Fibronectin Antibody AF1918 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemFibronectin Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $559.00Supplier Page View Company Product Page
Catalog NumberAF1918
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry,ELISA Capture
ReactivityHuman,Mouse,Ovine,Rat
IsotypeIgG
TargetFibronectin
HostSheep
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ClonalityPolyclonal
ImmunogenHuman plasma-derived Fibronectin
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namefibronectin 1
NCBI Gene AliasesCIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Fibronectin in direct ELISAs. Detects human and rat Fibronectin in Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

What applications has the Fibronectin Antibody been used for in scientific research?

The Fibronectin Antibody has been shown to work in various applications including Western blotting, ELISA, immunocytochemistry, and immunohistochemistry. For instance, it has been utilized in studies to analyze fibronectin expression in lung epithelial cells and in endothelial cells during inflammation models, demonstrating its versatility in cell biology research.

Can this antibody be used to detect fibronectin in different species?

Yes, the Fibronectin Antibody is reactive with multiple species, including human, mouse, rat, and sheep. This broad reactivity allows researchers to apply it in comparative studies across different animal models and human samples.

How has the Fibronectin Antibody been utilized in studies related to epithelial-to-mesenchymal transition (EMT)?

The Fibronectin Antibody has been employed in studies investigating EMT, such as in the context of lung epithelial cells exposed to oxidative stress and radiation. It was used to assess fibronectin levels as a marker of EMT, indicating its role in understanding cellular transitions associated with cancer and tissue remodeling.

What are some specific findings from studies that have used this antibody?

In one study, the Fibronectin Antibody was used to confirm increased fibronectin expression in human retinal pigment epithelium cells undergoing EMT, highlighting its importance in the pathology of proliferative vitreoretinopathy. Additionally, it has been implicated in promoting tumor metastasis through interactions with other proteins, such as B7-H3 in renal cell carcinoma, showcasing its relevance in cancer research.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Metastasis suppressing properties of the cell-surface anchored serine protease prostasin: new functional and mechanistic insights from breast cancerOncogenesisApril 17, 2026Joseph G. Lundgren, Michael G. Flynn, Ani R. Winkler, Brianna N. Rivera, Lauren M. Tanabe, Paul M. Stemmer, Li-Mei Chen, Karl X. Chai, Karin ListDanvers, MA; rabbit anti-FN1 (E5H6X) Alexa Fluor® 488 Cell Signaling, Danvers, MA; sheep anti-fibronectin (AF1918) R & D Systems Minneapolis, MN; rabbit anti-prostasin (PA5-80945) ThermoFisher, Waltham, MA) overnight

(2) An animal component-free bioprocess for synthesizing 3D human matrix scaffolds using mesenchymal stromal cellsFront Cell Dev BiolApril 13, 2026Shaianne N. Stein, Lorena R. BraidBio-Techne, Toronto, ON, Canada, NB300-144), fibronectin (FN, 1: 200, Bio-Techne, Toronto, ON, Canada, AF1918), collagen VI alpha 1 (Col VI, 1: 200, Bio-Techne, Toronto, ON, Canada, NB120-6588), or lysyl oxidase-like

(3) APOE4 promotes cerebrovascular fibrosis and amyloid deposition via a pericyte-to-myofibroblast transitionbioRxivSeptember 16, 2025Braxton R. Schuldt, Dominic Haworth-Staines, Andrea Perez-Arevalo, Diede W.M. Broekaart, Leon Wang, Georgia Gallagher, Grace Rabinowitz, Alison M. Goate, Towfique Raj, Ana C. Pereira, et al.AF806 CD31/PECAM-1,R&D Systems,Cat# BBA7 CD31/PECAM-1,Thermo Scientific,Cat# MA5-29475 Fibronectin,R&D Systems,Cat# AF1918 NG2,Abcam,Cat# Ab255811 PDGFRB,Abcam,Cat# Ab69506 pSmad2,Millipore-Sigma,Cat# AB3849-I Smad2,Cell Signaling Technology

(4) KCNJ16-depleted kidney organoids recapitulate tubulopathy and lipid recovery upon statins treatmentStem Cell Research & TherapyAugust 26, 2024E. Sendino Garví, G. J. J. van Slobbe, E. A. Zaal, J. H. F. de Baaij, J. G. Hoenderop, R. Masereeuw, M. J. Janssen, A. M. van Genderenanti-ZO-1 (#610966, BD Biosciences, Vianen, The Netherlands) diluted 1:200, sheep anti-fibronectin (#AF1918, R&D systems) diluted 1:400, rabbit anti-collagen-I (#ab34719, Abcam, Amsterdam, The Netherlands) diluted

