CXCR4 Antibody

Product Specs
ItemCXCR4 Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $429.00Supplier Page View Company Product Page
Catalog NumberNB100-56437
Concentration1.0 mg/ml
HostRabbit
ReactivityHuman,Mouse,Rat,Sheep
ApplicationsIF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Knockdown Validated,Immunocytochemistry/ Immunofluorescence
Quantity0.1 mg (also 0.025 mg)
Conjugate/TagUnconjugated
FormatPBS
ImmunogenRabbit anti-CXCR4 polyclonal antibody was raised against a peptide corresponding to amino acids 328-338 of human CXCR4
NCBI Full Gene NameC-X-C motif chemokine receptor 4
NCBI Gene AliasesLCR1, NPYY3R, WHIM, WHIMS1, LAP-3, HM89, LAP3, NPYRL, WHIMS, FB22, D2S201E, NPYR, NPY3R, LESTR, HSY3RR, CD184
ClonalityPolyclonal
IsotypeIgG
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
Research AreaGPCR, Metastasis
PurityProtein G purified
TargetCXCR4

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this CXCR4 antibody be used in mouse models for studying angiogenesis?

Yes, the CXCR4 antibody has been utilized in mouse models, specifically in studies investigating iris neovascularization. For instance, it was employed in a study that assessed the role of angiogenic factors during puncture-induced iris neovascularization, demonstrating its effectiveness in immunoblotting applications to analyze protein expression related to angiogenesis ().

Is this antibody effective for detecting CXCR4 in human tissues?

The CXCR4 antibody has been shown to work effectively in human tissue samples. In a study on pulmonary arterial hypertension, the antibody was used in immunohistochemistry to detect CXCR4 expression in lung specimens from patients, indicating its applicability for human tissue analysis ().

What experimental techniques can I use this antibody with?

This CXCR4 antibody is versatile and can be used in various applications, including Western blotting, immunocytochemistry, immunofluorescence, and immunohistochemistry. It has been cited in multiple studies employing these techniques to investigate CXCR4's role in different biological contexts, such as inflammation and angiogenesis (, ).

Are there any specific conditions or models where this antibody has been validated?

The CXCR4 antibody has been validated in knockout and knockdown models, as well as in various disease models, including those related to pulmonary arterial hypertension and Wallerian degeneration. These studies highlight its utility in understanding the molecular mechanisms underlying these conditions (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) P-Rex1 limits the agonist-induced internalization of GPCRs independently of its Rac-GEF activityCell reportsOctober 28, 2025Baker MJ, Hampson E, Islam P, Moral RP, Maunders EA, Hornigold K, Tsonou E, Malliri A, Hornigold DC, Hubbard RE, et al.Immobilon-P PVDF (Millipore, IPVH00010) following SDS-PAGE. Primary antibodies were CXCR4 (Novus Biologicals, NB100-56437, 1:250), EE (clone Glu-Glu, Babraham Bioscience Technologies, 1:50), EEA1 (BD Biosciences 610456, 1:100),

(2) CXCL12/CXCR4 pathway as a novel therapeutic target for RNF213-associated pulmonary arterial hypertensionScientific reportsNovember 4, 2024Hiraide T, Tsuda N, Momoi M, Shinya Y, Sano M, Fukuda K, Shibahara J, Kuramoto J, Kanai Y, Kosaki K, et al.the manufacturer’s instructions. The primary antibodies were rabbit anti-CXCR4 monoclonal antibody (#NB100-56437, 1:200; Novus Biologicals, Centennial, CO, US) and rabbit anti-alpha smooth muscle actin polyclonal

(3) CXCL12 Neutralizing Antibody Promotes Hair Growth in Androgenic Alopecia and Alopecia AreataInternational journal of molecular sciencesJanuary 30, 2024Zheng M, Kim MH, Park SG, Kim WS, Oh SH, Sung JH.Identify p-STAT3Tyr705,Cell signaling; #9145T,1:100,,DF cell AR,Santa cruz; sc-7305,1:100,1:1000,"DP, DF cell" CXCR4,NOVUS; NB100-56437,,1:1000,DP cell CD8,Santa cruz; sc-1177,1:100,,Mouse skin tissue MHC-I,Santa cruz; sc-55582,1:100,,Mouse

(4) Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchymaLife science allianceOctober 1, 2022Yamamoto Y, Kadoya K, Terkawi MA, Endo T, Konno K, Watanabe M, Ichihara S, Hara A, Kaneko K, Iwasaki N, et al.to detect NETs (Gavillet et al, 2015; Magán-Fernández et al, 2019), CXCR4 (1/100, rabbit from Novus #NB100-56437), and MIF (1/200, rabbit from Thermo Fisher Scientific #PA527343). The following day, sections were

(5) CXCL12 inhibits hair growth through CXCR4Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapieJune 1, 2022Zheng M, Oh SH, Choi N, Choi YJ, Kim J, Sung JH.

(6) Environmental Enrichment Mitigates Age-Related Metabolic Decline and Lewis Lung Carcinoma Growth in Aged Female MiceCancer prevention research (Philadelphia, Pa.)December 1, 2021Queen NJ, Deng H, Huang W, Mo X, Wilkins RK, Zhu T, Wu X, Cao L.Technology #D5H5, 1:1000), CD31 (Cell Signaling Technology #D8V9E, 1:1000), CXCR4 (Novus Biologicals #NB100-56437, 1:500). Blots were rinsed and incubated with HRP-conjugated secondary antibodies (Bio-Rad). Chemiluminescence

(7) Targeting Gi/o protein-coupled receptor signaling blocks HER2-induced breast cancer development and enhances HER2-targeted therapyJCI insightSeptember 22, 2021Lyu C, Ye Y, Lensing MM, Wagner KU, Weigel RJ, Chen S.

