Anti-CXCR4 antibodies are used in the immunodetection of the protein C-X-C motif chemokine receptor 4. In humans, the canonical protein has a reported length of 352 amino acid residues and a mass of 39.7 kDa. Its subcellular localization is in the lysosomes and cell membrane. Up to 2 different isoforms have been reported for this protein. A member of the G-protein coupled receptor 1 protein family, CXCR4 is a known receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation.
The CXCR4 marker can be used to identify Centroblasts and Transitional Immature B Cells.* Synonyms for this target antigen include D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, and CD184. CXCR4 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. Over 1900 citations in the literature describe the use of CXCR4 antibodies in research. Flow Cytometry is a widely used application for these antibodies. Western Blot and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4