(5) Epithelial-mesenchymal Transition (EMT) and the Effect of Atorvastatin on it in ARPE-19 cellsCell biochemistry and biophysicsJune 1, 2024Mysore Y, Hytti M, Deen AJ, Ranta-Aho S, Piippo N, Toppila M, Loukovaara S, Harju N, Kauppinen A.primary antibody (1:125; Cat. MAB1420, R&D Systems) or sheep polyclonal fibronectin antibody (1:500; Cat. AF1918, Bio-Techne) in 1% BSA and 0.1% Triton-X in DPBS overnight at +4 °C or for 90 min at room temperature,

(6) Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and SheepInternational journal of molecular sciencesMay 24, 2022Michalski D, Reimann W, Spielvogel E, Mages B, Biedermann B, Barthel H, Nitzsche B, Schob S, Härtig W.Billerica, MA, USA",AB749,1:100,Alexa Fluor647 Fibronectin,Sheep,"Bio-Techne; Wies-baden, Germany *",AF1918,1:100,Cy2 GFAP,Guinea pig,Synaptic Systems,173004,1:200,Cy2 Iba,Guinea pig,Synaptic Systems,234004,1:100,Cy2 NeuN,Guinea pig

(7) Fibronectin enhances tumor metastasis through B7-H3 in clear cell renal cell carcinomaFEBS open bioNovember 1, 2021Xie J, Sun M, Zhang D, Chen C, Lin S, Zhang G.ccRCC (Fig. 4A). Our previous study observed that ELISA boards coated with FN polyclonal antibody (AF1918; R&D Systems) can also identify B7‐H3 molecules (data not shown), indicating that B7‐H3 and FN may form

(8) Aminophylline modulates the permeability of endothelial cells via the Slit2-Robo4 pathway in lipopolysaccharide-induced inflammationExperimental and therapeutic medicineSeptember 1, 2021Chen Q, Zhou X, Hou R, Zhou Z, Wang Z, Chen Y, Weng J, Xu J.Pharmaceutical Co., Ltd. The Robo4 (cat. no. MAB50041), VE-cadherin (cat. no. AF1002), fibronectin (cat. no. AF1918) and integrin (cat. no. AF2045) primary antibodies were purchased from R&D Systems, Inc. Alexa Fluor

(9) Glycosylated fibronectin as a first trimester marker for gestational diabetesArchives of gynecology and obstetricsOctober 1, 2020Alanen J, Appelblom H, Korpimaki T, Kouru H, Sairanen M, Gissler M, Ryynanen M, Nevalainen J.R&D Systems, Abingdon, UK). Sheep polyclonal Eu-N1-labeled Anti-hFibronectin antibody was used as a tracer (AF1918, R&D Systems, Abingdon, UK). To reduce unspecific binding in the assay for glycosylated (SNA-reactive) Archives

(10) ARID1A loss in neuroblastoma promotes the adrenergic-to-mesenchymal transition by regulating enhancer-mediated gene expressionScience advancesJuly 1, 2020Shi H, Tao T, Abraham BJ, Durbin AD, Zimmerman MW, Kadoch C, Look AT.(Cell Signaling Technology, #9585S and #5741S), and anti-FN1 sheep polyclonal antibody (R&D Systems, #AF1918). Immunoprecipitation was performed as described previously (12). Cells were first lysed in buffer A

(11) A NOTCH feed-forward loop drives reprogramming from adrenergic to mesenchymal state in neuroblastomaNature communicationsApril 4, 2019van Groningen T, Akogul N, Westerhout EM, Chan A, Hasselt NE, Zwijnenburg DA, Broekmans M, Stroeken P, Haneveld F, Hooijer GKJ, et al.(9542), and FLAG (2368) were obtained from Cell Signaling Technology. Other primary antibodies were FN1 (AF1918, R&D systems), PHOX2A (sc81978), PHOX2B (sc376997, Santa Cruz), α-tubulin (T5168, Sigma) and β-actin

