Fig 1: SpCas9 functionality in primary cells derived from SpCas9 transgenic pigs and chickens. (A) PEFs derived from ear tissue of SpCas9 founder pigs #41 and #42 transfected with a construct carrying a gRNA against B2M or GGTA1 (MOCK-transfected control). (B) PEFs derived from an SpCas9 transgenic pig and transfected with chemically modified gRNA against porcine B2M or P16 (MOCK-transfected control). (C) PADMSCs, PKFs, and PAECs derived from an SpCas9 transgenic piglet and transfected with a construct carrying a gRNA against B2M or GGTA1 (MOCK-transfected control). (D) CEFs derived from an SpCas9-expressing chicken and transfected with RCASBP(A)-EGFP-gRNA or RCASBP(A)-INF-gRNA (MOCK-transfected control). (E) CEFs transfected with a synthetic gRNA against EGFP or B2M (MOCK-transfected control). (F) Splenic CD45+ PMBCs derived from an SpCas9-expressing chicken and transfected with chemically modified gRNA against B2M or CXCR4. Shown is one representative example of three independent replicates.