Anti-Fibronectin 1 antibodies are used for the immunodetection of the protein encoded by the FN1 gene. In humans, the canonical protein has a reported length of 2477 amino acid residues and a mass of 272.3 kDa. Its subcellular localization is in the extracellular matrix and is secreted. Alternative splicing is reported to yield 17 different isoforms for this protein. It is reported to be expressed in the inner limiting membrane and around blood vessels in the retina. It is noted to be involved in angiogenesis and cell adhesion. Post-translational modifications have been described, including glycosylation, protein cleavage, sulfation and phosphorylation.
The fibronectin 1 marker can be used to characterize Type 2 Thymic Fibroblasts and Type 1 Medullary Thymic Epithelial Cells.* Other names for this target antigen include ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, and CIG. Gene orthologs have been identified in the mouse, rat, bovine, frog, chimpanzee and chicken species. Over 2500 citations in the literature describe the use of Fibronectin 1 antibodies in research. Immunohistochemistry is a widely used application for these antibodies. ELISA, Western Blot, and Immunofluorescence are also common applications.
*HuBMAP Human Reference Atlas v1.4