuang, Zai‐Quan Dong, Lian‐Tian Tian, Jin LiCo., Ltd. (Shanghai, China). Reverse transcription was conducted using Reverse Transcription System A3500 (Promega, Madison, WI, USA), as described by the manufacturer. cDNA was obtained to undergo RT‐qPCR

(273) Expression of a rice soluble starch synthase gene in transgenic wheat improves the grain yield under heat stress conditionsIn Vitro Cellular & Developmental Biology - PlantJune 1, 2018Bin Tian, Shyamal K. Talukder, Jianming Fu, Allan K. Fritz, Harold N. Trickmicrogram of treated total RNA was used as template using a reverse transcription system kit (Catalog no. A3500, Promega®) following the product protocol. PCR was conducted using osSSI primers described above. DNA contamination

(274) miR‑204 functions as a tumor suppressor gene, at least partly by suppressing CYP27A1 in glioblastomaOncology LettersMay 30, 2018Jun Xin, Li‑Min Zheng, De‑Ke Sun, Xian‑Feng Li, Peng Xu, Li‑Qiang Tiantranscription system followed by PCR amplification in a 50 µl PCR system performed using an RT-PCR kit (cat no. A3500; Promega Corporation). The 3′UTR of CYP27A1 was amplified [10X buffer 3 ul, MgCl2 (25 mM) 3 ul, dNTP

(275) IL‑33 notably inhibits the growth of colon cancer cellsOncology LettersMay 16, 2018Xuhui Chen et al.HCT-116 RNA was used to complete the reverse transcription with a Reverse Transcription system (Cat. no. A3500; Promega Corporation, Madison, WI, USA), as previously described (12,13,18–20). GAPDH was used as an

(276) Asparaginyl endopeptidase enhances pancreatic ductal adenocarcinoma cell invasion in an exosome-dependent manner and correlates with poor prognosisInternational journal of oncologyMay 1, 2018Yan Q, Yuan WB, Sun X, Zhang MJ, Cen F, Zhou SY, Wu WB, Xu YC, Tong LH, Ma ZH.was reverse transcribed from 1 µg total RNA using the Promega Reverse Transcription System (cat no. A3500; Promega Corporation, Madison, WI, USA). qPCR was performed with the SYBR Premix Ex Taq kit (Takara

(277) Nuclear-bound quarkonia and heavy-flavor hadronsProgress in Particle and Nuclear PhysicsMay 1, 2018G. Krein, A.W. Thomas, K. Tsushima(NanoDrop®, Technologies Inc.) and the synthesis of cDNA was performed following the Quick Protocol A3500 (Promega Reverse Transcription System kit, Wisconsin, USA). Quantitative polymerase chain reaction (qPCR)

(278) Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in RatsAsian Pacific journal of cancer prevention : APJCPApril 25, 2018Saad El-Din S, Fouad H, Rashed LA, Mahfouz S, Hussein RE.nm. The extracted RNA was reverse-transcribed into cDNA by a Reverse Transcription System Kit (Cat. no. A3500; Promega, Madison, WI, USA). The cDNA was generated from 5 μg of total RNA extracted with 1 μL (20 pmol)

(279) Effects of taxol resistance gene 1 on the cisplatin response in gastric cancerOncology LettersMarch 30, 2018Shuquan Duan, Jie Yin, Zhigang Bai, Zhongtao Zhangsamples were stored at −80°C for subsequent experiments. The Reverse Transcription system (cat. no. A3500; Promega Corporation, Madison, WI, USA) was used to generate cDNA libraries. qPCR was performed with

(280) SFTS phlebovirus promotes LC3-II accumulation and nonstructural protein of SFTS phlebovirus co-localizes with autophagy proteinsScientific reportsMarch 27, 2018Sun Y, Liu MM, Lei XY, Yu XJ.No. 52906). Extracted RNA was used for reverse transcription with Reverse Transcription System (PROMEGA, A3500). Then cDNA was used for Real time-PCR detection using the Lightcycler 480 SYBR Green (Roche, Cat. 04887352001).

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