(12) Stromal Fibroblasts Drive Host Inflammatory Responses That Are Dependent on Chlamydia trachomatis Strain Type and Likely Influence Disease OutcomesMBioMarch 19, 2019Jolly AL, Rau S, Chadha AK, Abdulraheem EA, Dean D.CA), speciﬁc for columnar epithelial cells at a 1:100 dilution. Sheep anti-human ﬁbronectin antibody AF1918 was used to detect ﬁbroblasts (R&D Systems) at 1:300. Secondary Alexa Fluor 488 goat anti-rabbit (A11008;

(13) Fibrillin-2 is a key mediator of smooth muscle extracellular matrix homeostasis during mouse tracheal tubulogenesisThe European respiratory journalMarch 1, 2019Yin W, Kim HT, Wang S, Gunawan F, Li R, Buettner C, Grohmann B, Sengle G, Sinner D, Offermanns S, et al.immunostaining and 1:1000 for Western blots, ab21600; Abcam, Cambridge, UK); sheep anti-Fibronectin (1:1000, AF1918; Novus Biologicals, Centennial, CO, USA); rabbit anti-Fibronectin (1:500 for immunostaining and 1:1000

(14) Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma RadiationOxidative medicine and cellular longevityJanuary 1, 2019Acheva A, Haghdoost S, Sollazzo A, Launonen V, Kämäräinen M.Cell Signaling, Denver, MA, USA) for overnight at 4°C; and anti-ﬁbronectin 1 : 20 (polyclonal sheep anti-human #AF1918, R&D Systems, Minneapolis, MN, USA), again overnight at 4°C. The incubation with secondary Alexa-488-conjugated

(15) Adrenergic to mesenchymal fate switching of neuroblastoma occurs spontaneously in vivo resulting in differential tumorigenic potentialJournal of Molecular and Clinical MedicineDecember 20, 2018Maria C. Lecca, Marianne A. Jonker, U. Kulsoom Abdul, Asli Küçükosmanoglu, Wessel van Wieringen, Bart A. WestermanCell Signaling #5741; Slug (C19G7), Rabbit mAb Cell Signaling 9585; Fibronectin antibody, R&D systems AF1918; DBH (Cell Signaling 8586s); PHOX2B (Santa Cruz sc-376997); TH (Santa Cruz sc-25269); MYCN (Pharmingen

(16) Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord InjuryFrontiers in bioengineering and biotechnologyJanuary 1, 2018Vishwakarma SK, Bardia A, Lakkireddy C, Paspala SAB, Khan AA.

(17) Multimerin-2 is a ligand for group 14 family C-type lectins CLEC14A, CD93 and CD248 spanning the endothelial pericyte interfaceOncogeneNovember 2, 2017Khan KA, Naylor AJ, Khan A, Noy PJ, Mambretti M, Lodhia P, Athwal J, Korzystka A, Buckley CD, Willcox BE, et al.clone DM1A (Sigma-Aldrich, Gillingham, UK, #T9026), anti-fibronectin sheep polyclonal (R&D Systems, #AF1918), anti-CD31 rat clone MEC13.3 (BD Bioscience, Oxford, UK, #565629), mouse IgG isotype control (Thermo Fisher Scientific

(18) Neuroblastoma is composed of two super-enhancer-associated differentiation statesNature geneticsAugust 1, 2017van Groningen T, Koster J, Valentijn LJ, Zwijnenburg DA, Akogul N, Hasselt NE, Broekmans M, Haneveld F, Nowakowska NE, Bras J, et al.

(19) Intracellular mechanisms of molecular recognition and sorting for transport of large extracellular matrix moleculesProceedings of the National Academy of Sciences of the United States of AmericaOctober 11, 2016Ishikawa Y, Ito S, Nagata K, Sakai LY, Bächinger HP.antibody against Hsp47 (ADI-SPA-470; Enzo Life Sciences), sheep polyclonal antibody against fibronectin (AF1918; R&D Systems), rabbit polyclonal antibody against mouse type I collagen (AB765P; EMD Millipore), goat

(20) Revealing cytokine-induced changes in the extracellular matrix with secondary ion mass spectrometryActa biomaterialiaMarch 1, 2015Taylor AJ, Ratner BD, Buttery LD, Alexander MR.collagen I polyclonal (Millipore, AB765P) and sheep anti-human fibronectin polyclonal (R&D Systems, AF1918). The primary antibody was allowed to incubate overnight at 4°C. Subsequently, samples were washed with

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