Excellent CXCR4 Staining On Human PBMCs

November 20, 2017

Infection and Immunity
University College London
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-human CD184 (CXCR4) Antibody

Catalog Number:

306509

The aim of this study was to observe CXCR4 downregulation in CD4+ T cells infected with HIV (Nef239).I chose this product as Biolegend offers good quality antibodies at good price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

1:100 (50 ul), 30min, 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Fortessa-X20

Results Summary

We can observe downregulation of CXCR4 expression on CD4+ T cells infected with HIV (Nef239).

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Very good staining at high dilution.

The Bad

None

The Bottom Line

I would recommend this product.

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