(8) UPARANT is an effective antiangiogenic agent in a mouse model of rubeosis iridisJournal of molecular medicine (Berlin, Germany)September 1, 2019Locri F, Dal Monte M, Aronsson M, Cammalleri M, De Rosa M, Pavone V, Kvanta A, Bagnoli P, André H.pSer133,Goat,1:200,WB,Santa Cruz Biotechnology,sc-7978 Anti-CXCR4,Rabbit,1:200,WB,"Bio-Techne Corp., Abingdon, UK",NB100-56437 Anti-FPR1,Goat,1:200,IF,Santa Cruz Biotechnology,sc-13198 Anti-FPR2,Rabbit,1:200,IF,Santa Cruz Biotechnology,sc-66901 Anti-FPR3,Rabbit,1:200,IF,Santa Cruz Biotechnology

(9) Autocrine signals increase ovine mesenchymal stem cells migration through Aquaporin-1 and CXCR4 overexpressionJournal of cellular physiologyAugust 1, 2018Pelagalli A, Nardelli A, Lucarelli E, Zannetti A, Brunetti A.

(10) Macrophage-derived HMGB1 as a Pain Mediator in the Early Stage of Acute Pancreatitis in Mice: Targeting RAGE and CXCL12/CXCR4 AxisJournal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune PharmacologyDecember 1, 2017Irie Y, Tsubota M, Ishikura H, Sekiguchi F, Terada Y, Tsujiuchi T, Liu K, Nishibori M, Kawabata A.

(11) Multifaceted C-X-C Chemokine Receptor 4 (CXCR4) Inhibition Interferes with Anti-Vascular Endothelial Growth Factor Therapy-Induced Glioma DisseminationThe American journal of pathologySeptember 1, 2017Gagner JP, Sarfraz Y, Ortenzi V, Alotaibi FM, Chiriboga LA, Tayyib AT, Douglas GJ, Chevalier E, Romagnoli B, Tuffin G, et al.

(12) A novel in vivo model of puncture-induced iris neovascularizationPloS oneJanuary 1, 2017Beaujean O, Locri F, Aronsson M, Kvanta A, André H.anti-plasminogen; anti-IL6 (cat. no. ab9570, ab154560, and ab6672, Abcam, Cambridge, UK); anti-CXCR4 (cat. no. NB100-56437, Bio-Techne Ltd.); and anti–pan-Actin (cat. no. A2066, Sigma-Aldrich Corp.). Secondary antibody: horseradish

(13) CXCR4 and CXCR7 Mediate TFF3-Induced Cell Migration Independently From the ERK1/2 Signaling PathwayInvestigative ophthalmology & visual scienceJanuary 1, 2016Dieckow J, Brandt W, Hattermann K, Schob S, Schulze U, Mentlein R, Ackermann P, Sel S, Paulsen FP.

(14) CXCR4 regulates migration of lung alveolar epithelial cells through activation of Rac1 and matrix metalloproteinase-2American journal of physiology. Lung cellular and molecular physiologyMay 1, 2012Ghosh MC, Makena PS, Gorantla V, Sinclair SE, Waters CM.

(15) Imaging CXCR4 expression in human cancer xenografts: evaluation of monocyclam 64Cu-AMD3465Journal of nuclear medicine : official publication, Society of Nuclear MedicineJune 1, 2011De Silva RA, Peyre K, Pullambhatla M, Fox JJ, Pomper MG, Nimmagadda S.

(16) HGF upregulates CXCR4 expression in gliomas via NF-kappaB: implications for glioma cell migrationJournal of neuro-oncologyAugust 1, 2010Esencay M, Newcomb EW, Zagzag D.

(17) Molecular imaging of CXCR4 receptor expression in human cancer xenografts with [64Cu]AMD3100 positron emission tomographyCancer researchMay 15, 2010Nimmagadda S, Pullambhatla M, Stone K, Green G, Bhujwalla ZM, Pomper MG.

(18) Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta familyEndocrinologyMarch 1, 2010Hogg K, Etherington SL, Young JM, McNeilly AS, Duncan WC.

(19) Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 geneJournal of immunology (Baltimore, Md. : 1950)February 1, 2009Pacheco-Rodriguez G, Kumaki F, Steagall WK, Zhang Y, Ikeda Y, Lin JP, Billings EM, Moss J.

(20) Hypoxia- and vascular endothelial growth factor-induced stromal cell-derived factor-1alpha/CXCR4 expression in glioblastomas: one plausible explanation of Scherer's structuresThe American journal of pathologyAugust 1, 2008Zagzag D, Esencay M, Mendez O, Yee H, Smirnova I, Huang Y, Chiriboga L, Lukyanov E, Liu M, Newcomb EW.

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CXCR4 Antibody

CXCR4 Antibody

CXCR4 Antibody

CXCR4 Antibody

CXCR4 Antibody - BSA Free from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Questions and Answers

Novus Biologicals, a Bio-Techne Brand

Citations (24)

Reviews

CXCR4 Antibody - BSA Free from Novus Biologicals, a Bio-Techne Brand

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