Staining Transduced MSC With Anti-CXCR4 APC

August 09, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CXCR4 APC

Catalog Number:

306509

This antibody was used to assay for surface expression of CXCR4 in lentiviral-transduced MSC. It was chosen because it is a commonly used clone for CXCR4, APC is a bright fluorochrome, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human MSC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CXCR4 staining was excellent, with positive cells being fully resolved from negative cells and much brighter than isotype control.

Additional Notes

None

Related Categories

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Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will definitely continue using this antibody.

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