Mouse CXCR4 Antibody (MAB21651, R&D systems) Binds Baf3 Cells

January 27, 2020

BlueskyReddit
Biologics
AbbVie
Research Scientist

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Company:

R&D Systems

Product Name:

Mouse CXCR4 Antibody

Catalog Number:

MAB21651

Clone Number:

247506

Flow cytometry was utilized to detect mouse CXCR4 expression on Baf3 cells. The antibody worked well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Baf3

Primary Incubation

1ug/ml, ice, 30min

Blocking Agent

BSA

Secondary Incubation

1:5000, 10 min, ice

Tertiary Incubation

N/A

Detection

APC fluorescence

Results Summary

Sub-nM binding was detected by flow cytometry for mab21651 binding to Bafs cells expressing endogenous muCXCR4.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Binding to endogenous CXCR4.

The Bad

Unknown endotoxin levels

The Bottom Line

Useful as binding reagent to CXCR4 as a control or for other assays.

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