Fig 2: A diagram of the mechanism of CXCR4-mediated T-ALL migration.

Fig 3: Knockdown expression of Src by siRNAs inhibited CXCR4-mediated T-ALL migration toward CXCL12. (A,B) siRNAs were transfected into JURKAT or CCRF-CEM cells using the methods described in the materials and methods section; 48 h after transfection, cell lysates were prepared for Western blot assay to detect the interference efficiency of siRNAs on Src. (C,D) Transfected cells were used in the transwell assay. Each experiment was repeated at least three times. Western blot bands were analyzed using Image Pro Plus 6.0, and fold changes of Src were normalized according to ACTB. ACTB was used as the loading controls. *p < 0.05, **p < 0.01, comparing with the negative control (CXCL12-) group. #p < 0.05 comparing with the positive control (CXCL12+) group.

Fig 4: Kaplan-Meier survival curves for disease-free survival (A) and overall survival (B) according to CXCR4 expression.

Fig 5: Effect of MTSS1 on the levels of the cell surface receptor, CXCR4. (A) Percentage of CXCR4-positive cells following the overexpression or knockdown of MTSS1 in SW1116 and DLD-1 cells was analyzed using flow cytometry. (B) Following 6 h of treatment with CXCL12, the percentage of CXCR4-positive cells following the overexpression or knockdown of MTSS1 was analyzed using flow cytometry. (C and D) Quantification of the data presented in the flow cytometry plots. Data are presented as the means ± SD. n=3; **P<0.01. MTSS1, metastasis suppressor 1; CXCR4, C-X-C receptor 4; CXCL12, C-X-C motif chemokine ligand 12.